| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,324,839 | G→T | 8.9% | E32* (GAA→TAA) | yciQ → | enhancer of membrane protein expression; putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,324,839 | 0 | G | T | 8.9% | 383.2 / 20.0 | 114 | E32* (GAA→TAA) | yciQ | enhancer of membrane protein expression; putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (46/56); new base T (6/4); total (53/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
AAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTA > NC_000913/1324579‑1325085 | aaaaTCGTTTGGAAATACGGTTAGCTTTGAGTTTACACGGGGCCGATCTCGTGCTAAAACCGCGAACGGTTATGGATCTTACCGGTGGTACGCCGGTCGTAGTGCGTGAAGGCGTAGGTGATTTGAAGCCTTTCTTATAACAAATTGCGTACCACGGATGGAAGACTATGGCAGGAAAGTTTCGCTGCATTTTGCTGTTGATAGCAGAGCTGTTTGTATCATCTCTAAGTAATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAAAAGGGATCTCg < 4:179030/272‑1 (MQ=255) aGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGcc < 2:374725/275‑1 (MQ=255) tAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCCTCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGCATCTCGCTCTTTGATGTTGAAGCCAct > 2:108733/1‑275 (MQ=255) ttGGTTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc < 1:96658/274‑1 (MQ=255) tGATTATTACAGGCGGCTAGTGCGGGGACAAACCGGAAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCa < 4:224872/274‑1 (MQ=255) tCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGGTTGTATCATCTCTAAGGTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATTTTGAAGCCACGCTGCAACCAGacg > 4:486426/1‑273 (MQ=255) cACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTCTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg > 2:192384/1‑275 (MQ=255) gcggcTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTg < 2:215585/275‑1 (MQ=255) acggcTATCTCGGGCAGAAACCGGCAACGGTGATTGAGCGTACCGATGATAGGCCGGTCGAGGTGCGTGAAATCGTAGGTGATGTGAAGCCTTTCATATAACAAATTGCGTTCAACGGATGGAGGACTATGGCAGGGAAGTTTCGCTGAATTTTGGTATTGATAGCAGGACTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTg < 4:131812/274‑1 (MQ=255) ggcTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCt < 2:72116/275‑1 (MQ=255) gATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaa > 4:118190/1‑189 (MQ=255) cTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCa > 2:317738/1‑228 (MQ=255) cTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGaaa > 2:122735/1‑275 (MQ=255) ttACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGaagaag < 2:118191/189‑1 (MQ=255) tACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACATATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATACCTTCTT‑ATGAAGAAGGGATTCCGCNCTTTGATGTTGAAGCCACTCTGCAACCAAATGGGGTGCTCGACATCAAAGAAAAGATTCATTTTCAGGCGAGAAATc > 3:132263/1‑275 (MQ=255) ccGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATg < 4:317737/228‑1 (MQ=255) ccGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCa < 2:289964/275‑1 (MQ=255) ggTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACg > 2:523510/1‑275 (MQ=255) cGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGGAGGGAAGTGTCGCTGCATTTTGCTGTTGATAGAATGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCTCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAc < 2:461512/275‑1 (MQ=255) gTAGGTGATGTGAAGCCTGTCTTAGAACAAATTGCGTTCCCAGGATGGAAGACTATGGCAGGGAAGTCTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAc < 4:192383/274‑1 (MQ=255) gcggTAATGGGAAGTCTTTCTTATAACCAATTGGCTTCCGCGGATGGAAAACTATGGAAGGGAAGTTTTGCTGAAATTTGCGGTCGAGAGCAGGGCAGTTTGTATAATCGCTAAGTTATGCGGGAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAcc < 4:45830/272‑1 (MQ=255) gTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa > 4:191465/1‑204 (MQ=255) tgtgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCaa < 2:191466/204‑1 (MQ=255) gtgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGCTGGGGTGCTAGACATCAAAGAAAATATTAATTTTCAGGCGCTAAATCAGCAGATTAAGCACGGCTTTTACCGTGATTTACAACGACTATg > 4:501179/1‑275 (MQ=255) gAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGa < 5:117897/146‑1 (MQ=255) aaGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga > 5:930147/1‑140 (MQ=255) aaGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 6:930147/140‑1 (MQ=255) aGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACg > 2:507462/1‑265 (MQ=255) gCCTTTTTTTTTCCAAATTGGGTGCCCCGGATGGAAGACAATGGTAGGGAAGTTTCTCTGGATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTAACCACGACTATGGATg < 4:531075/274‑1 (MQ=255) tttCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTa < 4:507461/265‑1 (MQ=255) ttCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGc < 4:122734/274‑1 (MQ=255) ataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGAt < 5:49384/148‑1 (MQ=255) aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga > 5:267712/1‑127 (MQ=255) aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 6:267712/127‑1 (MQ=255) aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGt > 5:907657/1‑148 (MQ=255) aCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGc > 2:212707/1‑137 (MQ=255) tGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTg < 4:523509/275‑1 (MQ=255) tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAgg > 8:876060/1‑118 (MQ=255) tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAgg < 7:876060/118‑1 (MQ=255) aCGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTg > 6:910227/1‑129 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaaga < 7:972495/111‑1 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGaagaag > 6:165962/1‑113 (MQ=255) cGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGaagaag < 5:165962/113‑1 (MQ=255) ggATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGaa < 2:43090/275‑1 (MQ=255) gATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATc < 2:503201/222‑1 (MQ=255) gACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATgaagaa > 6:178990/1‑103 (MQ=255) tATGGCAGGGAAGTTTCGCTGGATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATAttg < 2:443006/275‑1 (MQ=255) gggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAgg < 5:935020/95‑1 (MQ=255) gggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTG‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGCAGCCACTCTGCAACCAGATGGGGTGCGCGACATAAAAGAAAATATTAATTTTCATGCGCGAAATCAGCAGATTAAGCAAGGNTTTTATCGTGATTTACCACGACTAAGGATGCAGCCTGAATGGGTCGCTCGACTGTTCAACTACCATCTTGTTTGCGt > 3:122815/1‑275 (MQ=255) gggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCGCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAgg > 6:935020/1‑95 (MQ=255) gAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACGGCTGACCTATCATGTTGTTGGCggt > 2:486847/1‑272 (MQ=255) tttCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTCAT‑AAGAAGGGa < 6:631831/91‑1 (MQ=39) tttCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑CATAAGAAGGGa > 5:631831/1‑91 (MQ=255) tgcGCTGCCTTTTGCTTTTTATAGCAGGGCTGTTTGTATCAACTCTAAGTATTGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGGTCTCGCTCTTTGATGTTGAAGCCACGCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGt < 4:486846/273‑1 (MQ=255) ttttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGACACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTGTCATATTGTTGGCGTCACCCGTGATGGt < 2:106903/272‑1 (MQ=255) aTAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa > 8:492996/1‑90 (MQ=255) aTAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGaa < 7:492996/90‑1 (MQ=255) aGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACTGAGATCCCTTCTT‑ATGAAGTAGGGATCTCGCTCTTTGATGTTGACGCCACTCTGCAACCAGATGGGTTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGCGATTTACCACGACTATTGATGCAGCCTCATGGGGACGCTGCActgc > 2:511557/1‑224 (MQ=255) gCAGGTCTTTTTGTATCATCTCTAATNNTTGCAGAAAACACGGAGATCCCTTCTT‑ATGNAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCNTTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTg < 3:56980/272‑1 (MQ=255) cAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGa > 4:25877/1‑211 (MQ=255) cAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGa < 2:25878/211‑1 (MQ=255) ggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGTCGCGAAATCAGCCGCTTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCCGCCTGATGGAGAAGCATCACTGCTGAACTATCATATTTTTGGCATTACCCGTGATGGTATCTCTGAACCTCGGCaattt > 4:16623/1‑271 (MQ=255) ggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAAt > 1:63052/1‑146 (MQ=255) ggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAAc > 1:56980/1‑264 (MQ=255) cTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATg > 4:182118/1‑192 (MQ=255) tGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCActgc > 4:231252/1‑216 (MQ=255) tttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAACACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTTAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGACGCCTGGCATCTTGACTg > 2:277334/1‑275 (MQ=255) ttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGc < 2:182119/192‑1 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGa < 5:675447/114‑1 (MQ=255) tGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGa > 6:675447/1‑114 (MQ=255) ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTTCTGAACTATCATATTGTTGGCGTCCCCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCgg > 1:96795/1‑274 (MQ=255) tAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg < 5:335930/85‑1 (MQ=255) aGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGAGGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAAt < 1:132263/275‑1 (MQ=255) tGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCa > 4:150787/1‑218 (MQ=255) tGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCTGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATCCAGGGTTACTGattc > 4:73757/1‑272 (MQ=255) tGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGCCTGGCATATCGGTTTAATGTGTa > 4:70831/1‑275 (MQ=255) aaaCACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTa > 7:121752/1‑148 (MQ=255) cGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCt < 6:1101765/73‑1 (MQ=255) cGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCt > 5:1101765/1‑73 (MQ=255) gagaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag > 5:264783/1‑111 (MQ=255) gagaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag < 6:264783/111‑1 (MQ=255) gaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGAc < 6:750058/73‑1 (MQ=255) gaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGAc > 5:750058/1‑73 (MQ=255) gaTCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTa < 6:907657/148‑1 (MQ=255) tCCCTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATg < 7:182333/148‑1 (MQ=255) tCCCTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATg > 8:182333/1‑148 (MQ=255) cccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAAt < 6:1084540/103‑1 (MQ=255) cccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAAt > 5:1084540/1‑103 (MQ=255) cccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCaa < 7:550614/75‑1 (MQ=255) cccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCaa > 8:550614/1‑75 (MQ=255) ccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCtttt < 8:399829/124‑1 (MQ=255) ccTTCTT‑CATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCtttt > 7:399829/1‑124 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATca > 7:612690/1‑104 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATca < 8:612690/104‑1 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTg > 5:86277/1‑130 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTg < 6:86277/130‑1 (MQ=255) ccTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATgg < 6:547834/147‑1 (MQ=255) ctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCCGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTc > 8:375387/1‑87 (MQ=255) ctt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCCGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTc < 7:375387/87‑1 (MQ=255) tt‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAg < 8:121752/148‑1 (MQ=255) | AAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTT‑ATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTA > NC_000913/1324579‑1325085 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |