| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 83,590 | G→T | 9.4% | intergenic (‑61/+32) | leuA ← / ← leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 83,590 | 0 | G | T | 9.4% | 281.4 / 17.0 | 92 | intergenic (‑61/+32) | leuA/leuL | 2‑isopropylmalate synthase/leu operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (40/43); new base T (5/4); total (45/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-03 | |||||||||||
TACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGAT > NC_000913/83320‑83857 | tACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACATCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTGTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑GATCGAAAATAATGACTTGCTGGCCCATCGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCCTTTCGGGCATAAACAAACCACCGCAATGgcgc > 4:219902/1‑275 (MQ=255) gcgggcgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACCCAAGCTTGCCTGTAACGCNTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGTTCTGTGGCTCTTTTAAAGGGCCNCGCTTCGGGCCTAAAAAAACCCCCCNACTTGGCCGGGGTTtttttttggtgtgtg > 3:135357/1‑268 (MQ=255) cgATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGCTTTTTGTTTGACTGCGTGCTg > 2:482904/1‑274 (MQ=255) gTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCggg > 2:263161/1‑247 (MQ=255) aaaTCGCCCGGGGAAGAGACGGGGAAACCGACTTCCAGACGGTAAACACCCCAACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTGTCCTTCACACTCGAGCTTGCCTGTACCGCCTGTTTACCGTCGCGCAAGTTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCt < 4:333041/274‑1 (MQ=255) cccGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGt > 2:11454/1‑275 (MQ=255) gCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTAc > 4:653973/1‑275 (MQ=255) tctcaaacACGTCAACACCCATACGCTCAAGTGCCAGCGCAATTTTCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGAAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGttt < 4:663896/267‑1 (MQ=255) tgcataacgaagacacGCGTACGCTGAAGGGCCAGGGCAATTTTCAG‑TTTTACTTTCGCACCCAAGGTTGCCGTTAAGGACGGTTCACTGTCGGGGAATGGGG‑GAACGAAAATAGTGGCTTGCTGGCTCATGGTTTGTGTCCTTGACTCTTTTAGGGGGCCGGGGTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTGACTCGTCTACCGCGCAAAGAAGATGCGTTtagt < 4:72046/263‑1 (MQ=255) tCCATCCCGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag < 2:613206/274‑1 (MQ=255) gccagaccggcAACACCCATGCGGCTAAGGGCCAGAGGAATTTGCAG‑TTTTTCTTTCCCGCGCAAGCATGCCTGGAACGCCTGTTCACCGTGGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTGAGAGCGCCTCGTTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtag < 4:54357/264‑1 (MQ=255) tACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGcgcg > 4:87146/1‑178 (MQ=255) cTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtt < 2:87147/178‑1 (MQ=255) cTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGGAGACCGATAAAGCGAAc > 2:527834/1‑275 (MQ=255) aaGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCCGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCACAGAAGATGCGTTTAGTAGTAGTAGACCGATAAACCGAACGa > 2:131460/1‑274 (MQ=255) gggCCAGCGCCATCTGCAG‑TTTTTCTTTCACCCACAAGCTTGCCTGTCTCGCCTGTTCTCCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCTCCTCGCTTCGTGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGAATGCCGCTCACTCGTCTACcgcg < 1:83791/228‑1 (MQ=255) gccagggagaTTTGCGG‑TTTTTCGGCCAGACTCAAGTCGGCCGTGAAAGGCGGTTTACCGTCGCGGAAGTTGG‑TATGGAAAATAATGGCTTGCTGGCTCATGGTTTGGGTCCTTGGCTCTTTTAGAGCGTGTGGATTCGGGGATAAAAAAACCCGCGCAATGGGACGGGTTTTTTGTTTGACTGCGTGCTGGCTTAAGGCTGGAGGCCGCTCACTCGTCTACCGCGCAAAGAAGGTGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa < 4:482902/266‑1 (MQ=255) gCCAGCGTAATTCGAAG‑TTTTTCTTTCACCCTCAAGATTGCCTGTAAGGCCTGTTCACCGTCGAGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTGTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa < 4:339112/275‑1 (MQ=255) gccaaggcaTTTTCAGTTTTTTCTTTCACACTCAAGCTGGCCTGTACCGCCTTTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCGCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGTGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGa < 4:527832/267‑1 (MQ=255) aGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGc > 4:346822/1‑182 (MQ=255) aGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGAATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATCCGTTTCGTAGTAGTAGACCGATAAAGCGAACGATTTGAGTc > 1:183998/1‑275 (MQ=255) gcgcAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCCTGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCTTGCTGGCTTAATGGTGGATGCCGCTCACTCTTCTCCCGCGCAAAGCAGATGCGTTTAGTAGTAGTCGACCGATAAGGCGAACGATTGGAGtac > 4:393820/1‑273 (MQ=255) cAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGc < 2:346824/182‑1 (MQ=255) aTTTGCTT‑TTTTTCTGTCACATGCACGCGTTCGATTAACGCTGTTTCCCCGTCGGGGAA‑GTGGTTATCGTAATTAATNTGTTGCTTGCTGATGGTTGGGCGCCTTCTCTCTTTTAGAGCGCCTCCCTTCGGGCATAAAAATACCCGCGAGATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCTAACGATGTGAGTCATTaaa < 3:183997/275‑1 (MQ=255) ttttCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTgcgc > 6:425912/1‑148 (MQ=255) tctttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCggg < 5:261845/148‑1 (MQ=255) ctttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCCATTGCGCGGGt < 5:425912/148‑1 (MQ=255) tttcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttt > 8:149884/1‑149 (MQ=255) tcACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGCCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt < 8:21583/149‑1 (MQ=255) cTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt > 6:336289/1‑148 (MQ=255) tCAATCTTGCCTGTAAAGCCTTTTCACCGGCGCGCAAGGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCGTTTAGGGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata < 4:11454/275‑1 (MQ=255) tCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata < 2:653975/275‑1 (MQ=255) ttGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGACGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCttt < 1:135358/275‑1 (MQ=255) tGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCg > 6:302816/1‑148 (MQ=255) gCCTGTAATGCCTGTTCATCGTCGCGCAATGTGG‑CACCGAAAATAATGACTTGCTGGCTCACGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCCTGTTTCGGGCATAAAAAAACCCGCGCAATGgcgtg > 4:589852/1‑126 (MQ=255) gCCTGTAACGCCTGTTCACCGTCGCGCGATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGATCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGATTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAACGAAGATGCGTTTAGTAGTAGTAGAGCGATAAAGCGACCGATGTGCGTCATTAAATCAGCTCCAGATGACTGCGATATGCTTTTa > 2:141549/1‑275 (MQ=255) cTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGc > 4:361758/1‑261 (MQ=255) gTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtt < 2:589854/128‑1 (MQ=255) aaactcTGTTTACTGGCGCGCAATGTGA‑GATCGAAAATAATGCTGTTCTGGTACATGGTTTGGGTCCTTGTCTCTTTTAGAGGGCCTCGCTTCGGGAATAAAAAAACCCGCGGAAGGGCGCGGGTTTTTTGTTTGACTGCGTTCTGGCTTGATGCTGGATGCCGCTCACTCGGCTACCGCGCAGAGAAGATGCGTTAAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTa < 4:131459/270‑1 (MQ=255) aaCGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCg > 2:358748/1‑164 (MQ=255) aaCGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata < 2:361760/261‑1 (MQ=255) ccTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCa < 4:358746/164‑1 (MQ=255) cTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa < 7:125097/147‑1 (MQ=255) cTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa < 7:434351/147‑1 (MQ=255) cTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa > 8:434351/1‑147 (MQ=255) cTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTaa > 8:125097/1‑147 (MQ=255) ttCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGAt < 2:449089/183‑1 (MQ=255) cACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTgg < 5:336289/148‑1 (MQ=255) gcgcAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgcg < 4:280110/103‑1 (MQ=255) aaaaTAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGACGCTCACTCGTCTACCGCGCAAGGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCTAGATGAATGCGAAATGCTTTTAGAGTTACTGGATACCAAACCGGATGACAACCCTTAccc > 4:383585/1‑272 (MQ=255) aatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTa > 1:116880/1‑199 (MQ=255) aatGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATaaa > 2:563215/1‑275 (MQ=255) gACTTGCTGTCTCATGGTTTGGGTCCTTGGCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATaaaaa < 2:219903/274‑1 (MQ=255) gACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAaagaag < 5:302816/148‑1 (MQ=255) aCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATc < 3:116879/199‑1 (MQ=255) tGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGc > 6:573672/1‑148 (MQ=255) cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGcgcg > 2:673866/1‑75 (MQ=255) cTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCg > 1:118103/1‑125 (MQ=255) cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGtt < 4:673864/75‑1 (MQ=255) cTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTa < 3:118102/125‑1 (MQ=255) tCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt < 5:573672/148‑1 (MQ=255) aTGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt < 6:186454/75‑1 (MQ=255) aTGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGttttt > 5:186454/1‑75 (MQ=255) tGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGc > 1:165648/1‑197 (MQ=255) tCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGata < 3:165647/197‑1 (MQ=255) ccTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGa > 6:416197/1‑148 (MQ=255) cTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCAGCCAGCGGGGGTTCTGccac > 2:365054/1‑272 (MQ=255) tGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTTCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCg > 1:160698/1‑275 (MQ=255) agCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGTTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTTGTAGTAGACCGATAAAGCTAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTATTGGAAAAAAAAACGGATGTCAAACCCTACGCAATAAAAACGTTCCGCCAAGTGGAGTTCTGTCACCGTAAACTTTGCTa > 4:180477/1‑275 (MQ=255) gcgctccgattCGGGCATAAAAAAACCCGCGCAATGGGGCGGGTTTTTTGTTTGCCTGCGGGCTGGCTTAAGGCTGGAGTCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTaa < 4:563213/266‑1 (MQ=255) cgcCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCt > 2:711385/1‑74 (MQ=255) ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCAtt > 4:801065/1‑152 (MQ=255) ccTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATtg > 2:409779/1‑275 (MQ=255) ttgCTTCGTGCAGACAAAAACCCTGGCCATGTGACGTGTTTTTTGTTGGATTGCGTGCTTGCTTAATGCTGGATGCCNCTACCGCGTCTACAGCGCAAAGAAGATGCGTTTAGTATTAGTAGACCGATACAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATtgtg < 3:160697/273‑1 (MQ=255) tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGAt < 4:711383/74‑1 (MQ=255) tCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCAt < 7:149884/149‑1 (MQ=255) gCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAAt < 2:801067/152‑1 (MQ=255) ttCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTa < 6:219610/147‑1 (MQ=255) ttCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTa > 5:219610/1‑147 (MQ=255) tCGGGCATAAAACAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCg < 2:180478/275‑1 (MQ=255) cGGGGATAAAAAAACCCGGTGCAGGGGGCGGGTTTTTTGTTTGACTGAGTGCTGGCTTAAGGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCg < 4:409777/274‑1 (MQ=255) aCCCGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGa > 6:431069/1‑148 (MQ=255) cccACGCAATGGGGCGGGTTTTTCGTGTGAATGCGTACTGGTTTAAGGCTGGATGCCGCCCACTCGTCGAACGGGGTAACAAAATTCGGTAAGTAGAGGTGGACGGATAAAGCAAACGGGGTGAGTCTTTAAAGCAACGCCAGATGAAGGCGATATGCTTTTAGGGTGACTAGATACAAAAACGGATGGCGACCCTGATGCAATAAAAACGTCCGGGCGGCGTGAGTTCTGCATCCGTAAAATTAGCTATTTGTGCTGCGGTGGTTAGAGTAAg < 4:410125/274‑1 (MQ=255) ccGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCa > 6:361969/1‑127 (MQ=255) ccGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCa < 5:361969/127‑1 (MQ=255) ccGCGCCATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATg > 7:648349/1‑149 (MQ=255) ccGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCg < 2:393822/275‑1 (MQ=255) gcgcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGtt > 2:788804/1‑87 (MQ=255) gcgcAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTANATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCNATAAAAACGTCCCGCCAGCGTGAGTTTTTCATCCGGAAAATTAGCNANATTTGCTTCGGGGGTTAAAGTAAGcata > 3:68657/1‑272 (MQ=255) aaTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTtagt < 4:788802/87‑1 (MQ=255) aaTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGatat > 8:505404/1‑149 (MQ=255) aTGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGGTGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGatat > 5:152844/1‑148 (MQ=255) | TACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAG‑TTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGG‑TATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGATGCCGCTCACTCGTCTACCGCGCAAAGAAGATGCGTTTAGTAGTAGTAGACCGATAAAGCGAACGATGTGAGTCATTAAATCAGCTCCAGATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGAT > NC_000913/83320‑83857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |