BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,092,774:1	+A	12.1%	coding (897/951 nt)	gshB →	glutathione synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,092,774	1	.	A	12.1%	357.7 / 52.8	99	coding (897/951 nt)	gshB	glutathione synthetase
Reads supporting (aligned to +/- strand): ref base . (44/43); new base A (8/4); total (52/47)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

Only 100 of 104 total aligned reads displayed.

GACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAA  >  NC_000913/3092628‑3092921

gACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑Tc                                                                                                                                                   >  4:680747/1‑149 (MQ=255)

  cTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGa                                                                                                                                                 <  4:439909/149‑1 (MQ=255)

   tGGAAAATCGTCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGa                                                                                                                                                 <  1:252180/148‑1 (MQ=255)

   gggAAAACCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGAt                                                                                                                                                <  4:484504/148‑1 (MQ=255)

            gCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCCCCAGCCCAAACTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGATCGA‑CACCGGaa                                                                                                                                        <  1:204952/148‑1 (MQ=255)

                 tCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTa                                                                                                                                   >  1:763388/1‑148 (MQ=255)

                  cAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t                                                                                                                                                    <  2:800018/130‑1 (MQ=255)

                  cAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAAt                                                                                                                                 <  3:633078/149‑1 (MQ=255)

                    gATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATgg                                                                                                                               >  3:281053/1‑149 (MQ=255)

                     aTCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATgg                                                                                                                               >  2:299782/1‑148 (MQ=255)

                      tCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGaa                                                                                                                                        >  4:480263/1‑138 (MQ=255)

                      tCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGaa                                                                                                                                        <  3:480263/138‑1 (MQ=255)

                         ggCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGc                                                                                                                           <  1:113990/148‑1 (MQ=255)

                           ccGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCa                                                                                                                         <  1:433456/148‑1 (MQ=255)

                            cGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCAt                                                                                                                        >  2:384406/1‑148 (MQ=255)

                             gACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t                                                                                                                                                    <  1:429824/119‑1 (MQ=255)

                             gACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t                                                                                                                                                    >  2:429824/1‑119 (MQ=255)

                                gCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGaa                                                                                                                    <  1:738516/148‑1 (MQ=255)

                                      agaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGACGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGt                                                                                                              <  2:384255/148‑1 (MQ=255)

                                      agaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGtt                                                                                                             <  4:297657/149‑1 (MQ=255)

                                       gaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGtt                                                                                                             >  1:48951/1‑148 (MQ=255)

                                           aaGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcag                                                                                                        >  4:530032/1‑149 (MQ=255)

                                              ggCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcag                                                                                                     >  4:581623/1‑149 (MQ=255)

                                                cTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                                   <  3:449906/149‑1 (MQ=255)

                                                cTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagc                                                                                                    <  1:753683/148‑1 (MQ=255)

                                                    tttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTaa                                                                                               >  3:455450/1‑149 (MQ=255)

                                                     ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagca                                                                                                      <  3:151178/141‑1 (MQ=255)

                                                     ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagca                                                                                                      >  4:151178/1‑141 (MQ=255)

                                                     ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTaa                                                                                               >  1:718432/1‑148 (MQ=255)

                                                     ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTaa                                                                                               >  2:750175/1‑148 (MQ=255)

                                                              tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg                                                                                     >  3:784137/1‑149 (MQ=255)

                                                                tGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCg                                                                                   >  4:257623/1‑149 (MQ=255)

                                                                  gATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCgag                                                                                 >  4:882249/1‑149 (MQ=255)

                                                                    tatCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCgaga                                                                                <  1:293676/148‑1 (MQ=255)

                                                                       catcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                            >  3:35378/1‑149 (MQ=255)

                                                                        atcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                            <  1:384406/148‑1 (MQ=255)

                                                                          cGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtc                                                                          >  1:819075/1‑148 (MQ=255)

                                                                           ggCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTTGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtctc                                                                        <  3:779464/149‑1 (MQ=255)

                                                                            gCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCg                                                                       >  3:516648/1‑149 (MQ=255)

                                                                             cGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCg                                                                       <  1:249661/148‑1 (MQ=255)

                                                                              gACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGtt                                                                     <  3:882249/149‑1 (MQ=255)

                                                                                  gTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTgaga                                                                 <  4:155256/149‑1 (MQ=255)

                                                                                        ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAg                                                                              <  2:783574/130‑1 (MQ=255)

                                                                                        ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAg                                                                              >  1:783574/1‑130 (MQ=255)

                                                                                        ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTg                                                            <  1:750175/148‑1 (MQ=255)

                                                                                        ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                           >  3:249547/1‑149 (MQ=255)

                                                                                          gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGActc                                                              >  1:420898/1‑144 (MQ=255)

                                                                                          gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGActc                                                              <  2:420898/144‑1 (MQ=255)

                                                                                          gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAg                                                          >  1:175550/1‑148 (MQ=255)

                                                                                             aTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGa                                                                                                                                         >  1:23846/1‑66 (MQ=255)

                                                                                             aTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGa                                                                                                                                         <  2:23846/66‑1 (MQ=255)

                                                                                             aTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                      >  4:222908/1‑149 (MQ=255)

                                                                                              ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACa                                                     >  4:801000/1‑149 (MQ=255)

                                                                                              ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACa                                                     <  3:801000/149‑1 (MQ=255)

                                                                                                  cGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAgcgc                                                 <  4:335968/149‑1 (MQ=255)

                                                                                                     cACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGccc                                               <  2:763388/148‑1 (MQ=255)

                                                                                                       ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                            >  4:700050/1‑117 (MQ=255)

                                                                                                       ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                            <  3:700050/117‑1 (MQ=255)

                                                                                                       ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCcttc                                            >  3:125754/1‑149 (MQ=255)

                                                                                                         aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                            <  1:686376/115‑1 (MQ=255)

                                                                                                         aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa                                                                            >  2:686376/1‑115 (MQ=255)

                                                                                                           cccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                         <  2:824614/148‑1 (MQ=255)

                                                                                                              aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                                      >  2:536469/1‑148 (MQ=255)

                                                                                                              aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCAc                                     <  3:680747/149‑1 (MQ=255)

                                                                                                                ccTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGtt                                                                     <  3:690031/115‑1 (MQ=255)

                                                                                                                ccTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGtt                                                                     >  4:690031/1‑115 (MQ=255)

                                                                                                                   gTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCAt                                 >  1:709934/1‑148 (MQ=255)

                                                                                                                       tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                            <  3:388103/149‑1 (MQ=255)

                                                                                                                       tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                            <  3:257623/149‑1 (MQ=255)

                                                                                                                       tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                            <  4:281053/149‑1 (MQ=255)

                                                                                                                       tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTg                             <  1:299782/148‑1 (MQ=255)

                                                                                                                          tGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGGTCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgc                          >  2:666479/1‑148 (MQ=255)

                                                                                                                          tGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgcg                         >  3:98833/1‑149 (MQ=255)

                                                                                                                               ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGtt                    >  4:356177/1‑149 (MQ=255)

                                                                                                                               ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt                     >  2:92107/1‑148 (MQ=255)

                                                                                                                               ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt                     >  1:340317/1‑148 (MQ=255)

                                                                                                                               ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGt                     >  1:388192/1‑148 (MQ=255)

                                                                                                                                  aaGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCt                 >  3:132279/1‑149 (MQ=255)

                                                                                                                                   aGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                           >  4:843195/1‑106 (MQ=255)

                                                                                                                                   aGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa                                                           <  3:843195/106‑1 (MQ=255)

                                                                                                                                    gCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt               >  3:815177/1‑149 (MQ=255)

                                                                                                                                    gCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt               >  4:582331/1‑149 (MQ=255)

                                                                                                                                     ttaacattCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGtt                    >  4:787823/7‑143 (MQ=255)

                                                                                                                                     ttaacattCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGtt                    <  3:787823/137‑1 (MQ=255)

                                                                                                                                     cAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCttt               >  2:392856/1‑148 (MQ=255)

                                                                                                                                       gagTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtttttt            <  3:414766/149‑1 (MQ=255)

                                                                                                                                       gagTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCAGTTGCtttttt            <  3:530032/149‑1 (MQ=255)

                                                                                                                                          tttCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGa          <  2:388192/148‑1 (MQ=255)

                                                                                                                                          attCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga                                                                                  >  3:800570/2‑76 (MQ=38)

                                                                                                                                          attCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga                                                                                  <  4:800570/75‑1 (MQ=38)

                                                                                                                                          attCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa         >  3:475451/2‑149 (MQ=255)

                                                                                                                                           ttCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCGGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa         >  2:877925/1‑148 (MQ=255)

                                                                                                                                           ttCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCGGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa         >  2:228291/1‑148 (MQ=255)

                                                                                                                                           ttCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa         >  1:304947/1‑148 (MQ=255)

                                                                                                                                           ttCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa         >  1:178791/1‑148 (MQ=255)

                                                                                                                                            tCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                                   >  4:286940/1‑57 (MQ=37)

                                                                                                                                            tCCGGTGATCGA‑CACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAcagcagca                                                                                                   <  3:286940/57‑1 (MQ=37)

                                                                                                                                             ccGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAAcc       <  2:819075/148‑1 (MQ=255)

                                                                                                                                              cGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCa      <  2:196116/148‑1 (MQ=255)

                                                                                                                                                 tg‑tCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGTATACTGGGCGCTGTTGCTTTTTTGAACCAGGaa  >  4:596929/1‑149 (MQ=255)

GACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAA  >  NC_000913/3092628‑3092921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`