Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,330,415	Δ1 bp	5.7%	intergenic (+19/+167)	dacB → / ← obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,415	0	A	.	5.7%	242.0 / 7.9	70	intergenic (+19/+167)	dacB/obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base A (28/38);  new base . (2/2);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

GCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATC  >  NC_000913/3330270‑3330553                                                                                                                                                  |                                                                                                                                            gCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACAt                                                                                                                                          >  3:213973/1‑149 (MQ=255)   aaCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGGAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGCACAATTAGTCAAAAAGAAACCCCCGCACATg‑g                                                                                                                                       >  3:499341/1‑149 (MQ=255)    aCGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGAGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑Gc                                                                                                                                      >  2:691852/1‑149 (MQ=255)        aTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCggt                                                                                                                                  <  4:515013/149‑2 (MQ=255)        aTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCggg                                                                                                                                  <  4:652585/149‑1 (MQ=255)        aTGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCggg                                                                                                                                  >  3:313631/1‑149 (MQ=255)             gTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTc                                                                                                                             <  4:297246/149‑1 (MQ=255)                    cAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                      <  2:427777/149‑1 (MQ=255)                     aaTATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt                                                                                                                     <  3:562410/149‑1 (MQ=255)                     aaTATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt                                                                                                                     <  2:696973/149‑1 (MQ=255)                     aaTATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattatt                                                                                                                     <  4:547008/149‑1 (MQ=255)                       tatCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGa                                                                                                                   <  3:241452/149‑1 (MQ=255)                         tCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACa                                                                                                                 <  4:213973/149‑1 (MQ=255)                           tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAGCCCCGGC‑CATGTGCCGGGGTTTcttttt                                                                                                                        >  2:216778/1‑135 (MQ=255)                           tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAGCCCCGGC‑CATGTGCCGGGGTTTcttttt                                                                                                                        <  1:216778/140‑6 (MQ=255)                           tttCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGTCaaa                                                                                                               <  1:691852/149‑1 (MQ=255)                                 gCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTgcgc                                                                                                         >  2:207968/1‑149 (MQ=255)                                      gCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                    >  4:798751/1‑149 (MQ=255)                                        aGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa                                                                                                                            >  1:704446/1‑123 (MQ=255)                                        aGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa                                                                                                                            <  2:704446/123‑1 (MQ=255)                                         gTAGAACCTCCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGa                                                                                                                          >  3:9827/1‑124 (MQ=255)                                         gTAGAACCTCCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGa                                                                                                                          <  4:9827/124‑1 (MQ=255)                                                cTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                      >  4:276138/1‑121 (MQ=255)                                                cTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                      <  3:276138/121‑1 (MQ=255)                                                     gATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                     <  3:772974/149‑1 (MQ=255)                                                      aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGt                                                                                                             >  3:41711/1‑124 (MQ=255)                                                      aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGt                                                                                                             <  4:41711/124‑1 (MQ=255)                                                          gCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                                                >  1:710788/1‑149 (MQ=255)                                                             tAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACg                                                                             >  2:715666/1‑149 (MQ=255)                                                              aaTCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGc                                                                            <  3:798751/149‑1 (MQ=255)                                                                  gCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTg                                                                                                     >  1:727900/1‑120 (MQ=255)                                                                  gCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTg                                                                                                     <  2:727900/120‑1 (MQ=255)                                                                      tATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                    <  1:159782/149‑1 (MQ=255)                                                                         tCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                 <  1:198643/149‑1 (MQ=255)                                                                            gTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCtt                                                                         <  4:612080/138‑1 (MQ=255)                                                                            gTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCtt                                                                         >  3:612080/1‑138 (MQ=255)                                                                                gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa                                                                                                                            <  4:253462/83‑1 (MQ=255)                                                                                gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCa                                                                                                                            >  3:253462/1‑83 (MQ=255)                                                                                gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                          >  3:85569/1‑149 (MQ=255)                                                                                    gTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt                                                      <  2:789219/149‑1 (MQ=255)                                                                                             gCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                             >  1:776482/1‑149 (MQ=255)                                                                                                  ttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                 <  3:437492/124‑1 (MQ=255)                                                                                                  ttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                 >  4:437492/1‑124 (MQ=255)                                                                                                  ttGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata                                        <  2:78700/149‑1 (MQ=255)                                                                                                   tGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat                                       <  3:79501/149‑1 (MQ=255)                                                                                                     tataAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTg                                     >  3:291058/1‑149 (MQ=255)                                                                                                        aaaGCTATTTATCAGAACAATTAGTAAAAAAGAAACCCCAGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa                                  <  4:499341/149‑1 (MQ=255)                                                                                                         aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAt                                 <  4:85569/149‑1 (MQ=255)                                                                                                          aGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa                                  >  3:285534/1‑147 (MQ=255)                                                                                                          aGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa                                  <  4:285534/147‑1 (MQ=255)                                                                                                           gATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAc                                           <  4:704332/137‑1 (MQ=255)                                                                                                           gATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAc                                           >  3:704332/1‑137 (MQ=255)                                                                                                                  aTCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa                        <  4:313631/149‑1 (MQ=255)                                                                                                                    cAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTAt                                                                                                <  1:233980/75‑1 (MQ=255)                                                                                                                    cAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTAt                                                                                                >  2:233980/1‑75 (MQ=255)                                                                                                                     aGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc                     >  3:416117/1‑149 (MQ=255)                                                                                                                      gAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGcc                    >  3:297474/1‑149 (MQ=255)                                                                                                                      gAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGcc                    <  3:685775/149‑1 (MQ=255)                                                                                                                       aaCAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCt                   <  1:715666/149‑1 (MQ=255)                                                                                                                              aGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGc            <  4:291058/149‑1 (MQ=255)                                                                                                                              aGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGc            <  2:710788/149‑1 (MQ=255)                                                                                                                              aGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGc            >  3:323160/1‑149 (MQ=255)                                                                                                                                tCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGt          >  4:304673/1‑149 (MQ=255)                                                                                                                                 cAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTa         <  1:207968/149‑1 (MQ=255)                                                                                                                                  aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATa               <  2:135633/142‑1 (MQ=255)                                                                                                                                  aaaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATa               >  1:135633/1‑142 (MQ=255)                                                                                                                                   aaaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATTCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAgc       >  4:118454/1‑149 (MQ=255)                                                                                                                                       gAAGCCCCGGC‑CATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc                     <  2:86444/131‑1 (MQ=18)                                                                                                                                       gAAGCCCCGGC‑CATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGc                     >  1:86444/1‑131 (MQ=18)                                                                                                                                        aaaCCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATc  <  2:776482/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                            GCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATC  >  NC_000913/3330270‑3330553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑