Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,268	0	T	C	24.5%	93.7 / 15.1	50	intergenic (+474/+168)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (19/18);  new base C (10/2);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 8.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATA  >  NC_000913/4296004‑4296538                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                   tgctttGGCGTGATTTTTGAGGGCGGTGTCGGCGTTTATGCCGCAGCCGACACCAACGCCTGATGCGACGCTTGCCGCGTCTTACCAGGCCAAGGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                                                                                                                                                          <  3:273539/272‑1 (MQ=11) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATTCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTCATTTAGCGTGATTTTGTAGG‑TCGGAAAAGGCGTGTAAGCAGCATCCg                                                                                                                                                                                                                                                                          >  1:801435/1‑275 (MQ=255) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCATCGTGATTTTGTAGG‑TCGTGTAAGGCGTTTACGCCGCATCCg                                                                                                                                                                                                                                                                          >  1:161951/1‑275 (MQ=255)             aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATCTACCGTTATTTTGTAGG‑TCGGATAAGCCGCTTACGCCGCCTCCCACATCAATGcg                                                                                                                                                                                                                                                               >  3:590396/1‑273 (MQ=11)             aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACACAGCAAGAGCCTGATTGAGCGTGATTCTGTATG‑TCGGATAAGGAGTTTACGCCGCAGCGGACCTCAAACCCTGa                                                                                                                                                                                                                                                            >  4:627995/1‑277 (MQ=11)                   tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAAACACCGCAATAGCTTGATTTAGCTTGATTTTGTAGG‑TCGGATAGGGCGTTTACGCCGCATCAGACAACACTGCCTgttcgga                                                                                                                                                                                                                                                       >  4:536601/1‑270 (MQ=11)                          gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCCTGATTTTGTAGT‑TCGGCTCAGGCGTTTACGCCGCACCCGCCATCAATGCCAGagcctgcgcttggc                                                                                                                                                                                                                                               >  4:634735/1‑264 (MQ=11)                           aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATTAGGCGTTTACGCCGCATCCGACCTCAAGTCCTGATGCGACCCttg                                                                                                                                                                                                                                                 >  3:649070/1‑273 (MQ=255)                                     tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGG‑TCGGATCAGGCGTTTACGCCGCATCCGACATCAATGACTGATGCGCCGCTTTCCGcttcttc                                                                                                                                                                                                                                       >  3:541587/1‑273 (MQ=11)                                     tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATCAGGCGTTTACGCCCCATCCGACATCAATTCCTGATGCCACCCtgtccgcttcttca                                                                                                                                                                                                                                      >  3:31548/1‑262 (MQ=11)                                           gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGA‑CTTGTGGGTTCGGACAAGGCCTTTACGCCGGATCCGACATCAACGCCCGCTGCCGCGCTTGCCGCGTTCTTTCAGGcc                                                                                                                                                                                                                                >  3:408578/1‑275 (MQ=11)                                             aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCCTTTACGCCGCATCCTACATCAATGCCTGATGCGACGCTTGCCGCGTCCTTTCACGCCtcttc                                                                                                                                                                                                                           >  4:180859/1‑274 (MQ=11)                                                                          gcggcgTCTTAGCAGGCCGACGCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGTTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGAAGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                                                                                                                                                                  <  1:237688/271‑1 (MQ=9)                                                                                                                                                                                aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                                                          >  1:455113/1‑274 (MQ=9)                                                                                                                                                                                          tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTCGCCGG‑TCGGACAAGGCGCTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATAAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGAGCGATTTTGTCGGTCGGATACGGCGTTTAAGC‑‑TGCATCCGACATAAATCCCTGATCAGACGCTTGCCGCGTTTTATCAGTCCTATCTTAACCGTTGGTCAATTTTTCAAAc                                                                                >  3:145215/4‑274 (MQ=11)                                                                                                                                                                                                 tCTTATAAGGCTTCGGCCAGACGGGGCAATAGCCTGATTTAGGGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                     <  2:326861/278‑1 (MQ=14)                                                                                                                                                                                                   ttATCAGGCATACGCCAGACAGCGCAATAGCCTGATTTAGCTTTATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                      <  1:596646/275‑1 (MQ=255)                                                                                                                                                                                                     ttCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                                                     <  1:150619/273‑1 (MQ=255)                                                                                                                                                                                                     aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTTCTCACACCCgtt                                                                  >  2:264919/1‑275 (MQ=255)                                                                                                                                                                                                          gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  2:624426/278‑1 (MQ=255)                                                                                                                                                                                                           ccTACGCCAGATAGGGTAATAGCCTGATTTAGCTGGATTGTGGAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACAGCAACGCCTGATGCGACGCTTAACGCGTCTTATAAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  2:704510/277‑1 (MQ=14)                                                                                                                                                                                                           ccTACGCCAAAAAGCGCAATAGCCTGATTTGGCTTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAGTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAATTCTTCACACCCGGTATCaaa                                                            <  2:648703/277‑1 (MQ=14)                                                                                                                                                                                                             tccGCCAGAAAGCGCAATAGCCGGTTTTAGCGTGATTTTGTAGG‑GAGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  1:900208/273‑1 (MQ=255)                                                                                                                                                                                                              aCGCCAGACAACACAATAGCATGATTTAGCGTTATTTTGTAGG‑TCGGATAAGGCTTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                                                            <  1:289058/274‑1 (MQ=14)                                                                                                                                                                                                               cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCTACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTCTGTAGGTCGGATAAGGCGTTTA‑‑CGACGCATCCGACATCAATGACTGATGAGACCTTTGCCGCGTCTTATCAGGCCTAATTTATCCGTTGGTTAATTTTTCACACTCCTCACCACCGGTATCAAccc                                                          >  3:341502/1‑275 (MQ=11)                                                                                                                                                                                                               cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                                                          >  3:179715/1‑275 (MQ=255)                                                                                                                                                                                                               cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                           >  1:51564/1‑274 (MQ=255)                                                                                                                                                                                                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGTGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAACCCCTTCCTTACTGCt                                             >  2:278861/1‑278 (MQ=255)                                                                                                                                                                                                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTACCAAACCCTTCCTTACCGc                                              >  4:673311/1‑277 (MQ=255)                                                                                                                                                                                                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa                                                >  3:434044/1‑275 (MQ=255)                                                                                                                                                                                                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                           >  1:681037/1‑264 (MQ=255)                                                                                                                                                                                                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                             <  2:627995/278‑1 (MQ=255)                                                                                                                                                                                                                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTCTGTAGGTCGGATAAGGAGTTTATGC‑‑CGAATCCGACATCAATGCCTGTTGCGACGCTTGCCGCGTCTTATCTGGCTTATTTTAACCGTTGGTTAATTTTTCAAACTCTTCACTTCCGGTTTCAAACCCTTCCAttac                                                >  3:312277/1‑272 (MQ=14)                                                                                                                                                                                                                         cgaaaTAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                 <  1:723012/271‑1 (MQ=255)                                                                                                                                                                                                                            aaTAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                             <  1:179715/275‑1 (MQ=255)                                                                                                                                                                                                                            aaTAGCCTGATTTAGCGTGATTTTGTAGG‑GCGCGTAAGACTTTTATGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCTTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                             <  1:722869/275‑1 (MQ=17)                                                                                                                                                                                                                             aTAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                                                       <  3:681037/264‑1 (MQ=255)                                                                                                                                                                                                                                 ccTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                        <  2:536601/275‑1 (MQ=255)                                                                                                                                                                                                                                 ccTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGATAAGGCGTTTAAGC‑‑CGAATCCGACATCAACGCATGATGCGACGCTTGCCGCGTCATCTCAGGCCTATCTTAACAGCTTGTTAATTTTTCAAACACATCACAACCGGTATTATATCCATCCACACATCTCCGGCTCa                                     >  2:356444/1‑278 (MQ=11)                                                                                                                                                                                                                                     aTTTCGCGTGATTTTGTAGG‑TCGGATAAGGCGTGTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAcc                                  <  2:505337/277‑1 (MQ=255)                                                                                                                                                                                                                                      tttAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                   >  3:533589/1‑275 (MQ=255)                                                                                                                                                                                                                                               aTTTTGTTGG‑GCGAATAAGGCGTTTATGCCGCTTCCGACATCAACGCCGTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACAAGTGCAg                           <  3:890380/274‑1 (MQ=18)                                                                                                                                                                                                                                                          tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGTTATCAAACCCTTCCATACCGCTCAGGTTCAACCAGTGCATCTCCTTCTGCt                >  1:62702/1‑275 (MQ=255)                                                                                                                                                                                                                                                            ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTg              >  3:251875/1‑275 (MQ=255)                                                                                                                                                                                                                                                             gATAAGGCGTTTATGCCGACTCCGATATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt                                             <  1:477272/243‑1 (MQ=255)                                                                                                                                                                                                                                                                aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCCATGCACCGCCTTTTCCTTGgtct          >  3:843502/1‑275 (MQ=255)                                                                                                                                                                                                                                                                 aGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttctt         <  1:230106/275‑1 (MQ=255)                                                                                                                                                                                                                                                                       ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCCCCTTTTGCTTGTTCTTCTCGa    >  1:346263/1‑274 (MQ=255)                                                                                                                                                                                                                                                                        tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGAt   >  3:253707/1‑274 (MQ=255)                                                                                                                                                                                                                                                                        tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATa  >  1:664995/1‑275 (MQ=255)                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                   TGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGG‑TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATA  >  NC_000913/4296004‑4296538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑