BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,992,624	T→G	L491R (CTG→CGG)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,992,624	0	T	G	96.6%	506.4 / NA	148	L491R (CTG→CGG)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (74/67); total (78/70)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTT  >  NC_000913/3992354‑3992894

cgcgTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACgggtg                                                                                                                                                                                                                                                                              >  1:537302/1‑275 (MQ=255)

     cGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCT‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTTTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACGGTTACCTGAATAAACTGGAGGCGTg                                                                                                                                                                                                                                                                         >  1:8588/1‑275 (MQ=255)

       aaaTAACCTTAGCGTCCCCGCCAGTCCCCAGGATTTCCGCGTGCTGACGAGTCAGCGGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGTGTCTGACCTTTATTTGTGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTGTCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTggg                                                                                                                                                                                                                                                                       <  3:591649/274‑1 (MQ=255)

       aaaTAACCGAGGCGGCTCCGCCAGTCCGCAGGATAGCGGCGTGCTGACGCGTAAGCTGGATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCa                                                                                                                                                                                                                                                                     <  2:12616/276‑1 (MQ=255)

                 aGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTa                                                                                                                                                                                                                                                              >  1:733667/1‑274 (MQ=255)

                        ccGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGGCGATCATGAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATCAACGGGTGGCGTGGGCATGGTTTAACTGCctact                                                                                                                                                                                                                                                    >  2:740929/1‑277 (MQ=255)

                              gTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑Acc                                                                                                                                                                                                                                               <  1:113626/275‑1 (MQ=255)

                                  gCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCa                                                                                                                                                                                                                                        <  2:363421/278‑1 (MQ=255)

                                    aGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTcgcg                                                                                                                                                                                                                                          <  1:875438/274‑1 (MQ=255)

                                     ggttatCGGGGGGCTGAGCGGTAAGCTGTATGCCGGGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGGTCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCa                                                                                                                                                                                                                                        <  3:426520/272‑1 (MQ=255)

                                         atCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTAGATCATCAGCCATAAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGGTTAAGGGCCTGCTG‑ACCGCGCGCAC‑CCggtg                                                                                                                                                                                                                                >  2:301497/1‑275 (MQ=255)

                                         agcgggttGCTGACCCGTAAGCCGTATGGCGCGTTTTAAGCATTACCAAGTAAAAGTACGCGGGTAAACCCGCAGATTTCCCCCGAGCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCg                                                                                                                                                                                                                                   <  3:304848/268‑1 (MQ=255)

                                          tCGGCGGGCGGAGGTGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGGGACGCTGGTAAACCCGCAGATTTCACCCGATCCCTCGGAACCGAATCTAACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTTGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGt                                                                                                                                                                                                                                  <  3:141505/275‑1 (MQ=255)

                                           cGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGCACATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACCGGTGGCGGTGGCATGGTTTAACGGCCTGCTGAACCTcggcccccg                                                                                                                                                                                                                                     >  3:399666/1‑266 (MQ=255)

                                              cGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGt                                                                                                                                                                                                                              <  1:26532/275‑1 (MQ=255)

                                              cGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTAGGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTg                                                                                                                                                                                                                               <  1:399667/274‑1 (MQ=255)

                                                tGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGGTTGta                                                                                                                                                                                                                             >  1:345706/1‑274 (MQ=255)

                                                  cTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTT‑ACCTCGCCCAC‑CCGTCTGCatat                                                                                                                                                                                                                          >  3:850913/1‑275 (MQ=255)

                                                  cTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAc                                                                                                                                                                                                                                       >  3:250609/1‑263 (MQ=255)

                                                  cTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTa                                                                                                                                                                                                                        <  2:552657/277‑1 (MQ=255)

                                                      cgcgTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGt                                                                                                                                                                                                                                  <  1:250610/263‑1 (MQ=255)

                                                        cgTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCAGGGTTTACCGGCCTGCGG‑ACCTCGCGCAC‑CAGTTTGTATATTAAAgg                                                                                                                                                                                                                    >  1:5197/1‑275 (MQ=255)

                                                                        cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATCTTGTGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAATCGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑TCCTTGCGCAC‑CCGTTCGTATATTAAAGGTAACGGCTATGTCGTTTTg                                                                                                                                                                                                 >  2:193796/1‑278 (MQ=255)

                                                                        cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGCGTAGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAACAGGGTGGCGGGGGCAGGGTTTAACGGCCTTCTT‑ACCTCGC‑CACACCGTTTGTATATTAAAGGTAACGGCAATGTCACTTTg                                                                                                                                                                                                 >  4:797835/1‑278 (MQ=255)

                                                                        cgTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGGTTGTATATTAAAGGTAACGGCAATGTCGAt                                                                                                                                                                                                    >  1:372478/1‑275 (MQ=255)

                                                                          gttGAATCATTGACAGGTAAATTGACGCGTGTAAACGCGGTGGTTGCAGCCGGTCAGTTGGAACCGAAGCTGACCTGTGTTTCTGTGGCG‑CCGGGCCGGGCTAACCGTTCAGGGTGGTATCTGGATAAGCGCG‑CGCGAAATATTGAGTCGATCATAAGCCAGCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGcc                                                                                                                                                                                               <  4:739961/277‑1 (MQ=255)

                                                                               aGCGTTGCCAGGTAAAAGGACGCGGGTAAACCATCAGATTTCACCCGAGTTCTCGGAACCGAATCTGACCTGTATTTATGGGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCTTCAGCCGCTGGAATATAACCGTTACCTAAATAAACGGGTTGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTa                                                                                                                                                                                             <  3:754678/275‑1 (MQ=255)

                                                                                 cATTACCCGGTAAAGGGACCCTGGTAAAACCGACTATTTCACCCCATCTTTCGAAACCGAATCTGACCTTTTATTGTGTGCCG‑CCGGGCCTGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑AGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTg                                                                                                                                                                                        <  4:378308/278‑1 (MQ=255)

                                                                                    tACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTaa                                                                                                                                                                                            >  1:637804/1‑271 (MQ=255)

                                                                                    tACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTg                                                                                                                                                                                        <  3:637803/275‑1 (MQ=255)

                                                                                     aCCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑Acc                                                                                                                                                                                                                                               >  1:573742/1‑220 (MQ=255)

                                                                                     aCCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑Acc                                                                                                                                                                                                                                               <  3:573741/220‑1 (MQ=255)

                                                                                                    gCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCCGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTCATTTAAAGGTAACGGCCTTGTCGTTTTTCCTAAGTTGCAGGAGATGGTCGCCg                                                                                                                                                                        >  3:580369/1‑275 (MQ=255)

                                                                                                     cTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGGTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCATGAGATGGTCGCCTACgt                                                                                                                                                                    >  2:6516/1‑278 (MQ=255)

                                                                                                        gTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTcgcg                                                                                                                                                                                                                                          <  3:680510/206‑1 (MQ=255)

                                                                                                        gTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACg                                                                                                                                                                     >  1:587470/1‑274 (MQ=255)

                                                                                                           aaCCCGCGGATTTCCCCCGATCTTTCGGAACCGATTCTGACCTTTATTTCTTTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTc                                                                                                                                                                 <  3:224055/275‑1 (MQ=255)

                                                                                                            aCCCGCAGATTTCACCCTCTCTCTCGAAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCg                                                                                                                                                                <  3:372477/275‑1 (MQ=255)

                                                                                                                gCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑AACTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAATTGCCGGAGATGGTAGCCGACggttgtgcccatt                                                                                                                                                         >  4:725101/1‑266 (MQ=255)

                                                                                                                        cACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTTTATAACCGCG‑CGCCAAATATTGAGTCTATCATCAGCCATCAGCCGATGGAATATCACCGTTACATGCATAAACGGGTGGCGGTGGCATAGCTTAACGACCTGCTG‑ACCTCGCGCAC‑CCGGCTGTATTTTAAAGGTAACGGCATTGTTGATTTACCTTAGTTGCAGCAGATGGTTGCCGACCTGTctaacaactttccc                                                                                                                                                    >  3:259290/1‑262 (MQ=255)

                                                                                                                              aTCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTTTCGCACCATTTCCCGCTGCGCtt                                                                                                                                            >  2:752943/1‑277 (MQ=255)

                                                                                                                                      gAACCGAATCTGTCCTTTAGTTATTGGCCT‑CCGGGCCGGCCTAACCGTTCAGGTTGGTATCTTTATAACCGCG‑CGCCAAATATTTAGTCGATCATCAGCCATCTGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACTGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGcacc                                                                                                                                   <  2:244704/278‑1 (MQ=255)

                                                                                                                                            aaTCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTTGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGGACCATTTCCCGCTTGGCTTTCCTGcaccgacccc                                                                                                                             >  4:508314/1‑278 (MQ=255)

                                                                                                                                             aTCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCCTTTCCCGCTGCGCTTACCTGcaccgccacc                                                                                                                             >  2:722641/1‑277 (MQ=255)

                                                                                                                                             aTCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTAAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTg                                                                                                                                                                                                                               >  2:550451/1‑179 (MQ=255)

                                                                                                                                                tGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAg                                                                                                                         <  2:410788/278‑1 (MQ=255)

                                                                                                                                                  gcccttatttctGGGCCG‑GCGGGCCGGGCTAACCGTTCAGGGTGGTATGTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGgc                                                                                                                       <  4:332366/274‑1 (MQ=255)

                                                                                                                                                      tttatttcgtgCCGCCCGGGCCGGGCTCACCGTCTAGTTGGGTATCTGTATAACCGCG‑GGCCAAATAGTGGGGCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGgc                                                                                                                       <  3:5197/267‑1 (MQ=255)

                                                                                                                                                      ttcttcatGTGCCG‑CCGGGCCGGGCTACCCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCCGCCGCTGGAATATAACCGTTACCTGAATGAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTT‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGACATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGgcg                                                                                                                      <  1:805077/269‑1 (MQ=255)

                                                                                                                                                         ggttcaggccg‑ccGGGCCGGTAGAGCCGGTCAGAGTAGCACCTGTACAACCGCG‑GGCCAAATATTGAGACGATAATCAGCCAACAGCCGCTGGAATATCACCGTTGCCTGAATAGACGGGTGGCGTGGGCATTGTTTACGGGGATGCTG‑ACATCGCGCAC‑GCGTTTGTATAGTAAAGGTACCGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTa                                                                                                                 <  4:728067/270‑1 (MQ=255)

                                                                                                                                                           tacggcccg‑ccgGGCCGGGGGACACGGTCCGGTTGGGATCTGAATAACCAGG‑CGCCAAATATTGAGTCGATCAGCAGCCATCAGCCGCGGGAATAGAGCCGTTGCCTGAAGAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAg                                                                                                              <  4:294979/272‑1 (MQ=255)

                                                                                                                                                            atGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGGCGCCCCATTTCCCGTTGCGCTTACCTCCACCTACCCCCAAGGAGCTCTa                                                                                                                 >  3:604770/1‑274 (MQ=255)

                                                                                                                                                            atGTGCAG‑CCGGGCGGTGGTAACCGTTGAGGTTGG‑GTCCGTTAAAACGCGCCGGCCAATATTGAGTAGATCATCAGCGCACAGCCGCTGGAATATAACCGTGAGCTGAATAAACGGGTTGCGTGGGCATGGGTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGc                                                                                                             <  4:173065/278‑1 (MQ=255)

                                                                                                                                                              gtgCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCCTCAGCCATCAGCCGCTGGAATATAACCGTTACCTGCATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTTCCTAAGTTTCAGGTGCTGGGCGCCGACGTTTCGCCCCCTTTCCCCCTCCTCTTACCTTCCCCGCCCCCGCATGCCCTCCAct                                                                                                               >  3:661986/1‑273 (MQ=255)

                                                                                                                                                              gtgCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGCAGGCGCTCTGCAGcc                                                                                                            >  2:306386/1‑277 (MQ=255)

                                                                                                                                                              gtgCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGGTTGTATATTAAAGGTAACGGCATTGTCGTTTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGGACCATTTCCCGCTGCGCTTACCTGCACCGAAACCGAAGGCGCCCTACa                                                                                                               >  1:112613/1‑274 (MQ=255)

                                                                                                                                                               tgCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCCCTACAGccc                                                                                                           >  2:815770/1‑277 (MQ=255)

                                                                                                                                                               tgCCG‑CCGGGCCGGGATAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGc                                                                                                             <  3:733666/275‑1 (MQ=255)

                                                                                                                                                                 ccg‑ccgGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATTAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACCGGTTGCACCCTTTCCCCCTGCGCTTACCTGCCCCGACACCGAAGGCGCTCTACAGcc                                                                                                            >  1:201277/1‑274 (MQ=255)

                                                                                                                                                                 ccg‑ccgGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCCC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCAATTCCCGCTGCGCATACCTGCCCCGAACCCGCAGGCGCCCTACCGccc                                                                                                           >  3:86786/1‑275 (MQ=255)

                                                                                                                                                                      cgggccgggcTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTAAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCa                                                                                                                                                                                                                                                                     <  2:268120/119‑1 (MQ=255)

                                                                                                                                                                             ggcTAACCGTTCAGGTTGGTATCTTGAGAACCGCG‑CGCCAAATATTGGGTCGATCATCCGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATcc                                                                                                <  3:202016/275‑1 (MQ=255)

                                                                                                                                                                             ggcTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGATGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATTCGcc                                                                                             >  2:490552/1‑278 (MQ=255)

                                                                                                                                                                                        cAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATCTCCCGCTGCGCTTACCTGCAACGACACCGAAGGAGCTCTACAGGCCGTGTGAGATCCGCCATCCGGCg                                                                                     >  2:95074/1‑275 (MQ=255)

                                                                                                                                                                                          ggTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGAt                                                                                   <  1:418963/275‑1 (MQ=255)

                                                                                                                                                                                              ggTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGtt                                                                            >  2:6164/1‑278 (MQ=255)

                                                                                                                                                                                              ggTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGtt                                                                            <  2:508314/278‑1 (MQ=255)

                                                                                                                                                                                               gTATATGTATAACCGCG‑AGACAAAGATTGAGTCGATCAGCGGCCATCGGCCGCTGGGATATAAGCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCg                                                                              <  3:201276/275‑1 (MQ=255)

                                                                                                                                                                                                  tctgattaccctgg‑cgcCAAATATGGGATCGGTCAGCAGCACTCAGGCGCGGGAATATAACCGTTACCTGAATAAGCTGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAAcc                                                                        <  4:25245/265‑1 (MQ=255)

                                                                                                                                                                                                     gTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAAcc                                                                        <  3:253215/275‑1 (MQ=255)

                                                                                                                                                                                                     gTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGACGCTGGAATATAACCGTTACCGGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTAGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCCCTACAGCCCGTGTGCGATCCTCGATCTGGCGATTATCGCTCACCggg                                                                     >  2:257407/1‑278 (MQ=255)

                                                                                                                                                                                                         aaCCGCG‑CGGCAAAGATTCAGTCGATAACACGCCATCAGCCGCTGGAATATGACCGGTTCCTGAAAAAACGGGTGGCGTGGGCATGGGTTAACTGCCTGCTG‑ACCTGGCGCAG‑CCGTTTGGATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTTCGCTTACCTCCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAata                                                                 <  4:193796/278‑1 (MQ=255)

                                                                                                                                                                                                         aaCCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCAGCCATCTTGCGATTATCGTTAACCTGGAata                                                                 >  2:794436/1‑278 (MQ=255)

                                                                                                                                                                                                              cg‑cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGcc                                                                                                                                                                         >  4:234975/1‑169 (MQ=255)

                                                                                                                                                                                                              cg‑cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGTATATg                                                               >  1:531620/1‑275 (MQ=255)

                                                                                                                                                                                                              cg‑cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCCCTACAGCCCGTGTGAGATCCGCCCTCTGGCGATTATCGTTAACCTGGAATATg                                                               >  1:300919/1‑275 (MQ=255)

                                                                                                                                                                                                               g‑cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCg                                                                                                                                                                        <  2:234975/169‑1 (MQ=255)

                                                                                                                                                                                                                 cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGAccc                                                           >  2:530996/1‑277 (MQ=255)

                                                                                                                                                                                                                 cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGAc                                                             >  3:282633/1‑275 (MQ=255)

                                                                                                                                                                                                                 cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCCGGCGATTATCGTTAACCTGGAATATGa                                                              >  1:254269/1‑274 (MQ=255)

                                                                                                                                                                                                                 cgcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCCCTCCCCACCCCGTGTGAGATCCGCCATCCGTCGATACGCGTTAACCTGGAATATGTcca                                                           >  4:52867/1‑276 (MQ=255)

                                                                                                                                                                                                                  gcCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGAcc                                                            <  1:620272/275‑1 (MQ=255)

                                                                                                                                                                                                                               ctgagcatggcacatgagccGGTGGAAACAAACCGTTACCCGAAGAAACTGGTGGCGTGGGCATGGTTTACCGGCCTTCTG‑ACCTCGCGGAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGGGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCg                                            <  4:327317/266‑1 (MQ=255)

                                                                                                                                                                                                                                     agcagccatcagccGGTGGAATATAACCGTGACCTGACTAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGGAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCaa                                         <  1:84693/273‑1 (MQ=255)

                                                                                                                                                                                                                                         gccatcagccGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACCCCGAAGGCGCTCTCCAGCCCGTGTGAGATCCGCCCTCTGGCGCTTATCGTTAACCTGGAATATGCCCCGACAGCGGAGTTCCCCACTCAgg                                    >  4:295501/1‑276 (MQ=255)

                                                                                                                                                                                                                                         gccatcagccGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAggtg                                  >  2:327645/1‑278 (MQ=255)

                                                                                                                                                                                                                                             tcagccGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGttt                                                                                                                                                                                                                                >  4:751617/1‑84 (MQ=255)

                                                                                                                                                                                                                                             tcagccGCTGGAATATAACCGTTACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAACATGACCCGACAGCGGCGTTCCGAAATCCggtg                                  >  1:221474/1‑274 (MQ=255)

                                                                                                                                                                                                                                                             aaCCGTTACCGTGATAAACGGGGGGCGGGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATAGTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCg                 <  4:359971/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ttgcgtGAAAAAACGGGGGGCGGGGGCATGTTTTAACGGCCTGCGG‑ACCTCGCGCAC‑CCGCTTGTATATTAAAGGTAACTGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCAGTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTgg         <  4:798722/273‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ttACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTTCAGGAGATGGTCGCCGACGTGTCGCAACATTTCACGCTGCTCTAACCTGCCCCGACACCGGAGGCGCCCTACAGCCCCGGTCGTATCCGCCAACTGGCGATTAATGTTTACCTGGAACAGTACCCGCGAACCGCGTTCTGCAATCCGGTGGTTCATTTCgcattgcgtcca             >  3:23959/1‑263 (MQ=255)

                                                                                                                                                                                                                                                                  ttACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTTCACCGACACCGAAGGCGCCCTACAGCCCGTGTGAGACCCGCCATCTGGCGATTATCGCTAACCTGGAATATGACCCGACAGCGGCGTTCAGCAAGCAGGTGGTGCATTGCGATTTTCGTAAGCTg          >  2:532107/1‑277 (MQ=255)

                                                                                                                                                                                                                                                                   tACCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCt           >  3:806586/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                    aCCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGCAGGCGCTCTACAGCCCGAGTGAGATCCGCCATCTGGCGATTATCGTTAACCCGGAATATGACCCGACAGCGGCGTTCCGCACGCCGGTGGTTCCTTTCGCTTTCCGTAACCTg          >  3:332893/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                    aCCTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATGATCGTCAACCTGGAATATGACCCGACAGCCGCGTTCCGCCATCAGGTGGTTCATTTCGATTTCCGTACGcgg          >  3:878108/1‑273 (MQ=255)

                                                                                                                                                                                                                                                                    aCCTGAATAAACGGGGTGCGTGGGCATGGTTGAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGAt       <  2:343822/278‑1 (MQ=255)

                                                                                                                                                                                                                                                                      cggAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCTCGCAC‑CAGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTgg         <  1:746576/272‑1 (MQ=255)

                                                                                                                                                                                                                                                                      cTGAATAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTgg         <  1:604771/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tGAATAAACGGGTGGCGTTGGCATGGTTTACCGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGAt       <  3:748316/275‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaTAAACGGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTTCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCGGGTGGTGCATTTCGATTTCCGTAAGCTGGATGt     >  1:281442/1‑275 (MQ=255)

CGCGTCGCAATAACCTTAGCGTCTCCGCCAGTCCGCAGGATATCGGCGTGCTGACGCGTAAGCTGTATGCCGCGTTTGAAGCATTACCAGGTAAAGTGACGCTGGTAAACCCGCAGATTTCACCCGATCTCTCGGAACCGAATCTGACCTTTATTTATGTGCCG‑CCGGGCCGGGCTAACCGTTCAGGTTGGTATCTGTATAACCGCG‑CGCCAAATATTGAGTCGATCATCAGCCATCAGCCGCTGGAATATAACCGTTACCTGAATAAACTGGTGGCGTGGGCATGGTTTAACGGCCTGCTG‑ACCTCGCGCAC‑CCGTTTGTATATTAAAGGTAACGGCATTGTCGATTTGCCTAAGTTGCAGGAGATGGTCGCCGACGTGTCGCACCATTTCCCGCTGCGCTTACCTGCACCGACACCGAAGGCGCTCTACAGCCCGTGTGAGATCCGCCATCTGGCGATTATCGTTAACCTGGAATATGACCCGACAGCGGCGTTCCGCAATCAGGTGGTGCATTTCGATTTCCGTAAGCTGGATGTCTT  >  NC_000913/3992354‑3992894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`