BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,477,878	T→A	7.6%	intergenic (+20/+29)	slyX → / ← slyD	phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,878	0	T	A	7.6%	481.5 / 24.4	145	intergenic (+20/+29)	slyX/slyD	phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand): ref base T (73/60); new base A (5/6); total (79/66)
Fisher's exact test for biased strand distribution p-value = 7.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 145 total aligned reads displayed.

CGCGGCGACCATTCGACAAAAGAGGTGAATCATGCAGGATTTATCATTGGAAGCACGCCTGGCAGAGCTGGAGAGCCGACTGGCTTTTCAGGAAATCACCATTGAAGAACTGAACGTCACGGTGACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGGTGATCGTGCGCGCCGTGAACGTGACCATGAGCCAGTTCTTCTTCAGTCGCTTCGCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAAC  >  NC_000913/3477609‑3478143

cgcgGCGACCATTCGACAAAAGAGGTGAATCATGCAGGATTTATCATTGGAAGCACGCCTGGCAGAGCTGGAGAGCCGACTGGCTTTTCAGGAAATCACCCTTGAAGAACTGAACGTCACGGTGACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTCAAAGCCAGCCAGCCGTCGAACATCGCGCCGCAGGCTGAAAAAACGCCACCGCCA‑ACTATTGGAGGCGTAAAAAAACCGGGGATAcc                                                                                                                                                                                                                                                                         >  4:88313/1‑273 (MQ=255)

                                         tATCAATGGAAACAAGCCTGGAAGGGGTGGAGAGCGTGCGGGCTTTTAAGGAAATCACCATTGAAAAACTGAACGGCACGGTGACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGGCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccgcaac                                                                                                                                                                                                                               <  4:244625/274‑1 (MQ=255)

                                                                           ccGACTGGCTTTTCAGGAAATCACCATTGAAGAACTGAACGTCACGGTGACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAACAAAGCGGGGAGTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCCACCGACGTTGCCTTTACCGCCACA‑GCCGCCTTcccc                                                                                                                                                                                            >  2:309470/1‑274 (MQ=255)

                                                                                              aTCACCATTGAAGAACTGAACGTCACGGTGACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAACGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAACAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTTGCAACCGCCACCGCCGTTTGCTTTACCGCCACACGCA‑CCCTCGCCACCCTGTTccaggcccggc                                                                                                                                                                         >  4:58322/1‑264 (MQ=255)

                                                                                                                           gACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTc                                                                                                                                                                                                                                                                          <  8:754686/149‑1 (MQ=255)

                                                                                                                              cGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTcccc                                                                                                                                                                                                                                                                       >  7:1275736/1‑149 (MQ=255)

                                                                                                                                 tCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCt                                                                                                                                                                                                                                                                    >  5:571595/1‑149 (MQ=255)

                                                                                                                                  cATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACGTTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCtt                                                                                                                                                                                                                                                                   <  8:1378873/149‑1 (MQ=255)

                                                                                                                                  cATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGAATCCCCGCtt                                                                                                                                                                                                                                                                   <  6:654877/149‑1 (MQ=255)

                                                                                                                                  cATGAAATCGAGATAGCGAAAGTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCACAGGCGGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGAATCCCCGCtt                                                                                                                                                                                                                                                                   <  7:902851/149‑1 (MQ=255)

                                                                                                                                   aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCttt                                                                                                                                                                                                                                                                  <  8:1448421/149‑1 (MQ=255)

                                                                                                                                   aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCttt                                                                                                                                                                                                                                                                  >  8:1140860/1‑149 (MQ=255)

                                                                                                                                   aTGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGAATCCCCGCttt                                                                                                                                                                                                                                                                  <  6:438897/149‑1 (MQ=255)

                                                                                                                                    tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                                                                                                                                                 >  5:255347/1‑149 (MQ=255)

                                                                                                                                    tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                                                                                                                                                 <  7:1417711/149‑1 (MQ=255)

                                                                                                                                    tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                                                                                                                                                 >  5:751819/1‑149 (MQ=255)

                                                                                                                                    tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                                                                                                                                                 >  6:21345/1‑149 (MQ=255)

                                                                                                                                    tGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCtttt                                                                                                                                                                                                                                                                 <  8:706689/149‑1 (MQ=255)

                                                                                                                                        aTGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGt                                                                                                                                                                                                                                                             >  5:1078949/1‑149 (MQ=255)

                                                                                                                                        aTGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGt                                                                                                                                                                                                                                                             <  7:615064/149‑1 (MQ=255)

                                                                                                                                         tGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTc                                                                                                                                                                                                                                                            <  5:1386530/149‑1 (MQ=255)

                                                                                                                                           gagaTGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCCCA‑GCAGCCTTCGCCACCGTGTTCATGACCGTAATCGTGGCCCTGACCGCCCCAGCAACCGTCGTGGTCGTGAGCgagg                                                                                                                            >  2:135310/1‑274 (MQ=255)

                                                                                                                                                 gCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTc                                                                                                                                                                                                                                                    <  5:1129086/149‑1 (MQ=255)

                                                                                                                                                   gAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCTCGCTTTTTTGTCACTTTTTCgg                                                                                                                                                                                                                                                  >  8:1202312/1‑149 (MQ=255)

                                                                                                                                                   gAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCgg                                                                                                                                                                                                                                                  >  6:47899/1‑149 (MQ=255)

                                                                                                                                                     aaCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCGGACAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACAGCCACATTATA‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTATCACTATTTCGGTa                                                                                                                                                                                                                                                >  8:898110/1‑149 (MQ=255)

                                                                                                                                                      aCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                                                                                                                                                <  6:471102/132‑1 (MQ=255)

                                                                                                                                                      aCTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCttttt                                                                                                                                                                                                                                                                >  5:471102/1‑132 (MQ=255)

                                                                                                                                                         tcggctgattcTGCGGCGGCTGCCCGGGAAGTTAAAAGCAGCCCCGCCGTTGAACGTGGCGTGGCAGGCTGAAGAAGCGCCACGGCACCATTATG‑GAGGCGTAAACAAGGCGGGGATTCCCCGTTTTTTTGTCACTTTTTCGGTATTGGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGGTGATCGTgcgc                                                                                                              <  4:135310/265‑1 (MQ=255)

                                                                                                                                                          cgcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                                                                                                                                           <  7:1096422/149‑1 (MQ=255)

                                                                                                                                                          cgcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                                                                                                                                           <  8:386287/149‑1 (MQ=255)

                                                                                                                                                           gcgATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTg                                                                                                                                                                                                                                          <  7:1024202/149‑1 (MQ=255)

                                                                                                                                                             gATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGc                                                                                                                                                                                                                                                                     >  5:1364641/1‑120 (MQ=255)

                                                                                                                                                             gATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgc                                                                                                                                                                                                                                        >  6:527299/1‑149 (MQ=255)

                                                                                                                                                               tcatgTGCGTCGGCTGACCGGGAAGTTAAAAGCCAGCCAGCCGTCGAACATGGCGGCGCAGGCTGAAGAAACGCCACCGCAACATTATT‑GAGGCGTAAAAAAATCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATGAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGGTGATCGTGCGCGCCGTg                                                                                                        <  2:58322/274‑1 (MQ=255)

                                                                                                                                                                  tcTGCGTCTGCTGACCGAGAAGTTAAAAGGCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGgcaaccg                                                                                                                                                                                                                                   >  7:1185613/1‑149 (MQ=255)

                                                                                                                                                                    tGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTAGGTATTAGTGGCAACCGCAACCGACGTTGCCTTTACCGCCACA‑GCCGCCTTCGCCACCGTTTTCATGCCCGTGATCGTTGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGACCGTGGTGGTGATCGTGCGCGCCGTGGACGt                                                                                                   >  2:1806/1‑274 (MQ=255)

                                                                                                                                                                       gTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGAATCCCCGc                                                                                                                                                                                                                                                                     >  8:342984/1‑110 (MQ=255)

                                                                                                                                                                       gTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGAATCCCCGc                                                                                                                                                                                                                                                                     <  7:342984/110‑1 (MQ=255)

                                                                                                                                                                          tgctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAAccgcc                                                                                                                                                                                                                           <  6:989930/149‑1 (MQ=255)

                                                                                                                                                                          tgctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAAccgcc                                                                                                                                                                                                                           <  8:3559/149‑1 (MQ=255)

                                                                                                                                                                           gctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCCGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTCGTGGCAACCGCAACCGCCGTTGCCTTTCCCGCCACA‑GCAGCCTTCGCCCCCGTGTTCCTGACCGTGATCGTGGCCTTGACCGCCGCAGCAACCGCCGTGGGCGTGGTCCTGGCGGTGAACGTGCGCGACGtcacacttccacgt                                                                                            >  4:158384/1‑261 (MQ=255)

                                                                                                                                                                           gctgACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCCTGACCGCCgca                                                                                                                                                     >  2:165219/1‑217 (MQ=255)

                                                                                                                                                                               aCCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGc                                                                                                                                                                                                                      <  6:1117363/149‑1 (MQ=255)

                                                                                                                                                                                 cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCt                                                                                                                                                                                                                    >  7:459270/1‑149 (MQ=255)

                                                                                                                                                                                 cGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCt                                                                                                                                                                                                                    <  6:1076180/149‑1 (MQ=255)

                                                                                                                                                                                  gagaAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCtt                                                                                                                                                                                                                   >  8:1443557/1‑149 (MQ=255)

                                                                                                                                                                                     aaGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTAc                                                                                                                                                                                                                <  5:62107/149‑1 (MQ=255)

                                                                                                                                                                                        ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGc                                                                                                                                                                                                             >  8:899273/1‑149 (MQ=255)

                                                                                                                                                                                        ttAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGc                                                                                                                                                                                                             >  7:507454/1‑149 (MQ=255)

                                                                                                                                                                                          aaaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCca                                                                                                                                                                                                           >  8:242143/1‑149 (MQ=255)

                                                                                                                                                                                           aaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCg                                                                                                                                                                                                                                                   >  5:1341074/1‑108 (MQ=255)

                                                                                                                                                                                           aaaGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCg                                                                                                                                                                                                                                                   <  6:1341074/108‑1 (MQ=255)

                                                                                                                                                                                               ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCAca‑gca                                                                                                                                                                                                     <  6:571595/149‑1 (MQ=255)

                                                                                                                                                                                                 agccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACa‑gcagc                                                                                                                                                                                                   >  5:19730/1‑149 (MQ=255)

                                                                                                                                                                                                  gccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGcc                                                                                                                                                                                                  >  6:605825/1‑149 (MQ=255)

                                                                                                                                                                                                     agccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTc                                                                                                                                                                                               >  6:1267482/1‑149 (MQ=255)

                                                                                                                                                                                                     agccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTc                                                                                                                                                                                               >  8:828992/1‑149 (MQ=255)

                                                                                                                                                                                                        ctgcGAACACGGCGTCGCAGGCGGAAGAAACGCCACCGCCACATTATG‑TGGGGGTAAAAAAAGCGGGGATCCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGGTGATCGTGCGCGCCGTGAACGTGACCATGAGCCAGTTCTTCTTCAGTCGCTTCGCGAAt                                                              <  4:309470/272‑1 (MQ=255)

                                                                                                                                                                                                          tCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCAc                                                                                                                                                                                          >  5:467030/1‑149 (MQ=255)

                                                                                                                                                                                                             aaCATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCgt                                                                                                                                                                                       <  5:527299/149‑1 (MQ=255)

                                                                                                                                                                                                                  cgcgTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCa                                                                                                                                                                                  <  5:1094970/149‑1 (MQ=255)

                                                                                                                                                                                                                           ggCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGa                                                                                                                                                                         <  6:721436/149‑1 (MQ=255)

                                                                                                                                                                                                                              tGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCg                                                                                                                                                                      >  6:212350/1‑149 (MQ=255)

                                                                                                                                                                                                                                aagaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                                                                                                                                           >  6:1432896/1‑79 (MQ=255)

                                                                                                                                                                                                                                aagaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGt                                                                                                                                                                                                                                           <  5:1432896/79‑1 (MQ=255)

                                                                                                                                                                                                                                aagaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTg                                                                                                                                                                    >  5:49315/1‑149 (MQ=255)

                                                                                                                                                                                                                                aagaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTg                                                                                                                                                                    <  5:249617/149‑1 (MQ=255)

                                                                                                                                                                                                                                aagaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTg                                                                                                                                                                    >  7:492204/1‑149 (MQ=255)

                                                                                                                                                                                                                                 agaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTgg                                                                                                                                                                   >  8:619681/1‑149 (MQ=255)

                                                                                                                                                                                                                                  gaaACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCTCCGTGTTCATGACCGTGATCGTGGc                                                                                                                                                                  >  7:559395/1‑149 (MQ=255)

                                                                                                                                                                                                                                    aaCGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTTCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCa                                                                                                                                                                >  8:248214/1‑149 (MQ=255)

                                                                                                                                                                                                                                    aaCGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCa                                                                                                                                                                <  8:1361454/149‑1 (MQ=255)

                                                                                                                                                                                                                                     aCGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCAt                                                                                                                                                               >  7:349743/1‑149 (MQ=255)

                                                                                                                                                                                                                                       gccaccgccacATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGa                                                                                                                                                             <  8:694279/149‑1 (MQ=255)

                                                                                                                                                                                                                                          accgccacATTATT‑GAGGCGTAAAAAAAGCGGGGATTccc                                                                                                                                                                                                                                                                        <  6:693122/40‑1 (MQ=255)

                                                                                                                                                                                                                                              ccacATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACcgccgc                                                                                                                                                      >  6:661006/1‑149 (MQ=255)

                                                                                                                                                                                                                                                  attatt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTc                                                                                                                                                                                               >  7:1458054/1‑104 (MQ=255)

                                                                                                                                                                                                                                                  attatt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTc                                                                                                                                                                                               <  8:1458054/104‑1 (MQ=255)

                                                                                                                                                                                                                                                  attatt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgcagca                                                                                                                                                  >  7:1338294/1‑149 (MQ=255)

                                                                                                                                                                                                                                                  attatt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCgcagca                                                                                                                                                  >  7:723638/1‑149 (MQ=255)

                                                                                                                                                                                                                                                   ttatt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCaa                                                                                                                                                 <  5:483483/149‑1 (MQ=255)

                                                                                                                                                                                                                                                    tatt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAAc                                                                                                                                                <  6:255347/149‑1 (MQ=255)

                                                                                                                                                                                                                                                      tt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcg                                                                                                                                              >  7:157058/1‑149 (MQ=255)

                                                                                                                                                                                                                                                      tt‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcg                                                                                                                                              >  7:753718/1‑149 (MQ=255)

                                                                                                                                                                                                                                                          aGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGGTGATCGTGCGCGCCGTGAACGTGACCATGAGCCAGTTCTTCTTCAGTCGCTTCGCGAATCGCCCCAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCAttgg              >  2:40793/1‑271 (MQ=255)

                                                                                                                                                                                                                                                            gCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAAcc                                                                                                                                               >  2:301881/1‑143 (MQ=255)

                                                                                                                                                                                                                                                                aaaaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACcgtcg                                                                                                                                           <  4:301881/143‑1 (MQ=255)

                                                                                                                                                                                                                                                                 aaaaaaGCGGGTAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCACCGCAGCAACCgtcgtggtcgt                                                                                                                                    >  7:826875/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                 aaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcg                                                                                                                                     <  6:830323/148‑1 (MQ=255)

                                                                                                                                                                                                                                                                 aaaaaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggtcg                                                                                                                                     >  5:830323/1‑148 (MQ=255)

                                                                                                                                                                                                                                                                  aaaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACTGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGACCGTGGCCATGACCTCCGCAGCACCCgtcgtggtcgtg                                                                                                                                   >  6:858501/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   aaaaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGa                                                                                                                                  >  7:759018/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                    aaaGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGAt                                                                                                                                 >  6:1030889/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                     aaGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATc                                                                                                                                <  8:559395/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                       gCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgt                                                                                                                              >  5:952885/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                        cGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtg                                                                                                                             <  7:684732/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                          gggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggt                                                                                                                           <  5:1153119/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                          gggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggt                                                                                                                           <  8:1275736/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                           ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCgtggtg                                                                                                                          >  8:1434425/1‑149 (MQ=255)

CGCGGCGACCATTCGACAAAAGAGGTGAATCATGCAGGATTTATCATTGGAAGCACGCCTGGCAGAGCTGGAGAGCCGACTGGCTTTTCAGGAAATCACCATTGAAGAACTGAACGTCACGGTGACCGCTCATGAAATGGAGATGGCGAAACTGCGCGATCATCTGCGTCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATT‑GAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACA‑GCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGTCGTGATCGTGGTGGTGATCGTGCGCGCCGTGAACGTGACCATGAGCCAGTTCTTCTTCAGTCGCTTCGCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAAC  >  NC_000913/3477609‑3478143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`