| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,706,121:1 | +T | 10.5% | coding (1004/1800 nt) | lepA ← | back‑translocating elongation factor EF4, GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,706,121 | 1 | . | T | 10.5% | 387.6 / 48.2 | 105 | coding (1004/1800 nt) | lepA | back‑translocating elongation factor EF4, GTPase |
| Reads supporting (aligned to +/- strand): ref base . (45/49); new base T (4/7); total (49/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
ACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAG > NC_000913/2705974‑2706268 | aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑c > 4:887893/1‑149 (MQ=255)aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc > 1:252945/1‑148 (MQ=255) cGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑c > 2:108408/1‑148 (MQ=255) gATAACTTCTCTTGACGTGGTTTCAACTTCATACACTCCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcg > 3:305781/1‑149 (MQ=255) gATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgc > 1:147090/1‑148 (MQ=255) aTAACTTCTCTTGACGTGGTTTCAACTTCATAGACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAGAGC‑CCAgcgc > 4:774167/1‑149 (MQ=255) aaCTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcgcg < 2:252945/148‑1 (MQ=255) aaCTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcgcg > 2:135560/1‑148 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcgc > 4:801989/1‑144 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcgc < 3:801989/144‑1 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTgg > 3:841709/1‑149 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTg < 1:135560/148‑1 (MQ=255) tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTgg > 1:51213/1‑148 (MQ=255) tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGa < 3:90767/149‑1 (MQ=255) ttGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCtt > 4:299921/1‑149 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt < 2:402398/148‑5 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt < 2:280112/148‑5 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCttt < 4:306783/149‑1 (MQ=255) gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCttt < 2:136997/148‑1 (MQ=255) gTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCgg > 4:269979/1‑149 (MQ=255) gTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCg < 1:610/148‑1 (MQ=255) tGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCgg < 2:813616/148‑1 (MQ=255) gTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTc > 4:546370/1‑149 (MQ=255) gTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTc < 4:762382/149‑1 (MQ=255) ttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCttt < 3:844329/140‑1 (MQ=255) ttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCttt > 4:844329/1‑140 (MQ=255) ttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcgc < 4:186611/129‑1 (MQ=255) ttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAgcgc > 3:186611/1‑129 (MQ=255) tCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt > 2:621872/1‑134 (MQ=255) tCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt < 1:621872/138‑5 (MQ=255) tCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATa > 4:731923/1‑149 (MQ=255) cAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGa < 3:902653/133‑1 (MQ=255) aCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGa > 2:281940/1‑148 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt < 1:733045/133‑5 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt > 2:733045/1‑129 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAAc > 1:750900/1‑148 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 3:174333/1‑149 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 4:312625/1‑149 (MQ=255) tCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 2:254945/1‑148 (MQ=255) cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt < 3:335761/149‑1 (MQ=255) cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt < 4:417392/149‑1 (MQ=255) cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt < 3:259509/149‑1 (MQ=255) aNACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg > 3:148849/1‑149 (MQ=255) tACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTg < 2:750900/148‑1 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAg > 1:568208/1‑145 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAg < 2:568208/145‑1 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa < 1:281940/148‑1 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg < 3:887893/149‑1 (MQ=255) aCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑cc > 2:82966/1‑108 (MQ=255) aCGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑cc < 1:82966/108‑1 (MQ=255) tCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGtt < 1:254945/148‑1 (MQ=255) cGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa < 4:841709/149‑1 (MQ=255) gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTAGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAgg < 4:604672/149‑1 (MQ=255) cgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAgg > 2:578441/1‑148 (MQ=255) gcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCAt > 1:329726/1‑124 (MQ=255) gcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCAt < 2:329726/124‑1 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa < 4:305781/149‑1 (MQ=255) gtggtgATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa < 2:147090/148‑1 (MQ=255) gATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTAc < 4:149169/149‑1 (MQ=255) aTCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt > 1:510675/1‑101 (MQ=255) aTCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggctt < 2:510675/105‑5 (MQ=255) aTCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTgggctt < 2:203448/104‑5 (MQ=255) aTCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTgggctt > 1:203448/1‑100 (MQ=255) tCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTAccc > 3:354018/1‑149 (MQ=255) agatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCa < 1:203774/148‑1 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCa > 3:444074/1‑110 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCa < 4:444074/110‑1 (MQ=255) atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCTATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcg > 3:805032/1‑149 (MQ=255) tccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcgc < 3:269979/149‑1 (MQ=255) tccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcgc < 4:191212/149‑1 (MQ=255) cagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGt < 4:320813/149‑1 (MQ=255) atcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCAc < 3:312625/149‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCa < 1:286068/148‑1 (MQ=255) tCCAGACGTTCCTGGATGATCTGCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGATGGCTTCAt < 1:783480/148‑1 (MQ=255) tCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCAt > 2:615816/1‑148 (MQ=255) cAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAgt > 3:503070/1‑149 (MQ=255) cGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAgtcgt < 2:646151/148‑1 (MQ=255) cGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACC‑AAGC‑TCAGCGCGCTGCAGCTTTCCGGCTCATCGAGCAGTGAGGCATCGTTCATGCTGAGTTTACCCAGCGGGTCACGGAAGGCCTCACAgacgtc < 2:869133/148‑1 (MQ=255) tGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACt > 3:716588/1‑149 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACtt > 2:424844/1‑148 (MQ=255) atCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCgg > 3:314850/1‑149 (MQ=255) ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAAGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCggg > 2:397060/1‑148 (MQ=255) gCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGtat < 1:108408/148‑1 (MQ=255) aGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCgg < 2:629080/139‑1 (MQ=255) aGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCgg > 1:629080/1‑139 (MQ=255) gAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGCCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCg < 3:774167/149‑1 (MQ=255) gCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGt < 1:450805/148‑1 (MQ=255) gCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGt < 1:578441/148‑1 (MQ=255) ccGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGttt < 4:815440/149‑1 (MQ=255) ccGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGtt < 2:51213/148‑1 (MQ=255) cAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGa > 2:906736/1‑148 (MQ=255) gAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCt > 4:27051/1‑149 (MQ=255) ccAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaa < 1:906736/148‑1 (MQ=255) ccAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaa > 2:611235/1‑148 (MQ=255) cAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAg > 3:395716/1‑149 (MQ=255) cAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAg > 4:154461/1‑149 (MQ=255) aaGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGcc < 3:627072/149‑1 (MQ=255) aaGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGcc < 4:805032/149‑1 (MQ=255) aaGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGcc > 3:894258/1‑149 (MQ=255) c‑ccAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAg < 1:615816/148‑1 (MQ=255) | ACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAG > NC_000913/2705974‑2706268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |