Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,330,419:1	+T	14.3%	intergenic (+23/+163)	dacB → / ← obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,330,419	1	.	T	14.3%	138.5 / 25.2	42	intergenic (+23/+163)	dacB/obgE	D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base . (20/16);  new base T (3/3);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02

TTGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGAT  >  NC_000913/3330157‑3330685                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                           tgtcatgacgctccAGGTCTGGATCGGGCGGGGGTGAATGGAAAAGCCTCAGCGAAAACCGGTTCGTGGCAGGGGGTATATAACCTGGGGGGATTCATTACCACAGCGAGCGGGAAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTc                                                                                                                                                                                                                                                                 <  4:29917/261‑1 (MQ=255)                      cATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCGTTCTGGCGATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGAGATTTATCAGAACAATTAGGCAAAAAGAAACTCCGGCACAAG‑GCGGGGGCTTCCGACTATTgcaacaggtgcatg                                                                                                                                                                                                                                           >  4:486320/1‑262 (MQ=255)                           ggcgggcgTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAACGTGCGCTTTGttt                                                                                                                                                                                                                                      >  1:104152/1‑275 (MQ=255)                                                           ccGGTTCGTTGCAGGGGGGTAATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGGTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                                                                                      <  2:51256/275‑1 (MQ=255)                                                                                             aTTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                                                                                                                                  >  2:35162/1‑261 (MQ=255)                                                                                                 aTTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa                                                                                                                                                                              <  4:35161/261‑1 (MQ=255)                                                                                                   tcccACAGCGGGCGGGCAACGAATGGGCTTTGTGCAAAATCTTTCTGGCTATGACGTAGAGCCGCCGGATCAGCGTAATCGCCGTATTCCGTTAGTTCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTATTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg                                                                                                                                                              <  2:486321/273‑1 (MQ=38)                                                                                                           cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTTCAAATTCAACAGATTGCAATTCTCTTGTAGGCCTg                                                                                                                                                      >  4:292481/1‑275 (MQ=255)                                                                                                           cgagcgGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTg                                                                                                                                                      >  2:79370/1‑275 (MQ=255)                                                                                                                               tttGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                                   <  4:79369/274‑1 (MQ=255)                                                                                                                                       atatCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa                                                                                                                                                                                                            >  4:461027/1‑193 (MQ=255)                                                                                                                                           cTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                                                                        <  2:461028/193‑1 (MQ=255)                                                                                                                                                                       aTCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAAt                                                                                           <  2:181914/274‑1 (MQ=255)                                                                                                                                                                          aGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTGGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGa                                                                                         >  2:105007/1‑273 (MQ=255)                                                                                                                                                                                     cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACaa                                                                                                                                                                                                                                                    >  7:390993/1‑107 (MQ=255)                                                                                                                                                                                     cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACaa                                                                                                                                                                                                                                                    <  8:390993/107‑1 (MQ=255)                                                                                                                                                                                     cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGC‑CATGTGCCgggg                                                                                                                                                                                                                                                                     >  6:393341/1‑90 (MQ=255)                                                                                                                                                                                     cGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGC‑CATGTGCCgggg                                                                                                                                                                                                                                                                     <  5:393341/90‑1 (MQ=255)                                                                                                                                                                                         ttCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa                                                                                                                                                                                                            >  4:304762/1‑143 (MQ=255)                                                                                                                                                                                             gTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                                                                        <  2:304763/143‑1 (MQ=255)                                                                                                                                                                                              ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAg                                                                                                                                                                                                                                                  >  8:565336/1‑100 (MQ=255)                                                                                                                                                                                              ttAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAg                                                                                                                                                                                                                                                  <  7:565336/100‑1 (MQ=255)                                                                                                                                                                                                 gTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCtt                                                                 >  4:464143/1‑274 (MQ=255)                                                                                                                                                                                                      ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACCAAATCGTGCAAATTCAACATATTGCa                                                                                                                                                                       >  1:136361/1‑167 (MQ=255)                                                                                                                                                                                                      ttttGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATAtt                                                                                                                                                                          >  3:45031/1‑164 (MQ=255)                                                                                                                                                                                                          gAAATCCGTTTGTATAAAGAAAGTTATCNGAACAATTAGTCAAAAAGACACCCCGGGACAGG‑GCCGTGGCTGCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGTATGCGGCGTAAACGCCTTATCCGTCCTACAAAATCGTGCAAATTCAACATATTGCAATtc                                                                                                                                                                   <  3:136361/167‑1 (MQ=38)                                                                                                                                                                                                          gAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCaa                                                                                                                                                                      <  1:45031/164‑1 (MQ=255)                                                                                                                                                                                                             aGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          <  5:973244/77‑1 (MQ=255)                                                                                                                                                                                                             aGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          >  6:973244/1‑77 (MQ=255)                                                                                                                                                                                                                 gTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCCGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCACGTCGTCATc                                                 >  2:500711/1‑274 (MQ=255)                                                                                                                                                                                                                          aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          >  8:1038465/1‑64 (MQ=255)                                                                                                                                                                                                                          aaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGattat                                                                                                                                                                                                                                                          <  7:1038465/64‑1 (MQ=255)                                                                                                                                                                                                                               atTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGAGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCatcatc                                  <  1:12814/275‑1 (MQ=255)                                                                                                                                                                                                                                        aaCAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTc                            >  2:308118/1‑272 (MQ=255)                                                                                                                                                                                                                                             ttAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAAtc                     >  4:358191/1‑274 (MQ=255)                                                                                                                                                                                                                                                     aaaGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTAGCATCAGTGATTAACGCTTGTAAATGAACTTAACGCCTTCTTAGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAgc            >  2:509430/1‑275 (MQ=255)                                                                                                                                                                                                                                                          agccccGGC‑CATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                                                                                                                                                        <  7:367888/48‑1 (MQ=17)                                                                                                                                                                                                                                                          agccccGGC‑CATGTGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGt                                                                                                                                                                                                                                        >  8:367888/3‑50 (MQ=17)                                                                                                                                                                                                                                                           gccccGGC‑CATGTGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                                                                                                                                                                                    >  6:969424/2‑69 (MQ=17)                                                                                                                                                                                                                                                           gccccGGC‑CATGTGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa                                                                                                                                                                                                                    <  5:969424/68‑1 (MQ=17)                                                                                                                                                                                                                                                               cGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCgat  <  2:464144/275‑1 (MQ=255)                                                                                                                                                                                                                                                                ggCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTCGGCCTGATAAGCGTAGCGNATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGNCCCATTCTTCATCCCCTTCTTCATCATCCCNNTCATCCATCTCTTCAAGCTCCCGGCgc   >  3:68868/1‑272 (MQ=37)                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                           TTGCAGTACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGAAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCTGGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCGTTTGTGCAATATCTTTCTGGCTATGCAGTAGAACCTGCGGATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTATAAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATG‑GCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCGTCGTCTTCGTCCCAGTCGTCATCCCAGTCTTCATCATCCTCTTCAGCAATCTCTTCAAGCTGCTGGCGAT  >  NC_000913/3330157‑3330685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑