BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	657,533	A→T	6.9%	intergenic (+32/+22)	cspE → / ← flc	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,533	0	A	T	6.9%	552.2 / 21.7	157	intergenic (+32/+22)	cspE/flc	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand): ref base A (81/65); new base T (6/5); total (87/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.18e-01

Only 100 of 163 total aligned reads displayed.

TATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTC  >  NC_000913/657267‑657793

tATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAACCGTAATCGCTCTGTAAACTACGTCAACAAAAATTAAAAACCCCCATaaacctgtct                                                                                                                                                                                                                                                                >  4:202464/1‑267 (MQ=255)

      tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                                                                                                                                          <  2:70694/275‑1 (MQ=255)

                                      aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt                                                                                                                                                                                                                                                              >  4:155499/1‑239 (MQ=255)

                                          tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                                                                                                                                          <  2:155500/239‑1 (MQ=255)

                                              gTTAATGGGTTGAATGAGTGCGAAGGATTCGTTTGTATGACTCCGGACGACGGCGTCAAAGACGTGTTCGTACACTTCTCGTCACACCAAACTAATGGTTTTAATCTGCGTGCTGAAGGTCAGCGCGTAGAGTTCGAAAGCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGTTACGTCAGCAAGAATTCAAATCCCGCTTAATCAGCTGGTTTTTTCTTGTCTTTAGTGTGCGGTTGAGGACGAAAACATCCAGaa                                                                                                                                                                                                                  <  4:619245/275‑1 (MQ=255)

                                                  aGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCAGAAAACAGCCAGAATGcc                                                                                                                                                                                                              >  2:79209/1‑275 (MQ=255)

                                                                 ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGCCTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGACTGCCA‑GGTAGGTCATTGCa                                                                                                                                                                                              >  2:650120/1‑275 (MQ=255)

                                                                              tttCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTTTGCGGTTGAGGCCGACAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCACCGaa                                                                                                                                                                                 >  4:553470/1‑275 (MQ=255)

                                                                                 cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAACACAGCCAGAATGCAA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGt                                                                                                                                                                              >  2:398425/1‑275 (MQ=255)

                                                                                   ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGATGAAGGTCAGCGCGTAGAGTTCGAACTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTACTCGCTCTGTAAGATACGTCAGCAAGAATTCAACACCCGCTTAATCAGCGGGTTTTTTTTGGTATTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGACA‑GTGCGGTCATGCAAACAGACCCCAGAAGGTTg                                                                                                                                                                            >  1:13926/1‑275 (MQ=255)

                                                                                      cTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                                                                                                                                                                                                    <  2:802800/185‑1 (MQ=255)

                                                                                          ggAAGACGGACGGCAAGACGTTTTCGTAAACTTGTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGTCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGGCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaa                                                                                                                                                                      <  4:254517/274‑1 (MQ=255)

                                                                                                 ggCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCg                                                                                                                                                                                                       >  2:496585/1‑234 (MQ=255)

                                                                                                                     acacTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATTCCA‑GTGCGGTCATGGCAAAAGACCCAAGAAGGTTGCGGAAAAGGGTCAGTAATCCCAAGCCAAAGGGGc                                                                                                                                          >  4:579224/1‑275 (MQ=255)

                                                                                                                       acTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt                                                                                                                                                                                                                                                                       >  7:166554/1‑149 (MQ=255)

                                                                                                                         ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                                                                                                                                                                                                                                                                     >  8:736958/1‑149 (MQ=255)

                                                                                                                            tctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                                                                                                                                                                                                  <  7:447330/149‑1 (MQ=255)

                                                                                                                               gCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                                                                                                                                                                                               >  7:903769/1‑149 (MQ=255)

                                                                                                                                  aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑Gt                                                                                                                                                                                                          <  2:450861/198‑1 (MQ=255)

                                                                                                                                  aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                                                                                                                                                                                             <  5:453461/148‑1 (MQ=255)

                                                                                                                                   tCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            <  2:491070/275‑1 (MQ=255)

                                                                                                                                    ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGt                                                                                                                                                                                                                                                               <  5:754999/144‑1 (MQ=255)

                                                                                                                                    ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGt                                                                                                                                                                                                                                                               >  6:754999/1‑144 (MQ=255)

                                                                                                                                     cAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                                                                                                                                          <  5:217510/148‑1 (MQ=255)

                                                                                                                                     cAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                                                                                                                                                                                         <  7:74342/149‑4 (MQ=255)

                                                                                                                                       gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                                                                                                                                                                                        >  6:223111/1‑148 (MQ=255)

                                                                                                                                        aCTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTgg                                                                                                                                                                                                                                                      >  1:42255/1‑149 (MQ=255)

                                                                                                                                        aCTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                                                                                                                                                                                       >  6:514529/1‑148 (MQ=255)

                                                                                                                                        aCTAATGGCTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTCACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGACTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACCGCCAGAACGCCA‑GTGCGGCCATGGCAAAAGACCCCAGAAGGCTGACGCAAACGTCCAGGAATGCCCAGCCAAAGCGCCCCACTTGTAACAAAAacac                                                                                                                       >  4:99497/1‑275 (MQ=255)

                                                                                                                                        aCTAAGGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac                                                                                                                       <  2:553472/275‑1 (MQ=255)

                                                                                                                                            aTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCtt                                                                                                                                                                                                                                                  <  3:42254/149‑1 (MQ=255)

                                                                                                                                            aTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGt                                                                                                                                                                  <  4:605946/228‑1 (MQ=255)

                                                                                                                                                ttttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                                                                                                                                                                                               >  6:191094/1‑148 (MQ=255)

                                                                                                                                                    aaaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAATCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGCGTGc                                                                                                                                                                                                                                          >  8:564088/1‑149 (MQ=255)

                                                                                                                                                     aaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCg                                                                                                                                                                                                                                         >  7:545418/1‑149 (MQ=255)

                                                                                                                                                      aaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCgg                                                                                                                                                                                                                                        >  7:71901/1‑149 (MQ=255)

                                                                                                                                                        ctctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGt                                                                                                                                                                                                                                       <  5:514529/148‑1 (MQ=255)

                                                                                                                                                             gCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc                                                                                                                                                                                                                                 >  7:33645/1‑149 (MQ=255)

                                                                                                                                                              cTGAAGGTCAGCGCGTAGAGTTCGAACTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGGAAGATACGTCAGCAAGAATTCAAAACCCGCGTAAGCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgag                                                                                                 <  2:99498/275‑1 (MQ=255)

                                                                                                                                                               tGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCATCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg                                                                                                                                                                                                                               <  7:564088/149‑1 (MQ=255)

                                                                                                                                                                gAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTTCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAACACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCGTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGaa                                                                                               <  1:107407/275‑1 (MQ=255)

                                                                                                                                                                gAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc                                                                                                                                                                                                                                                    <  6:165386/127‑1 (MQ=255)

                                                                                                                                                                       aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGCCGAAAACGTTCAGTAATTCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTCCCGAAAATGTTGCAAAGtttgtt                                                                                        >  4:403893/1‑275 (MQ=255)

                                                                                                                                                                        gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                                                                                                                                                                                      >  7:703728/1‑149 (MQ=255)

                                                                                                                                                                        gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                                                                                                                                                                                      <  8:789804/149‑1 (MQ=255)

                                                                                                                                                                         cgcgTAGAGTTCGAAATCATTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑g                                                                                                                                                                                                           >  4:793484/1‑158 (MQ=255)

                                                                                                                                                                         cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                                                                                                                                                                                          <  5:576401/144‑1 (MQ=255)

                                                                                                                                                                         cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                                                                                                                                                                                          >  6:576401/1‑144 (MQ=255)

                                                                                                                                                                                            cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGATTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTGTCTTTAGTGTGCGATTGAGGCCGAAAACAGCCAGAATGCGA‑GTGCGGTCATTGCAAAAGACCCCAGAATGTTGAAGAACACGTTCAGTAATGCCAATCCAAAGCATCTCCCTTTTAACAAAAACACCACTTTTGCGCAACATGTTGAGACGGTTGTTAGACCGCCACACC‑ATCCgg                                                                    >  4:355039/1‑273 (MQ=255)

                                                                                                                                                                                             tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATgg                                                                                                                                                                                                <  8:545418/149‑1 (MQ=255)

                                                                                                                                                                                                  gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCaaa                                                                                                                                                                                            <  6:899968/148‑1 (MQ=255)

                                                                                                                                                                                                   tGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGGCTTTAGGGTTCGGGTGGGGCCGAAAACAGCCAGAATGCCAGGTCGGGTCATGG‑AAAAAACCCCAGAAGGTTGACGGAAAAGGTTAGGAATGCCCAAcc                                                                                                                                                  >  2:181162/1‑189 (MQ=255)

                                                                                                                                                                                                     ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCctc                                                                                                                                      >  4:578212/1‑199 (MQ=255)

                                                                                                                                                                                                        aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                                                                                                                                                                                               <  8:432001/92‑1 (MQ=255)

                                                                                                                                                                                                        aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                                                                                                                                                                                               >  7:432001/1‑92 (MQ=255)

                                                                                                                                                                                                         aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGt                                                                                                                                  <  2:578214/199‑1 (MQ=255)

                                                                                                                                                                                                              cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAg                                                                                                                                                                                <  5:786799/148‑1 (MQ=255)

                                                                                                                                                                                                                tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGtt                                                                                                                                                                             <  8:166554/149‑1 (MQ=255)

                                                                                                                                                                                                                tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGtt                                                                                                                                                                             <  8:33645/149‑1 (MQ=255)

                                                                                                                                                                                                                  tgctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGa                                                                                                                                                                           <  8:301396/149‑1 (MQ=255)

                                                                                                                                                                                                                         aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaaa                                                                                                                                                                     <  5:191094/148‑1 (MQ=255)

                                                                                                                                                                                                                           cGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                   <  6:373812/148‑1 (MQ=255)

                                                                                                                                                                                                                            gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                 <  7:24341/149‑1 (MQ=255)

                                                                                                                                                                                                                            gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGt                                                                                                                                                                  <  5:674593/148‑1 (MQ=255)

                                                                                                                                                                                                                               aTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCa                                                                                                                                                               >  5:879470/1‑148 (MQ=255)

                                                                                                                                                                                                                                 cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaa                                                                                                                                                                      <  5:45919/139‑1 (MQ=255)

                                                                                                                                                                                                                                 cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaa                                                                                                                                                                      >  6:45919/1‑139 (MQ=255)

                                                                                                                                                                                                                                    tctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAAt                                                                                                                                                          >  6:216530/1‑148 (MQ=255)

                                                                                                                                                                                                                                      tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGc                                                                                                                                                        <  6:191521/148‑1 (MQ=255)

                                                                                                                                                                                                                                         aaGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAg                                                                                                                                                    >  8:772631/1‑149 (MQ=255)

                                                                                                                                                                                                                                           gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc                                                                                                                                                   >  6:753363/1‑148 (MQ=255)

                                                                                                                                                                                                                                           gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc                                                                                                                                                   >  5:250990/1‑148 (MQ=255)

                                                                                                                                                                                                                                                gTCAGCAAGAATTCAACACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGACCGAAAACAGCCAGAATGCTA‑GTCCGGCCATTGCAAAAGACCCCAGACGGTTGACAAAAACATTCCTTAAGTCCCAGCCAAACCGGCCCCCTCGGAACAAACACACCAATGCCGCCTAACATGTTGAGAAGGCTGTTA‑ACACGCACAAACATCCGCAGGTGATTAATCATTTCCACACGGGAAACAAGTTCGTCATCCGGCTGatacat               >  4:684368/1‑269 (MQ=255)

                                                                                                                                                                                                                                                gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaa                                                                                                                                                                       >  1:176208/1‑123 (MQ=255)

                                                                                                                                                                                                                                                gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                 >  4:717696/1‑129 (MQ=255)

                                                                                                                                                                                                                                                gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGc                                                                                                                                             >  1:151637/1‑149 (MQ=255)

                                                                                                                                                                                                                                                gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATg              >  2:509489/1‑275 (MQ=255)

                                                                                                                                                                                                                                                 tCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCg                                                                                                                                            <  7:772631/149‑1 (MQ=255)

                                                                                                                                                                                                                                                  cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc                                                                                                                                                                                                                                                    >  2:156423/1‑45 (MQ=255)

                                                                                                                                                                                                                                                  cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTTTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCAGGTGGTGATACATACCTCACACACTGGATCAATGTTCGTCATCCTGCTGAACCGTGCg            >  2:604551/1‑275 (MQ=255)

                                                                                                                                                                                                                                                  cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACtt                                                     >  2:368923/1‑234 (MQ=255)

                                                                                                                                                                                                                                                  cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTc                                 >  2:663199/1‑254 (MQ=255)

                                                                                                                                                                                                                                                  aaGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTGTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCg            <  2:281824/274‑1 (MQ=255)

                                                                                                                                                                                                                                                    gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGcc                                                                                                                                         <  3:151636/149‑1 (MQ=255)

                                                                                                                                                                                                                                                    gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGaa                                                                                               <  2:587823/191‑1 (MQ=255)

                                                                                                                                                                                                                                                      gaGAAATCAAAACCGGCTTAATCAGCGGGGTTTTTTTGTGCTGTAGTGTGAGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAGGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCGGCCGAAAATGTTGCGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCa                             <  4:663197/253‑1 (MQ=255)

                                                                                                                                                                                                                                                       aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct                                                                                                                                       >  5:134019/1‑148 (MQ=255)

                                                                                                                                                                                                                                                         aaTTCAAAACCCGCTTAATCAGCGGGCTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCctct                                                                                                                                     >  5:463456/1‑148 (MQ=255)

                                                                                                                                                                                                                                                           ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtccta     <  2:307421/273‑1 (MQ=255)

                                                                                                                                                                                                                                                             cAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTa                                                                                                                                 <  6:673364/148‑1 (MQ=255)

                                                                                                                                                                                                                                                              aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc                                                                                                                                                                >  6:797186/1‑116 (MQ=255)

                                                                                                                                                                                                                                                                 aCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            >  8:205716/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                 aCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            >  7:226981/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                  cccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            >  6:292540/1‑148 (MQ=255)

                                                                                                                                                                                                                                                                    cGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAac                                                                                                                         >  7:490639/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                     gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATga                                                                                                                                                        >  7:675762/1‑116 (MQ=255)

                                                                                                                                                                                                                                                                     gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATga                                                                                                                                                        <  8:675762/117‑2 (MQ=255)

                                                                                                                                                                                                                                                                     gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGAGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCAATTCTGCCGAAAATGt                                                                                                     >  4:160310/1‑168 (MQ=255)

                                                                                                                                                                                                                                                                      cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATg                                                                                                                                                         <  5:191511/115‑1 (MQ=255)

                                                                                                                                                                                                                                                                        tAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcacc                                                                                                                      >  5:306684/1‑148 (MQ=255)

TATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTC  >  NC_000913/657267‑657793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`