| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,888,219 | T→A | 10.1% | intergenic (+27/‑216) | mnmE → / → tnaC | tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,888,219 | 0 | T | A | 10.1% | 285.6 / 19.3 | 89 | intergenic (+27/‑216) | mnmE/tnaC | tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide |
| Reads supporting (aligned to +/- strand): ref base T (31/49); new base A (4/5); total (35/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-02 | |||||||||||
AACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCAGAATAGACAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGAGGATAAGTGCATTATGAATATCTTACATATATGTGTGACCTCAAAATGGTTCAATATTGAC > NC_000913/3887945‑3888482 | aaCAGGGCAATGGCTTGGACACAAACATGGGAGGCGGTTTCAGGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAAATTTGGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACt < 4:478260/275‑1 (MQ=255) ccaagtattGGTCTTTGACCCCAACATGGAAGGCGGCGTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAGCAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGGCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACt < 4:400297/266‑1 (MQ=255) ctttagaacCCACACGGTAGGGGGCTTCCTAGCGGGGCGTGCCGCCCTACAGGGGCTGGAACAGGCAGCGGAACATCNAAAGCGGGGCAAAGCGCAACTTTTGGGAGCCTGGGCAGGTGAACTGCTTGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTCAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgct < 3:176409/268‑1 (MQ=255) cgtgaacacAAACAGGGAGGGGGTCTCCCNAGGGCGTCGTCGAAACCTAGAGGGGNGGGAACAGGCAGCGGAAAATGTACAACAGGGGAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGNTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTGCTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGGGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgct < 3:137580/270‑1 (MQ=255) gACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGc > 2:410128/1‑267 (MQ=255) ccAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgct < 4:410126/267‑1 (MQ=255) cAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGCAGAGTTGCGTCTGGCACAGCCGAACATAAGCGAAATCTCCGGGGAATTTACTTCAGACGAACTGCTGGGGCGGATTTTCTCCTGCTTCTGTATTTGTAGGTAACCGCACTTACGAAGCCGCATTCTGACTGTAAC‑ATACGGCTTCCCTTCAt > 4:226061/1‑275 (MQ=255) ggagcgcgggctacTGGCGCGTCGTCGAAACCGACAGGACACGGAACAGGCTGCGGGACATCTACAACAAGGCAAGGAGCTACTGTGTGAAGCCAGGGTAGGTGAAAGGCTAGCGGAGCAGTTGAGTCTGGAAAAGCAGAACTTAAGCGAAATCACAGGGGTATTGACTGCGGATGACCTGCAGGGGCGGATTTTATCCAGCTTCGGTAGTGGTAAGTAACCGCGCTTGCGAAGCCGTATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATGGTTAc < 4:140655/262‑1 (MQ=255) aGGCGGGTTCCTGGCGGGGCGTGGCCACCTACCGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCAc < 4:187116/275‑1 (MQ=255) aGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCAc < 2:473112/275‑1 (MQ=255) ggcggcTTCCTGGGGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGGAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACt < 2:385466/275‑1 (MQ=255) cTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa > 2:659786/1‑265 (MQ=255) ccccggCGGGTCGTCGCCCCCGCCAGGCGCTTGAACAGGCAGCGGAACATCTACACCAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGGATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa < 4:565978/271‑1 (MQ=255) cTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCAc < 4:659784/265‑1 (MQ=255) tGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTAGCTTCATTTTTACCACTCCTGTTATTc > 1:139084/1‑275 (MQ=255) aCAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTg < 3:106158/203‑1 (MQ=255) aGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGCTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGCGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTACCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGGTTCATTGTTACCACTCCTGTTGTTCCTCAACCtttttt > 2:189314/1‑251 (MQ=255) taaaacaGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTACAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGAGGCTTCGCTTCATTGTTACCAc < 2:244074/213‑1 (MQ=255) ggCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt > 2:87925/1‑250 (MQ=255) aaGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGt < 4:87924/250‑1 (MQ=255) aaCTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCAtt > 1:104185/1‑190 (MQ=255) gTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa < 3:104184/190‑1 (MQ=255) gggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGNGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTTTTATTCCTCANCCCTTTTTTTAAACATTAAAATTTTTTATTAATTTATAATTTTTTNANACAAAATTTAATATTTCTTCCCaaaaaa > 3:82591/1‑272 (MQ=255) gggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTg > 2:515669/1‑176 (MQ=255) ggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATGTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc < 2:539106/179‑1 (MQ=255) aGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc < 4:515667/176‑1 (MQ=255) gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc < 7:787038/149‑1 (MQ=255) gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgct > 4:773549/1‑159 (MQ=255) gAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAg < 7:586213/149‑1 (MQ=255) aCTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgc < 2:773551/159‑1 (MQ=255) tgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAGAATGCGgc < 7:107136/149‑1 (MQ=255) gCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgct > 6:301240/1‑148 (MQ=255) ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGCATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAGAATGCGgctcc < 7:747457/148‑3 (MQ=255) ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcg > 7:446869/1‑149 (MQ=255) ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAGAATGCGgctcc > 8:747457/1‑146 (MQ=255) agagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTa < 1:82592/274‑1 (MQ=255) gagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgcttc < 7:699577/149‑1 (MQ=255) gagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgcttc > 7:403391/1‑149 (MQ=255) gTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTAGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa < 7:289525/149‑1 (MQ=255) cTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc > 5:770807/1‑148 (MQ=255) ggCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑NTGCGGCTTCGCTTCATTGTTACCNCTccn > 4:722972/1‑152 (MQ=255) ggCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt > 4:73813/1‑172 (MQ=255) acaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTc < 6:556704/148‑1 (MQ=255) cagcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa < 2:722974/153‑1 (MQ=255) cagcagAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTa < 2:73814/172‑1 (MQ=255) agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGCCGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa < 8:81264/137‑1 (MQ=255) agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa > 7:81264/1‑137 (MQ=255) aCTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAtt < 5:289474/148‑1 (MQ=255) tAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatg < 6:872652/112‑4 (MQ=255) tAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatg > 5:872652/1‑109 (MQ=255) gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc < 7:428262/149‑1 (MQ=255) cGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc > 5:898433/1‑148 (MQ=255) tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt < 6:770807/148‑1 (MQ=255) tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt < 5:749890/148‑1 (MQ=255) ccGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttttt > 7:700488/1‑149 (MQ=255) gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa < 5:301240/148‑1 (MQ=255) ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa < 6:779999/148‑1 (MQ=255) ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt > 7:245155/1‑149 (MQ=255) ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt > 3:74473/1‑152 (MQ=255) ccAGACGACCTGCTGGGGCGGATTTTCTTCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACCTTAAAATTc < 8:779670/148‑1 (MQ=255) gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGt < 1:74474/152‑1 (MQ=255) tgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat < 8:446869/149‑1 (MQ=255) cGGTTTTTTTCCAGGTTTTGTATTTTGAANTATCCTCATTTACAAAACCGAATTANGACTGTCAG‑ATGCTGCTTCGCTTCATTGTTACCTCTCCTGTTATTTCTCAACCCTTTTTTTATACATTATAAATCTTACTNTATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATGTAAACAATTTCAGAATAGACAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGAGGATAAGTGCAtt < 3:139083/275‑1 (MQ=255) ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCtt < 8:403391/149‑1 (MQ=255) tttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa > 6:117227/1‑148 (MQ=255) tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa < 8:178024/149‑1 (MQ=255) ccAGCTTCTGTAGTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAGTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCAGAATAGACAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGAGGATAAGTGCATTATGAATATc < 2:313123/274‑1 (MQ=255) tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTaa < 6:898433/148‑1 (MQ=255) gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAata < 8:700488/149‑1 (MQ=255) gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAata < 8:847879/149‑1 (MQ=255) ttGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTg < 7:648139/149‑1 (MQ=255) ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGAGTCACATTTAAACAATTTCAGAATAGACAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGCGGCTAAGTGCATTATGAATATCTTACATATAtgtgtg > 1:154770/1‑275 (MQ=255) taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCTTTTTTTTAAACATTaaa < 6:669369/101‑1 (MQ=255) taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCTTTTTTTTAAACATTaaa > 5:669369/1‑101 (MQ=255) agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc > 2:762982/1‑93 (MQ=255) taaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTg > 2:59565/1‑54 (MQ=255) taaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt > 4:371982/1‑70 (MQ=255) aCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa < 4:762980/93‑1 (MQ=255) cgcgCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc < 4:59564/54‑1 (MQ=255) cgcgCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt < 2:371984/70‑1 (MQ=255) gcgcTTACGAAGCCGCATCCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAACATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCAGAATAGATAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGAGGATAAGTGCAtt > 1:11052/1‑240 (MQ=255) aCGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc > 7:879317/1‑140 (MQ=255) aCGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc < 8:879317/140‑1 (MQ=255) gCCGCA‑TCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgat > 6:765557/1‑144 (MQ=255) gCCGCA‑TCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgat < 5:765557/144‑1 (MQ=255) gCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAGACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCAGAATAGACAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGAGGATAAGTGCATTATGAATATCTTACATATATGTTTGACCTCAAAATGGTTCAATATTGac > 2:156635/1‑274 (MQ=255) catCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa > 6:204973/3‑102 (MQ=39) catCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa < 5:204973/100‑1 (MQ=39) aCTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTaaa < 8:245155/149‑1 (MQ=255) aCTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTaaa < 8:373290/149‑1 (MQ=255) aCTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTaaa > 8:133194/1‑149 (MQ=255) | AACAGAGCATGGGCTTTGACACCAACATGGAAGGCGGCTTCCTGGCGCGTCGTCGCCACCTACAGGCGCTGGAACAGGCAGCGGAACATCTACAACAGGGCAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGATTCGATTCACATTTAAACAATTTCAGAATAGACAAAAACTCTGAGTGTAATAATGTAGCCTCGTGTCTTGCGAGGATAAGTGCATTATGAATATCTTACATATATGTGTGACCTCAAAATGGTTCAATATTGAC > NC_000913/3887945‑3888482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |