| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,256,450 | C→T | 7.4% | V68I (GTT→ATT) | yhaM ← | putative L‑serine dehydratase alpha chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,256,450 | 0 | C | T | 7.4% | 219.4 / 5.9 | 67 | V68I (GTT→ATT) | yhaM | putative L‑serine dehydratase alpha chain |
| Reads supporting (aligned to +/- strand): ref base C (30/32); new base T (2/3); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
AGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTT > NC_000913/3256303‑3256593 | agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAAc > 1:724645/1‑148 (MQ=255) cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGt > 1:261396/1‑148 (MQ=255) tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTg < 2:261396/148‑1 (MQ=255) ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGa < 4:915827/148‑1 (MQ=255) cccGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGc < 1:302302/148‑1 (MQ=255) ccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGAcc > 4:966036/1‑148 (MQ=255) tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatc < 2:724645/148‑1 (MQ=255) ttGGCGTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatca > 2:801630/1‑148 (MQ=255) ttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGgaga > 3:517420/1‑127 (MQ=255) ttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGa < 4:517420/127‑1 (MQ=255) aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGa > 1:473056/1‑115 (MQ=255) aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGa < 2:473056/115‑1 (MQ=255) tGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAcc < 1:801630/148‑1 (MQ=255) tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg > 2:850591/1‑135 (MQ=255) tCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAg < 1:850591/135‑1 (MQ=255) cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCATATTTGGCGAAACCCAGGCTTCTACACGTTc > 3:932968/1‑148 (MQ=255) ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGAATgg > 2:203273/1‑91 (MQ=255) ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGAATgg < 1:203273/91‑1 (MQ=255) ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt < 1:670243/138‑1 (MQ=255) ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt > 2:670243/1‑138 (MQ=255) ttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCaa < 3:966036/148‑1 (MQ=255) cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc < 2:715380/106‑1 (MQ=255) cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 1:104118/1‑106 (MQ=255) cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc < 2:104118/106‑1 (MQ=255) cAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttc > 1:715380/1‑106 (MQ=255) gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatc < 1:815704/110‑1 (MQ=255) gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatc > 2:815704/1‑110 (MQ=255) gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCa < 2:473889/127‑1 (MQ=255) gCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCa > 1:473889/1‑127 (MQ=255) cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatc < 1:802579/107‑1 (MQ=255) cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTtcatc > 2:802579/1‑107 (MQ=255) cTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCGTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAcc < 4:932968/148‑1 (MQ=255) tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCTATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAcc < 3:714139/146‑1 (MQ=255) tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGAcc > 4:714139/1‑146 (MQ=255) cATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAtt > 2:1101750/1‑94 (MQ=255) cATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAtt < 1:1101750/94‑1 (MQ=255) tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCA‑CGGGAATGGTg > 2:770633/1‑66 (MQ=21) tttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCG‑GCACGGGAATGGTg < 1:770633/66‑1 (MQ=21) ttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttct < 3:1079140/81‑1 (MQ=255) ttCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGttct > 4:1079140/1‑81 (MQ=255) ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt < 3:1114774/112‑1 (MQ=255) ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGtt > 4:1114774/1‑112 (MQ=255) ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc < 3:160330/126‑1 (MQ=255) ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc > 4:160330/1‑126 (MQ=255) cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc < 1:1048163/126‑1 (MQ=255) cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGcc > 2:1048163/1‑126 (MQ=255) gccgccgcAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcca < 1:240922/145‑1 (MQ=255) gccgccgcAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcca > 2:240922/1‑145 (MQ=255) tCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAg > 3:434394/1‑58 (MQ=255) tCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAg < 4:434394/58‑1 (MQ=255) tCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCaa < 1:11421/90‑1 (MQ=255) tCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCaa > 2:11421/1‑90 (MQ=255) cAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccg > 3:6591/1‑116 (MQ=255) cAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccg < 4:6591/116‑1 (MQ=255) cAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc > 3:443280/1‑130 (MQ=255) cAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc < 4:443280/130‑1 (MQ=255) caccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt > 2:642859/1‑89 (MQ=255) caccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCtt < 1:642859/89‑1 (MQ=255) ccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc < 1:771795/89‑1 (MQ=255) ccaTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc > 2:771795/1‑89 (MQ=255) caTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAAt > 3:823785/1‑129 (MQ=255) caTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAAt < 4:823785/129‑1 (MQ=255) aTTCCCGTGC‑CGGGAACGGTGACGCCCAGGCCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTc < 1:531435/86‑1 (MQ=255) aTTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGGTCAACCGGACCTTc > 2:531435/1‑86 (MQ=255) tCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCg > 2:115594/1‑95 (MQ=255) tCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCg < 1:115594/95‑1 (MQ=255) ccggcacGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCgg < 1:648838/142‑1 (MQ=255) ggAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACtt > 4:823808/1‑148 (MQ=255) | AGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGC‑CGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTT > NC_000913/3256303‑3256593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |