| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,066,310 | A→T | 5.6% | H288Q (CAT→CAA) | ygfI ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,066,310 | 0 | A | T | 5.6% | 261.1 / 3.8 | 71 | H288Q (CAT→CAA) | ygfI | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (32/35); new base T (2/2); total (34/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAA > NC_000913/3066167‑3066456 | gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCt < 1:971943/148‑1 (MQ=255) ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCATAT‑TGCTGa < 2:732539/148‑1 (MQ=255) aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAg < 1:6167/148‑1 (MQ=255) cTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCt < 4:455627/142‑1 (MQ=255) cTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCt > 3:455627/1‑142 (MQ=255) gCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATAT‑TGCTGAAgatc > 4:113502/1‑145 (MQ=255) cGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGCCACGATGCTTCAGCATAT‑TGCTGAAG‑ATCGTga < 3:113502/148‑2 (MQ=38) ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCAt < 4:287493/135‑1 (MQ=255) ggAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCAt > 3:287493/1‑135 (MQ=255) aaaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGa > 1:448205/1‑128 (MQ=255) aaaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGa < 2:448205/128‑1 (MQ=255) aaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACga < 3:395241/148‑1 (MQ=255) aaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑Tg < 1:667763/122‑1 (MQ=255) aaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑Tg > 2:667763/1‑122 (MQ=255) gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCAt > 3:887255/1‑118 (MQ=255) gACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCAt < 4:887255/118‑1 (MQ=255) tCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACga > 1:771076/1‑127 (MQ=255) tCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACga < 2:771076/127‑1 (MQ=255) tCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTc < 2:54874/134‑1 (MQ=255) tCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTc > 1:54874/1‑134 (MQ=255) acGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGa > 4:620460/1‑136 (MQ=255) acGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGa < 3:620460/136‑1 (MQ=255) cTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGa < 2:395660/96‑1 (MQ=255) cTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGa > 1:395660/1‑96 (MQ=255) cACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa > 3:176458/1‑130 (MQ=255) cACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaaa < 4:176458/130‑1 (MQ=255) cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCTTGGCGGtata < 3:917557/105‑1 (MQ=255) cGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGtata > 4:917557/1‑105 (MQ=255) tCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATGCTTCAGCATAT‑TGCTGAAg > 1:12284/1‑86 (MQ=37) tCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATGCTTCAGC‑ATATTGCTGAAg < 2:12284/86‑1 (MQ=37) gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACgatgat > 4:73215/1‑96 (MQ=255) gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACgatgat < 3:73215/96‑1 (MQ=255) gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTg > 1:169100/1‑101 (MQ=255) gTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTg < 2:169100/101‑1 (MQ=255) aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa < 1:669309/108‑1 (MQ=255) aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa > 2:669309/1‑108 (MQ=255) tttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTcc > 4:588337/1‑115 (MQ=255) tttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTcc < 3:588337/115‑1 (MQ=255) taattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGaa < 4:752280/66‑1 (MQ=255) taattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGaa > 3:752280/1‑66 (MQ=255) aCCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGa > 3:1024673/1‑92 (MQ=255) aCCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGa < 4:1024673/92‑1 (MQ=255) gTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAata > 4:525080/1‑124 (MQ=255) gTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATAGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAata < 3:525080/124‑1 (MQ=255) ttAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑Tg > 3:682989/1‑36 (MQ=255) ttAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑Tg < 4:682989/36‑1 (MQ=255) gACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCa > 3:105336/1‑148 (MQ=255) cTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCg < 1:832377/103‑1 (MQ=255) cTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCg > 2:832377/1‑103 (MQ=255) tCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa > 4:529855/1‑84 (MQ=255) tCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa < 3:529855/84‑1 (MQ=255) tCGACCCGTCTAGAT‑CTACAGAATCA‑TGCTGAAGCATCCTGGCGGTATAACGGTCATTTCTGAAAAAATAAAGATTTTCGTGCTTCTGCAACCGCCAGTCCGGTGCATCAAAATATTTACACGTTGCCGCATCCTCCAGTCGCCAgtc < 4:105336/148‑3 (MQ=255) cGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTg > 3:1017789/1‑61 (MQ=255) cGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTg < 4:1017789/61‑1 (MQ=255) cGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAat < 3:73448/113‑1 (MQ=255) cGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAat > 4:73448/1‑113 (MQ=255) tCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa > 1:1100203/1‑68 (MQ=255) tCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATaa < 2:1100203/68‑1 (MQ=255) tCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTc > 4:1080497/1‑116 (MQ=255) tCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTc < 3:1080497/116‑1 (MQ=255) cACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGc > 2:1035263/1‑134 (MQ=255) cACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGc < 1:1035263/134‑1 (MQ=255) aT‑CTTCTGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACAt < 4:686539/78‑1 (MQ=255) aT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACAt > 3:686539/1‑78 (MQ=255) t‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCg < 4:1070842/88‑1 (MQ=255) t‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCg > 3:1070842/1‑88 (MQ=255) cTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCAc > 3:699156/1‑148 (MQ=255) gCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg < 3:973699/83‑1 (MQ=255) gCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCgg > 4:973699/1‑83 (MQ=255) aaTA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTc < 3:82941/145‑1 (MQ=255) aaTA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTc > 4:82941/1‑145 (MQ=255) ta‑tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaaa > 3:1090486/1‑148 (MQ=255) | GATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGAT‑CTTCAGCAATA‑TGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAA > NC_000913/3066167‑3066456 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |