BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,679,434:1	+C	5.7%	intergenic (‑67/+30)	yphF ← / ← yphG	putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,434	1	.	C	5.7%	904.3 / 50.8	209	intergenic (‑67/+30)	yphF/yphG	putative sugar ABC transporter periplasmic binding protein/DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand): ref base . (92/105); new base C (5/7); total (97/112)
Fisher's exact test for biased strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-03

Only 100 of 212 total aligned reads displayed.

TAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAG  >  NC_000913/2679166‑2679683

tagtgtgcaTTAACGTAACTTGGACTGAGGAATCTTTTGCAGCATCCTGGACGCCCTGACGCATTCCAGCGTAGTATCCCTGAGTATCAAGGTATAGTGCGCCAATGGTCATTTCTTTTGCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCTATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGc                                                                                                                                                                                                                                                     <  4:298274/267‑1 (MQ=255)

      cTATTAACTGAACTTGTAATGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATAGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATAtt                                                                                                                                                                                                                                            <  1:72599/278‑1 (MQ=255)

                                            tCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCCCAAATATTCCAGTTACAGGGGTTTAGTGAACGCGGTTAAAAAt                                                                                                                                                                                                         >  4:348261/1‑275 (MQ=255)

                                                                                  aGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGc                                                                                                                                                               >  1:127444/1‑279 (MQ=255)

                                                                                              tatTGCTCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTCGCCATTAATAATAGATTACGGGTTGCTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTATCGTTAATAGGGTTTAGTACACCCGGTTAAACATAAAAGCCATCTCGGTTGTGATTACTTCCCCTTTCCGCCCAGctgatttaa                                                                                                                                                       >  4:428435/1‑267 (MQ=255)

                                                                                              tatTGCGCCACTGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACACAGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGAATCACGGTTGTGAATCATGCCGTTTTCGCCAAAGCCGGATTaa                                                                                                                                                       >  4:413487/1‑275 (MQ=255)

                                                                                              tatTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAACAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTaa                                                                                                                                                       >  2:519649/1‑275 (MQ=255)

                                                                                                        aTGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  3:6027/183‑1 (MQ=255)

                                                                                                         tGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTa                                                                                                                                                                                                                                           <  2:336598/180‑1 (MQ=255)

                                                                                                             cATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGAGTAGCCATTAATAATTGATTACGGGTAGTCACCATTTTTGTAGGCATAGAGTCTCCTGTAGGGTTTTTATTAACAACGGACTATTCTAATTATTTTGTGATGAGCGGCATCGCG‑TGCAGCTCAAATATCACCGTTAATAGGGTTTAGTGAATTAGGTTAAAAATCAAAGGAATCCGGATGATTATTTTGTCTGATTTCGGCCAAGCCGAATTAGTCCCCGTCAATTTAg                                                                                                                                        >  4:327892/1‑275 (MQ=255)

                                                                                                                   tttttACGCGTCCCTTGTAAATAGTGCGCTACTGATTAGCGTAGCCATTAATAATAGATTACGGGTGTGTCTCAGTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTTTAATTATTTTGTGATGAGCGGCAGCGCG‑GTCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg                                                                                                                                  <  4:65355/275‑1 (MQ=255)

                                                                                                                          gCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑Tg                                                                                                                                                                                                                                                         >  7:455158/1‑149 (MQ=255)

                                                                                                                            aGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGTGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCat                                                                                                                         <  2:428439/275‑1 (MQ=255)

                                                                                                                             gCCCTGGCAATTAGTTGGCTACCTAATAGGGTAGCCATAAATAATAGCTTACGGGTTGTTCTCATTTTTGTAGGCAAAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCT‑TTCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata                                                                                                                        <  4:519645/275‑1 (MQ=255)

                                                                                                                                cTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTc                                                                                                                                                                                                                                                   <  5:1435432/149‑1 (MQ=255)

                                                                                                                                 tGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCa                                                                                                                                                                                                                                                  <  8:549564/149‑1 (MQ=255)

                                                                                                                                   gCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑Tg                                                                                                                                                                                                                                                         <  8:14805/140‑1 (MQ=255)

                                                                                                                                        tAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTa                                                                                                                                                                                                                                           <  5:1035700/149‑1 (MQ=255)

                                                                                                                                        tAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTa                                                                                                                                                                                                                                           <  6:1039382/149‑1 (MQ=255)

                                                                                                                                         aGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTAc                                                                                                                                                                                                                                          <  8:455158/149‑1 (MQ=255)

                                                                                                                                          gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  7:70480/149‑1 (MQ=255)

                                                                                                                                          gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  8:313482/149‑1 (MQ=255)

                                                                                                                                          gTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACa                                                                                                                                                                                                                                         <  6:1550905/149‑1 (MQ=255)

                                                                                                                                            gcgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGt                                                                                                                                                                                                                                       <  6:1451400/149‑1 (MQ=255)

                                                                                                                                             cgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCAt                                                                                                                                                                                                >  2:495727/1‑187 (MQ=255)

                                                                                                                                             cgcTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcccc                                                                                                         >  2:447731/1‑274 (MQ=255)

                                                                                                                                               cTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTaa                                                                                                                                                                                                                                    <  7:257712/149‑1 (MQ=255)

                                                                                                                                                tACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAAt                                                                                                                                                                                                                                   >  7:1625664/1‑149 (MQ=255)

                                                                                                                                                 aCCTAATAGCGTAGCCATTAATAATAGGTTAGGGATTGGGCTAATTTTTGTAGGCATGGAGCCTCCTGTAGGGTGTTTATTAACAACGGCTTATTGTAATTATTTTGTGATGGGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATa                                                                                                                                                                                                                                  >  6:342109/1‑149 (MQ=255)

                                                                                                                                                    tAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCTTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAggg                                                                                                                                                                                                                               >  5:1401121/1‑149 (MQ=255)

                                                                                                                                                      aTAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGtt                                                                                                                                                                                                                             <  7:545906/149‑1 (MQ=255)

                                                                                                                                                      aTAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGtt                                                                                                                                                                                                                             <  5:1516236/149‑1 (MQ=255)

                                                                                                                                                        aGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTa                                                                                                                                                                                                                           <  6:1700297/149‑1 (MQ=255)

                                                                                                                                                        aGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTa                                                                                                                                                                                                                           <  5:1489794/149‑1 (MQ=255)

                                                                                                                                                            tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGcc                                                                                                                                                                                                                                                       >  4:181006/1‑117 (MQ=255)

                                                                                                                                                            tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAAt                                                                                                                                                                                                                                   >  1:55750/1‑137 (MQ=255)

                                                                                                                                                            tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTc                                                                                                                                          >  2:331790/1‑226 (MQ=255)

                                                                                                                                                            tAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTGCGGCCAAGCCGGATTAATCTCCGTCAATTCAGCGCGAACAGACTCAGTTTGCCACACCTCCCGGAATCCCCAGGAccc                                                                                          >  4:502816/1‑272 (MQ=255)

                                                                                                                                                               ccATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcg                                                                                                                                                                                                                    >  7:1476089/1‑149 (MQ=255)

                                                                                                                                                                cATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTc                                                                                                                                                                                                                                                   <  2:181009/117‑1 (MQ=255)

                                                                                                                                                                 aTTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTc                                                                                                                                                                                                                                                   <  5:1702438/116‑1 (MQ=255)

                                                                                                                                                                        ataGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                                                                                                                                                                           <  7:1247167/149‑1 (MQ=255)

                                                                                                                                                                         taGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaaa                                                                                                                                                                                                          <  6:77687/149‑1 (MQ=255)

                                                                                                                                                                         taGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaaa                                                                                                                                                                                                          <  7:1449915/149‑1 (MQ=255)

                                                                                                                                                                           gATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCCGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGCTAATAGGGTTTAGTGAACGCGGTTAAAAATa                                                                                                                                                                                                        >  7:1016426/1‑149 (MQ=255)

                                                                                                                                                                               aCGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑Tg                                                                                                                                                                                                                                                         >  3:143526/1‑96 (MQ=255)

                                                                                                                                                                                 gggTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGc                                                                                                                                                                                                  <  6:915818/149‑1 (MQ=255)

                                                                                                                                                                                       ttCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAatat                                                                                                                                                                                                                                             >  5:1715312/1‑100 (MQ=255)

                                                                                                                                                                                        tctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACgg                                                                                                                                                                                           >  8:625899/1‑149 (MQ=255)

                                                                                                                                                                                         ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggt                                                                                                                                                                                          >  7:860638/1‑149 (MQ=255)

                                                                                                                                                                                         ctcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCA‑CGCGCTGCCGCTCa                                                                                                                                                                                                                                                  >  5:1560980/1‑93 (MQ=255)

                                                                                                                                                                                          tcgtttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                                                                                                                         >  8:1546831/4‑149 (MQ=255)

                                                                                                                                                                                          tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTTATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                                                                                                                         >  6:1707183/1‑149 (MQ=255)

                                                                                                                                                                                          tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                                                                                                                         <  8:568061/149‑1 (MQ=255)

                                                                                                                                                                                          tcATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtg                                                                                                                                                                                         >  5:1670063/1‑149 (MQ=255)

                                                                                                                                                                                            aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGCTGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                                                                                                                                       >  6:296581/1‑149 (MQ=255)

                                                                                                                                                                                            aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                                                                                                                                       >  6:371671/1‑149 (MQ=255)

                                                                                                                                                                                            aTTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                                                                                                                                       <  6:1683069/149‑1 (MQ=255)

                                                                                                                                                                                             tttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGgtggtg                                                                                                                                                                                      >  5:1289124/1‑149 (MQ=255)

                                                                                                                                                                                              ttttGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGa                                                                                                                                                                                     <  6:534928/149‑1 (MQ=255)

                                                                                                                                                                                                   tAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaataat                                                                                                                                                                                <  7:418918/149‑1 (MQ=255)

                                                                                                                                                                                                      gCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAgg                                                                                                                                                                                                   <  8:673369/127‑1 (MQ=255)

                                                                                                                                                                                                       cATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCg                                                                                                                                                                            <  6:1664788/149‑1 (MQ=255)

                                                                                                                                                                                                          agagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCa                                                                                                                                                                                              <  2:278019/128‑1 (MQ=255)

                                                                                                                                                                                                           gagCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCttt                                                                                                                                                                        >  6:1402820/1‑149 (MQ=255)

                                                                                                                                                                                                            agCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTc                                                                                                                                                                       <  8:1016426/149‑1 (MQ=255)

                                                                                                                                                                                                             gCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCg                                                                                                                                                                      <  6:585838/149‑1 (MQ=255)

                                                                                                                                                                                                              cctcctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCgg                                                                                                                                                                     <  8:12956/149‑1 (MQ=255)

                                                                                                                                                                                                                 cctGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg                                                                                                                                  <  1:239881/181‑1 (MQ=255)

                                                                                                                                                                                                                     tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAgg                                                                                                                                                                                                   <  4:105156/112‑1 (MQ=255)

                                                                                                                                                                                                                     tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACggtggt                                                                                                                                                                                       >  8:138347/1‑124 (MQ=255)

                                                                                                                                                                                                                     tAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGcc                                                                                                                                                              >  5:437076/1‑149 (MQ=255)

                                                                                                                                                                                                                        ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCCGCCAAGCCGGATTAATCGCCGTCAATTCAgcg                                                                                                                                      >  2:594437/1‑170 (MQ=255)

                                                                                                                                                                                                                         gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                                                                                                                          <  6:1289124/149‑1 (MQ=255)

                                                                                                                                                                                                                         gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAt                                                                                                                                                          <  6:1472848/149‑1 (MQ=255)

                                                                                                                                                                                                                          tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAACAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCACCCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAACTCGCCATACAGCGacaa                           >  2:668942/1‑275 (MQ=255)

                                                                                                                                                                                                                          tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCa                                                                                                                                                                                                 >  5:1243579/1‑109 (MQ=255)

                                                                                                                                                                                                                          tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGaataat                                                                                                                                                                                <  5:469850/126‑1 (MQ=255)

                                                                                                                                                                                                                          tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGTCGGAtt                                                                                                                                                         >  6:954039/1‑149 (MQ=255)

                                                                                                                                                                                                                          tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGAtt                                                                                                                                                         >  7:778302/1‑149 (MQ=255)

                                                                                                                                                                                                                            tttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGt                                                                                                                                                >  2:695716/1‑156 (MQ=255)

                                                                                                                                                                                                                            tttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCCGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg                                                                                                                                  <  4:594433/170‑1 (MQ=255)

                                                                                                                                                                                                                                   acaacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGt                                                                                                                                                >  5:902747/1‑149 (MQ=255)

                                                                                                                                                                                                                                    caacGGCTGATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGGTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGAGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAgcg                                                                                                                                      >  2:424503/1‑158 (MQ=255)

                                                                                                                                                                                                                                       cGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAg                                                                                                                                                                >  3:182602/1‑129 (MQ=255)

                                                                                                                                                                                                                                          cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGTCGGATTAATCGCCGTCAATTCa                                                                                                                                         <  5:954039/149‑1 (MQ=255)

                                                                                                                                                                                                                                          cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCa                                                                                                                                         <  8:1625664/149‑1 (MQ=255)

                                                                                                                                                                                                                                            tATTCTAATTATTTTGGGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTc                                                                                                    <  3:273137/184‑1 (MQ=255)

                                                                                                                                                                                                                                               tCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAg                                                                                                                                        >  1:180676/1‑145 (MQ=255)

                                                                                                                                                                                                                                                   attattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAg  >  2:146528/1‑275 (MQ=255)

                                                                                                                                                                                                                                                    ttattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAg                                                                                                                               >  8:267032/1‑149 (MQ=255)

                                                                                                                                                                                                                                                     tattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                                                                                                                              <  6:195137/149‑1 (MQ=255)

                                                                                                                                                                                                                                                     tattTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                                                                                                                              <  5:222671/149‑1 (MQ=255)

                                                                                                                                                                                                                                                        tttGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAg                                                                                                                                  <  1:9458/142‑1 (MQ=255)

                                                                                                                                                                                                                                                           gtgATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAGTTCAGCGCGAGCAGACTCata                                                                                                                        >  5:194228/1‑149 (MQ=255)

                                                                                                                                                                                                                                                            tgatgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTaaaa                                                                                                                                                                                                           <  7:630399/65‑1 (MQ=255)

                                                                                                                                                                                                                                                             gatgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt                                                                                                                      >  6:1195325/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               tgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTa                                                                                                                                                                                                                           <  7:1491354/46‑1 (MQ=255)

                                                                                                                                                                                                                                                               tgaGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGGTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc                                                                                                                    >  5:184883/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                       aGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTc                                                                                                            <  7:1225174/149‑1 (MQ=255)

TAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCG‑TGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGCGAGACGGCAAAG  >  NC_000913/2679166‑2679683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`