| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,832,194 | A→T | 5.6% | intergenic (+28/+25) | yicH → / ← yicI | putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,832,194 | 0 | A | T | 5.6% | 1529.5 / 52.4 | 435 | intergenic (+28/+25) | yicH/yicI | putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (200/205); new base T (12/13); total (215/220) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-02 | |||||||||||
Only 100 of 456 total aligned reads displayed.
CCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAG > NC_000913/3831923‑3832468 | ggaccgggTGTGCCGGGGAATAGTTTGCAACAGGGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGc < 4:429904/269‑1 (MQ=255) tgtgCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAt < 2:20315/274‑1 (MQ=255) ccGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAAGGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt < 2:372783/274‑1 (MQ=255) gAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTa < 4:394750/275‑1 (MQ=255) aaTATTTTGGAACGGTGGGGAGGGCCTGAATTACCGTGTACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCAGGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTa < 4:247950/274‑1 (MQ=255) attttttTCAACTGTGGGCCTGGCTGTANTNCCCGTTGACTGGCTCCGGCAATATTCCGCTTACCGCCAGTGGCGANTTTCAGTCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGNAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACa < 3:44311/272‑1 (MQ=255) aCAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGc < 2:522400/246‑1 (MQ=255) tGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTTAAACCTACGGTTAGCGGGCAATTTCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCTACGGAGCCGGTGCGGTAAACATCCAT‑CTCGCCGGGTACGACTCAGACCTACCCGGCATCCCATCAATAGATTACAACGTAATTTTCAg > 2:27726/1‑274 (MQ=255) tGGGGAGGGCCTGAATTCCCGTTGCCTGGGGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgc < 4:80037/275‑1 (MQ=255) gggATGGGCTGAATTACCGGTAGCTGGGGGCGGCAAAAGTCGGCTTACCGCGAGTGGGGATATAAAGGCCAAGGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCAGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcgc < 4:339242/275‑1 (MQ=255) gggATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcgc > 2:97714/1‑275 (MQ=255) gggATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATCCAATCAATAGATTAAAACGTAATTGTCAgcga > 1:144141/1‑274 (MQ=255) cTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATAACAACGTAATTGTCAGCGCATTCCCtt > 2:112768/1‑275 (MQ=255) cTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTAGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTCCAACGTAATTGTCAACGCCTTCCCt > 4:150088/1‑274 (MQ=255) cTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGGGCGGTAAAAATCAAT‑CTAGCCGGGTACGACTCAGACGTACCCGGCCTTCCATCAATAGATTACAACGTAATTGTAAGGGCATTCCCt > 4:527856/1‑274 (MQ=255) gAATTACCGTTGACTGGCAACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGa < 2:478014/275‑1 (MQ=255) ccGTTGACTGGGGACGGAAATATTCAGCTTACCGGCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCAGGCCGTGAACGCCGCAAAGCGGCAAGAGACTCAGACCATGAATGCTGGCATCAATTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt < 4:102989/274‑1 (MQ=255) ccGTTAACTGGCGCCGGCAAGATGCAGTTTACCGCCAGTGGCGATATTCAGGCCAAGGTCCCGTTGAACCCTACGGTTGGCGGGCAATTGAATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt < 2:29762/274‑1 (MQ=255) cGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTGCCGTTTAAACCTACGGTTAGCGGGCGATTTCATGCCGTGAACGCCGCAAAGCAGCAAGTGACCCAAACCATTAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAACCGGTGCAGCAAACTTCAAT‑CCCGCCGGGTACGACTCAAATGTACCCGGCATTCCACTACAAACGTAGAACGTAATTGTCAGCTCATTCCCTTGAGGCTTCACCACCAGcca > 4:484383/1‑274 (MQ=255) aaaTATTCAGCTTACGGCCAGTGGCGATATTAAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt < 2:503421/273‑1 (MQ=255) gCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCACCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCACCTGCTCACTTTCAGc > 1:115038/1‑275 (MQ=255) aCCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATTAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCCACACCAGCCCCTTCTTACTTTCAGCCTGc > 2:417347/1‑275 (MQ=255) cGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg < 2:262125/274‑1 (MQ=255) cGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCg > 1:7830/1‑274 (MQ=255) ccAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc < 4:256040/247‑1 (MQ=255) gCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATGCCATCAATAGCTTACAACGTAATTGTCAGTGCATTCCCTTTAGGCTTCACCCCCCGCCCCTGCCACCTTTCAGCCCTCCAACCGCTTTTCAAACCAtt > 4:413214/1‑274 (MQ=255) tGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACttt > 2:43053/1‑275 (MQ=255) cGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGCCCATTTACTTTCacaa > 2:332986/1‑275 (MQ=255) ttGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTCCCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTTAGGCTTCACCACCAGCCCCGGCTCACTTTCTGCCTCCGACCCGTCTTGCACACCCTTTACTTTCccagca > 1:20434/1‑275 (MQ=255) tACGGTTAGCGGGAAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAg < 2:150089/275‑1 (MQ=255) aCGGTTAGGGTGCAATTGCATGCCGTGAACTCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGGTTCCAGCTGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAg < 2:461449/274‑1 (MQ=255) ttAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcaca < 2:237714/275‑1 (MQ=255) aGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAACAATAGATTACAACGTAATTGCCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCCCACTTTCAGCCTGCCAACCGTCTTGCAGACCATTTACTTTCACAACATTCCGCAgtccact > 4:503219/1‑268 (MQ=255) gCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTa < 1:102307/158‑1 (MQ=255) gCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCa < 4:304340/275‑1 (MQ=255) aTTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGa > 7:851750/1‑128 (MQ=255) tGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGa > 7:501598/1‑126 (MQ=255) tGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTAcc < 6:98369/128‑1 (MQ=255) aaCGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACCGTGTCCAGTTCTTCGCCTCGccc > 2:7471/1‑275 (MQ=255) aaaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGt < 2:187603/275‑1 (MQ=255) aaaGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGt < 2:212461/275‑1 (MQ=255) tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGa < 5:395581/96‑1 (MQ=255) tGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGa > 6:395581/1‑96 (MQ=255) aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCATCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCgtg < 4:194746/271‑1 (MQ=255) ccATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGa > 6:845420/1‑87 (MQ=255) cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGTCTGAGTCGTAccc < 7:958925/93‑1 (MQ=255) cATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTAccc < 7:306319/93‑1 (MQ=255) tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGTCTGAGTCGTAcc < 5:691746/90‑1 (MQ=255) tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg < 6:285705/107‑1 (MQ=255) tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg > 5:285705/1‑107 (MQ=255) tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGa < 8:541336/80‑1 (MQ=255) tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc > 8:812017/1‑130 (MQ=255) gctgtctgcGTTGCCAGCGGGGAAGTTACATCACCGCAGCCGGTGCGGTAAACATACAT‑CTGGCCGGGTACGACTCAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTGTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGtgc < 4:176871/266‑1 (MQ=255) ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAt > 2:164437/1‑101 (MQ=255) ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt < 6:1063365/115‑1 (MQ=255) ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt > 5:1063365/1‑115 (MQ=255) ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGccc > 5:1092578/1‑148 (MQ=255) cATCGTTTCCAGCGGTGAATTTACATCTACGGGGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCttt < 1:56112/275‑1 (MQ=255) tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCa < 5:131384/88‑1 (MQ=255) tCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGcaca < 4:469376/209‑1 (MQ=255) tttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa < 6:1092578/148‑1 (MQ=255) ttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTa < 6:733202/72‑1 (MQ=255) cAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTtaaagtaaa < 2:124923/275‑1 (MQ=255) gCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCa < 2:390502/226‑1 (MQ=255) taaattaccgcGAGGGAGACGGGGCGGTAAAACTCAATGCTC‑CGAGGTACGAACCAGACGTACCCGGCATTCCCTCAATAGATTACCACGTAATTGTCAGCGCAGTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGAACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCAcc < 4:531527/265‑1 (MQ=255) aaGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGAAGTACCCGGCATTCCATCGATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCTCTTTCAGCCTGc < 8:987248/148‑1 (MQ=255) tACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc > 8:334162/1‑137 (MQ=255) tCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTcc > 6:602185/1‑99 (MQ=255) aCGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg < 6:450306/84‑1 (MQ=255) aCGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg > 5:450306/1‑84 (MQ=255) aCGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCAAGTTCTTCGCTTCGCCCGCACCAGTCAAAGTAATCGTGTTGCCAGTACGTGATGCTTTTAAAGTAAAGATCACCGATCCGTcagca > 2:34989/1‑275 (MQ=255) ggAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTTAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCAGTAagccgc > 1:64677/1‑275 (MQ=255) aGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCa < 4:562310/146‑1 (MQ=255) gCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGt < 6:127111/139‑1 (MQ=255) ccGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGc < 2:536304/275‑1 (MQ=255) gTATACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTCCGCAGGAAAAGTGTCCAGTTCTTCTCCTCGCCCGCACCAGTCCCAGTAATCGTGTTGCCAGGCAGTGCAGCTTTTACAGTAAAGATCATCTTTCCTCCAGCCACGGGCACTTcac > 4:326082/1‑274 (MQ=255) aaCATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca > 8:122079/1‑91 (MQ=255) aCATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCATGa > 8:677809/1‑83 (MQ=255) tCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTa < 5:876359/138‑1 (MQ=255) cAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCCCCGTAATCGTGTTTCCAGTACGTGCTGCTTTTAAAGTAAAGCTCACCGAACCGCCAGCCGCGGGCCCTCCACAGACGGCt > 4:380735/1‑274 (MQ=255) t‑ctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACAt > 2:561806/1‑146 (MQ=255) gggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGTTTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGa < 8:766584/120‑1 (MQ=255) ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTg > 7:64969/1‑67 (MQ=37) ggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAg > 8:410726/1‑118 (MQ=255) tACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTc > 7:1064701/1‑51 (MQ=255) tACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTc < 8:1064701/51‑1 (MQ=255) tCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAAc > 7:597579/1‑100 (MQ=255) tCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGAcc < 7:731237/113‑1 (MQ=255) tCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAgtgt > 5:826475/1‑146 (MQ=255) cAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTc > 7:994209/1‑53 (MQ=255) cAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTcaccac < 8:64170/70‑1 (MQ=255) cAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa < 8:272534/89‑1 (MQ=255) cAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTa < 8:852228/117‑1 (MQ=255) cAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCa < 7:794217/148‑1 (MQ=255) cAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCa > 5:560369/1‑148 (MQ=255) aGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCCGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTCAAGTAAAGATCACCGATCCGTCAGCATCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTg > 2:486224/1‑274 (MQ=255) gACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTgcgc > 5:158229/1‑132 (MQ=255) gACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAgtgt > 7:170990/1‑143 (MQ=255) gACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCa < 7:392849/146‑1 (MQ=255) aCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGccc < 6:164911/73‑1 (MQ=255) | CCTGAATGGACGCGGTGTGCCGGTGAATATTTTGCAACAGTGGGGATGGCCTGAATTACCGTTGACTGGCGACGGCAATATTCAGCTTACCGCCAGTGGCGATATTCAGGCCAATGTCCCGTTGAAACCTACGGTTAGCGGGCAATTGCATGCCGTGAACGCCGCAAAGCAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAAT‑CTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAACCGTCTTGCAGACCATTTACTTTCACAACATTGCGCAGGCACAGTGTCCAGTTCTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAG > NC_000913/3831923‑3832468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |