| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,170,282 | C→G | 7.3% | W31C (TGG→TGC) | ldtC ← | L,D‑transpeptidase linking Lpp to murein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,170,282 | 0 | C | G | 7.3% | 270.6 / 11.0 | 84 | W31C (TGG→TGC) | ldtC | L,D‑transpeptidase linking Lpp to murein |
| Reads supporting (aligned to +/- strand): ref base C (36/41); new base G (3/3); total (39/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01 | |||||||||||
GACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTT > NC_000913/1170038‑1170532 | gaCGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCAGGTagc‑gccagc > 2:31291/1‑275 (MQ=255) cAATGTTGATCACAATGCCTTCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTTGCGGCAGCGGCCAGGGGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAA‑GGCAgc > 2:15755/1‑274 (MQ=255) aTCACAATGCCTTCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCTGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACg > 2:481780/1‑274 (MQ=255) ccagtGCCTTCGGGGGGGGCATCGGGAAGTAGGGTTTGCAACGGGAGCGTTAACACGCGGCCCGCGGGCGGTACGTANGGGTCAAAGCCGGGGTTAGCCTGTAACAGAGCGGGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTaaa < 3:163180/271‑1 (MQ=255) tGCCTTCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAT‑GGCAGCGGCGAACGTAAAAAAc > 2:489372/1‑275 (MQ=255) tCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACAACTGCCCGATGGCGGCAGCGGCCAGGGGTTGGCTTTTTCCGG‑AGCAGCCAGCGCCCCGGGCAGC‑GCGAACGAaaaacaaggttg > 4:494020/1‑267 (MQ=255) cgcgcgGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGACCGTAAAAAACGTTAg > 2:376543/1‑274 (MQ=255) gcgcATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTCTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCga > 2:643946/1‑275 (MQ=255) gTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGat > 2:13028/1‑260 (MQ=255) cccGCTGCCCGCGCGCGGTACGTAAGGATCAACGACGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat < 2:494022/273‑1 (MQ=255) cacGCTGCCCGGGCGCGGTAAGTAATGATCAACGCCCGGGTTAGCCTGTTCCAGAGCGAGCAAGCCGCCGTTGTATTTTTTTGCGATGGCTTCCAGAGAACCACCTTCATTTTCCACCGCGTGAAATTTG‑TTTTCGCCAACCAGACGACGGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat < 4:489370/275‑1 (MQ=255) tGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCACCCAGACGACTGCCCGCTGGCGGCCGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCCGCGGAGAACGTAAAAAACGCTAGACAGCGACAAACAGGCGTCTTGAGCTGACCCAACAATCAATaataaaca > 4:83303/1‑273 (MQ=255) gTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGcc > 8:463028/1‑149 (MQ=255) gTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGc < 5:334807/148‑1 (MQ=255) tAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCCGACGACTGCCCGCTGGCGGCAGCGGTCa < 7:112557/149‑1 (MQ=255) aaGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAg > 8:327415/1‑149 (MQ=255) aaGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAg > 7:636782/1‑149 (MQ=255) ggATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAgg > 6:57456/1‑148 (MQ=255) gATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGCACGTCAACAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAg > 2:293907/1‑275 (MQ=255) tgtaatgCCGGGGTTCGCCTTGAATAGGGCGAGGAAGCCGACGTTTTACTTTTTGGCGATGGCTTCCAGAGAACAACCGTCATTTTCCACCACATGAGATTTG‑TTTTCGCCAACCAGACGACCGCCCGCTGGCGGCAGCTGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa < 4:376541/269‑1 (MQ=255) tCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa > 2:189723/1‑243 (MQ=255) tCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAAAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACCGAt > 2:594106/1‑275 (MQ=255) aaCGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTgg > 8:396301/1‑149 (MQ=255) aaCGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTgg > 7:67981/1‑149 (MQ=255) aaCGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTgg < 8:373801/149‑1 (MQ=255) aaCGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa > 2:271849/1‑241 (MQ=255) aCGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTGATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTgg > 6:431226/1‑148 (MQ=255) ccGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat < 4:271848/241‑1 (MQ=255) tAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTa < 2:496025/274‑1 (MQ=255) aGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGt < 5:429320/148‑1 (MQ=255) aGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTa < 7:210166/149‑1 (MQ=255) aGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCAAGAAAAACGCGTTTTGATCATCCCACAAAATcct > 4:170138/1‑228 (MQ=255) tGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACAACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat < 2:170139/229‑1 (MQ=255) caaagggggaAAGCCGATGTTGTATTATTTGGGAAGGGCTACCAGGGAACCACCGTGATTTTCCACCACATGAAATTTG‑TTTGCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAAGTGTTGGCTTTTGACGGTAGC‑GCCAGCGCAAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGa < 4:594104/266‑1 (MQ=255) aagagCGATAAATCCGACGTTGTATTGTCTGGAGATAGCTGCCAGAGAACAACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGa < 2:83304/273‑1 (MQ=255) gagCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTagc‑gccagcgcc > 7:885336/1‑149 (MQ=255) agCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa > 2:814373/1‑217 (MQ=255) gagaAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTGATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑g < 5:431226/148‑1 (MQ=255) gagaAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑gg < 7:396301/149‑1 (MQ=255) ngaAANCCNACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCNCCCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat < 4:814371/216‑1 (MQ=255) agaAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGc < 4:767985/111‑1 (MQ=255) agaAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGaa > 4:425093/1‑183 (MQ=255) agaAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa < 2:546248/213‑1 (MQ=255) aaggccgcagtgttATTTTTTTGCGTTTGGTTCCAGAGACCGACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGa < 4:481778/262‑1 (MQ=255) aaaaCCTACGTTGTATTTTTTGGCGATGGCTGCCAGAGAGCCACCGTCATTTTCCACCACATGAAATTTG‑TTTACGCCGACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGa < 4:293906/275‑1 (MQ=255) aGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAAc < 2:425095/183‑1 (MQ=255) gATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGc < 5:518308/148‑1 (MQ=255) aTGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGcc < 6:63669/148‑1 (MQ=255) ggCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGc > 7:661845/1‑149 (MQ=255) gCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTGTTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAg > 6:7076/1‑148 (MQ=255) ttCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCgag > 7:639548/1‑149 (MQ=255) agaACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGaaaaa < 5:57456/148‑1 (MQ=255) accGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGttt < 8:254755/149‑1 (MQ=255) gTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGa < 8:639548/149‑1 (MQ=255) tCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGat < 8:636782/149‑1 (MQ=255) ttttCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatca > 5:149248/1‑148 (MQ=255) ttCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca < 8:885336/149‑1 (MQ=255) ccacATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg > 4:457449/1‑168 (MQ=255) aTGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAAt < 5:912588/148‑1 (MQ=255) aTGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTa < 2:457451/168‑1 (MQ=255) tGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATc > 6:755055/1‑148 (MQ=255) gAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCacaacccc < 6:149248/148‑5 (MQ=255) gAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATcc < 5:755055/148‑1 (MQ=255) gAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATcc < 5:650502/148‑1 (MQ=255) ttG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat > 6:365889/1‑148 (MQ=255) ttG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat > 5:780976/1‑148 (MQ=255) cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATaa < 7:463028/149‑1 (MQ=255) gCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg < 8:67981/149‑1 (MQ=255) caacaccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg > 8:826173/7‑149 (MQ=255) cgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAACGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGtt < 1:133454/274‑1 (MQ=255) acTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaat > 5:263399/1‑148 (MQ=255) tggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaataa > 5:100954/4‑148 (MQ=255) tGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGTAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATcc > 1:49577/1‑121 (MQ=255) tGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaataa < 5:365889/148‑1 (MQ=255) ggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTg > 6:841049/3‑103 (MQ=255) ggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTg < 5:841049/101‑1 (MQ=255) cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa < 7:94713/149‑1 (MQ=255) cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATNGTAATAAACAGATAAGCCTGAAt > 3:99398/1‑162 (MQ=255) cGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataa < 3:49576/121‑1 (MQ=255) gCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGAt < 7:327415/149‑1 (MQ=255) ctgccgccAGCGGGCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATa < 7:826173/142‑1 (MQ=255) cTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTatg < 1:99399/162‑1 (MQ=255) tgccgccAGCGGGCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATa < 6:100954/142‑1 (MQ=255) tGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc > 4:808389/1‑143 (MQ=255) gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAg < 6:630346/148‑1 (MQ=255) ggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGAt < 2:808391/143‑1 (MQ=255) gCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTa > 6:163394/1‑148 (MQ=255) ccAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa > 3:64515/1‑103 (MQ=255) ccAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTat > 6:433547/1‑148 (MQ=255) | GACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGCGCATCTGGAAGTAGGGTTTGCAACGGGATCGTTAACACGCTGCCCGCGCGCGGTACGTAAGGATCAACGCCGGGGTTAGCCTGTAACAGAGCGAGAAAGCCGACGTTGTATTTTTTGGCGATGGCTTCCAGAGAACCACCGTCATTTTCCACCACATGAAATTTG‑TTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGC‑GCCAGCGCCAC‑GGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTATGGATGGTGACAGTGTCGGATAGTGCAGGGAAGTGCAAAGAATTTGTAAATGTTGCAGATGGGGGCGCAGAAACGCCCCCGATTTACCATTAAGCGATCGCGTT > NC_000913/1170038‑1170532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |