Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,113,544	G→A	7.2%	intergenic (+138/‑35)	opgH → / → yceK	OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,113,544	0	G	A	7.2%	175.2 / 2.6	55	intergenic (+138/‑35)	opgH/yceK	OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist/outer membrane integrity lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (28/23);  new base A (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCC  >  NC_000913/1113402‑1113682                                                                                                                                               |                                                                                                                                            aaTAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                       >  4:563958/1‑149 (MQ=255)    aaaaaCGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCgg                                                                                                                                    >  4:47402/1‑149 (MQ=255)                gCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑Attttt                                                                                                                            <  1:504119/144‑1 (MQ=255)                gCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑Attttt                                                                                                                            >  2:504119/1‑144 (MQ=255)                      tGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAagag                                                                                                                 <  3:281499/149‑1 (MQ=255)                         gcTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTa                                                                                                              >  2:712280/1‑149 (MQ=255)                              gCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCAAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAagag                                                                                                                 <  3:122075/141‑1 (MQ=255)                              gCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCAAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAagag                                                                                                                 >  4:122075/1‑141 (MQ=255)                                    cAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGtt                                                                                                   <  3:563958/149‑1 (MQ=255)                                       gCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTaa                                                                                                >  3:428501/1‑149 (MQ=255)                                           aCATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                       >  2:361958/1‑107 (MQ=255)                                           aCATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGc                                                                                                                                       <  1:361958/107‑1 (MQ=255)                                             aTCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGt                                                                                                    <  3:786503/139‑1 (MQ=255)                                             aTCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGt                                                                                                    >  4:786503/1‑139 (MQ=255)                                             aTCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                                                          <  1:712280/149‑1 (MQ=255)                                             aTCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTgtgg                                                                                          >  3:167724/1‑149 (MQ=255)                                                 ttCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                                                      >  1:359726/1‑149 (MQ=255)                                                 ttCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAg                                                                                      >  3:279450/1‑149 (MQ=255)                                                    cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGT‑At                                                                                                                                <  1:518405/104‑1 (MQ=255)                                                    cTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCATAACGCCGGT‑At                                                                                                                                >  2:518405/1‑104 (MQ=255)                                                         aTTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATgg                                                                              >  3:559918/1‑149 (MQ=255)                                                             aTTGATTTTGCAAGGCTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTCTGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                                                          >  3:58739/1‑149 (MQ=255)                                                             aTTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGAc                                                                          >  1:612724/1‑149 (MQ=255)                                                                 aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTg                                                                      >  3:615749/1‑149 (MQ=255)                                                                 aTTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTg                                                                      >  3:430607/1‑149 (MQ=255)                                                                  ttttGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGc                                                                     <  4:615749/149‑1 (MQ=255)                                                                      gCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAg                                                                 <  2:359726/149‑1 (MQ=255)                                                                       cAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGt                                                                <  3:47402/149‑1 (MQ=255)                                                                        aaGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTg                                                               >  4:517540/1‑149 (MQ=255)                                                                         aGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTgg                                                              >  3:131693/1‑149 (MQ=255)                                                                                  gggTCGGATGAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCCTGGTGACCTTGCTGAGTGGCTGTGgcag                                                     >  4:689197/1‑149 (MQ=255)                                                                                    gTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagca                                                   <  1:260692/149‑1 (MQ=255)                                                                                    gTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGgcagca                                                   >  2:643526/1‑149 (MQ=255)                                                                                     tCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCat                                                  <  4:167724/149‑1 (MQ=255)                                                                                       ggATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCatta                                                >  3:379157/1‑149 (MQ=255)                                                                                            aGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGt                                           <  4:58739/149‑1 (MQ=255)                                                                                                gTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCa                                       <  4:112122/149‑1 (MQ=255)                                                                                                       ccgcATCAGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATAcc                                <  3:689197/149‑1 (MQ=255)                                                                                                         gcATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCgg                              <  4:733749/149‑1 (MQ=255)                                                                                                              cGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcag                         <  4:559918/149‑1 (MQ=255)                                                                                                              cGGAAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcag                         <  3:517540/149‑1 (MQ=255)                                                                                                               ggCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGACTGTGGCAGCATTATTAGTCGCACTATACCggggcagg                        >  2:408280/1‑149 (MQ=255)                                                                                                                gCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggg                       >  2:475374/1‑149 (MQ=255)                                                                                                                 cAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcagggg                      <  4:428501/149‑1 (MQ=255)                                                                                                                      acaacGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATGATTAGTCGCACTATACCGGGGCAGGGGCATgg                 >  4:654912/1‑149 (MQ=255)                                                                                                                       caacGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGCGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGc                <  3:428488/149‑1 (MQ=255)                                                                                                                           caaccAAAAAAAATAACGGGGTTATGGCGGTAATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAAc             <  4:641041/146‑1 (MQ=255)                                                                                                                               ctaTAAAAATACCGGCGTTATGCCGGT‑ATTTTTTCACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAata        <  3:654912/147‑1 (MQ=255)                                                                                                                                aaTAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAatat       >  2:609177/1‑149 (MQ=255)                                                                                                                                 aTAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAAtatt      >  2:80974/1‑149 (MQ=255)                                                                                                                                  tAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGa                                                                           >  4:184636/1‑79 (MQ=255)                                                                                                                                  tAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGa                                                                           <  3:184636/79‑1 (MQ=255)                                                                                                                                     aaaTACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATcc  >  1:430768/1‑149 (MQ=255)                                                                                                                                     aaaTACCGGCATAACGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc                     >  1:753299/1‑130 (MQ=255)                                                                                                                                     aaaTACCGGCATAACGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCggggcaggggc                     <  2:753299/130‑1 (MQ=255)                                                                                                                                               |                                                                                                                                            AATAAAAACGTAGTTGCCTGATGCGCTACGCTTATCAGGCCTACATCGTTCCTGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGCCAATAAAAATACCGGCGTTATGCCGGT‑ATTTTTTTACGAAAGAGGTATCAAATGCGTTTAATTGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCC  >  NC_000913/1113402‑1113682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑