| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,017,216:1 | +A | 7.4% | intergenic (+24/‑117) | udp → / → rmuC | uridine phosphorylase/DNA recombination protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,017,216 | 1 | . | A | 7.4% | 230.2 / 13.7 | 54 | intergenic (+24/‑117) | udp/rmuC | uridine phosphorylase/DNA recombination protein |
| Reads supporting (aligned to +/- strand): ref base . (26/24); new base A (2/2); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
GGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAC > NC_000913/4017070‑4017356 | ggTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑a < 1:68697/148‑1 (MQ=255) ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCg < 1:259477/134‑1 (MQ=255) ttAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCg > 2:259477/1‑134 (MQ=255) tAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCg > 2:302079/1‑124 (MQ=255) tAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCg < 1:302079/124‑1 (MQ=255) agagATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTg < 1:571072/134‑1 (MQ=255) agagATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTg > 2:571072/1‑134 (MQ=255) agaTCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑Acgcg < 4:240693/112‑1 (MQ=255) agaTCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑Acgcg > 3:240693/1‑112 (MQ=255) aCGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTg > 3:69625/1‑148 (MQ=255) tGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGtt < 1:53987/93‑1 (MQ=255) tGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGtt > 2:53987/1‑93 (MQ=255) acaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGt > 1:884015/1‑88 (MQ=255) acaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGt < 2:884015/88‑1 (MQ=255) acaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCt < 4:663/117‑1 (MQ=255) acaaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCt > 3:663/1‑117 (MQ=255) caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTg < 3:179372/98‑1 (MQ=255) caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTg > 4:179372/1‑98 (MQ=255) caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGc < 3:619311/119‑1 (MQ=255) caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGc > 4:619311/1‑119 (MQ=255) caaacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGt < 4:69625/148‑1 (MQ=255) aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAtttt > 3:429829/1‑102 (MQ=255) aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAtttt < 4:429829/102‑1 (MQ=255) aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAt > 1:918566/1‑99 (MQ=255) aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTAt < 2:918566/99‑1 (MQ=255) aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTgg > 4:47378/1‑138 (MQ=255) aacCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTgg < 3:47378/138‑1 (MQ=255) ccGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGcc > 1:512415/1‑89 (MQ=255) ccGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGcc < 2:512415/89‑1 (MQ=255) ggtggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCG‑TCGGCCttc < 2:986685/67‑2 (MQ=37) ggtggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGAACGCG‑TCGGCCttc > 1:986685/1‑66 (MQ=37) tggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGaa < 4:694283/93‑1 (MQ=255) tggAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGaa > 3:694283/1‑93 (MQ=255) gTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgt < 1:473206/146‑1 (MQ=255) gTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgt > 2:473206/1‑146 (MQ=255) tctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGt < 4:127753/128‑1 (MQ=255) tctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGt > 3:127753/1‑128 (MQ=255) tctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCagg > 4:964797/1‑132 (MQ=255) tctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCagg < 3:964797/132‑1 (MQ=255) tctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtc > 3:386635/1‑148 (MQ=255) ctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtct > 3:584269/1‑148 (MQ=255) ctcttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTtct < 4:584269/148‑1 (MQ=255) cttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGTCTCGCAGGAAATGCCTTTCCaa > 4:785309/1‑75 (MQ=255) cttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCaa < 3:785309/75‑1 (MQ=255) cttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGtt > 3:110528/1‑84 (MQ=255) cttctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGtt < 4:110528/84‑1 (MQ=255) tctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtg > 1:387054/1‑104 (MQ=255) tctcCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtg < 2:387054/104‑1 (MQ=255) tGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgt < 1:1129207/113‑1 (MQ=255) tGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAgttgt > 2:1129207/1‑113 (MQ=255) gTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGa < 2:367151/54‑1 (MQ=255) gTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGa > 1:367151/1‑54 (MQ=255) gAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAc > 3:18171/1‑148 (MQ=255) aaGGCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGt < 2:945181/100‑1 (MQ=255) aaGGCCGAACGCG‑TCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGt > 1:945181/1‑100 (MQ=255) ccG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg > 2:148088/1‑123 (MQ=255) ccG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTgg < 1:148088/123‑1 (MQ=255) g‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTc < 4:322241/53‑1 (MQ=255) g‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTc > 3:322241/1‑53 (MQ=255) g‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtgt > 3:721330/1‑88 (MQ=255) g‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTtgt < 4:721330/88‑1 (MQ=255) | GGTATGGTAGCGGGTGTTATCGTTAACCGCACCCAGCAAGAGATCCCGAATGCTGAGACGATGAAACAAACCGAAAGCCATGCGGTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCG‑ACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTAC > NC_000913/4017070‑4017356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |