BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,115,901	G→A	A441V (GCA→GTA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,115,901	0	G	A	94.1%	568.1 / NA	207	A441V (GCA→GTA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (93/99); minor base C (8/4); total (102/105)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

GGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAG  >  NC_000913/4115633‑4116169

ggaTTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCCCCGCTTTCTCTTTCAGCTCGTCGCGGTTCTGCCAGAACCCAACCCCCAGGCCTGCCAGATAGGCAGCCCCCAATACgggta                                                                                                                                                                                                                                                                         >  4:266381/1‑272 (MQ=255)

  attattGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGCACGGAACTCGCCCTCAATCACCGCTTTTCCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTCCGGGATGGCCCGCACCCAATACGGTCACt                                                                                                                                                                                                                                                                       >  3:599902/1‑275 (MQ=255)

        gatggtggCGGGGTTGCCCCCGAACGTTTCGGGACTCCCGGATGCAGCATAAACGCCTCATTCGGCATTGACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTcgcg                                                                                                                                                                                                                                                                  <  1:165335/267‑1 (MQ=255)

           gtgtgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGCGGTTCTGCCAGAACCCAACCGCCAGGCCTGCGACATAGGCCGCACCCCATACGGTGACTTCGCGCACtt                                                                                                                                                                                                                                                             >  2:571514/1‑276 (MQ=255)

              tgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTTACGCTAATTACGCCCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCCCGTCGTGGGTCTTCCATAAGCCAACCCCCAGGCCTGCCAGCTAGGCCGCCCCCAATCCGGTCCCTCCGCCCACTTcc                                                                                                                                                                                                                                                           >  4:645237/1‑275 (MQ=255)

              tgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATCGGCCGCACCCAATCCGGTGACTTCGCGCACTTCCg                                                                                                                                                                                                                                                          >  2:848265/1‑276 (MQ=255)

              tgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCCCCAGGCCTGCGAGATAGGCCGCACCCAAAACGGTGACTTCGCGCACTTcc                                                                                                                                                                                                                                                           >  3:301092/1‑275 (MQ=255)

                cGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCNTCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTCATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTCCTCTTGCGTCTCGTCGAGGTTCTGCCATCAGCCAACCGCCAGGCCTGCGAGCACGGCCACACCCAAGACGGCGCCTTCGGCCCCTTCCgcg                                                                                                                                                                                                                                                        >  4:541428/1‑274 (MQ=255)

                        cccccGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCCCTTCCggccccccca                                                                                                                                                                                                                                                 >  3:445787/1‑267 (MQ=255)

                        cccacGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTTCAGCCCGTCCAGGTTCTGCCAGAAGCCACCCGCCATGCCTGCGAGAGAGGCCGCACCCAACACGGGGACTCCCCCCACTTCAgcgcgccgcac                                                                                                                                                                                                                                                >  4:773352/1‑271 (MQ=255)

                                cttttCCGGATTACCGGATGCCGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCgg                                                                                                                                                                                                                                            <  4:880832/270‑1 (MQ=255)

                                cGTTTCTGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTg                                                                                                                                                                                                                                         <  4:479848/275‑1 (MQ=255)

                                 gTTCCGGGCCTCCCGGATGCTGCATAAACGCTTCATTCGGCGTTTGACTTTTTCTTCGTGTTCTTCCCACGCCGTCGCTCGTTTAGCCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCGGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTg                                                                                                                                                                                                                                         <  3:478205/274‑1 (MQ=255)

                                  tttCGGGAGTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGcc                                                                                                                                                                                                                                       <  2:342793/275‑1 (MQ=255)

                                             ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGCCGGAACTCGCGCCCACTCACCGCGTTCTCTTCCAGCTCGTCGAGGTTCTGCCAGCATCCAACCCCCAGCCCCGCCGGACAGGCCGCCCCCAATCCGCTCCCTCCCCGCCCTCCCCGGCCACCACCGCGGGTGcccaccatcacgc                                                                                                                                                                                                                            >  4:951199/1‑264 (MQ=255)

                                             ccGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCTCTTTCTCTTGAAGCTCGTCGAGGTTCTGCCAGACGCCAACCGCCAGGCCTGCGACATAGGCCGCAACCAATACGTTGACTTCGCGGCCTTCCGGGCGCTCCACGCGGGTGCCGAGAAGATCAGa                                                                                                                                                                                                                           >  2:539243/1‑276 (MQ=255)

                                                          aaccGCTTCATTCCGCACTTACATTAGTCGTCGTGTTCTTCCCACTCCAACTCGCTTTTAACCGTTTTTTTCCAGCCGGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGGCGGAACTCGCTCTCAATCACCGCTTTCTCTTGAAGCTCGTCGGGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATc                                                                                                                                                                                                              <  4:131361/273‑1 (MQ=255)

                                                                 tCATTCGGCATTTACCTTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGaaa                                                                                                                                                                                                        <  2:334754/275‑1 (MQ=255)

                                                                 tCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGTAACTCGCGCTCAATCCCCGCTTTCTCTTGCAGCTCGTCGCGGTTCTGCCAGCAGCCAACCGCCAGGCCTGCGAGCTCGGCCGCACCCCATACGGTGACTTCCCGCCCCTCCGGCCGCTCAACGCGGGTGCCGAACATACCGGACTGGAACTGCCTccgtcca                                                                                                                                                                                                        >  4:64146/1‑269 (MQ=255)

                                                                 tCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCCGAAGCCAACCGCCAGGCCTGCGAGCTAGGCCCCACCCAATCCCGTTCACTCCCCCCCTTCCGGGCCCTCCACGCCGGTGCCGCGACTAACTGACTGGAACGTCATTAGGGAAt                                                                                                                                                                                                       >  4:439194/1‑276 (MQ=255)

                                                                 tCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGaaa                                                                                                                                                                                                        <  3:651017/275‑1 (MQ=255)

                                                                     tCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAggctgtcc                                                                                                                                                                                                     >  1:216379/1‑268 (MQ=255)

                                                                        gCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTNTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGACCTTCCGGGCGCCCCCCGCGGGTGCCGAGAATATCCGACTGGAACGGCAGCGGTACATAGTTTGc                                                                                                                                                                                                >  4:528829/1‑276 (MQ=255)

                                                                                 ttattgggtgtgtTTTTCCCCCGACAACCAGCGTTTAACCACGTTTTTCCTGCCGTCGTAACGGTAATTACGCTCAGTCGCTTCGATGCCTGGACGAAACTCGCGGTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGCTCTGCCAGAAGCCAACCGCCAGGCCTGCGCGATAGGCCGCACCCAATACGGTGACTTCGCACACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTgcgc                                                                                                                                                                                        <  3:383061/266‑1 (MQ=255)

                                                                                  tattCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTCCAGCTCGTCGAGGGTCTGCCAGAAGCCAACCGCCTGGCCTGCGAGATAGGCCGCACCCAATACGGTAACTTCGCGCACCTCCGGGCGCTCAACGCGGGCGCCGAGAATAACGGATTGGCACTCCCTCCGGACACTGTTTTCTTCTGCTcc                                                                                                                                                                                       >  3:670431/1‑275 (MQ=255)

                                                                                   attCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTTCGAGATATGCCGCACCCAATACGGTGACGTCCCGCCCCTCCGGGCGCACAACGCGGGGGCCGTGAATGTCGGACTGGCACTGCACCAGGAAGTTCTTGGCTACTGCGcccc                                                                                                                                                                                     >  4:580398/1‑274 (MQ=255)

                                                                                   attCGTCGTGTTCTTCCCACGCCATCGCACGTTTAACCGCGTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGcca                                                                                                                                                                                      <  1:901575/275‑1 (MQ=255)

                                                                                      cgtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGccacca                                                                                                                                                                                   >  1:109801/1‑275 (MQ=255)

                                                                                        tcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATc                                                                                                                                                                                 <  3:146115/275‑1 (MQ=255)

                                                                                             ttccccccACGCCATCGCGCGTTTAACCGCTTTTTCCCAGCCTGCGTAAAGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCGGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAcgcg                                                                                                                                                                           <  4:937912/271‑1 (MQ=255)

                                                                                              tcttcCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACgcg‑c                                                                                                                                                                         <  2:773352/276‑1 (MQ=255)

                                                                                                   ccACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGCTAGGCGGCACCCAATACGGTGACTACGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATAGGAGTGGAACGGAATCAGGAAATTGTTTGCTCCAGCGCCACCACTCAcgcgacgg                                                                                                                                                                       >  1:764366/1‑270 (MQ=255)

                                                                                                     aCGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCgaga                                                                                                                                                                                                                                   >  3:561450/1‑212 (MQ=255)

                                                                                                     aCGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGccac                                                                                                                                                                                     <  2:519973/258‑1 (MQ=255)

                                                                                                        ccATCGCTCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTTGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGc                                                                                                                                                                <  1:839278/275‑1 (MQ=255)

                                                                                                         cATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAatat                                                                                                                                                                                                                               <  1:561450/212‑1 (MQ=255)

                                                                                                         actCGCGCGTTTGACCGCTTTTTCCCAGCCTGCGTAGCGGTAATTACGCTCAGTGGTGTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAg                                                                                                                                                              <  2:11527/274‑1 (MQ=255)

                                                                                                           tCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGAc                                                                                                                                                            <  2:107942/276‑1 (MQ=255)

                                                                                                            cgcgcgTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGaa                                                                                                                                                                                                                                  >  2:98642/1‑206 (MQ=255)

                                                                                                                      cccGCTTTTTTCCAGCCTGCGGAACCGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCagag                                                                                                                                                 <  2:205729/275‑1 (MQ=255)

                                                                                                                      aCCGCTCTTTTCCAGCCGTCGTAACGGTAATTCCGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCagag                                                                                                                                                 <  2:945604/276‑1 (MQ=255)

                                                                                                                                     ccTGCGTAACGGTAATTACGCACAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGCGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGACAACATCGGACTGGAACTGCCACAGGAAATTGTTTGCCACTGCGCCACCAGCCACGCG‑CCGGGCGCGAAAACGGCTACCCAAGTCGGctagcacgc                                                                                                                                    >  3:740737/1‑266 (MQ=255)

                                                                                                                                         cGTCACGGTAATTACGCCCAGTTGCTTCGATTCCTGGACGGCACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGATTCTGCCACTATCAAACCGCCAGGCCTGCGAGATGCGCCGCACCCCATACGGCGCCTTCGCTCCCTTCCGGGCCCCCAACCCGTGTGCGGAGAGTATTGGTATGTAACCTACTCAGGAATGTGATTCCTCATGTTCACACACCCACGCG‑CCAGGCGTGCACACGGAGACTAGAGTCTGccctactctgctcca                                                                                                                              >  4:715511/1‑263 (MQ=255)

                                                                                                                                         cGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCCTCTGGCAACTGTTTGCTTCTCCGCCACCATCCCAGCG‑CAGGGCGTGCAGACGGATACCAACGTCGGCCGTCACCGCTTc                                                                                                                                >  1:890381/1‑274 (MQ=255)

                                                                                                                                         cGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCACCAGGAAATTGTTTGCTTCTGCGCCCCCATCCCCGCG‑CAGGGCGTGAAGACGGACACCAGATCAGGCCTgacgccctccc                                                                                                                               >  4:207473/1‑265 (MQ=255)

                                                                                                                                         cGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCCCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCACCCACGCG‑CAGGGCGTGCCGACGGATACCAGCGTCGGCCGGCATCCCTTcc                                                                                                                               >  4:872286/1‑275 (MQ=255)

                                                                                                                                            aaCGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGGTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAAGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACt                                                                                                                                                                                            <  2:545987/212‑1 (MQ=255)

                                                                                                                                                 tAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCa                                                                                                                      <  2:644739/276‑1 (MQ=255)

                                                                                                                                                   aTTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCACGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAc                                                                                                                                                                              >  1:272462/1‑219 (MQ=255)

                                                                                                                                                     tACGCTGAGTGGTTTCGGGTCCTGGCCGGAACGCGCGCTCAATCTCCGCTTTCGCGACCAGCTGGGCGAGGGTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCGCCACGCGGGCGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCAcg                                                                                                                    <  3:841185/274‑1 (MQ=255)

                                                                                                                                                        gCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCCCGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGCGCTTCACCGCTCCCAGCACGTCACCCGTc                                                                                                                >  2:895832/1‑275 (MQ=255)

                                                                                                                                                         cTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCGCTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCAGCAGGCAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTCCAGACGGAAACCAGCCTCGGCCTGCATCGCTTCCAGCACGTCGCGCGTCt                                                                                                               >  3:771265/1‑275 (MQ=255)

                                                                                                                                                         cTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCAcg                                                                                                                    >  3:514695/1‑270 (MQ=255)

                                                                                                                                                         cTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCAcg                                                                                                                    <  1:514695/270‑1 (MQ=255)

                                                                                                                                                         cTCAGGGGGTTCGATGCCTGGACGGAACTCGAGCTCAATCACCGCGGTCTCTTGCAGCTCGTCGAGGTTCTGCCAGCGGCCAGCCGCCAGGCATACGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACGTCCGGGCGCTCAACGCGGGTGCAGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTg                                                                                                              <  2:654038/276‑1 (MQ=255)

                                                                                                                                                           cAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCTGGTGCCGAGAATATCGGACTGGAACTCCATCAGGAAATTGTTTGCTACTGCGACACCATCCA‑GCGCCAGGGCGTGGCGAAGAGTAACACAGTCCGCCGGCCTCGCTTCCCGCACGTccaccggaccg                                                                                                             >  4:81324/1‑265 (MQ=255)

                                                                                                                                                           cAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCCCCACCATCCACGCG‑CAGGGCGTGCAGGCGGATACCAGAGTCGGCCTGCCTCGCCCCCAGCCCggcccccggcggat                                                                                                            >  4:294783/1‑263 (MQ=255)

                                                                                                                                                                gTTTCGATGCCTGTACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCa                                                                                                                                                                                                                <  4:562018/172‑1 (MQ=255)

                                                                                                                                                                 tttcaagggcTGCACGGAACTCGCGCTCACTCCCCGCGTTCCCTGGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGAACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCaa                                                                                                      <  4:282692/267‑1 (MQ=255)

                                                                                                                                                                      aTGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGCATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCCGCATCGCTTCCAGCCCGTCACGCGTCTGATAAGCAATAGa                                                                                                  >  1:634559/1‑275 (MQ=255)

                                                                                                                                                                      aTGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACCCGTCCGATAAGCCATCGa                                                                                                  >  3:205592/1‑275 (MQ=255)

                                                                                                                                                                           tGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGACTATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCAGCGCTTCCAGCACGTCACGCGGCTGATAAGCAATAGCCCCCa                                                                                             >  1:655245/1‑275 (MQ=255)

                                                                                                                                                                           tGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATACCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCCCCATCCACGCG‑CCGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGCCTGATAAGCAATAGCCCCCAg                                                                                            >  2:416990/1‑276 (MQ=255)

                                                                                                                                                                             gACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCCGCACGTCCCGCGTCTGATGAGCAATCGACTcgaac                                                                                           >  1:195322/1‑271 (MQ=255)

                                                                                                                                                                                ggAATTCGCGCTCAATCGCCGCGTGCTCTGGCAGCAAGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTAAACGCGGGTGCAGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcg                                                                                       <  2:251319/276‑1 (MQ=255)

                                                                                                                                                                                ggAACTCACGCTCAATCACCGCTTTCTCTTGCAGCCCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcg                                                                                       <  2:913532/276‑1 (MQ=255)

                                                                                                                                                                                 ggacccgcgCTCAATCACCGCTTTCCCTGGCAGCCCGTCGATGTTTGGACAGAAGCCAACCGCCACGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcg                                                                                       <  3:675693/270‑1 (MQ=255)

                                                                                                                                                                                 ggaCTCGCGCTCACACACCGCTTTCTCTGGCGGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGGCTGCGAGATAGGCCGGACCCAATACGGTGACTTCGCGCACTTCCGGACGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcg                                                                                       <  3:890381/273‑1 (MQ=255)

                                                                                                                                                                                 gAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACGGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcg                                                                                       <  3:109801/275‑1 (MQ=255)

                                                                                                                                                                                 gAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCCCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCAGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcg                                                                                       <  1:133933/275‑1 (MQ=255)

                                                                                                                                                                                     ccgccgtCAAGCCCCGCGTGCCCTTCCAGAGCGTCAAGGTTCTGCCAGCAGCCACCCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTcgcgc                                                                                    <  1:113594/268‑1 (MQ=255)

                                                                                                                                                                                        cgatcaatcaaCGCCTTCGCGTGGCGCTCGGCGAGGGTCTGCCAGACTCCAACCGCCAGGCACGCGAGATAGGCCGCACCCAATACGGTGACTTCGATCACTTACGGGCGCACGACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTAACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGtata                                                                               <  4:140449/273‑1 (MQ=255)

                                                                                                                                                                                                     cTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCCGCGTCGCGCGTATAATGTGGGTcgcg                                                                   >  2:373313/1‑275 (MQ=255)

                                                                                                                                                                                                      gttcgcgttcaacTCGTAGAAGTTCTGCAAGAGTCCAACCGCCAGGCCTGCAAGAGAGGCCGCACCCACTACGGTGACTTCGCCCACTTCCGGGCGCTAAACGCGGGGGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGtt                                                                 <  2:902510/264‑1 (MQ=255)

                                                                                                                                                                                                       ttCGCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGATCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGtt                                                                 <  1:670431/275‑1 (MQ=255)

                                                                                                                                                                                                        tctcTTGCAGCGCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGGGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCa                                                               <  2:18849/276‑1 (MQ=255)

                                                                                                                                                                                                        cctctGGCAGCTCGCCGAGGTTCTGCCAGAAGCCCACCGCCCGGCCTGCGACATAGGCCGCACCCAAGACGGTGACTTCGCGCACTTCCGGTCGCTCAACGCGGGTGCCGAGAACATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCa                                                               <  2:439194/275‑1 (MQ=255)

                                                                                                                                                                                                                  cTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGGGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGCCGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCAAGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGGTCACCCCACGAg                                                      >  1:698462/1‑275 (MQ=255)

                                                                                                                                                                                                                    cgtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGTCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCCTCAGGAAATTGTTTGCTCCTGCGCCACCATCCACGCG‑CAGGGCGTTCAGACGGATACCAGAGTCGGCCTGCAGCGCTTCCAGCACGTCACGCGTCGGATAAGCAGTAGCCCACCGCGTCGCGCGGATCACGGGGTTCGCGTTCACCCCActcggc                                                    >  4:335281/1‑270 (MQ=255)

                                                                                                                                                                                                                    cgtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCACCGCGGGTGCCGAGAATATCGGAATGGAACTCCATCAGGAAAGTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGTTCAGCAATGACCTCCAGACGCGGCCGTCTACTGTTGTTAGCGCTCAACCCACGCGt                                                     >  3:556775/1‑274 (MQ=255)

                                                                                                                                                                                                                    cgtcgAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGGCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGt                                                     >  1:617161/1‑274 (MQ=255)

                                                                                                                                                                                                                         ggggtcggCCAGAAGCCAACCGGCAGGCCTGCGAGGTAGGCCGCCCCCAATACGGAGACTTCGCGCCCTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGAcc                                               <  1:435544/268‑1 (MQ=255)

                                                                                                                                                                                                                                     aaGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCCCGTCACGCGGCTGATAAGCAATAGACTCCAGCGCCGCGCGTATAATTTGGTTAGCTTTCACCCACCGAGTCACACCGAAAAGcccccc                                   >  4:969015/1‑275 (MQ=255)

                                                                                                                                                                                                                                         cAACCGCCAGGCCGGCGAGGTAGGCCGCCCCCAATACGGTGACTTCGAGGACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGGTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCcgcgc                               <  2:951199/275‑1 (MQ=255)

                                                                                                                                                                                                                                               ccAGGGCTGCGGGGAAGGCCGCACCCAAGACGGTGACGTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                        <  4:195303/276‑1 (MQ=255)

                                                                                                                                                                                                                                               ccAGGCCTGCGGGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGCCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                        <  2:511161/276‑1 (MQ=255)

                                                                                                                                                                                                                                                cAGGCCTGCGAGATAGGCCGCACACAATACGGGGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                        <  1:251734/275‑1 (MQ=255)

                                                                                                                                                                                                                                                      tGCGGGATGGGCCGCACCCAAGTGGGTGCCTTCGCGCCATTCCGGTCGCTCAACGCCGGGTCCGAGAATATCGGACTGGAAGTGCATCAGGAAATTGTTTGCTACGGCGCCAACATCCACGCG‑CAGGGCGTGCAGACGGATACCAGGGTCGGCCTGGATGGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTcc                   <  1:556775/274‑1 (MQ=255)

                                                                                                                                                                                                                                                      ggCGAGATAGGCCGCACCCAATACGGTCACTCCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTcc                   <  1:3003/273‑1 (MQ=255)

                                                                                                                                                                                                                                                       gCGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCCGACCGAAACCCCCCCCGCCCTCACACGGGccccc                 >  4:404392/1‑274 (MQ=255)

                                                                                                                                                                                                                                                        cGAGATAGGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCAACGAGCCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                 >  3:622925/1‑274 (MQ=255)

                                                                                                                                                                                                                                                        cGAGATAAGCCGCACCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGGGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGt               <  2:133711/276‑1 (MQ=255)

                                                                                                                                                                                                                                                            aTAGGCCGCACCCAATCCTGTGACTTCTCGCACTTCCGGGCGCTCAACGCGGTTGCCGAGAATATCGGACGGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg           <  2:296664/276‑1 (MQ=255)

                                                                                                                                                                                                                                                             tAGGCCGCAGCAAATACGGTGACTTCGCGCCCATCCGGGCGCTCAACGCGGGTGCCGAGAAGATCGGACTGGAACGGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg           <  2:396764/275‑1 (MQ=255)

                                                                                                                                                                                                                                                              gggCCCCACCCCATCGGGGGCCTTCGCGCACTGCCGGGCGCTCAACCCGGGTGCGGGGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTGTAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg           <  3:263653/273‑1 (MQ=255)

                                                                                                                                                                                                                                                                  cccccccAAGACGGGGACTGCGGGCACTGCCGGGGCCTCAACGCGGGTGCAGGGAATATCGGACTGGAACGGAATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAcc     <  4:221766/272‑1 (MQ=255)

                                                                                                                                                                                                                                                                  cccACCGAATACGGTGACTGCTCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACGGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAcc     <  2:274057/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                  cGCACCCAATCCAGTGACATCGCGCACTTCCGTGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCAGCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAcc     <  2:628195/276‑1 (MQ=255)

                                                                                                                                                                                                                                                                    cACCCAATACGGTGACTTCGCGCACTTCCGGTCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCCGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATCTGGTTAGCGTTCCCCCCACGCGTCCGACCGAAAATCGCCCCGCGCGTACACGGGTCCCCGTACGGCGCAca     >  1:729359/1‑273 (MQ=255)

                                                                                                                                                                                                                                                                     aCCCAATACGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGCCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAACCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCa   >  1:859954/1‑275 (MQ=255)

GGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCG‑CAGGGCGTGCAGACGGATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAG  >  NC_000913/4115633‑4116169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`