BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,145	C→A	R665S (CGT→AGT)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,145	0	C	A	96.0%	387.0 / NA	129	R665S (CGT→AGT)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base C (0/3); major base A (68/53); minor base G (2/3); total (70/59)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

ATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGC  >  NC_000913/3992873‑3993414

aTTTCCGTTAGCTGGAAGTTTTCCGCTTTGGCGGGAACTAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACCCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                             <  4:178881/275‑1 (MQ=255)

 tttCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                             <  2:84837/274‑1 (MQ=255)

 tttCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGGCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGt                                                                                                                                                                                                                                                                             >  1:458147/1‑274 (MQ=255)

         aGCTGGATGTCGTCAGCTTCGACGATAATCAAAATTTCATGTGAGGTCGCGTTGACATGCTGCAGCGCAACTCGTGGAACGAAG‑TGCGGACGCTGCACTTCAACGGCCAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAANTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCa                                                                                                                                                                                                                                                                   <  4:390810/276‑1 (MQ=255)

           cTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCAcc                                                                                                                                                                                                                                                                 <  2:350350/276‑1 (MQ=255)

               aTGTCTTCAGCTTTTGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAACTGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CACCTGTTTTCTGTGTGTCTTGAATTGGTTCTTTCCAGCACCCGct                                                                                                                                                                                                                                                             >  2:617046/1‑275 (MQ=255)

                          gttGGAGAGAAGCAAAAATGCGTGGCAGGTAGAGGTAACCGGACGTAACGCAAAACGTGGAACGAAG‑TGCGTAAGCTGCACGTCAACGACCAGAAATCGATGGTCGAAGACCTGAAAACTCTTCTCGGCAAAATGCAGCAGGACGCCGCACCGCCAGATAGCGTGGAAGGCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCggg                                                                                                                                                                                                                                                  <  4:358274/275‑1 (MQ=255)

                                              ccTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCCCCCGCCCGGAACCCGGGCGTTTCCAGGCGCTGggcg                                                                                                                                                                                                                               >  4:431934/1‑275 (MQ=255)

                                                     ggtagCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                       <  2:607350/276‑1 (MQ=255)

                                                      ttcggctgtgccTACTTTACCCAAACTCGGTAAACGAAGTTGCG‑ACGCTGTACTTCAACGGTGATCAATCGATGAGCGAAGCCCTGAAAACTATTCTCTGCAAAAGGCATCCGGACGCCGCACCGCCAAATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGGATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                       <  3:458147/265‑1 (MQ=255)

                                                        atcttttaCCTTCTGTACCGCCACTCCTGGAACGAAG‑TGCGTACGCTTCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACCCCGCCCCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCTGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCAGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCa                                                                                                                                                                                                                     <  3:245168/268‑1 (MQ=255)

                                                        agCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCCACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATACTCGGCGAACTGCAGCAGGACGCAGCCCAGCAAGCGAGGGTGGGAGACTTCTGTTATAGCTAGCATCTCCGCGACCTAATTCGGACGAGCACACAG‑CAACTGGTCTCTGACTGTATTGAATTGAGTCTTTCCAGCTAACGCAAGGACCACGGGGGTAACAATGCGGTGCGCGgttgggtgtca                                                                                                                                                                                                                    >  4:495744/1‑268 (MQ=255)

                                                            ttGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAAcc                                                                                                                                                                                                                 >  2:412057/1‑275 (MQ=255)

                                                               aCCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTgg                                                                                                                                                                                                              >  2:531160/1‑275 (MQ=255)

                                                               aCCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGGCTTGAATTGAGTCTTTCACGCACCCGCCAGGAAACCAGGCGTTTCAAGGCGTGGCGCGTGTATGGTaaacaagggg                                                                                                                                                                                                             >  4:620912/1‑269 (MQ=255)

                                                                ccTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGGCTTCTGTTATAGCCAGCATCTGCGCGGCCTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGGTTCAAGGCGCTGCGAGGTTCTGGTCAAACATgg                                                                                                                                                                                                              >  1:487452/1‑274 (MQ=255)

                                                                    ctgTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCGGCGCGTTTCTGGTCAAACCTGGGGgtt                                                                                                                                                                                                         >  2:646782/1‑275 (MQ=255)

                                                                         ccGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTACTTCGTACTCGCTTGCAG‑CACCTGGTTTCTGAGTGTATTGAATTGATTCTTTCCAGCACCCGCCCGGAAACCGGGCGTTGCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGTTTGgtctt                                                                                                                                                                                                   >  4:130175/1‑276 (MQ=255)

                                                                         ccGCAACTCGTGGAACGAAG‑TGCGTACGCTCCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCCCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGCGTGTATTGAATTGATTCTTTCCAGCACCCGCCCGGAAACCGGGCGTTTCAAGGCCCTGCGCGGTTCTGGTCAAACCTGGGGGTTGttct                                                                                                                                                                                                    >  3:221441/1‑275 (MQ=255)

                                                                            cAAGTCGGGGAACGAAG‑GCCGTACGCTGCACTTCAACAGGGAGCAAACGATGGTCGAAGCCCTGGAAACTATTCTCGGCAAATTGCATCAGTACGCCGCACCGCCAGAGAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGa                                                                                                                                                                                                <  4:655383/276‑1 (MQ=255)

                                                                             aaCTCGGGGAACGAAG‑TGCGAACGCTGCACTGCAACGGAGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGAAAAATGCATCAGGACGCCGCACCGCCAGATCGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGaa                                                                                                                                                                                               <  2:31232/276‑1 (MQ=255)

                                                                              aCTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGaa                                                                                                                                                                                               >  3:310155/1‑275 (MQ=255)

                                                                              aCTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGa                                                                                                                                                                                                <  1:175704/274‑1 (MQ=255)

                                                                                     gAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGaa                                                                                                                                                                                       <  2:71250/276‑1 (MQ=255)

                                                                                      aaCGAAG‑TGCGTACGATGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGTCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGAAAGTCTTCTGGTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGaa                                                                                                                                                                                       <  1:432686/275‑1 (MQ=255)

                                                                                          aaG‑TGCGTACGCTGCACTTCAAAGGCGAGCAATCGATGATCGAAGCCCGGAAAAATATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTAt                                                                                                                                                                                  <  2:95009/276‑1 (MQ=255)

                                                                                           aG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATc                                                                                                                                                                                 <  2:245494/276‑1 (MQ=255)

                                                                                            ggggCGTACGCTGGACTTCAACGGCGAGCAATCGATGATGGAAGCCCTGAAAACGATTATCGGCAAAATGCATCAGTACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACACGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTAt                                                                                                                                                                                  <  3:472196/272‑1 (MQ=255)

                                                                                            agttcgagagcggCACTTGAAATGCGAGCAATCGATGATCGAAGCCCTTAAAACTTTTCGCGGCCAAAGGCATCAGGAGGCCGAACCGCCAGGTAGCGTGGAAGTCGTCTGTGAATGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCGGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTAt                                                                                                                                                                                  <  3:337876/263‑1 (MQ=255)

                                                                                            agggCGTACGCATCACTGCAAAGGCGAGCAATCGATAGTCGAACCCCGGAAACCGATTCTCGGCAAAATGCATCAGGACGCCGACCCGCCAGATAGCGTTGAATTCTTCTGTTATAGCCCGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATc                                                                                                                                                                                 <  4:531160/273‑1 (MQ=255)

                                                                                                cGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGttctt                                                                                                                                                                                                   >  4:597546/1‑254 (MQ=255)

                                                                                                 gTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGACGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAGGGCGCTGCGCGGTTCTGGTAAAACCTGGGGGTTGGTCTTCCAGCGCCTGCGTGTATCGGtc                                                                                                                                                                             >  4:609121/1‑274 (MQ=255)

                                                                                                   aCGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGa                                                                                                                                                                                                <  2:597546/254‑1 (MQ=255)

                                                                                                        gCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCCCGTTTCTGTTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAAt                                                                                                                                                                                      >  1:472196/1‑259 (MQ=255)

                                                                                                        gCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCGGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCGTCGAACGCCGGAAGGTATCGGTACAGAAAc                                                                                                                                                                      >  3:312071/1‑275 (MQ=255)

                                                                                                          aCTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTACTTCGTACCCGCGTGCAG‑CAACTGGTTTCTGCGTGTATTGAATTGAGTCTTTCCAGCACCCGACAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTg                                                                                                                                                                    >  3:320677/1‑275 (MQ=255)

                                                                                                              cAACGTCTAGCAATCAATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCCTCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGaaaa                                                                                                                                                               <  2:630538/276‑1 (MQ=255)

                                                                                                                 cGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACg                                                                                                                                                             >  1:388829/1‑275 (MQ=255)

                                                                                                                      aGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAT‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCTGCACCCGCCAGGCAACCGGGCGTTTCACGGCGCTGCGCGTTTCTGGTCCACCCTGGGGGTTGTTCTTCGACCCGCTGATCGTATCGGTCCAGTAACTGGAAAACCCCAt                                                                                                                                                         >  3:555036/1‑274 (MQ=255)

                                                                                                                      aGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGt                                                                                                                                                                                                                       >  4:485707/1‑212 (MQ=255)

                                                                                                                         aaTCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATCGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCaa                                                                                                                                                                                                                    <  2:485707/212‑1 (MQ=255)

                                                                                                                                  tcgacacccTGAAACATATTCTCGGCAAAATGCATCAGGACGCCGGACCACCAGATAGCGGGGAACTCTTCTTTTATAGCCAGCATATGCGCGGCTTAATGCCTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGGATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGgcg                                                                                                                                           <  4:103569/270‑1 (MQ=255)

                                                                                                                                     aaGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAACCGCCATCGAGTTTTATGTcgcg                                                                                                                                         >  1:320095/1‑275 (MQ=255)

                                                                                                                                            gAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATAGAGTTTTATGGCGCGATTTCgta                                                                                                                                 >  2:696162/1‑274 (MQ=255)

                                                                                                                                                 cTATTCTCGGCAAAATGCCTCAGGACGCCTCACCGCCAGTTAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACa                                                                                                                            <  2:620912/276‑1 (MQ=255)

                                                                                                                                                  tATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCAt                                                                                                                                >  1:477982/1‑271 (MQ=255)

                                                                                                                                                        gagCAAAATCGATCAGGACGCGGTACGTCCAGATAGCGTGGAAGTTTTCTTTTATAGCCAGCATCGGCGCGTCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCa                                                                                                                     <  4:412057/274‑1 (MQ=255)

                                                                                                                                                         ggCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATg                                                                                                                                                                                     >  4:598933/1‑211 (MQ=255)

                                                                                                                                                         ggCAAAAGGCATCAGGAAGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTAAGGCCAGCATCTGCGCGGCTTAATTCGTACGCGCGTGCCG‑CAACGGGTTTCTGAGTGTATTGAATTGAGTCTTCCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTGTATGGCGCGATTTCGCATAACAAACTGCa                                                                                                                     <  1:555036/275‑1 (MQ=255)

                                                                                                                                                              aaTGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGcc                                                                                                                <  1:310155/275‑1 (MQ=255)

                                                                                                                                                                       ggACGCCGCACGGCCGGATAGCGGGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAAATGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGc                                                                                                       <  1:255565/275‑1 (MQ=255)

                                                                                                                                                                               ccccGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTANTCAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGTTCAAACCTGTGGGTTGTTCTTCGAACGCCTGAATGTATATGTACATAAACTGAAAAACGCCATCCAGTTTTCTTGAGCGATTTCGCTTTACAAACCACAAGCCCTGACACTGCAGGttcacc                                                                                              >  4:393986/3‑272 (MQ=255)

                                                                                                                                                                                  cGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGGTTTCGCATAACAAACTGACCGGCCTGTTAGTGcctgtctcttgt                                                                                            >  4:410951/1‑264 (MQ=255)

                                                                                                                                                                                     cAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACgg                                                                                                                  <  2:410951/250‑1 (MQ=255)

                                                                                                                                                                                            cGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCaa                                                                                 >  2:264809/1‑276 (MQ=255)

                                                                                                                                                                                            cGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCa                                                                                  >  3:38778/1‑275 (MQ=255)

                                                                                                                                                                                              tGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAACCCTGGGGGTTGTTCTTCGAAAGCCTGCATGTATCGGGACAGAAACTGGACAACGCAATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGAATGGCATGTCAGTGCCAGTTGaaacaaaacagtctacat                                                                               >  4:490942/1‑268 (MQ=255)

                                                                                                                                                                                              tGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAAt                                                                               >  2:230825/1‑276 (MQ=255)

                                                                                                                                                                                               gggaGTCTTCTGTAGTAGCCAGCATCGGCGCGGCTTAATTCGTACTCGCGGGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAAtt                                                                              <  2:507109/273‑1 (MQ=255)

                                                                                                                                                                                                gAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCa                                                                                  <  1:38778/271‑1 (MQ=255)

                                                                                                                                                                                                 aaGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAAGAAACTGCACGGCCTGTCAGTGCGGGTGGAAACAAATCACGTCAAAtt                                                                              >  1:51532/1‑274 (MQ=255)

                                                                                                                                                                                                      ttctGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAAGTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGGCAAAATACcggc                                                                        >  1:620740/1‑275 (MQ=255)

                                                                                                                                                                                                      gtctGTTATAGCCAGCATCTTCGTGGCTTAATTCGTACTCGCTTGCAG‑CAACTTGTTTCTGTGTGTATTGAATTGAGGCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTAGGGGTGGTTCTTCGAACGCCTGAAGGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACcgg                                                                         <  2:619056/273‑1 (MQ=255)

                                                                                                                                                                                                              taGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACg                                                                                                                                                                                             >  2:116296/1‑150 (MQ=255)

                                                                                                                                                                                                               gagccgCATCTGGGCGGCTTAATTCGTATTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGGCTTTCCAGCACCCGCCAGGAAACCGTGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTATTCTTCGAACGCCTGAATGTATCCGTACAGAAACTGGAAAACGCCATCGAGTTTGATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGGGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGtggtgg                                                                <  3:620740/269‑1 (MQ=255)

                                                                                                                                                                                                                gCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGAc                                                              >  1:191797/1‑275 (MQ=255)

                                                                                                                                                                                                                gCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGGGGTGGACg                                                             >  2:212191/1‑276 (MQ=255)

                                                                                                                                                                                                                 ccAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCt                                                                                                                                                                                          <  4:116296/150‑1 (MQ=255)

                                                                                                                                                                                                                   aGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTTAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGAAGGCt                                                          >  2:454442/1‑276 (MQ=255)

                                                                                                                                                                                                                     cATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATCACAAACTGCACGGCCTGTCAGTGCAGGTTGAAGCCAATCACCTCAAATTACCGCCGGGGGATCACGGCttg                                                        >  2:115066/1‑275 (MQ=255)

                                                                                                                                                                                                                     cATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTTAAATTACCGGGGGTGGGGGACGGCtt                                                         >  3:201006/1‑275 (MQ=255)

                                                                                                                                                                                                                     cATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCtt                                                         >  1:326025/1‑275 (MQ=255)

                                                                                                                                                                                                                     cATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGGCAAATTACCGGCGGTGGTGGTCGGCttt                                                        >  2:630984/1‑276 (MQ=255)

                                                                                                                                                                                                                     cATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGGTCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGCGGGCttt                                                        >  4:204364/1‑276 (MQ=255)

                                                                                                                                                                                                                     cATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCAATCGAGTTTTATGGCGCGATTTCGCCTAAAAAACTGCCCTGCCTGTCAGTGCAGGTTGACACACATCACGTCAAATTACCGGCGGTGGTTGACTGCtt                                                         >  2:207032/1‑275 (MQ=255)

                                                                                                                                                                                                                        gggacaggattAATTCGGAGTCGCGTGCAG‑CAACGGGTTTCTGAGGGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGc                                                      <  3:198672/266‑1 (MQ=255)

                                                                                                                                                                                                                        cTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGCTTCAAGGCGCTGCGCGTTTCTGGTCCAACCGGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGGATTACAAAATGCAAGGCCTGTCAGAGCAGGTTGAAACCAATCACGTCAAATTTCCGGCGGGGGTGGACGGCGTTga                                                      >  3:557068/1‑274 (MQ=255)

                                                                                                                                                                                                                              gggttAATTCGGACTAGCG‑GCAGACAACGTGTTTGTAGGGGTTTTGAAGTGGTTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGTGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGa                                                <  3:51532/272‑1 (MQ=255)

                                                                                                                                                                                                                              ggCTTAAGTAGTACTCGCGTGCAG‑CAACTGGTTTCTGATTGGATTGGATTGAGTCTATCCAGCACCCGCCAGGAAACCGGGCGGTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCGGAATGTATCGGTACAGAATCTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCAGAACAGACTGCACGGCCTGTCAGTGCAGGTTGAAATCAATCACGTCAAATTAACGGCGGTTGTGGACGGCTTTGCCAGCGa                                                <  3:436934/275‑1 (MQ=255)

                                                                                                                                                                                                                                cTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAACACGCCATCGCGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCACTCACGTCAAATTACCGTCGGTGGTGGACGGCTTTGCCAGCgca                                               >  2:317781/1‑272 (MQ=255)

                                                                                                                                                                                                                                cTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGTTTTCGAATAATAAACTGCACGGCCAGTCAGTGCAGGTTGAAACCAATTACGTAAAATTACCGGCGGTGTGGGACGGTTTTGCCAGCGa                                                >  1:457962/1‑273 (MQ=255)

                                                                                                                                                                                                                                cTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGcccgagac                                               >  2:410781/1‑268 (MQ=255)

                                                                                                                                                                                                                                   taTTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGa                                           <  2:266833/274‑1 (MQ=255)

                                                                                                                                                                                                                                    aTTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGa                                           <  1:312920/274‑1 (MQ=255)

                                                                                                                                                                                                                                          aCTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTTAAATTACCGGCGGTGGTGGACGGCt                                                          >  1:198672/1‑253 (MQ=255)

                                                                                                                                                                                                                                          aCTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGACGGCTGTGGCAGGGAAGGGAGCATccc                                    >  1:115755/1‑274 (MQ=255)

                                                                                                                                                                                                                                                       caACTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATTCAGTTCTTTTTCgaa                       >  2:4928/1‑276 (MQ=255)

                                                                                                                                                                                                                                                         aCTGGTTTCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGTGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCgaaga                     <  2:438616/276‑1 (MQ=255)

                                                                                                                                                                                                                                                           tgttttCTGAGTGTATTGAATTGGGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAACCCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGaaa                   <  2:19249/273‑1 (MQ=255)

                                                                                                                                                                                                                                                               ttCTGGGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACg                 <  1:320677/274‑1 (MQ=255)

                                                                                                                                                                                                                                                               ttCTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACTGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGttct                                                                                                                                                                                                    >  4:220046/1‑95 (MQ=255)

                                                                                                                                                                                                                                                                 cTGCGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGc                                                      >  3:270357/1‑235 (MQ=255)

                                                                                                                                                                                                                                                                 cTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGc                                                      <  1:270357/235‑1 (MQ=255)

                                                                                                                                                                                                                                                                 cTGAGTGTATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACg                 >  4:363441/1‑272 (MQ=255)

                                                                                                                                                                                                                                                                     tggtATTTAATTACGTCTTTCCCGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTTTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACGGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGAc           <  3:457962/272‑1 (MQ=255)

                                                                                                                                                                                                                                                                        tATTGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGaa      <  2:66630/276‑1 (MQ=255)

                                                                                                                                                                                                                                                                        gaTTGAAGTGGGTCTTTCCAGCCCCCGGAAGGAAACCGTGGGTTTCAAGGCGCTGCGCGGTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGGACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACgaga       <  3:115755/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                          ttGAATTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAg                                              >  2:63641/1‑234 (MQ=255)

                                                                                                                                                                                                                                                                             aaTTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGTGCGATTTCGCATAACAAACTGCATGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACTGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCTTCCAGTTCTTTTTCGAAGTAACGCTAGACCAGCAttct  >  2:158046/1‑272 (MQ=255)

                                                                                                                                                                                                                                                                             aaTTGAGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGa                                           <  4:63641/234‑1 (MQ=255)

ATTTCCGTAAGCTGGATGTCTTCAGCTTTGGCGAGAATCAAAATTGCCTGGTAGGTAGCGTTGACCTGCTGTACCGCAACTCGTGGAACGAAG‑TGCGTACGCTGCACTTCAACGGCGAGCAATCGATGATCGAAGCCCTGAAAACTATTCTCGGCAAAATGCATCAGGACGCCGCACCGCCAGATAGCGTGGAAGTCTTCTGTTATAGCCAGCATCTGCGCGGCTTAATTCGTACTCGCGTGCAG‑CAACTGGTTTCTGAGTGTATTGAATTGCGTCTTTCCAGCACCCGCCAGGAAACCGGGCGTTTCAAGGCGCTGCGCGTTTCTGGTCAAACCTGGGGGTTGTTCTTCGAACGCCTGAATGTATCGGTACAGAAACTGGAAAACGCCATCGAGTTTTATGGCGCGATTTCGCATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGC  >  NC_000913/3992873‑3993414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`