BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,005	T→A	D73V (GAT→GTT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,005	0	T	A	98.6%	739.7 / NA	220	D73V (GAT→GTT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (1/1); major base A (103/111); minor base C (2/1); total (106/114)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 220 total aligned reads displayed.

TACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAG  >  NC_000913/4116730‑4117273

tACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGa                                                                                                                                                                                                                                                                            >  2:880351/1‑280 (MQ=255)

      aCAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATTGATAGGCCTGCCGGTTTCTTTTTCCCTTACAATAGTTGTTTTACGCTGGTTTGTAATACCGCTAGCTGCCATTTGATCGGAACTGa                                                                                                                                                                                                                                                                       >  2:557585/1‑279 (MQ=255)

        aGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAAc                                                                                                                                                                                                                                                                          >  1:188946/1‑274 (MQ=255)

          cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCg                                                                                                                                                                                                                                                                  >  2:806081/1‑280 (MQ=255)

                  cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTTTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATACGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTGACGTTGGTTCGTAATACCGATAGCTTCAATTTGAACGGAACTGATATTgg                                                                                                                                                                                                                                                                 >  1:361658/1‑273 (MQ=255)

                       cgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAACTGCTCGCAGATTTCTGCGGTACGACGGCACTGGCAGCCAATGGCGTAATGGATCGGCTGGCCGGCTTCTTTTTCCCAGACAATAGCGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGc                                                                                                                                                                                                                                                          >  1:438912/1‑275 (MQ=255)

                         tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                                                                                                        <  3:550032/275‑1 (MQ=255)

                           cACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCGGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTc                                                                                                                                                                                                                                                >  2:9803/1‑280 (MQ=255)

                               cTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCACCAC‑t                                                                                                                                                                                                                                                  >  1:22365/1‑274 (MQ=255)

                                tCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGTCTTGCCGGTTTCTTTTTCCCAGACAATATTGGTTTCACGCTAGTTCGTAGTACCGATAGCTGCACTTTTAACGGAACTGATATCGGATTTCGCCAGCAC‑TCCTAc                                                                                                                                                                                                                                             >  2:1035204/1‑278 (MQ=255)

                                     cgagCATTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGAGGTACTACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTAAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTg                                                                                                                                                                                                                                      <  2:419508/279‑1 (MQ=255)

                                            tCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATATTGGTTTCCCGCTGGTTTGTAATACCGATAGCTGCACTTTGACCGGCACTGATATGGTCTTTTGCCAGCAC‑TTTTACCAGCGTgg                                                                                                                                                                                                                                     >  3:462553/1‑274 (MQ=255)

                                                   ggTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                                                                                                        <  4:193174/249‑1 (MQ=255)

                                                    gaggAGGAACCACTGCACATAGGGGACAAAAAAGTAAGGGTAAATAACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                       <  4:710082/277‑1 (MQ=255)

                                                    gTCAAGAATCCACTTCCCGTTGGTGCCAGAAAATTCCGGGTCAATAACCAGACCGGTATTGCTGCGGATAGAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                       <  4:806081/280‑1 (MQ=255)

                                                       gAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACACTAGTGGTTTACCGCTGGTTCGTAATACCGATAGCGTCAATTTGAACGGAACTGATATCGGCTTT‑GCCCACACTTTCTACCCACGTGGGGCTTCTGGTTGccccg                                                                                                                                                                                                                    >  4:34344/1‑278 (MQ=255)

                                                         ggATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                       <  1:483864/275‑1 (MQ=255)

                                                         ggATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                  <  4:627643/280‑1 (MQ=255)

                                                         agATCAAGTTCCCGTTGGTTCAAAAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCTGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGGCGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                  <  4:890544/279‑1 (MQ=255)

                                                              cacttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTACTGCGGATATAATCTTCTAAACCGTCACGTTTTACATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACACTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATTCCGATAGCTGCAATTTGCACGGACCTGATACCTGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGTTGGCCCAGAt                                                                                                                                                                                                                  >  1:865524/1‑275 (MQ=255)

                                                              cacttcacttGTGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAGTGCTGCGTATATAATCTTCTAAACCGTCACGTTTTAGATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACTATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCGGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTGTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                  <  3:555762/275‑1 (MQ=255)

                                                               acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCGTAATACCGGTAGCTGCAATTTGCACGGAACTGATCTCGTCTTTCGCCAGACC‑TCCTACCATCGTGTCGCTTTGGGTGGCCCGGGTTGCCAt                                                                                                                                                                                                            >  4:345099/1‑280 (MQ=255)

                                                               acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATCCCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTTGATCTTTGGTTGGCCCAGGttttcgt                                                                                                                                                                                                            >  4:521384/1‑276 (MQ=255)

                                                               acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt                                                                                                                                                                                                                 >  3:218322/1‑275 (MQ=255)

                                                                cttcacgtTGTGGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                           <  2:404650/280‑1 (MQ=255)

                                                                   cacttTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                                                                                                                        >  2:312551/1‑280 (MQ=255)

                                                                     cttTGGTGGCAGAAAAGTAGTGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGTTATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                           <  1:1023765/275‑1 (MQ=255)

                                                                      tttGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACGGACAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTGCACGCTGGTTCGTCATCCCGATAGCTGCACTTTGAAAGGAACTGATATCGGTTTTCGTCAGCAC‑TACTACAAGCTTGGAGCTTTTGGTGGGCCAGATTTCCAtt                                                                                                                                                                                                           >  3:700882/1‑274 (MQ=255)

                                                                        tGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTGCTTTTTCACAGACAATAGTGGTTTCACGCTGGTTCGTAATACCTATAGCTGCAATTTGAACGGACCTGATATCGGCTTTCGCCAGCAC‑TTATACCAGCGTGGAGCTTTGTGTGGCCCAGGTTTCCATTGGGTCttg                                                                                                                                                                                                   >  2:145874/1‑280 (MQ=255)

                                                                          gTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCAGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                      <  1:550240/275‑1 (MQ=255)

                                                                               aGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATCCCGATAGCTGCAATTTGAACGGAAATGATATCGGCTTTCGCCAGCAC‑TTCTACCATCGTGTAGCTTTGGGTGGCCCAGATTTCCATTGTGTCGTTTTCTAcg                                                                                                                                                                                            >  4:866361/1‑279 (MQ=255)

                                                                                           gtcgatgcccAGACCGTTATTGCTCCGGATATAATCTTCTAAAACGTCACGCTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                                  <  4:385977/270‑1 (MQ=255)

                                                                                           gTCAATCACCAGACCGGTATTGCTGTGGATATAATCTTCTAAACCGTCACGTTTTAAATTCTCGCAGATTTCTGCGGTACGACGTCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                                  <  4:731846/278‑1 (MQ=255)

                                                                                           gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                                  <  2:235189/278‑1 (MQ=255)

                                                                                              aaTCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCCCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACCATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGAAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGAGTGGTGCTTTTGGTGGCCCAGATTCCCCTTGGTTCGTTTTCTACCCAACCTGGtttcg                                                                                                                                                                               >  4:820097/1‑276 (MQ=255)

                                                                                                     aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGt                                                                                                                                                                            >  1:14914/1‑274 (MQ=255)

                                                                                                      gACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATTGCTGCAATTTGACCGGAACTGATTTCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGTGTGGCCTAGATTTCCATTGGGTCGTGTTCTTCCCCCCCTGGTTTTGGGt                                                                                                                                                                            >  3:9621/1‑273 (MQ=255)

                                                                                                                        tataATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                   <  2:549771/280‑1 (MQ=255)

                                                                                                                               ttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGGGGTACGGCGGCACTGCCAGACAATTGCGTTATAGATAGGCTTGCCGGTTTCTTGTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                 <  1:1005822/275‑1 (MQ=255)

                                                                                                                               ttcacaaCCATCACGTTATTACTGCTAGCTGAGTTCTGCGGTACTGCGGCAATGCCACACAACGGCGTTATAGATAGGCTTGCCGGTTGCTTTTTCCCAGACAATAGTGGTTTCACGCTTGTTCGTAACACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGTTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                 <  3:80708/270‑1 (MQ=255)

                                                                                                                                tctAAACCGTAACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGATAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                            <  2:516753/279‑1 (MQ=255)

                                                                                                                                gctAAACCGTCACGTTTTAAATGCTCGCAGAGTTCTGCGGAACGAGGGCACTGCCAGACAATGGCGTTATAGATGGGCTTGCCGGTTTCGTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAGTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                 <  3:266077/273‑1 (MQ=255)

                                                                                                                                 ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCCAATTTGCTCTTCGACACTCTAAt                                                                                                                                          >  2:195789/1‑280 (MQ=255)

                                                                                                                                   aaaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTTGCTCAAATTCGGGCTGCGACACGc                                                                                                                                              >  1:821886/1‑274 (MQ=255)

                                                                                                                                     cccttcccGTTTTAAAGGTTCGCAGATTTCTGGGGTACGACGGTACTGCCGGACAATGGCGTTATAGTTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                            <  3:399518/267‑1 (MQ=255)

                                                                                                                                      ccGTCACGTTTTAAAGGTTCGCGGATTTCTGCGGTACGCCTGCACTGCCGGCCAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                                                                                                     <  4:299882/280‑1 (MQ=255)

                                                                                                                                        gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTTCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                                                                                                                   <  2:300092/280‑1 (MQ=255)

                                                                                                                                         tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACCCGCTAATTATATTg                                                                                                                                   >  2:962290/1‑279 (MQ=255)

                                                                                                                                         tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTg                                                                                                                                   >  2:850829/1‑279 (MQ=255)

                                                                                                                                         tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCACAGATTTCCATCTGGTCGTTTTCTACCCAACCGGGTTTTGGGTAGATTTGCTCAAATTTGTGCTGCGACTCGTCAATgt                                                                                                                                        >  1:400795/1‑273 (MQ=255)

                                                                                                                                                      gCTCGCAGATTTCTGCGGTAAGACGGCACTGACAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                          <  1:780453/275‑1 (MQ=255)

                                                                                                                                                       cTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGCATTTGGCATCTTGa                                                                                                                          >  3:744877/1‑274 (MQ=255)

                                                                                                                                                         cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCTATAGCTGCAATTTGAACGGAACTGACATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCACACTGGTTTTGGGTAGCTTTGCGTCAATTCGCGCTGCTCACCGCTAATTCTCTTGGCACCGTGTTCCGttgc                                                                                                                  >  4:431863/1‑278 (MQ=255)

                                                                                                                                                         cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAAAGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                                                                                                  >  2:779707/1‑280 (MQ=255)

                                                                                                                                                                   tGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACCCGCTAAGGATATTGGCATCGTGCTCCTTTACGAAcg                                                                                                             >  3:989600/1‑275 (MQ=255)

                                                                                                                                                                   tGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTCATGTAATTTGCCTCGTTATCCATTACGActg                                                                                                             >  3:289910/1‑273 (MQ=255)

                                                                                                                                                                       gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                                         <  3:188946/275‑1 (MQ=255)

                                                                                                                                                                       gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                                         <  1:9621/275‑1 (MQ=255)

                                                                                                                                                                             cggagcTGACAGACAATGGCGTTCTAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCTTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                    <  1:967113/269‑1 (MQ=255)

                                                                                                                                                                                cACTGCCAGACAATTGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACATGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                                                <  1:940315/275‑1 (MQ=255)

                                                                                                                                                                                  cTGCCCGACAGTGGCGTTAGAGGTAGGCTTCCCGGTTTCTTTTTCCCAGTCAATAGTGGTTTCACGCTGGTTCATAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                                              <  3:843051/275‑1 (MQ=255)

                                                                                                                                                                                   tGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGTTTTGCTCAAATTCCCTCTGCGACACGCTAAAGATCTTGGCATCGTGATCCATACTGACTGCGCGGGCGCTGTGGATGccc                                                                                         >  2:662488/1‑279 (MQ=255)

                                                                                                                                                                                     ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCCTTGGGTGGCCCAGCGTTCCATCGGGTCGTTTTCTACACAACATGGTTTTGGGTCTACTTGCTCAAATTCCCGCGTCGACACGATAGTGATATTGGACTCGTTCTCCTGTACCAACGGTCGGGAGCTTGTGgcgcgccg                                                                                       >  4:146430/1‑275 (MQ=255)

                                                                                                                                                                                       aGACAATGGCGTTATAGATTGCCTTGCCGGTTTCTTTTTCCCAGACACTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                                                         <  1:564610/275‑1 (MQ=255)

                                                                                                                                                                                                  ttATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAcc                                                                                                              >  2:728906/1‑243 (MQ=255)

                                                                                                                                                                                                        ctagGCTTGCCGTTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGGTCTTAATACCGATAGCTGCAATTTGAACGGAACTGAGATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGGGCTGGTGGTGCCCTGGTCGAGCGCA‑ACg                                                                         <  1:269625/272‑1 (MQ=255)

                                                                                                                                                                                                         tagGCTTTCCGGTTTCTTTTTCCCAAACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGata                                                                      <  1:289910/275‑1 (MQ=255)

                                                                                                                                                                                                          agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCTTGATCCATTACGACCGCGCGGGAGCTGGTGTTGCCCTGGTCGGGCGCC‑ACGGTCTAtttt                                                                >  4:317066/1‑280 (MQ=255)

                                                                                                                                                                                                          agGCTTGCCGGTTTC‑TTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATAttttt                                                               >  2:265562/1‑280 (MQ=255)

                                                                                                                                                                                                            gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGCCACGCTCATGATATTGGCATCGTGATCTATTACGACCGCGCGGGAGCTGGTGTTGCCCTGGTCGAGCGAA‑AGGatatat                                                                   >  3:616306/1‑275 (MQ=255)

                                                                                                                                                                                                            gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGTTCCATTACGACCGCTCGGGGGCTGGGGGTGCCCTGGGCGAGCGCC‑AAGATAtttttttt                                                              >  4:462379/1‑280 (MQ=255)

                                                                                                                                                                                                               tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATGGTGATCCATTACGCACGGGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCC‑ACGTTATAtttt                                                                >  3:781793/1‑275 (MQ=255)

                                                                                                                                                                                                               tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATCTCCATTGGGTCGTGTTCTACCTAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGAATAGTGATTCATTACGACTGCGCGGGCTCTGGTGGTGCCCTGTTCGGGCGCC‑CCGTTATCtttt                                                                >  3:100776/1‑275 (MQ=255)

                                                                                                                                                                                                                gCCGGTTTC‑TTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAg                                                         <  4:265562/280‑1 (MQ=255)

                                                                                                                                                                                                                 ccGTTTTCTTTTTCCCTGACAACAGTTGTTTCACTTTGTTTCTGAATACCTGTAGCTTGAAGTTGAACGGAACTTATATCGGTTTTCGCCTGCAC‑TTCAGCCAGCGTGGGGCTTTTGGTGGCCCAGTTTTCCATTGGTTCGTGTTTTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCNACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGAAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAg                                                         <  4:558629/280‑1 (MQ=255)

                                                                                                                                                                                                                 ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCCATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATAtttttttt                                                              >  1:1005040/1‑275 (MQ=255)

                                                                                                                                                                                                                  gtgTTTTTTTTTGCCAGGCAATAGTGGTTTATCGCAGGTTCGTAATACCGATTGTTTCAATTTGAACGTAACTGATATCGGATTTAGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGt                                                        <  4:300186/278‑1 (MQ=255)

                                                                                                                                                                                                                       tCTGTTACCCAGACAATAGTGCGTTCAAGCTGGTTCGTAATGCCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGATTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAg                                                         <  1:462553/274‑1 (MQ=255)

                                                                                                                                                                                                                               ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTg                                            <  2:785547/279‑1 (MQ=255)

                                                                                                                                                                                                                                   aCAATTGTGATTTCACGCTTGTTCGTCATACCGATAGCTTCAATTTGAACTGAACTGATGTCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTTCGACCGCGCTGCAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAAtt                                             <  1:700882/274‑1 (MQ=255)

                                                                                                                                                                                                                                    ctagtgttgTTTCGCGCTGTTTCGTCATACCGATAGCTGCAATTTGAACGGAACTGATATTGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                      <  4:962290/272‑1 (MQ=255)

                                                                                                                                                                                                                                    cAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                      <  2:866361/280‑1 (MQ=255)

                                                                                                                                                                                                                                      aTAGTGGTTTCGCGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGCTATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGATTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATGCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                    <  2:113675/280‑1 (MQ=255)

                                                                                                                                                                                                                                      aTAGTGGTTTCACGCCGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATAGCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                    <  2:345099/280‑1 (MQ=255)

                                                                                                                                                                                                                                         tgggTTTCACGCTGGTTCCTAATACCGATAGCTGCAATTTTAACGTAACGGTGATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGTCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                      <  3:649114/273‑1 (MQ=255)

                                                                                                                                                                                                                                             tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTCGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATTTATTTTTTTTCAGTCATGTTTACTTGTTCTGTGCTCATAtt                             >  2:499341/1‑280 (MQ=255)

                                                                                                                                                                                                                                             tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGACCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCCAATTCGGGTTTCGACACGCTAATGCTCTTGGCATCGTCATCAATTAAGACCGAGCGGGGTCTGGGGGTGCCCTGG‑CGCAGGCACACGGAACTCTTTTTTTCATTATTGTTTAATTGTCCCGTAGt                                   >  3:489773/1‑274 (MQ=255)

                                                                                                                                                                                                                                               tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTTGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTCATGATATTGGCATCTTGATCCATTATGCCCGCCGGGGACCTGGTGGTGCCCTGGTCGAGCGCT‑ACGTTATTTTTTTTTTCAGTCATGTTTAATTTTCCCGTTGTCATATTAc                           >  2:934768/1‑280 (MQ=255)

                                                                                                                                                                                                                                               tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCCCTGCGACACGCTAATGATATTGGCACATTGATCCATTATGATCGCGCGGGCGCTGGTGTTTCCCTGGTCGAGCGCA‑ACGATCTATTTTTTTTCAGTCATGTTTAATTGTCGCGTTGTCATTTTaa                           >  4:490336/1‑279 (MQ=255)

                                                                                                                                                                                                                                                      ggTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCagttttaatggaag                    >  2:770490/1‑267 (MQ=255)

                                                                                                                                                                                                                                                       gTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGAGGGCGCTGGTGGTGGCCGGGTCGAGCGCA‑ACACGATATTTTTTTTCAGTCATGTTTAATTGTCCCGTGGTCAGATTagcgcaca                     >  4:702091/1‑271 (MQ=255)

                                                                                                                                                                                                                                                        ttaggaaTACCGTTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCATCCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCt                  <  2:6064/275‑1 (MQ=255)

                                                                                                                                                                                                                                                             aaTACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGATGCGACACGCTAATGATATTGGCATAGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGTGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTACCGTTGTCTTCTTCAATCGACGCTTTTTg             >  2:56226/1‑280 (MQ=255)

                                                                                                                                                                                                                                                              aTACCGATAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGt            <  2:462379/280‑1 (MQ=255)

                                                                                                                                                                                                                                                                    aTAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGtt           >  1:335997/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                    aTAGCTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTCCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGt                                                   >  3:514993/1‑235 (MQ=255)

                                                                                                                                                                                                                                                                        cTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAg  <  2:1008221/280‑1 (MQ=255)

                                                                                                                                                                                                                                                                        cTGCAATTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTCCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTaa                                               <  1:514993/235‑1 (MQ=255)

                                                                                                                                                                                                                                                                             aTTTGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAg  <  3:335997/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                              tttGAACGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAg                                                                                 >  1:725573/1‑196 (MQ=255)

TACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCAC‑TTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCA‑ACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAG  >  NC_000913/4116730‑4117273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`