Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,691,169	A→T	5.3%	noncoding (172/184 nt)	glmY ←	sRNA activator of glmS mRNA, glmZ processing antagonist

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,691,169	0	A	T	5.3%	320.7 / 5.2	91	noncoding (172/184 nt)	glmY	sRNA activator of glmS mRNA, glmZ processing antagonist
Reads supporting (aligned to +/- strand):  ref base A (42/44);  new base T (3/2);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGG  >  NC_000913/2691029‑2691313                                                                                                                                              |                                                                                                                                                 gcagAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTAccc                                                                                                                                           <  1:319025/148‑1 (MQ=255)   agAATCAGCAAAAATGGCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCgg                                                                                                                                         <  2:303630/148‑1 (MQ=255)      aTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCttt                                                                                                                                     <  3:357338/149‑1 (MQ=255)      aTCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCtt                                                                                                                                      <  1:683850/148‑1 (MQ=255)        cAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGGTAATT‑CCCGGCTTTg                                                                                                                                    <  2:91893/148‑1 (MQ=255)         aGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGt                                                                                                                                   <  1:495224/148‑1 (MQ=255)          gCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTa                                                                                                                                 >  4:887885/1‑149 (MQ=255)          gCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTa                                                                                                                                 >  3:737362/1‑149 (MQ=255)           cAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                                <  4:737362/149‑1 (MQ=255)            aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATg                                                                                                                               <  4:871634/149‑1 (MQ=255)            aaaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATg                                                                                                                               >  3:538984/1‑149 (MQ=255)             aaaaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATg                                                                                                                               >  1:914036/1‑148 (MQ=255)               aaTGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGa                                                                                                                             <  1:152925/148‑1 (MQ=255)                     aGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                                <  2:97749/139‑1 (MQ=255)                     aGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTAt                                                                                                                                >  1:97749/1‑139 (MQ=255)                     aGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGc                                                                                                                      >  4:511082/1‑149 (MQ=255)                      gCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCg                                                                                                                     >  4:467506/1‑149 (MQ=255)                        aTTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGt                                                                                                                   <  3:323014/149‑1 (MQ=255)                         ttACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTg                                                                                                                  <  3:343837/149‑1 (MQ=255)                             cAGATGTCGTACTGAGCGGGGAAAAACGGGCCAGCGTGTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCt                                                                                                               >  2:699850/1‑148 (MQ=255)                               gTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAc                                                                                                            >  3:650207/1‑149 (MQ=255)                                ttGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAAc                                                                                                            >  2:1860/1‑148 (MQ=255)                                      tAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACg                                                                                                      <  1:177339/148‑1 (MQ=255)                                            gCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGcc                                                                                               <  4:650207/149‑1 (MQ=255)                                                 gAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATg                                                                                          >  3:638926/1‑149 (MQ=255)                                                   aaaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATgg                                                                                         <  1:204837/148‑1 (MQ=255)                                                   aaaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGt                                                                                        >  4:787696/1‑149 (MQ=255)                                                    aaaCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGtt                                                                                       <  3:22110/149‑1 (MQ=255)                                                      aCGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTg                                                                                      >  2:814705/1‑148 (MQ=255)                                                       cGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGat                                                                                    >  3:340859/1‑149 (MQ=255)                                                            cAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAg                                                                                >  2:476615/1‑148 (MQ=255)                                                            cAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAg                                                                                <  2:255492/148‑1 (MQ=255)                                                              gCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                             >  4:226187/1‑149 (MQ=255)                                                               cGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                             >  1:19975/1‑148 (MQ=255)                                                               cGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTa                                                                             <  1:699850/148‑1 (MQ=255)                                                                 tttCAAGGCGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGc                                                                          >  4:77531/1‑149 (MQ=255)                                                                    cAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTg                                                                        >  2:20792/1‑148 (MQ=255)                                                                        gtgtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATc                                                                   <  4:340859/149‑1 (MQ=255)                                                                         tgtTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATc                                                                   >  1:154748/1‑148 (MQ=255)                                                                               tctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            >  3:902191/1‑149 (MQ=255)                                                                                ctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            >  2:583873/1‑148 (MQ=255)                                                                                ctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            >  2:551570/1‑148 (MQ=255)                                                                                ctcGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGtt                                                            >  2:346409/1‑148 (MQ=255)                                                                                  cGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTgg                                                          <  2:685789/148‑1 (MQ=255)                                                                                   gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGaa                                                               >  4:209158/1‑142 (MQ=255)                                                                                   gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGaa                                                               <  3:209158/142‑1 (MQ=255)                                                                                   gTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGAc                                                        >  4:635611/1‑149 (MQ=255)                                                                                      aGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACgg                                                      >  2:907142/1‑148 (MQ=255)                                                                                      aGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCCACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggc                                                     <  3:77531/149‑1 (MQ=255)                                                                                       gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGc                                                                          >  2:626241/1‑127 (MQ=255)                                                                                       gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGc                                                                          <  1:626241/127‑1 (MQ=255)                                                                                       gACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggca                                                    <  3:226187/149‑1 (MQ=255)                                                                                          gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCTTTGttgta                                                                                                                               <  4:459967/71‑4 (MQ=21)                                                                                          gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑TAATTCCCGGCTTTGttgta                                                                                                                               >  3:459967/1‑68 (MQ=21)                                                                                          gcgAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcaggc                                                 >  3:542501/1‑149 (MQ=255)                                                                                              aTAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCAcc                                              <  1:677751/148‑1 (MQ=255)                                                                                               tAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCt                                             <  1:342597/148‑1 (MQ=255)                                                                                                   cTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgttg                                        <  3:887885/149‑1 (MQ=255)                                                                                                    tGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgttg                                        <  2:914036/148‑1 (MQ=255)                                                                                                      aTGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTtgtg                                      <  1:907142/148‑1 (MQ=255)                                                                                                      aTGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTtgtg                                      <  1:189165/148‑1 (MQ=255)                                                                                                          tAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAgg                                                                                                                       <  4:97378/63‑1 (MQ=255)                                                                                                          tAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAgg                                                                                                                       >  3:97378/1‑63 (MQ=255)                                                                                                           aaCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                         >  4:470803/1‑140 (MQ=255)                                                                                                           aaCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCttgtt                                         <  3:470803/140‑1 (MQ=255)                                                                                                           aaCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCa                                 >  1:230051/1‑148 (MQ=255)                                                                                                            aCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcag                                                   >  3:642032/1‑129 (MQ=255)                                                                                                            aCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACggcag                                                   <  4:642032/129‑1 (MQ=255)                                                                                                            aCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCAtt                               <  3:221204/149‑1 (MQ=255)                                                                                                            aCCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCAt                                <  1:220751/148‑1 (MQ=255)                                                                                                             ccGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTc                              <  3:900317/149‑1 (MQ=255)                                                                                                              cGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTc                              >  1:582952/1‑148 (MQ=255)                                                                                                                     aaGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTa                      <  4:538984/149‑1 (MQ=255)                                                                                                                      aGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTa                      <  2:19975/148‑1 (MQ=255)                                                                                                                      aGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTAt                     <  4:902191/149‑1 (MQ=255)                                                                                                                         tCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGt                   >  1:432780/1‑148 (MQ=255)                                                                                                                             ccataacaaAGCCGGGTAATT‑CCCGGCTTTGTTGTGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCAc              >  3:180565/5‑149 (MQ=39)                                                                                                                             aTACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCa               <  1:346409/148‑1 (MQ=255)                                                                                                                                caacaaAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGt            <  1:583873/148‑1 (MQ=255)                                                                                                                                  acaaAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTcc          >  1:891264/1‑148 (MQ=255)                                                                                                                                     aaGCCGGGTAATT‑CCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa      >  4:657310/1‑149 (MQ=255)                                                                                                                                      aGCCGGGTAATT‑CCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa      >  1:818250/1‑148 (MQ=255)                                                                                                                                      aGCCGGG‑TAATTCCCGGCTTTGTTGTGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa      >  1:353234/1‑148 (MQ=255)                                                                                                                                      aGCCGGG‑TAATTCCCGGCTTTGTTGTGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGaa      >  1:866255/1‑148 (MQ=255)                                                                                                                                      aGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAg     >  4:422751/1‑149 (MQ=255)                                                                                                                                       gCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCTCGTCCCGAAg     <  1:476615/148‑1 (MQ=255)                                                                                                                                       gCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAg     <  1:814705/148‑1 (MQ=255)                                                                                                                                        ccGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAggg   <  3:635611/149‑1 (MQ=255)                                                                                                                                         cGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAgggg  >  3:883211/1‑149 (MQ=255)                                                                                                                                            g‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGat                                                                      >  2:764233/1‑78 (MQ=255)                                                                                                                                            g‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGat                                                                      <  1:764233/78‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                 GCAGAATCAGCAAAAATGCCAGCATTACCAGTTGTCGTAATGAGCGGGGAAAAACGGGCCAGCGTTTCAAGGTGTTACTCTCGTCAGACGCGAATAGCCTGATGCTAACCGAGGGGAAGTTCAGATACAACAAAGCCGGG‑AATTACCCGGCTTTGTTATGGAATAAGGCGGTGCCTAACTCGACGTTTCGCCCGATGGTTGATATAGCTACGCTGATATCAGAAGTTGGACGGCAGGCACCTTGTTGTGCGTCATTCGTATTTTATGTAGCACGTCCCGAAGGGG  >  NC_000913/2691029‑2691313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑