BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,520,504:1	+T	6.8%	intergenic (‑180/+167)	yjhU ← / ← yjhF	putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,520,504	1	.	T	6.8%	897.6 / 63.0	220	intergenic (‑180/+167)	yjhU/yjhF	putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand): ref base . (114/91); new base T (7/8); total (121/99)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 225 total aligned reads displayed.

GCTGCTGTTTGTTTGATCTCTATCCACTTTTGTATACCTGTATTAAACCGGGGAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAA  >  NC_000913/4520298‑4520778

gctgctGTTTGTTTGATCTCTATCCACTTTTGTATACCTGTATTAAACCGGGGAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAACGACGGAGACAT‑G‑ATCCGGCTTTACAGTATTACTGAGAATACCACCATCAACAATAATACAAGGTTCAAAACCCCCCCttt                                                                                                                                                                                                                >  4:106706/1‑275 (MQ=255)

                                                  gggAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATGCGTCACCTTGCAGACGGTTGCCGTCCCTCACTaa                                                                                                                                                              >  1:33240/1‑275 (MQ=255)

                                                                  aaCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCATCTTCCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGA‑ACATGG‑CTCCGGCt                                                                                                                                                                                                                                                                            <  6:374644/149‑1 (MQ=255)

                                                                  aaCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CAT‑GTCTCCGGCt                                                                                                                                                                                                                                                                            <  7:244468/149‑1 (MQ=255)

                                                                   aCCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CAT‑GTCTCCGGCtt                                                                                                                                                                                                                                                                           <  8:700011/149‑1 (MQ=255)

                                                                     ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTa                                                                                                                                                                                                                                                                         <  6:1186192/149‑1 (MQ=255)

                                                                     ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTa                                                                                                                                                                                                                                                                         >  5:905501/1‑149 (MQ=255)

                                                                     ccTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTa                                                                                                                                                                                                                                                                         <  7:237117/149‑1 (MQ=255)

                                                                         tttACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGt                                                                                                                                                                                                                                                                     >  8:1129914/1‑149 (MQ=255)

                                                                          ttACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTa                                                                                                                                                                                                                                                                    <  7:1311200/149‑1 (MQ=255)

                                                                              ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CAT‑GTCTCCGGCTTTACAGTAtggg                                                                                                                                                                                                                                                                <  8:714875/149‑4 (MQ=255)

                                                                              ccGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CAT‑GTCTCCGGCTTTACAGTAtggg                                                                                                                                                                                                                                                                >  7:714875/1‑146 (MQ=255)

                                                                                  agagTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTgag                                                                                                                                                                                                                                                            <  5:1074264/149‑1 (MQ=255)

                                                                                     gTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCgg                                                                                                                                                                                                                                                                              <  6:346321/128‑1 (MQ=255)

                                                                                     gTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCgg                                                                                                                                                                                                                                                                              >  5:346321/1‑128 (MQ=255)

                                                                                        gATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATaca                                                                                                                                                                                                                                                      <  8:443388/149‑1 (MQ=255)

                                                                                          tGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                                                                                                                                                    >  7:661446/1‑149 (MQ=255)

                                                                                          tGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                                                                                                                                                                                                    >  6:1127457/1‑149 (MQ=255)

                                                                                           gCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACAccc                                                                                                                                                                                                                                                   <  6:999782/149‑1 (MQ=255)

                                                                                             aGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCAt                                                                                                                                                                                                                                                 >  7:612622/1‑149 (MQ=255)

                                                                                              gATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                                                                                                                                                                                                                >  6:21876/1‑149 (MQ=255)

                                                                                              gATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                                                                                                                                                                                                                >  8:818372/1‑149 (MQ=255)

                                                                                                ttGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                              <  8:1223304/149‑1 (MQ=255)

                                                                                                  gCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                                                                                                                                                                                                            <  7:1097744/149‑1 (MQ=255)

                                                                                                   ccAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                                                                                                                                                                                                           >  5:99733/1‑149 (MQ=255)

                                                                                                   ccAGTGGCATAATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                                                                                                                                                                                                           >  7:92624/1‑149 (MQ=255)

                                                                                                            tGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                                                                                                                                                                                                  <  6:14241/149‑1 (MQ=255)

                                                                                                               ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGtt                                                                                                                                                                                                                               >  7:1195965/1‑149 (MQ=255)

                                                                                                                tttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                                                                                                                                                                                                              <  6:1368001/149‑1 (MQ=255)

                                                                                                                  tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaa                                                                                                                                                                                                                            <  8:578268/149‑1 (MQ=255)

                                                                                                                   ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                                                                                                                                                                                                     >  5:1214129/1‑123 (MQ=255)

                                                                                                                      cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                                                                                                                                                                                                        <  7:1006812/149‑1 (MQ=255)

                                                                                                                       aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCg                                                                                                                                                                                                                       >  6:699468/1‑149 (MQ=255)

                                                                                                                        gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                                                                                                                                                                                                      >  6:819151/1‑149 (MQ=255)

                                                                                                                            ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCt                                                                                                                                                                                                                  >  6:427685/1‑149 (MQ=255)

                                                                                                                                   cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGGCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGTCTTACTGGCAAGAACCCGATTATAAAACTAACATGGCAAGCAGAATAAAAAt                                                                             >  2:340475/1‑275 (MQ=255)

                                                                                                                                    ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTAt                                                                                                                                                                                                                                                                   <  8:542179/92‑1 (MQ=255)

                                                                                                                                    ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                              <  7:379652/113‑1 (MQ=255)

                                                                                                                                    ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                                                                                                                                                                                                          >  8:107923/1‑149 (MQ=255)

                                                                                                                                         cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                                                                                                                                     <  6:1316646/149‑1 (MQ=255)

                                                                                                                                         cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                                                                                                                                                                                                     >  5:1147493/1‑149 (MQ=255)

                                                                                                                                          aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAG‑CAT‑GTCTCCGGCt                                                                                                                                                                                                                                                                            <  6:909291/77‑1 (MQ=37)

                                                                                                                                             aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                                                                                                                                                                                                 >  7:1269790/1‑149 (MQ=255)

                                                                                                                                             aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                                                                                                                                                                                                 <  5:216398/149‑1 (MQ=255)

                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                              >  5:1036220/1‑103 (MQ=255)

                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                                                                                                                                                                                                              <  6:1036220/103‑1 (MQ=255)

                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCttttt                                                                                                                                                                                                              >  7:450239/1‑135 (MQ=255)

                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                                                                                                                                >  7:1437277/1‑149 (MQ=255)

                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                                                                                                                                >  5:569300/1‑149 (MQ=255)

                                                                                                                                              aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                                                                                                                                                                                                >  7:1140723/1‑149 (MQ=255)

                                                                                                                                                   aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCa                                                                                                                                                                                           >  5:556623/1‑149 (MQ=255)

                                                                                                                                                   aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTTCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACCGTGAACAACAGGTTGGCTCACTGGCAAGAACCAGATTATAAAACTAAGCTGGCGCGCAGAATAAAAATCAACCAACTTATGGa                                                              >  4:67009/1‑274 (MQ=255)

                                                                                                                                                    ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc                                                                                                                                                                                          >  8:839111/1‑149 (MQ=255)

                                                                                                                                                          tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa                                                                                                                                                                                    >  8:960983/1‑149 (MQ=255)

                                                                                                                                                             cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGc                                                                                                                                                                                                    >  7:32574/1‑130 (MQ=255)

                                                                                                                                                             cATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc                                                                                                                                                                                 >  6:923799/1‑149 (MQ=255)

                                                                                                                                                                 tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATacac                                                                                                                                                                                                                                                     >  7:880833/1‑77 (MQ=255)

                                                                                                                                                                 tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                                                                                                                                                             <  8:612622/149‑1 (MQ=255)

                                                                                                                                                                  gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATc                                              >  2:68563/1‑275 (MQ=255)

                                                                                                                                                                   aGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGc                                                                                                                                                                           <  8:1437277/149‑1 (MQ=255)

                                                                                                                                                                    gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                                                                                                                                                          >  8:232309/1‑149 (MQ=255)

                                                                                                                                                                     cGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAg                                                                                                                                                                                                                                  >  7:1044863/1‑92 (MQ=255)

                                                                                                                                                                       aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg                                                                                                                                                                                        >  4:240544/1‑132 (MQ=255)

                                                                                                                                                                       aTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtc                                                                                                                                                                       <  8:18550/149‑1 (MQ=255)

                                                                                                                                                                           cccGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa                                                                                                                                                                                    <  2:240544/132‑1 (MQ=255)

                                                                                                                                                                            ccGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGtcactca                                                                                                                                                                  <  7:687415/149‑1 (MQ=255)

                                                                                                                                                                                   tCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAAtt                                                                                                                                                           <  8:344757/149‑1 (MQ=255)

                                                                                                                                                                                     ccccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTAt                                                                                                                                                         >  7:352884/1‑149 (MQ=255)

                                                                                                                                                                                     ccccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTAt                                                                                                                                                         >  8:1299288/1‑149 (MQ=255)

                                                                                                                                                                                     ccccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTAt                                                                                                                                                         >  5:48225/1‑149 (MQ=255)

                                                                                                                                                                                      cccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATg                                                                                                                                                        <  5:427685/149‑1 (MQ=255)

                                                                                                                                                                                       ccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg                                                                                                                                                       <  8:1269790/149‑1 (MQ=255)

                                                                                                                                                                                       ccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg                                                                                                                                                       <  8:830881/149‑1 (MQ=255)

                                                                                                                                                                                       ccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg                                                                                                                                                       <  7:839111/149‑1 (MQ=255)

                                                                                                                                                                                       ccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg                                                                                                                                                       <  6:619885/149‑1 (MQ=255)

                                                                                                                                                                                       ccATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgg                                                                                                                                                       >  5:855970/1‑149 (MQ=255)

                                                                                                                                                                                        cATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggg                                                                                                                                                      <  8:1195965/149‑1 (MQ=255)

                                                                                                                                                                                        cATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATggg                                                                                                                                                      >  6:326817/1‑149 (MQ=255)

                                                                                                                                                                                           aCTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGcc                                                                                                                                                                          <  6:727714/126‑1 (MQ=255)

                                                                                                                                                                                            cTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATgggg                                                                                                                                                     >  2:360063/1‑146 (MQ=255)

                                                                                                                                                                                             tGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt                                                                                                                                                                                                                                <  6:1438490/70‑1 (MQ=255)

                                                                                                                                                                                             tGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGt                                                                                                                                                                                                                                >  5:1438490/1‑70 (MQ=255)

                                                                                                                                                                                             tGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa                                                                                                                                                 >  6:1239664/1‑149 (MQ=255)

                                                                                                                                                                                             tGTAAAGCCGGAG‑CAT‑GTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACa                                                                                                                                                 >  7:177178/1‑149 (MQ=255)

                                                                                                                                                                                               tAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGt                                                                                                                                               <  5:326817/149‑1 (MQ=255)

                                                                                                                                                                                               tAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCGATTCg                                                                                                                                                                                              >  1:65483/1‑102 (MQ=255)

                                                                                                                                                                                               tAAAGCCGGAG‑CAT‑GTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGtt                                                                                                                                                                             >  6:1463471/1‑119 (MQ=255)

                                                                                                                                                                                                 aaGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGa                                                                                                                                             >  8:885569/1‑149 (MQ=255)

                                                                                                                                                                                                  aGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa                                                                                                                                            >  7:808997/1‑149 (MQ=255)

                                                                                                                                                                                                  aGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCATTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaa                                                                                                                                            >  8:1050188/1‑149 (MQ=255)

                                                                                                                                                                                                   gCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAATCATAATCGAAGGTTCAATATCGCCCCTTCTTGATCACCAGCAATTCATCACCGAGCAGACGGTTGCCGTCACTCACTAATTTATAGGAGAAAGAGaac                                                                                                                                           >  8:981235/1‑149 (MQ=255)

                                                                                                                                                                                                    ccGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCa            >  4:314256/1‑275 (MQ=255)

                                                                                                                                                                                                     cGGAGACAT‑G‑CTCCGGCTTTACAGTATTCCTGCGAATCCACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTg                                                                   <  4:349/219‑1 (MQ=255)

                                                                                                                                                                                                     cGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAatat                                                                                                                                                                                                                         >  5:716537/1‑69 (MQ=255)

                                                                                                                                                                                                      ggAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGaacaac                                                                                                                                        <  6:100509/149‑1 (MQ=255)

                                                                                                                                                                                                         gaCAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAgg                                                                                                                                     <  6:556623/149‑1 (MQ=255)

                                                                                                                                                                                                           cAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGtt                                                                                                                                   <  7:989335/149‑1 (MQ=255)

                                                                                                                                                                                                            aT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTg                                                                                                                                  >  6:1287534/1‑149 (MQ=255)

                                                                                                                                                                                                             t‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTgg                                                                                                                                 <  5:755428/149‑1 (MQ=255)

                                                                                                                                                                                                               g‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCGCTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGATCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCTGCCAGGCTAACAGCGTTTCTTTGTTGGTCAGGCTaaaaaa  >  2:161860/1‑275 (MQ=255)

GCTGCTGTTTGTTTGATCTCTATCCACTTTTGTATACCTGTATTAAACCGGGGAAATGCTCATCTTAACCCTGTTTACCCGGAGAGTTGATGCAGATTGCCAGTGGCATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAT‑G‑CTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGACGGTTGCCGTCACTCACTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAA  >  NC_000913/4520298‑4520778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`