BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,018,983	A→T	6.8%	intergenic (+46/+30)	matP → / ← ompA	Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,983	0	A	T	6.8%	658.6 / 30.5	192	intergenic (+46/+30)	matP/ompA	Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
Reads supporting (aligned to +/- strand): ref base A (100/79); new base T (5/8); total (105/87)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-03

Only 100 of 200 total aligned reads displayed.

ATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTA  >  NC_000913/1018732‑1019249

                             tcgatcgaTCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                                                                                                                                                                                                                         <  2:237355/274‑1 (MQ=255)

                                               aTTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGttttt                                                                                                                                                                                                                                                              >  2:334554/1‑219 (MQ=255)

                                               aTTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTa                                                                                                                                                                                                       >  2:161222/1‑274 (MQ=255)

                                                                             cAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCa                                                                                                                                                                        <  2:53031/275‑1 (MQ=255)

                                                                                cttagcgtgaaaaCGCTTATGGAAAGGATAGGTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTATTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAgg                                                                                                                                                                      <  4:260144/265‑1 (MQ=255)

                                                                                       gTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGAt                                                                                                                                                              <  4:67564/275‑1 (MQ=255)

                                                                                                  tCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGc                                                                                                                                                                                                                                     >  4:290207/1‑193 (MQ=255)

                                                                                                    tGAAACGATTGTTGTGCTGATTGAAGAGGCGGAAAAAAAAGAGAAATACGCGAAAAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACg                                                                                                                                                  <  4:114905/274‑1 (MQ=255)

                                                                                                          gATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCgcagca                                                                                                                                                                                                                                                                         >  5:524317/1‑149 (MQ=255)

                                                                                                                  aGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGtt                                                                                                                                                                                                                                                                 <  6:602710/149‑1 (MQ=255)

                                                                                                                  aGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGtt                                                                                                                                                                                                                                                                 <  6:872243/149‑1 (MQ=255)

                                                                                                                  aGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGtt                                                                                                                                                                                                                                                                 <  5:896311/149‑1 (MQ=255)

                                                                                                                  aGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGtt                                                                                                                                                                                                                                                                 <  6:1186674/149‑1 (MQ=255)

                                                                                                                  aGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGtt                                                                                                                                                                                                                                                                 >  7:953180/1‑149 (MQ=255)

                                                                                                                    cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGtttt                                                                                                                                                                                                                                                               >  6:1427518/1‑149 (MQ=255)

                                                                                                                    cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgcag                                                                                                                                                      >  2:29434/1‑254 (MQ=255)

                                                                                                                     tGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAGTTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGttttt                                                                                                                                                                                                                                                              <  7:801073/149‑1 (MQ=255)

                                                                                                                      gATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGtttttt                                                                                                                                                                                                                                                             <  5:349988/149‑2 (MQ=255)

                                                                                                                       aTTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCt                                                                                                                                                                                                                                                            >  6:154926/1‑149 (MQ=255)

                                                                                                                            aGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAg                                                                                                                                                                                                                                                       >  8:14522/1‑149 (MQ=255)

                                                                                                                             gATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGa                                                                                                                                                                                                                                                      >  5:1393298/1‑149 (MQ=255)

                                                                                                                                  ggAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTATTTCTACCAGACgaga                                                                                                                                                                                                                                                 >  5:1348482/1‑149 (MQ=255)

                                                                                                                                   gAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGGAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGaa                                                                                                                                                                                                                                                <  5:303827/149‑1 (MQ=255)

                                                                                                                                   gAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGaa                                                                                                                                                                                                                                                <  6:1348482/149‑1 (MQ=255)

                                                                                                                                   gAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGaa                                                                                                                                                                                                                                                >  7:538181/1‑149 (MQ=255)

                                                                                                                                       aCAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTa                                                                                                                                                                                                                                            >  7:1138934/1‑149 (MQ=255)

                                                                                                                                          aaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCgg                                                                                                                                                                                                                                                                     >  5:815385/1‑121 (MQ=255)

                                                                                                                                          aaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGc                                                                                                                                                                                                                                         <  6:636277/149‑1 (MQ=255)

                                                                                                                                           agagAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGcc                                                                                                                                                                                                                                        >  8:939361/1‑149 (MQ=255)

                                                                                                                                            gagaAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGttt                                                                                                                                                                                                                                                                <  7:588917/124‑1 (MQ=255)

                                                                                                                                                  tACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGTTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCt                                                                                                                                                                                                                                 <  5:154926/149‑1 (MQ=255)

                                                                                                                                                   aCGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTg                                                                                                                                                                                                                                >  7:5272/1‑149 (MQ=255)

                                                                                                                                                    cgcgAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa                                                                                                                                                                                                                               <  8:864414/149‑1 (MQ=255)

                                                                                                                                                       gAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAGCTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGtt                                                                                                                                                                                                                            >  6:106664/1‑149 (MQ=255)

                                                                                                                                                       gAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGtt                                                                                                                                                                                                                            >  8:809508/1‑149 (MQ=255)

                                                                                                                                                         aTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGttttt                                                                                                                                                                                                                                                              <  7:852359/113‑1 (MQ=255)

                                                                                                                                                         aTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCTGCTGCGGGGttttt                                                                                                                                                                                                                                                              >  8:852359/1‑113 (MQ=255)

                                                                                                                                                             aaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCggg                                                                                                                                                                                                                                                                    >  5:208873/1‑103 (MQ=255)

                                                                                                                                                                   ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTc                                                                                                                                                                                                                                                             <  7:351496/104‑1 (MQ=255)

                                                                                                                                                                    tctcTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGa                                                                                                                                                                                                               <  7:456817/149‑1 (MQ=255)

                                                                                                                                                                     ctctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTAACGTTGTCACAGGTTTGGCCAGTAACCGGGTTGGATTCGCCCATACCAGGTCCGGAGAt                                                                                >  4:237813/1‑275 (MQ=255)

                                                                                                                                                                      tctGAAGCCGGATCTGCAGGCATTGCGGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAACGGC‑CAAAAAAACCCCGCAGCAGCGGGGAGTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATa                                                                                                                                                                                                             >  7:1287572/1‑149 (MQ=255)

                                                                                                                                                                      tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCt                                                                                                                                                                                                                                       >  6:386123/1‑123 (MQ=255)

                                                                                                                                                                      tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATa                                                                                                                                                                                                             >  6:1391586/1‑149 (MQ=255)

                                                                                                                                                                             cAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTaa                                                                                                                                                                                                      <  7:1368980/149‑1 (MQ=255)

                                                                                                                                                                               ggATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACt                                                                                                                                                                                                    >  8:785427/1‑149 (MQ=255)

                                                                                                                                                                               ggATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGcc                                                                      >  2:255798/1‑275 (MQ=255)

                                                                                                                                                                                gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACtt                                                                                                                                                                                                   >  6:708534/1‑149 (MQ=255)

                                                                                                                                                                                gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGcgacga                                                                                                                                                                                  >  4:322485/1‑166 (MQ=255)

                                                                                                                                                                                    tGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCg                                                                                                                                                                              <  2:322485/166‑1 (MQ=255)

                                                                                                                                                                                        ggCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgcag                                                                                                                                                      >  2:2281/1‑186 (MQ=255)

                                                                                                                                                                                         gCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCgg                                                                                                                                                                                                                                                                     <  8:5301/74‑1 (MQ=255)

                                                                                                                                                                                            ttGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACg                                                                                                                                                  <  4:2281/186‑1 (MQ=255)

                                                                                                                                                                                                   gTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATc                                                                                                                                                                                <  7:785427/149‑1 (MQ=255)

                                                                                                                                                                                                     aaGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCg                                                                                                                                                                              <  8:813256/149‑1 (MQ=255)

                                                                                                                                                                                                       ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGa                                                                                                                                                                            <  6:524317/149‑1 (MQ=255)

                                                                                                                                                                                                       ggAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTaa                                              >  2:76422/1‑275 (MQ=255)

                                                                                                                                                                                                        gAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTaa                                                                                                                                                                                                      <  6:1250030/122‑1 (MQ=255)

                                                                                                                                                                                                        gAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAg                                                                                                                                                                           >  8:577290/1‑149 (MQ=255)

                                                                                                                                                                                                         aaTAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGc                                                                                                                                                                          >  6:41831/1‑149 (MQ=255)

                                                                                                                                                                                                            aaCTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCCCAGGTGTTGCCAGTAACCGGGtt                                                                                                           >  4:158599/1‑210 (MQ=255)

                                                                                                                                                                                                               tGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCa                                                                                                                                                                    >  8:711042/1‑149 (MQ=255)

                                                                                                                                                                                                                 aCGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGt                                                                                                                                                                  >  6:117279/1‑149 (MQ=255)

                                                                                                                                                                                                                   gAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATCCCACGTGCGGAGATCTTGTCTGcc                                                                      >  4:47935/1‑239 (MQ=255)

                                                                                                                                                                                                                   gAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGACAACTTAAGCATGCGGCTGAGTTACAACGTCTTTGATCCCTTTAACTTCGATCTCTACGCGACGATCCGGTGCCAGGCAGTcc                                                                                                                                                                >  8:12647/1‑148 (MQ=255)

                                                                                                                                                                                                                       gTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCa                                                                                                                                                            <  6:1262530/149‑1 (MQ=255)

                                                                                                                                                                                                                       gTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCa                                                                                                                                                            >  7:1438103/1‑149 (MQ=255)

                                                                                                                                                                                                                            ttCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgca                                                                                                                                                       >  6:996773/1‑149 (MQ=255)

                                                                                                                                                                                                                            ttCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGAGGCTGa                                                                                                                                                                                                                               >  6:536288/1‑77 (MQ=255)

                                                                                                                                                                                                                            ttCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGAGGCTGa                                                                                                                                                                                                                               <  5:536288/77‑1 (MQ=255)

                                                                                                                                                                                                                               aaTTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGt                                                                                                                                                          <  6:815740/143‑1 (MQ=255)

                                                                                                                                                                                                                               aaTTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgcagca                                                                                                                                                    >  8:495389/1‑149 (MQ=255)

                                                                                                                                                                                                                                aTTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCAc                                                                                                                                                   >  6:260995/1‑149 (MQ=255)

                                                                                                                                                                                                                                    aCTAAAGGC‑AAAAAAAGCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTAAAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGa                 <  2:128394/275‑1 (MQ=255)

                                                                                                                                                                                                                                      tAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGtt                                                                                                                                             >  7:734777/1‑149 (MQ=255)

                                                                                                                                                                                                                                      tAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGtt                                                                                                                                             >  5:1093730/1‑149 (MQ=255)

                                                                                                                                                                                                                                       aaaGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTc                                                                                                                                                                 >  7:877887/1‑128 (MQ=255)

                                                                                                                                                                                                                                       aaaGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGttt                                                                                                                                            >  8:767401/1‑149 (MQ=255)

                                                                                                                                                                                                                                        aaagC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTc                                                                                                                                           <  5:663725/146‑1 (MQ=255)

                                                                                                                                                                                                                                        aaGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTc                                                                                                                                           <  7:578193/149‑1 (MQ=255)

                                                                                                                                                                                                                                         aGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTa                                                                                                                                                                                          >  6:1435377/1‑101 (MQ=255)

                                                                                                                                                                                                                                          ggC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAg                                                                                                                                                                       >  7:254665/1‑119 (MQ=255)

                                                                                                                                                                                                                                          ggC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAg                                                                                                                                                                       <  8:254665/119‑1 (MQ=255)

                                                                                                                                                                                                                                          ggC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCAc                                                                                                                                         >  5:683121/1‑149 (MQ=255)

                                                                                                                                                                                                                                              aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAGCTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGtt                                                                                                                                      >  7:1106812/1‑149 (MQ=255)

                                                                                                                                                                                                                                              aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGtt                                                                                                                                      >  5:1072741/1‑149 (MQ=255)

                                                                                                                                                                                                                                                aaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGt                                                                                                                                    <  7:1232366/149‑1 (MQ=255)

                                                                                                                                                                                                                                                aaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGGTCTTGTCTGcc                                                                      >  4:218884/1‑211 (MQ=255)

                                                                                                                                                                                                                                                aaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAACTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTc                                                                                                                                           >  1:25597/1‑142 (MQ=255)

                                                                                                                                                                                                                                                   aaaCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcac                                                                                                                                 >  5:420143/1‑149 (MQ=255)

                                                                                                                                                                                                                                                   aaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcac                                                                                                                                 <  5:1237714/149‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcac                                                                                                                                 >  8:685106/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    aaCNCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGt                                                                                                                                       <  3:25597/142‑1 (MQ=255)

                                                                                                                                                                                                                                                    aaCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcaca                                                                                                                                >  5:581927/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    aaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcaca                                                                                                                                >  8:1128093/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    aaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGa                                                                  <  2:218884/211‑1 (MQ=255)

                                                                                                                                                                                                                                                    aaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa  <  4:76422/275‑1 (MQ=255)

                                                                                                                                                                                                                                                     aCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCAc                                                                                                                                         <  5:1261369/139‑1 (MQ=255)

                                                                                                                                                                                                                                                       cccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGgt                                                                                                                             >  8:392750/1‑149 (MQ=255)

                                                                                                                                                                                                                                                        ccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTGTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGgtg                                                                                                                            >  7:842790/1‑149 (MQ=255)

ATGCGGAACACCAGCATACGCGCAAAAAATCGATCGATCTGGAATTTATTGTCTGGCAACGTCTGGCTGGTCTTGCGCAGCGTCGCGGTAAAACGCTTTCTGAAACGATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGC‑AAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTA  >  NC_000913/1018732‑1019249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`