| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 986,410 | A→C | 5.3% | S191S (TCT→TCG) ‡ | ompF ← | outer membrane porin 1a (Ia;b;F) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 986,410 | 0 | A | C | 5.3% | 741.5 / 22.6 | 208 | S191S (TCT→TCG) ‡ | ompF | outer membrane porin 1a (Ia;b;F) |
| Reads supporting (aligned to +/- strand): ref base A (99/96); new base C (7/5); total (106/102) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01 | |||||||||||
Only 100 of 209 total aligned reads displayed.
ACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGT > NC_000913/986138‑986681 | aCTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATTGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCTCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCCCCGTTAg > 4:437699/1‑274 (MQ=255) cTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAATAGGTTGAGCTTCTTGCAGGTTGGTACGGTCCNCTGCACCATAAGCCCCAACGATACCAAAGCCTTCGTATTCTTAGACGATCGAACACCCACAACCATCTCCGTTaca > 3:116415/1‑272 (MQ=255) cTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGACCG‑GAGTGCAgtg > 1:149682/1‑275 (MQ=255) ttGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCCGCTGCCCCATAAGCCCCAACGATACCAACGCCTTCGTCTTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAttg > 2:515633/1‑274 (MQ=255) aaTTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCg < 2:313195/273‑1 (MQ=255) tACGGGTTTCACCGTAGTTCGCTGCCAGGTAGTTGTTGTTCGCGTCGTACGTAAGACCCGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCaaa < 4:361503/274‑1 (MQ=255) cGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTCCTGAACAGCGAAGTTCAGGCCATCAACCAGACCaaaaaa > 4:142915/1‑275 (MQ=255) cACCGTAGTTCGCTGCCAGGTAGATGGTTTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAg < 2:147214/275‑1 (MQ=255) gTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCTCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGAt < 2:437700/275‑1 (MQ=255) gttgttCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTACAGTGTCACGCTCGTTTTTACACAGGTACTGAACAGCCAAGTTCAGGCCATCAACAGGACAAAAGAGGTTGGAGTTACGAAAGGTAGCAACGCAGCCaa > 2:271143/1‑275 (MQ=255) tACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTCCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaa < 4:199718/275‑1 (MQ=255) gACCAGTAGCCCACTGTTCAGATGTTTTACAGTTGACAAGAGGTTGAGCTTCGTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTGCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCa < 1:84501/275‑1 (MQ=255) cAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCACCAACACGACCAACGAAGAAGTCATCg < 2:244394/275‑1 (MQ=255) gtggCCCACTGTTCAGCTTTTTTACCTTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACCGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCATATTCGAAGCTGATAGAACCGCCAGAACCGTCGCCGTTAGAACT‑GCGTGCAGTGTCACGCACGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTCGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCt < 4:267804/272‑1 (MQ=255) gTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCt < 2:92124/275‑1 (MQ=255) gCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTa < 2:171418/275‑1 (MQ=255) ccaccTGTGCAGCTTGTTTACCTGTGCCAAAAGGTTAGGATGTTTGAAGGTTTGTACGGTCAGCTGAACCATAAGCAACAACGATACCAAAGCCGTCGGATTCGGAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTAt < 4:466742/271‑1 (MQ=255) cACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACGAGCGTgac > 5:211336/1‑146 (MQ=255) tGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAATCCTCCGTATTCGTAGCTGATAGAACCGCCAACACCGTCCCCGTTCGAACT‑GCGTGCAGTTTCACGCTCGTTTTTACCCAGGCACTGAACAGCGAAGTTCAGGCCATAAACCAGACCAAAGAAGTCGGAGTTACGATAGGTAGCAACGCCGACAACACGACAAACGAAGAAGTCATCGCTgattcaagt > 4:377376/1‑267 (MQ=255) tGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAgtgt < 5:280138/148‑1 (MQ=255) cTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAgtgt < 5:326332/141‑1 (MQ=255) cTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAgtgt > 6:326332/1‑141 (MQ=255) tttttACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCt < 6:158860/145‑1 (MQ=255) tttttACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGt < 2:472378/250‑1 (MQ=255) cGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTAcc < 8:506436/148‑1 (MQ=255) cGTTGCCAAGAGGTTGAGCTTCTGGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCt < 1:103853/250‑1 (MQ=255) tggCCAAGAGGTTGGGCTTCTTGCAGGTTGGTACGGTAAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTgg < 2:66131/273‑1 (MQ=255) gtGCCAAGGGGTTGAGCCTCTTTCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTAATCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTgg < 4:504994/274‑1 (MQ=255) aaGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTCTAACg‑g > 5:1119842/1‑115 (MQ=255) aaGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGc > 8:581/1‑120 (MQ=255) aaGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAgg > 5:169947/1‑145 (MQ=255) agagGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAgg < 8:162711/144‑1 (MQ=255) gggTTGGGCTTCTTGAAGGTTGGTTCGGTCAGCTGCACCATAAGGACCAACGATACCAAAGCCTTCGTATTCGTAGCTGAGAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTGTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat < 4:81514/274‑1 (MQ=255) agGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat < 2:377377/275‑1 (MQ=255) aGCTGCTTGAAGGTTGGTACGGTCAGCTGCAACATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGGGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGgtg < 4:35081/274‑1 (MQ=255) tGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGt < 7:1119539/118‑1 (MQ=255) tGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTa < 7:884344/123‑1 (MQ=255) tGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTAAGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCAt > 4:316534/1‑223 (MQ=255) aGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTg < 2:335692/274‑1 (MQ=255) ggTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGcc > 5:40784/1‑148 (MQ=255) tGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATc < 6:352672/148‑1 (MQ=255) aCGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAAc < 5:821136/126‑1 (MQ=255) tCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGa < 6:473832/80‑1 (MQ=255) tCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGAc > 8:951333/1‑148 (MQ=255) cATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGa > 6:869922/1‑117 (MQ=255) cATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGa < 5:869922/117‑1 (MQ=255) gCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGc < 7:1050331/74‑1 (MQ=255) aCCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGt > 2:293091/1‑275 (MQ=255) ccAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGa > 8:346158/1‑102 (MQ=255) ccAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGa < 7:346158/102‑1 (MQ=255) cAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAaaga < 5:1031584/135‑1 (MQ=255) cAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAaaga > 6:1031584/1‑135 (MQ=255) aaCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTAcc < 8:297752/90‑1 (MQ=255) aaCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTAcc > 7:297752/1‑90 (MQ=255) aaCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCAATCAACCAGACCAAAGACGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGACGAAGTCATCGATGTATGCAGTATCCCCACCAAATTCTGGCAGCATATCGTTGTAACCCAGTGCATCCACAACCACACCGAAATTACCGCCGTaaa > 1:99499/1‑274 (MQ=255) aCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCaa > 6:591228/1‑121 (MQ=255) aCGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCaa < 5:591228/121‑1 (MQ=255) cGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAg < 7:754369/124‑1 (MQ=255) aaaGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAgtgt > 8:1077372/1‑65 (MQ=255) aaaGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt < 7:46359/128‑1 (MQ=255) aaaGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt > 8:46359/1‑128 (MQ=255) aaGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGttt < 7:669364/75‑1 (MQ=255) aGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCg > 7:1016562/1‑55 (MQ=255) aGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAgt < 6:551765/61‑1 (MQ=255) aGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg > 6:290763/1‑129 (MQ=255) aGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg < 5:290763/129‑1 (MQ=255) aGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCa > 1:122008/1‑275 (MQ=255) ccTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCaa > 5:154631/1‑118 (MQ=255) ccTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt < 7:168362/124‑1 (MQ=255) ccTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGa < 7:281206/128‑1 (MQ=255) ccTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGa > 8:281206/1‑128 (MQ=255) ttcgtattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAAcc > 8:658436/1‑109 (MQ=255) tattcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat < 4:53239/208‑1 (MQ=255) attcgtaGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg > 7:551936/1‑120 (MQ=255) taGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGAc > 5:755856/1‑148 (MQ=255) gCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAac > 8:841769/1‑141 (MQ=255) tGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGt < 5:478252/108‑1 (MQ=255) aTAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAg < 6:1096583/120‑1 (MQ=255) aTAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAg > 5:1096583/1‑120 (MQ=255) gAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaaga < 6:755856/148‑1 (MQ=255) aCCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCaa > 6:212867/1‑86 (MQ=255) aCCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg > 7:834424/1‑104 (MQ=255) ccGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaaga < 7:159332/145‑1 (MQ=255) cAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACg < 7:999792/106‑1 (MQ=255) cAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACga < 7:592550/138‑1 (MQ=255) cacCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaaga > 6:948618/1‑138 (MQ=255) cacCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACgaaga < 5:948618/138‑1 (MQ=255) ccGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGa > 8:1023404/1‑61 (MQ=255) cGTCGCCGTTAGAACG‑GCGTGAAGTGGCACACTCGTTTTTACCCAGGTACTGAGCGGCGAAGTTCAGGCCATCCACCGGACCAAGGAAGTTGGAGTGACGCTAGGTAGCAACGCCGCCAACACGACCAACGAAAAAGTCATCGCTATATGCAGTATAACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAATAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGAcgc < 4:227688/275‑1 (MQ=255) tCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg < 6:706910/91‑1 (MQ=255) tCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTgg > 5:706910/1‑91 (MQ=255) acGCCGTTCTAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCa < 6:639314/125‑1 (MQ=255) acGCCGTTCTAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCa > 5:639314/2‑126 (MQ=255) cGCCGTTCTAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACga > 8:1119893/1‑129 (MQ=255) gCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCATGCCATCAACCAGACCAAAGAAGtt > 8:778260/1‑87 (MQ=255) ccGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGgtg < 2:200304/180‑1 (MQ=255) gTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGAt < 6:1124719/95‑1 (MQ=255) ttAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGt > 2:491303/1‑274 (MQ=255) tAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTAGCCAGGTACTGAACATCGTAGTTCAGGCCATCAACCAGACCAaaga < 8:701095/78‑1 (MQ=38) tAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATc > 6:820958/1‑148 (MQ=255) | ACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCACTGTTCAGCTTTTTTACCGTTGCCAAGAGGTTGAGCTTCTTGCAGGTTGGTACGGTCAGCTGCACCATAAGCACCAACGATACCAAAGCCTTCGTATTCGTAGCTGATAGAACCGCCAACACCGTCGCCGTTAGAACG‑GCGTGCAGTGTCACGCTCGTTTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAACCACACCGTAGTTACGGCCGTAATCGAAAGAACCAACGTCAGCGTATTTAAGACCCGCGAATGCCAGACGCGTTTTGT > NC_000913/986138‑986681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |