BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,900,410	A→T	6.8%	P56P (CCT→CCA)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,410	0	A	T	6.8%	360.7 / 13.0	103	P56P (CCT→CCA)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base A (48/48); new base T (4/3); total (52/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 103 total aligned reads displayed.

ACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAA  >  NC_000913/3900264‑3900556

acacCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAgg                                                                                                                                                  >  2:414580/1‑149 (MQ=255)

     aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaa                                                                                                                                             >  2:388663/1‑149 (MQ=255)

     aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaa                                                                                                                                             >  4:531467/1‑149 (MQ=255)

     aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaa                                                                                                                                             >  2:545366/1‑149 (MQ=255)

     aTCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaa                                                                                                                                             >  2:479419/1‑149 (MQ=255)

            ttGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTGGGGCaaaaaat                                                                                                                                        <  3:149166/147‑5 (MQ=255)

            ttGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTGGGGCaaaaaat                                                                                                                                        >  4:149166/1‑143 (MQ=255)

            ttGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCg                                                                                                                                      <  4:213719/149‑1 (MQ=255)

               aaaaaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                   >  4:38268/1‑149 (MQ=255)

                   aaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTc                                                                                                                               <  4:640183/149‑1 (MQ=255)

                   aaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACCCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTc                                                                                                                               <  4:478717/149‑1 (MQ=255)

                       cTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGc                                                                                                                           <  1:651138/149‑1 (MQ=255)

                          gTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGt                                                                                                                        >  4:548370/1‑149 (MQ=255)

                             cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                   <  4:81009/135‑1 (MQ=255)

                             cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                   >  1:86947/1‑135 (MQ=255)

                             cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                   <  2:86947/135‑1 (MQ=255)

                             cAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                   >  3:81009/1‑135 (MQ=255)

                              aGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                                                                                    <  3:38268/149‑1 (MQ=255)

                                gTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCgg                                                                                                                  >  1:14449/1‑149 (MQ=255)

                                 ttGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGc                                                                                                                 <  1:643790/149‑1 (MQ=255)

                                    ccTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCAtt                                                                                                              <  2:631859/149‑1 (MQ=255)

                                     cTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTg                                                                                                             >  4:566888/1‑149 (MQ=255)

                                      tttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGa                                                                                                            >  3:102547/1‑149 (MQ=255)

                                       ttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAc                                                                                                           <  1:545366/149‑1 (MQ=255)

                                       ttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAc                                                                                                           <  1:479419/149‑1 (MQ=255)

                                       ttCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAc                                                                                                           <  1:388663/149‑1 (MQ=255)

                                        tCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAcc                                                                                                          >  1:648021/1‑149 (MQ=255)

                                         cAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                                                                         <  2:141923/149‑1 (MQ=255)

                                          aGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTg                                                                                                        >  3:432301/1‑149 (MQ=255)

                                           ggATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGa                                                                                                       <  2:451938/149‑1 (MQ=255)

                                            gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTGGGGCaaaaaa                                                                                                                                         >  2:466562/1‑111 (MQ=255)

                                            gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTGGGGCaaaaaa                                                                                                                                         <  1:466562/114‑4 (MQ=255)

                                            gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAcc                                                                                                          >  4:458537/1‑145 (MQ=255)

                                            gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAcc                                                                                                          <  3:458537/145‑1 (MQ=255)

                                             aTAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGt                                                                                                     <  4:432301/149‑1 (MQ=255)

                                                ggTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTcac                                                                                                  <  3:566888/149‑1 (MQ=255)

                                                           cGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCa                                                                                       <  1:653380/149‑1 (MQ=255)

                                                              ccAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTa                                                                                                                      >  4:351396/1‑115 (MQ=255)

                                                              ccAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTa                                                                                                                      <  3:351396/115‑1 (MQ=255)

                                                              ccAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTa                                                                                                                      >  4:488337/1‑115 (MQ=255)

                                                              ccAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTa                                                                                                                      <  3:488337/115‑1 (MQ=255)

                                                              ccAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCg                                                                                    >  2:245343/1‑149 (MQ=255)

                                                                 gACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGa                                                                                 >  1:522924/1‑149 (MQ=255)

                                                                 gACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGa                                                                                 <  2:796695/149‑1 (MQ=255)

                                                                   cTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATa                                                                               <  2:522924/149‑1 (MQ=255)

                                                                    tCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAg                                                                              >  3:31774/1‑149 (MQ=255)

                                                                      cTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAg                                                                                                                                  <  4:720311/95‑1 (MQ=255)

                                                                      cTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAg                                                                                                                                  >  3:720311/1‑95 (MQ=255)

                                                                      cTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTg                                                                            <  3:531467/149‑1 (MQ=255)

                                                                         cTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGt                                                                         <  2:648021/149‑1 (MQ=255)

                                                                          ttCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCg                                                                                                                   <  2:617419/106‑1 (MQ=255)

                                                                          ttCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCg                                                                                                                   >  1:617419/1‑106 (MQ=255)

                                                                              tCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCgg                                                                    <  3:297994/149‑1 (MQ=255)

                                                                                tttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggc                                                                  <  3:593623/149‑1 (MQ=255)

                                                                                tttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggc                                                                  >  4:287046/1‑149 (MQ=255)

                                                                                 ttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggct                                                                 <  2:14449/149‑1 (MQ=255)

                                                                                   ggTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTgg                                                                           <  2:677182/137‑1 (MQ=255)

                                                                                   ggTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTgg                                                                           >  1:677182/1‑137 (MQ=255)

                                                                                      cATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGa                                                                                                       <  3:99189/106‑1 (MQ=255)

                                                                                        tCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCtggctggc                                                          >  1:573470/1‑149 (MQ=255)

                                                                                          gggTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTcac                                                                                                  >  2:606280/1‑107 (MQ=255)

                                                                                          gggTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTcac                                                                                                  <  1:606280/107‑1 (MQ=255)

                                                                                          gggTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCat                                                        <  4:102547/149‑1 (MQ=255)

                                                                                           ggTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCata                                                       <  1:392712/149‑1 (MQ=255)

                                                                                             tGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCGTTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATc                                                     >  1:47163/1‑149 (MQ=255)

                                                                                              gAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATcc                                                    >  2:755972/1‑149 (MQ=255)

                                                                                              gAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATcc                                                    <  3:355409/149‑1 (MQ=255)

                                                                                               aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAc                                                                                                           <  4:676288/93‑1 (MQ=255)

                                                                                               aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGAc                                                                                                           >  3:676288/1‑93 (MQ=255)

                                                                                               aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCg                                                   >  2:350437/1‑149 (MQ=255)

                                                                                                 tatTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgct                                                 >  3:140843/1‑149 (MQ=255)

                                                                                                   ttGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctgc                                               >  3:489243/1‑149 (MQ=255)

                                                                                                         ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTg                                                                                            >  3:594295/1‑98 (MQ=255)

                                                                                                         ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTg                                                                                            <  4:594295/98‑1 (MQ=255)

                                                                                                         ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGc                                         <  3:94164/149‑1 (MQ=255)

                                                                                                          gaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGcc                                        >  2:642949/1‑149 (MQ=255)

                                                                                                               ggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                                                                                    <  3:48673/68‑1 (MQ=255)

                                                                                                               ggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                                                                                    >  4:48673/1‑68 (MQ=255)

                                                                                                                   cacccaccACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCa                               <  4:140843/149‑1 (MQ=255)

                                                                                                                      ccaccaCTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGaa                            >  3:442408/1‑149 (MQ=255)

                                                                                                                        accacTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAAt                          <  1:245395/149‑1 (MQ=255)

                                                                                                                             tACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGc                     <  2:392040/149‑1 (MQ=255)

                                                                                                                                  agaCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAAt                          <  2:613935/139‑1 (MQ=255)

                                                                                                                                  agaCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAAt                          >  1:613935/1‑139 (MQ=255)

                                                                                                                                    aCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTATCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAAt              >  2:520476/1‑149 (MQ=255)

                                                                                                                                     cATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATa             <  3:287046/149‑1 (MQ=255)

                                                                                                                                      aTTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAg            >  2:548476/1‑149 (MQ=255)

                                                                                                                                      aTTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAg            >  4:554629/1‑149 (MQ=255)

                                                                                                                                      aTTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAg            >  3:700225/1‑149 (MQ=255)

                                                                                                                                       ttttttGCCCCAGGGGCAACGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGTGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGc           >  2:73948/1‑149 (MQ=255)

                                                                                                                                       tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATa             >  2:508169/4‑147 (MQ=255)

                                                                                                                                       tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATa             <  1:508169/144‑1 (MQ=255)

                                                                                                                                       tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGc           >  3:726382/4‑149 (MQ=255)

                                                                                                                                             gCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCTATTGCCAGCgg     <  4:489243/149‑2 (MQ=255)

                                                                                                                                               cccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTa   >  3:353321/1‑149 (MQ=255)

                                                                                                                                               cccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTa   >  1:315193/1‑149 (MQ=255)

                                                                                                                                                ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGc                 >  2:409643/1‑134 (MQ=255)

                                                                                                                                                ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGc                 <  1:409643/134‑1 (MQ=255)

                                                                                                                                                ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa  >  3:643815/1‑149 (MQ=255)

                                                                                                                                                ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa  >  3:236031/1‑149 (MQ=255)

ACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAA  >  NC_000913/3900264‑3900556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`