BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,005	T→G	D73A (GAT→GCT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,005	0	T	G	99.6%	764.9 / NA	226	D73A (GAT→GCT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (102/122); minor base A (1/0); total (103/123)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 227 total aligned reads displayed.

CCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTG  >  NC_000913/4116732‑4117276

  aaaCAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCgg                                                                                                                                                                                                                                                                               <  1:1156537/275‑1 (MQ=255)

        cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTg                                                                                                                                                                                                                                                                          >  1:1148987/1‑274 (MQ=255)

          aTTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ata                                                                                                                                                                                                                                                                      <  3:1031259/275‑1 (MQ=255)

          aTTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑at                                                                                                                                                                                                                                                                       >  3:781817/1‑274 (MQ=255)

                cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGCTATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAATAGTTGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCTGAACTG‑ATATCGGCTTTc                                                                                                                                                                                                                                                             >  2:296643/1‑278 (MQ=255)

                cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGAAAGGCTTGCCGGTTTCTTTTTCCCAGACAGTAGTGGTTTAAAGCTGGTTCGGAATACCGATAGCTTCAATTTGAGCTGACCGG‑ATATCgtgttttgc                                                                                                                                                                                                                                                           >  4:701967/1‑272 (MQ=255)

                  cgacgTGCACGCCCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCtt                                                                                                                                                                                                                                                               >  1:1176181/1‑274 (MQ=255)

                     cgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGc                                                                                                                                                                                                                                                           >  1:868606/1‑275 (MQ=255)

                       tGCACGCTTGCGAGAGCCTTCCACATGGTCTAGGATCCACTTCACTTAGGTGCCAGAAAAGTACGGTGCAATCACCAG‑ACCGGTATTGCTACTGATATAATCTTCTAAACCGTCCCGTTTAAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGTTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGATTCGTAATCCCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCa                                                                                                                                                                                                                                                         <  1:705033/275‑1 (MQ=255)

                       tGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACTGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCa                                                                                                                                                                                                                                                         <  1:797540/275‑1 (MQ=255)

                        gCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCGCGGAATTG‑ATATGGGCTTTCGCCAg                                                                                                                                                                                                                                                        >  1:537242/1‑275 (MQ=255)

                        gCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTCCGTAATCACGATAGCTCAAATTTGAGCGGAACTG‑ATATCGGCTTTCCCCat                                                                                                                                                                                                                                                        >  3:285540/1‑274 (MQ=255)

                               cgcgAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTTATACCGCTAGCTGCAATCTGAACGGAACCT‑TAATCGGTTTTCGGCAGCACTTCt                                                                                                                                                                                                                                                 >  3:595199/1‑275 (MQ=255)

                                  ccgagCCTTCCCCATGGTGCAGATTCCACTTCACTTGGGTTCCAGAAAAGTACGGTTTAATCCCCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                                                                                                         <  4:22988/278‑1 (MQ=255)

                                        tttgccaaTGGTTCAGGGTCCAATTTACTTTTGTGCCAGAAAATTAAGGGTCCA‑CACCCGCACCGGTATTGTTGCGAATTTAAATTTATAAATCGTCACCTTTTAAATGCTCGCAGATTTCTGTGGTACGACGTCACTGCCCGACAATGGGGTTATAGATAGGCTTGCCGGTGTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGTAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                                                                                        <  3:168957/268‑1 (MQ=255)

                                          tCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGTTGGTTCGTAATCCCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTTTCACATCGTggt                                                                                                                                                                                                                                      >  3:210644/1‑274 (MQ=255)

                                              caTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCa                                                                                                                                                                                                                                                         <  3:983225/252‑1 (MQ=255)

                                                    cGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTTCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCCGATTTCTGCGTTACGACGGCACTGCCACACAATGGCGTTATCGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAACACCGCTAGCTGCAATTTGAGCGG‑ACTGTATATCGGCTGTCGCCAGCACTCTTACCAGCGTGGATCtttttggg                                                                                                                                                                                                                            >  3:659690/1‑270 (MQ=255)

                                                       ggATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                         <  3:765791/275‑1 (MQ=255)

                                                            cacttcacttTGGGACCAGAAAAGTACGGGTCAATAACCCG‑ACCGGTATTGCTGCGGATATAACCTTCTAAACCGTCACGTTTTAAATTCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                    <  3:25394/275‑1 (MQ=255)

                                                            cacttcaattTGGTGCCATAAAAGTACTGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                                    <  1:734293/275‑1 (MQ=255)

                                                             acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGCTTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGTCAAGAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATCTGAGCGGAACTG‑ATATCGGCTTTCGCTAGTACTTCTACCATCGTGGAGTGTTGGGTGGCCCACAtt                                                                                                                                                                                                                   >  1:957848/1‑275 (MQ=255)

                                                             acttcacttTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGGGGCTTTGGGTGGCCCCGAtt                                                                                                                                                                                                                   >  3:1072600/1‑275 (MQ=255)

                                                                     ttGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAGCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTAc                                                                                                                                                                                                                                               <  2:500981/239‑1 (MQ=255)

                                                                       ggTGCCAGAAAAGAACGGGGCAAACACCAG‑ACCGGTATTGCTGCGGATATAATCCTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                                                                                                                          <  1:415989/274‑1 (MQ=255)

                                                                        gTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                        <  1:680586/275‑1 (MQ=255)

                                                                        gTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                        <  1:781817/275‑1 (MQ=255)

                                                                            cAGAGAAGTACGGGTCAATAACCAG‑ACCGGTATTACGGCGGATATAAACTTCTAAACCGTCACGTTTTAAATGCACGCAGATTTCTGCGGGACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                                                                                                                                    <  3:1083528/275‑1 (MQ=255)

                                                                                         gTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGTTGGTTCGCAATACCGTTATCCGCCCTTTGAGCGGCACTG‑GTATCCGTTTTCGCCCGCACTTTTACCAACGTGCAGAGTTGGTGTGCCCAGATTTCCCTGTGGTAGGGTTCTATCCTAGCTGGcttt                                                                                                                                                                                  >  4:543306/1‑280 (MQ=255)

                                                                                         gTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtttt                                                                                                                                                                                  >  4:467978/1‑280 (MQ=255)

                                                                                         gTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCGTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTg                                                                                                                                                                                       >  3:112952/1‑275 (MQ=255)

                                                                                                                ctgcGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGGGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCGCaa                                                                                                                                                                  >  1:167789/1‑274 (MQ=255)

                                                                                                                     gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑CTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGAGTGGCCCAGATTTCCATTGGGTCGTGTTATATCCAACCTGGTTTTGGTTAGTTTTGCTTAAATTTGCGCTGc                                                                                                                                                       >  2:708480/1‑280 (MQ=255)

                                                                                                                     gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTCCCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGGCGTGTTATACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGc                                                                                                                                                       >  2:5282/1‑280 (MQ=255)

                                                                                                                      ctataATCTTCTAAACCGGCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgcg                                                                                                                                                           <  1:899730/274‑1 (MQ=255)

                                                                                                                          aatctttctaaCCTTCCCGTTTTAAATGCTCGCAGATTTCGTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  4:237933/270‑1 (MQ=255)

                                                                                                                          aaTCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  2:329872/279‑1 (MQ=255)

                                                                                                                          aaTCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  2:467978/279‑1 (MQ=255)

                                                                                                                            tcttctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGCGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                     <  3:1178216/275‑1 (MQ=255)

                                                                                                                            ctttctGAACCGTACCGTTTTACATGCTTGCAGATTTCTGCGTCACGACGGCACTGCCAGACAATGGCGTTATAGAGAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                     <  1:659690/273‑1 (MQ=255)

                                                                                                                              ttctAACCCTTCACGTTTTAAAGGCTCGCAAATTGCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  1:595199/275‑1 (MQ=255)

                                                                                                                              ttctAAACCGTCACGTTTTAAATGCTCGCATAGTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  3:1148987/275‑1 (MQ=255)

                                                                                                                              ttcgAAACCTTCACGTTTGTAATTCTTGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGAGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  3:479822/275‑1 (MQ=255)

                                                                                                                               tctAAACCGGCACTGTGTAAAGGCTCGCAGATTTGTGCGGTACGACGGCACTGCCAGACAAGGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                              <  4:756202/279‑1 (MQ=255)

                                                                                                                                ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCATATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGTTGAAATTCGCCCTGCGaccc                                                                                                                                                  >  1:1024048/1‑272 (MQ=255)

                                                                                                                                  aaaCCGTAACGTTGTAAATGCTCGAAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                                                                                                                <  1:487013/274‑1 (MQ=255)

                                                                                                                                     ccGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                                             <  1:1124093/274‑1 (MQ=255)

                                                                                                                                        taaCGTTGTAAGAGCTGGCAGGTTGCTGGGGGACGACGGCATGGCAAGACAAAGGGGTAATAGATAGGCTTGCCGGTTTCTTTTGCCCAGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATAGCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                                                                                                    <  4:899591/278‑1 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATGGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCACAGATTTCCATTGGGTCGTGCTCTACCCAACCTGGTTTTGGGTAGATTTGCCCAAATTGCCGCTGCTCCACGCTAATGa                                                                                                                                          >  1:36402/1‑274 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTTTACCAGCGTGGCGCTTTGGGTGGCCCAGTTTTCAATTGGGTCGTGTTCTACCCAACCCGGTTTTGGGTAGATTTGCTCAAATTTGCGCTGGGACCCGCTACTGTTATTg                                                                                                                                     >  2:636959/1‑279 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTc                                                                                                                                                                                                                 >  1:940116/1‑203 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGCTTAAATTCGCGCTGCGACACGCTAATGa                                                                                                                                          >  1:1218382/1‑274 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTTTACCCAACCTGGTTTTGTGTAGATTTGCTTAAATTCGCGCTGCGACACGCTAATGat                                                                                                                                         >  3:837611/1‑275 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt                                                                                                                                                         >  3:670168/1‑259 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTGGGTAGATTTGCGCAAATTCGCGCTGCGAAGCGCGAGTGATATTgg                                                                                                                                    >  2:798753/1‑280 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCACAGATTTCCTTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCCCGCTGCGCCCCGCTAATGATATTgg                                                                                                                                    >  2:231115/1‑280 (MQ=255)

                                                                                                                                        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGACAGACAATGGCGTTATAGATAGGCTTGACGGTTTCTTTTTCCCAGTAAATAGTGGGATCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCAAGAACTTATACAAGCGTTGCGCTTTGGGTTGCACACCTGTCCCTGGGTAAGTGTTCTACGTAACGTGTGTGTGGGTAGCTTTGCCCTCATCCGCGCTTCGACCCTCGCATTATATTg                                                                                                                                     >  4:829293/1‑279 (MQ=255)

                                                                                                                                            gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                                                                                                                                                                             <  3:940116/203‑1 (MQ=255)

                                                                                                                                            gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                                   <  1:837611/261‑1 (MQ=255)

                                                                                                                                                tAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                            <  2:848237/280‑1 (MQ=255)

                                                                                                                                                     tctcgcctagttCTGCGGTACTACGGCACTGCCAGACAAGGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACAAGCTAATGATATTGGCATCGTGa                                                                                                                            <  1:270908/265‑1 (MQ=255)

                                                                                                                                                     gCTCGCAGGTTTCTGCGGGACGAAGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAAGTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                            <  1:1094242/275‑1 (MQ=255)

                                                                                                                                                        cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATcc                                                                                                                         >  1:248802/1‑275 (MQ=255)

                                                                                                                                                           aGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCAATTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                                                 <  4:399580/280‑1 (MQ=255)

                                                                                                                                                                         cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCAACCTGGTTTTGGGTAGATTTGTTCAAATTCGCGCTGCGACACGCTCATGATATGTGACTCGTGATCCAATACgctcgccgggga                                                                                                        >  3:1157698/1‑264 (MQ=255)

                                                                                                                                                                               caaggcaaGACAATGGTGTTTTTGGTAGGCTTGGCGGTTTATTTTGCACAGAACAGAGTGGTTTCACGCTGGTTCGGAATACCGATAGCTGCCATTTGGGCGTAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTATACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                                                  <  3:957848/268‑1 (MQ=255)

                                                                                                                                                                                acagccgtaCAAGGTCGTTTTAGACACGCTGGCCGGTTTCTTTTTCCCAGACTATAGGGGTTTCACGCTGGTTCGTGATACCGATAGCTTCAATTTGAGCGTAACTG‑ATATCGGCTTTAGCCAGCATGTCTACCCGCGTGGTGCTTTGGGTGTCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAACTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                                 <  3:195051/267‑1 (MQ=255)

                                                                                                                                                                                aCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                                 <  1:972328/275‑1 (MQ=255)

                                                                                                                                                                                 cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAAtt                                                                                                                                                               >  1:1074653/1‑212 (MQ=255)

                                                                                                                                                                                 cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGcct                                                                                           >  2:125616/1‑279 (MQ=255)

                                                                                                                                                                                                  tataGATATGCTTGCCGGTTTCTTTTTCCCAGACAATATTGGTATCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑TTACCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGcc                                                                                            <  3:159438/262‑1 (MQ=255)

                                                                                                                                                                                                           gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCACTGGTGGTTCCCTGGTCTAGCCGAACGAtttttttttt                                                                 >  4:363751/1‑280 (MQ=255)

                                                                                                                                                                                                           gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATCTGAGCGGAACTG‑ATATCGGCTCTCGCCAGCTCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCTCGTTCTACCCGACCTGGTTCTGGGTAGATCTGCTTAAATTCGCGCTGCGCCTCGTTAATGATATTCGCATCGAGATCCGTTACGACCGACCGGGAGTTGCTCGTCCTCTGGTCGAGCGCATCgtacta                                                                       >  3:902155/1‑269 (MQ=255)

                                                                                                                                                                                                              tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                                                                                                                >  1:385910/1‑214 (MQ=255)

                                                                                                                                                                                                                 cgtttttttttTCCGTGACACTGGTGGTTTCACGCTGGTTCGTAGTACCGGTAGCTCCAAGTTGAGCTGAACTG‑ATATCGGCTTTCGCCAGCACTGCTACCAGCGTGGAGCTTTGGGTGGCCCGGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                                            <  4:636959/276‑1 (MQ=255)

                                                                                                                                                                                                                  ggTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                            <  3:385910/214‑1 (MQ=255)

                                                                                                                                                                                                                   gTTTCTTTTTCCAAGACAATAGTGGTTTCACGCTGGTTCGTAATACAGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                                          <  2:30629/279‑1 (MQ=255)

                                                                                                                                                                                                                     ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGCTCAAATTCGCGCTGCGACACGCTAATGACATTGGCATCGTGATCCATTAAGACCGCGCGGGAGCTGGTGGTGCCCTGGTAGAGCGCAACGAGATATTTTTTTTCAGTAATg                                                       >  2:187180/1‑280 (MQ=255)

                                                                                                                                                                                                                     ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCCACACGCTAATGATATTGGCATCGTGATCCATTCCGACCGTGCGGGGGCTGGTGGTGCGCCGGTCTAGCGCAACGATATCtttttttgatg                                                            >  3:1225437/1‑271 (MQ=255)

                                                                                                                                                                                                                          tttCCCACAAATGAGTGGTTTCCCGCTGGTTCGTAATACCGATAGCTTCAATTTGAGCGCAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                                  <  2:650832/280‑1 (MQ=255)

                                                                                                                                                                                                                                  tcAATAGTGGTTTCACGCTTGTTCGTAATACCGATGGCTGCAATTTGGGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                                               <  3:430821/274‑1 (MQ=255)

                                                                                                                                                                                                                                   ccctAGTGGTTTCCCCCTGGTTCGTAATTCCTCTAGCTGCAATTTGAGCGTACCTG‑ATATCTGCTGTCGCCAGCACTTCTACCAGCGGGGGGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                         <  4:187180/277‑1 (MQ=255)

                                                                                                                                                                                                                                   cAATAGTTGTGTCACGATGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑GTATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                         <  2:829293/280‑1 (MQ=255)

                                                                                                                                                                                                                                   cAATAGTGGGTTCACGCTGGTGCGTAATACCGTTAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACGTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                         <  2:873520/280‑1 (MQ=255)

                                                                                                                                                                                                                                        gtgagttCCCGCTGGTTATTAATACCTGTGGCTGCACTTTGAGCTGAACTA‑ATATCGGCTTCCGCCGGCCCTTCTACCAGCTTGTAGCTTTGGGTGGCCCCGCTTTCCATTTGGTCGTGTTCTACCCAACCTTGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGGGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                         <  3:1078098/270‑1 (MQ=255)

                                                                                                                                                                                                                                          agTTTCACGCTGGTGCGTAAAACCGATAGCTGCAATTTGAGCGGACCTG‑ATATCGGCTTTCGCCATCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                       <  3:892856/274‑1 (MQ=255)

                                                                                                                                                                                                                                            tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                       <  1:457621/273‑1 (MQ=255)

                                                                                                                                                                                                                                              tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt                                                                                                                                                         >  1:327783/1‑157 (MQ=255)

                                                                                                                                                                                                                                              tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCt                                                                                                                                                         >  3:214390/1‑157 (MQ=255)

                                                                                                                                                                                                                                                 cGCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc                           >  2:176306/1‑280 (MQ=255)

                                                                                                                                                                                                                                                  gCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                     <  1:214390/157‑1 (MQ=255)

                                                                                                                                                                                                                                                  gCTGGTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                                     <  3:327783/157‑1 (MQ=255)

                                                                                                                                                                                                                                                     ggTTCGTAATCCCGATAGGTGCAATTTGAGCGGAACCG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGaa                        <  2:420183/279‑1 (MQ=255)

                                                                                                                                                                                                                                                     ggTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTACTTTTCCCGCGTTCATAttctatccaag                       >  4:857966/1‑271 (MQ=255)

                                                                                                                                                                                                                                                      gTTCGTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc                                           >  1:444369/1‑259 (MQ=255)

                                                                                                                                                                                                                                                          gTAATACCGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                       <  3:444369/259‑1 (MQ=255)

                                                                                                                                                                                                                                                                cgggtatgttgcaTTTGATCGGAACTG‑GTGTCTCCTTTCGCCAGCACTTCTACCCGCGTTGGGCTTTGGGTGGCCCATAGTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTc             <  4:780571/267‑1 (MQ=255)

                                                                                                                                                                                                                                                                ccGATAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTa                                                   >  1:194047/1‑241 (MQ=255)

                                                                                                                                                                                                                                                                    tAGCTGCAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                                               <  3:194047/241‑1 (MQ=255)

                                                                                                                                                                                                                                                                          cAATTTGAGCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAgg       >  3:686493/1‑275 (MQ=255)

CCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG‑ACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTG‑ATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTG  >  NC_000913/4116732‑4117276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`