BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,679,434:1	+C	5.1%	intergenic (‑67/+30)	yphF ← / ← yphG	putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,434	1	.	C	5.1%	695.7 / 32.4	158	intergenic (‑67/+30)	yphF/yphG	putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand): ref base . (77/73); new base C (2/6); total (79/79)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-01

Only 100 of 162 total aligned reads displayed.

AACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAG  >  NC_000913/2679177‑2679683

aaCTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTa                                                                                                                                                                                                                                           <  2:110432/274‑1 (MQ=255)

 aCTGAACTGTTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTAGCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTAc                                                                                                                                                                                                                                          <  2:22193/274‑1 (MQ=255)

                               cATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGc                                                                                                                                                                                                                                                     >  2:272754/1‑233 (MQ=255)

                                            cTGACGCAACCCAGCGTAGTATCCCTGGGTTTCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCa                                                                                                                                                                                              <  4:298925/275‑1 (MQ=255)

                                                 gCACTCCAGGTGAGTACACCTGAGTATCAAGGGTTATGTAGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGTTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATTAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                                                                                                                         <  4:327213/275‑1 (MQ=255)

                                                                                   tatTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTaa                                                                                                                                                       >  2:194847/1‑275 (MQ=255)

                                                                                          ccAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCCCGGTGGTGAATAATGCCGCTTTCGGCAAAGCCGGATTAATCGcct                                                                                                                                                 >  4:149516/1‑273 (MQ=255)

                                                                                          ccAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGt                                                                                                                                                <  4:194847/275‑1 (MQ=255)

                                                                                                              cGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑t                                                                                                                                                                                                                                                          <  7:678829/149‑1 (MQ=255)

                                                                                                               gCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑Tg                                                                                                                                                                                                                                                         >  7:203782/1‑149 (MQ=255)

                                                                                                                   cccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGc                                                                                                                                                                                                                                                     <  6:1085973/149‑1 (MQ=255)

                                                                                                                    ccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCt                                                                                                                                                                                                                                                    >  5:985750/1‑149 (MQ=255)

                                                                                                                    ccTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCt                                                                                                                                                                                                                                                    <  5:1392429/149‑1 (MQ=255)

                                                                                                                     cTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTc                                                                                                                                                                                                                                                   <  6:395804/149‑1 (MQ=255)

                                                                                                                      tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCa                                                                                                                                                                                                                                                  >  7:366663/1‑149 (MQ=255)

                                                                                                                      tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCa                                                                                                                                                                                                                                                  <  7:660166/149‑1 (MQ=255)

                                                                                                                         cAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCatca                                                                                                                                                                                                                                               <  8:734310/149‑4 (MQ=255)

                                                                                                                           aaTAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAatat                                                                                                                                                                                                                                             <  6:583410/149‑1 (MQ=255)

                                                                                                                             tAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTa                                                                                                                                                                                                                                           <  5:388865/149‑1 (MQ=255)

                                                                                                                               gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  7:500775/149‑1 (MQ=255)

                                                                                                                               gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  6:384553/149‑1 (MQ=255)

                                                                                                                               gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  5:1221160/149‑1 (MQ=255)

                                                                                                                                 gcgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGt                                                                                                                                                                                                                                       <  7:1048749/149‑1 (MQ=255)

                                                                                                                                  cgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                                                                                                                                                                                >  4:301205/1‑187 (MQ=255)

                                                                                                                                     tACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAAt                                                                                                                                                                                                                                   >  6:1391528/1‑149 (MQ=255)

                                                                                                                                      aCCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACg                                                                                                                                                                                            <  2:301205/187‑1 (MQ=255)

                                                                                                                                       ccTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCTTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAg                                                                                                                                                                                                                                 <  5:259157/149‑1 (MQ=255)

                                                                                                                                       ccTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGACGCTCAAATATTAGAGTTAATAg                                                                                                                                                                                                                                 >  7:1230517/1‑149 (MQ=255)

                                                                                                                                        cTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAgg                                                                                                                                                                                                                                >  7:611980/1‑149 (MQ=255)

                                                                                                                                        cTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAgg                                                                                                                                                                                                                                >  5:951049/1‑149 (MQ=255)

                                                                                                                                         tAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTa                                                                                                                                                                                                                                           <  7:1398653/137‑1 (MQ=255)

                                                                                                                                          aaaaGCGTAGCCATTAATAATAGATTACGGGTTGATCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTACTAGAGt                                                                                                                                                                                                                              >  7:971409/4‑149 (MQ=255)

                                                                                                                                                gTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTg                                                                                                                                                                                                                        >  8:565659/1‑149 (MQ=255)

                                                                                                                                                 tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGa                                                                                                                                                                                                                       >  7:1337096/1‑149 (MQ=255)

                                                                                                                                                 tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCGCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATAACGGTGGTGAATAATGCGGCTTTCGGCCAAGCCGGATTAATCGACGTCAATTCAGCGCGAGCAGACTAATATTGCTCAACCGCCCCGAGTCCCGCGGACGc                                                                                          >  1:770/1‑274 (MQ=255)

                                                                                                                                                     cATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgc                                                                                                                                                                                                                   <  5:1349103/149‑1 (MQ=255)

                                                                                                                                                      aTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgcg                                                                                                                                                                                                                  >  7:253850/1‑149 (MQ=255)

                                                                                                                                                      aTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGAGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgcg                                                                                                                                                                                                                  >  5:1127215/1‑149 (MQ=255)

                                                                                                                                                       ttAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCgg                                                                                                                                                                                                                 >  8:477354/1‑149 (MQ=255)

                                                                                                                                                       ttAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCgg                                                                                                                                                                                                                 >  5:679063/1‑149 (MQ=255)

                                                                                                                                                       ttAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAACCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCgg                                                                                                                                                                                                                 <  6:656653/149‑1 (MQ=255)

                                                                                                                                                         aataatAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTGATAGGGTTTAGTGAACGCGGtt                                                                                                                                                                                                               <  6:1127215/149‑1 (MQ=255)

                                                                                                                                                            aataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaa                                                                                                                                                                                                            >  7:1212651/1‑149 (MQ=255)

                                                                                                                                                             ataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                                                                                                                                                                           >  8:1115028/1‑149 (MQ=255)

                                                                                                                                                              taGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaaa                                                                                                                                                                                                          <  6:1008557/149‑1 (MQ=255)

                                                                                                                                                                gATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAGATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATa                                                                                                                                                                                                        >  8:874642/1‑149 (MQ=255)

                                                                                                                                                                 aTTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCttt                                                                                                                                                                        >  1:35417/1‑180 (MQ=255)

                                                                                                                                                                  ttACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATaaa                                                                                                                                                                                                      <  7:789090/149‑1 (MQ=255)

                                                                                                                                                                     cGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCAGCTTTCGGACAAGCCGGATGAATCGCCGTCAATTCAGCCCGAGCAGACTCATATTGCGCAACCACCCCGGGGCCCGCGGACGCCAGCATCGcacacaccagcac                                                                     >  4:204454/1‑263 (MQ=255)

                                                                                                                                                                       ggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCa                                                                                                                                                                                                 <  7:434371/149‑1 (MQ=255)

                                                                                                                                                                        gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTaa                                                                                                                                                                                                                                    >  8:720246/1‑113 (MQ=255)

                                                                                                                                                                        gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTaa                                                                                                                                                                                                                                    <  7:720246/113‑1 (MQ=255)

                                                                                                                                                                        gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                                                                                                                                                                                <  6:259928/149‑1 (MQ=255)

                                                                                                                                                                        gttgttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                                                                                                                                                                                >  6:1151248/1‑149 (MQ=255)

                                                                                                                                                                           gttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAgcg                                                                                                                                      >  2:246123/1‑204 (MQ=255)

                                                                                                                                                                            ttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGt                                                                                                                                                                                                                                       >  4:86850/1‑106 (MQ=255)

                                                                                                                                                                              ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                                                                                                                          >  6:864619/1‑149 (MQ=255)

                                                                                                                                                                              ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                                                                                                                          >  8:1412932/1‑149 (MQ=255)

                                                                                                                                                                               tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAAt                                                                                                                                                                                                         >  8:121677/1‑133 (MQ=255)

                                                                                                                                                                               tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACgtgg                                                                                                                                                                                         >  8:1244868/1‑146 (MQ=255)

                                                                                                                                                                                cATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAAt                                                                                                                                                                                                                                   <  2:86850/106‑1 (MQ=255)

                                                                                                                                                                                  tttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                                                                                                                                      <  6:1259298/149‑1 (MQ=255)

                                                                                                                                                                                     ttttAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgctg                                                      <  4:235093/271‑1 (MQ=255)

                                                                                                                                                                                          ggCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc                                                                                                                                                                              <  6:762742/149‑1 (MQ=255)

                                                                                                                                                                                             aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAAt                                                                                                                                                                                                                                   <  6:1253800/93‑1 (MQ=255)

                                                                                                                                                                                             aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                                                                                                                                                                           >  7:1299016/1‑149 (MQ=255)

                                                                                                                                                                                             aTAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGc                                                                                                                                                                           >  8:639194/1‑149 (MQ=255)

                                                                                                                                                                                                   cctcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCga                                                                                                                                                                     >  5:1040615/1‑148 (MQ=255)

                                                                                                                                                                                                         gTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGATTTAGGCCAAGCCGGACTAATCGCCGTCCATTCAGCGCGAGCAGACTCATATTTTGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCCCCTCCATCAGGCTTTTTTCTTTATGCTGCTGTTGTAAATACCCATaaaa                                 >  4:270549/1‑272 (MQ=255)

                                                                                                                                                                                                          tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAACACGGTGGTGAATAATGCCGCTTTCGGCCAAGcc                                                                                                                                                              >  5:431929/1‑149 (MQ=255)

                                                                                                                                                                                                           aGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCg                                                                                                                                                             <  5:1303024/149‑1 (MQ=255)

                                                                                                                                                                                                            gggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGTAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCgg                                                                                                                                                            >  7:1040095/1‑149 (MQ=255)

                                                                                                                                                                                                              gTTTTTATTAACAACGGCTGATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                                                                                                                          >  7:1151021/1‑149 (MQ=255)

                                                                                                                                                                                                                ttttATTAACAACGGCTTATTCTAATTATTGTGTCAGGAGCGGCAGAGCG‑TGCCGCTCAAATAGTACAGGTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGTCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGaca                            <  2:270549/273‑1 (MQ=255)

                                                                                                                                                                                                                  ttattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAAt                                                                                                                                                      <  5:158155/149‑1 (MQ=255)

                                                                                                                                                                                                                   tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATc                                                                                                                                                     <  6:845721/149‑1 (MQ=255)

                                                                                                                                                                                                                     ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc                                                                                                                                                                              <  6:1328733/122‑1 (MQ=255)

                                                                                                                                                                                                                     ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGc                                                                                                                                                                              >  5:1015929/1‑122 (MQ=255)

                                                                                                                                                                                                                          aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCa                                                                                                                                              >  8:44983/1‑149 (MQ=255)

                                                                                                                                                                                                                           acGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCaa                                                                                                                                             <  8:1044906/149‑1 (MQ=255)

                                                                                                                                                                                                                            cGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAt                                                                                                                                            <  8:366663/149‑1 (MQ=255)

                                                                                                                                                                                                                                  ttttttttttttttGTTATGGAGGGCAGNGNT‑TTCCCATAAAAAATTAAAGTTAAAAGGTGTTATTAAACTCGGTANAAAATAAAAGGCATCCCGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCANCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACg        <  3:32173/265‑1 (MQ=255)

                                                                                                                                                                                                                                    tCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGCCGCCCGCATCGCCACCACCCGGCCTTTTTCTTCACGCTGCTGTTGTCAATCCCCATACAGCCCCAACCAGCCAGGTTGCGCGCCgg       >  4:232689/1‑274 (MQ=255)

                                                                                                                                                                                                                                     cTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                                                                                                                                   <  8:564955/149‑1 (MQ=255)

                                                                                                                                                                                                                                     cTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                                                                                                                                   <  7:477354/149‑1 (MQ=255)

                                                                                                                                                                                                                                     cTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa                                                                                                                                   <  5:1378711/149‑1 (MQ=255)

                                                                                                                                                                                                                                        attattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCa                                                                                                                                >  5:636153/1‑149 (MQ=255)

                                                                                                                                                                                                                                        attattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAg  >  1:32173/1‑275 (MQ=255)

                                                                                                                                                                                                                                         ttattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                                                                                                                          <  8:1284580/122‑1 (MQ=255)

                                                                                                                                                                                                                                         ttattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                                                                                                                          >  7:1284580/1‑122 (MQ=255)

                                                                                                                                                                                                                                            ttttGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACt                                                                                                                            >  6:1471657/1‑149 (MQ=255)

                                                                                                                                                                                                                                                gtgATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata                                                                                                                        >  5:1281274/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    tgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc                                                                                                                    <  7:114057/149‑1 (MQ=255)

                                                                                                                                                                                                                                                     gaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGa                                                                                                                                                           >  8:1043164/1‑109 (MQ=255)

                                                                                                                                                                                                                                                     gaGCGGCA‑CGCGCTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcg                                                                                                                   >  8:1198116/1‑149 (MQ=255)

                                                                                                                                                                                                                                                       gCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa                                                                                                                 <  6:956572/149‑1 (MQ=255)

                                                                                                                                                                                                                                                          gCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAcc                                                                                                              <  5:1440854/149‑1 (MQ=255)

                                                                                                                                                                                                                                                             gcgcG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcc                                                                                                           >  8:231670/1‑149 (MQ=255)

                                                                                                                                                                                                                                                              cgcg‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTccc                                                                                                          <  7:44983/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                 g‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGa                                                                                                                                                           <  8:1345917/97‑1 (MQ=255)

AACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAG  >  NC_000913/2679177‑2679683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`