| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,259,690 | G→A | 8.5% | intergenic (‑394/+29) | psuK ← / ← fruA | pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,259,690 | 0 | G | A | 8.5% | 180.0 / 5.7 | 59 | intergenic (‑394/+29) | psuK/fruA | pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components |
| Reads supporting (aligned to +/- strand): ref base G (28/26); new base A (3/2); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCC > NC_000913/2259547‑2259838 | gAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAtt > 1:224020/1‑149 (MQ=255) taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGc > 4:381388/1‑149 (MQ=255) tCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCt < 1:206488/149‑1 (MQ=255) tGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGt > 1:86167/1‑149 (MQ=255) gAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAacacac > 4:79611/1‑149 (MQ=255) tAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTttat < 2:446906/149‑1 (MQ=255) cTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACg > 3:64158/1‑149 (MQ=255) atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTC‑CTGTCCTGTAac > 2:15079/1‑123 (MQ=38) atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCA‑GAAATTTCCTGTCCTGTAac < 1:15079/123‑1 (MQ=38) aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGTCAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACt < 4:197969/149‑1 (MQ=255) aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACt > 1:428226/1‑149 (MQ=255) aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACt < 1:494811/149‑1 (MQ=255) aCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTg < 2:329117/149‑1 (MQ=255) cGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGc < 2:431787/149‑1 (MQ=255) gTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCg > 1:315405/1‑149 (MQ=255) tttGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGt < 2:24854/149‑1 (MQ=255) aaGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCAc < 4:47915/149‑1 (MQ=255) gTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTttatt < 4:204524/122‑1 (MQ=255) gTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTttatt > 3:204524/1‑122 (MQ=255) ctttCTACTGAAAGATAAACCCATTTAAATTCATAAAATTACAATCACAAACCGGTCATTCTCTTTGCGCATCTTTAATATTACATTACCGGAAATTT‑CTGCCCTGTCACACATATTTTATTACGCTCCTTCCGCTACTGTGTCCACtt > 4:513571/2‑149 (MQ=255) tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt > 4:109353/1‑149 (MQ=255) tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt < 4:73035/149‑1 (MQ=255) tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt > 4:55926/1‑149 (MQ=255) gAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGtt < 3:505925/149‑1 (MQ=255) aGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCa < 4:338001/149‑1 (MQ=255) aaaCCATTTAAATTCATAAAATTACATTTACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg > 4:203656/1‑149 (MQ=255) aaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAgg > 3:260109/1‑149 (MQ=255) aCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACATGACAGGAAATTT‑CTTCCCTGTAACACACATTTTATTATGCTGCATTCGCTACTGCGTCCCCTTCCGGACGTTTCAGGAAGGc > 4:45231/1‑149 (MQ=255) aCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGc < 1:546082/149‑1 (MQ=255) aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc < 4:248711/123‑1 (MQ=255) aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc > 3:248711/1‑123 (MQ=255) aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATa > 2:430444/1‑149 (MQ=255) tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAg < 4:6466/149‑1 (MQ=255) ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAgg < 3:357603/149‑1 (MQ=255) aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa > 3:148388/1‑149 (MQ=255) ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaaa < 1:219262/149‑1 (MQ=255) cATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAcc > 3:62119/1‑149 (MQ=255) aTAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAccc > 2:438811/1‑149 (MQ=255) aTTACATTCACAAACCGGTCTTTCTCTTTGCGCTCATCAAACGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccac > 3:370236/1‑149 (MQ=255) aTTCACAACCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg < 2:280244/149‑1 (MQ=255) aTTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg > 1:12859/1‑149 (MQ=255) acaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg > 1:571690/1‑120 (MQ=255) acaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg < 2:571690/120‑1 (MQ=255) caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCg > 1:81967/1‑149 (MQ=255) aaCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGc > 2:556891/1‑149 (MQ=255) gTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataa > 1:239597/1‑149 (MQ=255) cATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAAtt < 3:63396/149‑1 (MQ=255) aTTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg > 3:186960/1‑149 (MQ=255) tttGCGCTCATCAAATGTTACAGGGCAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa < 2:153387/149‑1 (MQ=255) tttGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa < 2:275898/149‑1 (MQ=255) tttGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa > 1:575239/1‑149 (MQ=255) gcgcTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGt < 4:348523/149‑1 (MQ=255) gcTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTaa > 2:278380/1‑149 (MQ=255) catcaAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAccc < 1:4321/149‑1 (MQ=255) tGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCg < 2:224020/149‑1 (MQ=255) ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc < 3:504131/149‑1 (MQ=255) ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc > 4:504131/1‑149 (MQ=255) ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc < 1:360185/149‑1 (MQ=255) ttACAGGGCAGAAATTTC‑CTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc > 2:360185/1‑149 (MQ=255) ggacaggaAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCGTAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt > 4:320742/1‑149 (MQ=255) ggaAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcgc > 1:359456/1‑149 (MQ=255) gaAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACTCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGcc > 2:99683/1‑149 (MQ=255) | GAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTT‑CTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCC > NC_000913/2259547‑2259838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |