| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,322,146 | Δ1 bp | 5.9% | coding (281/1386 nt) | atoC → | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,322,146 | 0 | C | . | 5.9% | 244.8 / 8.9 | 68 | coding (281/1386 nt) | atoC | fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein |
| Reads supporting (aligned to +/- strand): ref base C (33/31); new base . (2/2); total (35/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
TGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCA > NC_000913/2322017‑2322263 | tggtgTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑Ctt > 2:485326/1‑148 (MQ=255) tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGc > 4:882075/1‑131 (MQ=255) tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGc < 3:882075/131‑1 (MQ=255) tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGc < 3:282964/139‑1 (MQ=255) tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGc > 4:282964/1‑139 (MQ=255) gCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg > 3:71872/1‑132 (MQ=255) gCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg < 4:71872/132‑1 (MQ=255) agagATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGAt < 1:485326/148‑1 (MQ=255) gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt < 1:122067/142‑1 (MQ=255) gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt > 2:122067/1‑142 (MQ=255) aCGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa > 1:422085/1‑145 (MQ=255) aCGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa < 2:422085/145‑1 (MQ=255) aTCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAg < 1:181876/144‑1 (MQ=255) aTCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAg > 2:181876/1‑144 (MQ=255) tCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg > 2:801969/1‑111 (MQ=255) tCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCg < 1:801969/111‑1 (MQ=255) aCTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa > 2:739549/1‑132 (MQ=255) aCTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGa < 1:739549/132‑1 (MQ=255) cTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGttatta > 3:828110/1‑115 (MQ=255) cTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGttatta < 4:828110/115‑1 (MQ=255) aaaGGAGATGCGCAGCCATGAGATCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTGCGACTATGTTATTAAACCGTGTGATGTCGATGAGTTGAAGTTAATCGtt > 3:964028/1‑148 (MQ=255) aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg < 1:490153/91‑1 (MQ=255) aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg > 2:490153/1‑91 (MQ=255) aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 2:600870/142‑1 (MQ=255) aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 1:600870/1‑142 (MQ=255) aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAG‑GCTCCGCAGCGTAGcgctt > 3:432615/1‑93 (MQ=38) ggAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 1:367494/141‑1 (MQ=255) ggAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 2:367494/1‑141 (MQ=255) agaTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAgcg < 1:701789/77‑1 (MQ=255) agaTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAgcg > 2:701789/1‑77 (MQ=255) aTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 1:5671/1‑137 (MQ=255) aTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 2:5671/137‑1 (MQ=255) gcAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTa < 2:817263/131‑1 (MQ=255) gcAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTa > 1:817263/1‑131 (MQ=255) agaCCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt < 3:799178/99‑1 (MQ=255) agaCCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGt > 4:799178/1‑99 (MQ=255) cccGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt > 1:920666/1‑121 (MQ=255) cccGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt < 2:920666/121‑1 (MQ=255) cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGtt < 1:576440/76‑1 (MQ=255) cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGtt > 2:576440/1‑76 (MQ=255) cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATg < 3:779514/98‑1 (MQ=255) cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATg > 4:779514/1‑98 (MQ=255) cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt > 1:927249/1‑126 (MQ=255) cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt < 2:927249/126‑1 (MQ=255) ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa > 1:634624/1‑137 (MQ=255) ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa < 2:660128/137‑1 (MQ=255) ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa > 1:660128/1‑137 (MQ=255) ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa < 2:634624/137‑1 (MQ=255) tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTaaa < 1:591784/76‑1 (MQ=255) tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTaaa > 2:591784/1‑76 (MQ=255) tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGaa > 4:787575/1‑99 (MQ=255) tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGaa < 3:787575/99‑1 (MQ=255) gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAAt > 4:175852/1‑97 (MQ=255) gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAAt < 3:175852/97‑1 (MQ=255) gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAgcgcg > 1:1097583/1‑113 (MQ=255) gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAgcgcg < 2:1097583/113‑1 (MQ=255) tgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg > 3:794789/1‑49 (MQ=255) tgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg < 4:794789/49‑1 (MQ=255) aCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑Ctt < 3:225817/53‑1 (MQ=255) aCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑Ctt > 4:225817/1‑53 (MQ=255) ccTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCa > 2:1001795/1‑148 (MQ=255) aaGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc < 1:445250/90‑1 (MQ=255) aaGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc > 2:445250/1‑90 (MQ=255) aaCCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt > 1:564908/1‑93 (MQ=255) aaCCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt < 2:564908/93‑1 (MQ=255) catagTCGAAG‑GCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa > 4:254321/5‑93 (MQ=21) catagTCGAAG‑GCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa < 3:901276/89‑1 (MQ=21) catagTCGAAG‑GCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa < 3:254321/89‑1 (MQ=21) cGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc < 2:322546/74‑1 (MQ=255) cGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc > 1:322546/1‑74 (MQ=255) cgcTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAag < 3:930602/102‑1 (MQ=255) cgcTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAag > 4:930602/1‑102 (MQ=255) | TGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGC‑CTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCA > NC_000913/2322017‑2322263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |