| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,853,603 | A→G | 6.2% | A17A (GCA→GCG) | tisB → | toxic membrane persister formation peptide, LexA‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,853,603 | 0 | A | G | 6.2% | 599.0 / 20.7 | 186 | A17A (GCA→GCG) | tisB | toxic membrane persister formation peptide, LexA‑regulated |
| Reads supporting (aligned to +/- strand): ref base A (82/91); new base G (5/7); total (88/98) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01 | |||||||||||
Only 100 of 194 total aligned reads displayed.
TCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGG > NC_000913/3853332‑3853873 | tCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGCCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTTGGTATCGCCATTCTTATCCCCAAACTCATTGTTCCAGCACt > 4:469298/1‑274 (MQ=255) tACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCCCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCAAAGGAGAAGCGTATGAACCT‑GGTGGATAACGCCATTCTTATCCTCAAACTCATTGTTGCAGACCTTCAACTg > 4:434242/1‑275 (MQ=255) tgtgTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACCGGCAGCGGCAAGGTACGTCCGCTGGCCATTCCCCTGAACCACAGGCACCGCATATGACCCTGGGTGATTATC‑CCATTCTTACCCTCAAACTCATTGTTGAAACACCGCCACTTCTTggt > 4:6865/1‑272 (MQ=255) tgtgTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATg < 4:334687/275‑1 (MQ=255) gtggtgaTGCGGGGGCCGCATCGGTACCCGGCGCACTAAGTCCTGTGTGAACCGGGTGGGGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATg < 4:485872/269‑1 (MQ=255) gggCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAAt > 2:449739/1‑275 (MQ=255) cgcACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAATTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGAtt > 1:123172/1‑275 (MQ=255) cTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACCGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCAATTCTTATCCCAAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTTAAATACCTGAAGTAATTCACATTCAAGTCGTCCCaaa > 4:530958/1‑274 (MQ=255) cTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTc > 4:173913/1‑261 (MQ=255) ccGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCg > 2:341843/1‑275 (MQ=255) tCAGCGCCTTAACCCCGCTTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTc < 2:202899/275‑1 (MQ=255) aGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTTCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTccc < 1:72617/275‑1 (MQ=255) cgTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCt > 2:215110/1‑210 (MQ=255) cgTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGACATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGACTCAAGTCGCACCAAAGGGGAGCGGGAAACCGATCCCCTTTTATATTTAGCg > 2:514641/1‑275 (MQ=255) acacTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGt < 4:341842/275‑1 (MQ=255) acaaGCACAGCGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACCCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGgatgcgat > 4:120326/1‑275 (MQ=255) cccaACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGAAACAGGCAGCGGAAAGGTACGTCACCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGa < 2:485977/273‑1 (MQ=255) tcCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTg < 6:183385/148‑1 (MQ=255) ttGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCtt > 8:1048993/1‑148 (MQ=255) ttGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCtt > 7:752478/1‑148 (MQ=255) ttGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCCCAAACTCATTGTTGCAGCACTGCa < 6:1019729/141‑1 (MQ=255) tGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTg < 6:77958/148‑1 (MQ=255) tACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGa < 6:137894/144‑1 (MQ=255) cgcgTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGtt > 8:241473/1‑148 (MQ=255) cgcgTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCAtc > 2:481462/1‑275 (MQ=255) gcgTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTc < 6:948558/148‑1 (MQ=255) gcgTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTc < 7:247774/148‑1 (MQ=255) gCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGaa < 5:951016/148‑1 (MQ=255) cacaACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTCGCAGCACTGCAACTGCTTGATGCTGTTc > 8:591770/1‑143 (MQ=255) caacaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGt > 8:633982/1‑139 (MQ=255) caacaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGaa < 6:247217/145‑1 (MQ=255) caacaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGaa > 5:247217/1‑145 (MQ=255) aacaGCTGGCAACAGGCAGCGGAAATGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTg > 5:1013585/1‑131 (MQ=255) aacaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGACCCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGCTGCTGACGCATCTTATCCGCCCTTACATCTCTccctg > 1:113985/1‑272 (MQ=255) aacaGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTC‑TATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc < 7:87175/148‑1 (MQ=255) caGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATa < 7:1071375/145‑1 (MQ=255) gCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTa > 2:62666/1‑275 (MQ=255) aCAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAt < 8:845500/122‑1 (MQ=255) aCAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGAt > 7:845500/1‑122 (MQ=255) cAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTc < 8:1008035/148‑1 (MQ=255) cgggtgcaaGGTACGTCAGATGGCATTGGTCCTGCGCCCCCGGCGACGCGTATGAACCG‑GGGGGTTTTCGCCTTTTTNTTCCTCCAGTTCTTTGTTGCAGCGTTGCAACTGCTTGAAGCTGTTCTCAAAGACTTGAAATCATTCAGATTCAAGTCGCTCCAAATGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCTGGTTGTTGGCGGATGGGATGCTGACGCATCTTATCCGCCGTACCATCTCTCCCGGCAACAGTTATTGCCGCt < 3:113985/268‑1 (MQ=255) cAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCAAcaa > 5:936336/1‑109 (MQ=255) cAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCAAcaa > 5:791543/1‑109 (MQ=255) cGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCa < 8:176351/148‑1 (MQ=255) cGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAAcc > 4:350806/1‑175 (MQ=255) gAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTg > 7:606488/1‑106 (MQ=255) aaaGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCAAca < 5:570895/104‑2 (MQ=255) aaaGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGa < 7:939242/130‑1 (MQ=255) aaaGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTg < 2:158239/195‑1 (MQ=255) aaGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTc < 2:350807/175‑1 (MQ=255) tACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTaa > 5:586536/1‑130 (MQ=255) tCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGaa < 6:520372/113‑1 (MQ=255) cAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTCTCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAg < 5:216523/148‑1 (MQ=255) cAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAg > 6:216523/1‑148 (MQ=255) tGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCa > 6:932560/1‑87 (MQ=255) tGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGTGCTGCa < 5:932560/87‑1 (MQ=255) gTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTc > 6:835019/1‑119 (MQ=255) cTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAc < 7:690359/104‑1 (MQ=255) tCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAATTACTTCAggta < 7:588341/120‑4 (MQ=255) ccTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAAt < 5:1035303/112‑1 (MQ=255) ccTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaaa < 7:1057299/146‑1 (MQ=255) cTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATg < 7:53660/87‑1 (MQ=255) cTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATg > 8:53660/1‑87 (MQ=255) cTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCaaa < 8:1057775/132‑1 (MQ=255) aaCCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTccc < 2:335783/274‑1 (MQ=255) acaGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGaaa > 4:104607/1‑91 (MQ=255) acaGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc > 5:921547/1‑95 (MQ=255) acaGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc < 6:921547/95‑1 (MQ=255) acaGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTa > 8:718090/1‑102 (MQ=255) gagaCGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATAcc < 2:104608/91‑1 (MQ=255) cgcgTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTaa > 6:498663/1‑95 (MQ=255) gtcgtaaccgtggggATATCTCCATTTTTATCCTCAAACTACTGGTTGCAGCACTGGAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGAAGCAAGTCGCACCAAATGGGAGCGGGAAACCGCTCCCTTTGTATATTTATCGTGCGGGTTGGTTTCGGATGCGATGCTGATGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTatat < 4:514640/262‑1 (MQ=255) aTGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTg > 8:215043/1‑60 (MQ=255) aTGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGaa < 5:511958/77‑1 (MQ=255) aTGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGa > 5:484220/1‑85 (MQ=255) aTGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGc < 5:1108677/106‑1 (MQ=255) aTGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCaa < 7:228972/111‑1 (MQ=255) aaCCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaaa < 5:225271/122‑1 (MQ=255) aaCCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGaaa > 6:225271/1‑122 (MQ=255) ccT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAgg < 5:140715/109‑1 (MQ=255) t‑ggtggATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAg > 6:1094208/1‑88 (MQ=255) ggtggATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTg < 7:174064/56‑1 (MQ=255) gATATCGCCATTCTTATCCTCAAACTCATTGTTgcagca < 4:362530/39‑1 (MQ=255) gATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCGCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAgg > 6:363591/1‑102 (MQ=255) gATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCGCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAgg < 5:363591/102‑1 (MQ=255) atatCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAg > 6:63982/1‑100 (MQ=255) tatCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCa < 3:69893/183‑1 (MQ=255) tCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAAATGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGAGGGAAAc > 6:576780/1‑110 (MQ=255) cGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAgg < 8:1096250/97‑1 (MQ=255) aTCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAg > 6:767855/1‑86 (MQ=255) aTCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGAtt > 4:498928/1‑275 (MQ=255) cAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCaa > 6:450697/1‑79 (MQ=255) cAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCaa < 5:450697/79‑1 (MQ=255) cAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGc < 8:88942/97‑1 (MQ=255) aaaCTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTCACATTCATCAATGTGATAGATTCCtctc > 2:426240/1‑275 (MQ=255) attCACTGTTGAAGCACGTCAACTGCTTGAGGCTGTACTGAAATACCTGAAGTAATGTAGCTTCAAGTCGCACCAGAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTGGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGATAAACAATTCCCCATGCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTccc < 4:13099/273‑1 (MQ=255) cagcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATggg < 2:120327/275‑1 (MQ=255) gcaCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAgg > 5:76313/1‑66 (MQ=255) caCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAg < 7:204648/69‑1 (MQ=255) aCTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCg > 7:1041858/1‑70 (MQ=255) | TCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCGTCAGCGCCTTAACCCCGCGTGAGCACACTGTGTTATGTCAACAAGCACAACGTTTCTCCTTGAGATACCGCGTGCACAACAGCTGGCAACAGGCAGCGGAAAGGTACGTCAGCTGGCAGTGCTCCTGAACCACAGGAGACGCGTATGAACCT‑GGTGGATATCGCCATTCTTATCCTCAAACTCATTGTTGCAGCACTGCAACTGCTTGATGCTGTTCTGAAATACCTGAAGTAATTCAGATTCAAGTCGCACCAAAGGGGAGCGGGAAACCGCTCCCCTTTTATATTTAGCGTGCGGGTTGGTGTCGGATGCGATGCTGACGCATCTTATCCGCCCTACCATCTCTCCCGGCAACATTTATTGCCGCTTTTGTTTACATATTCTGCCGCTAAACAATTCCCCATTCCTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGG > NC_000913/3853332‑3853873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |