| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,180,505 | G→T | 9.1% | E29* (GAG→TAG) ‡ | ygiC → | ATP‑Grasp family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,180,505 | 0 | G | T | 9.1% | 887.7 / 61.8 | 266 | E29* (GAG→TAG) ‡ | ygiC | ATP‑Grasp family ATPase |
| Reads supporting (aligned to +/- strand): ref base G (120/121); new base T (11/13); total (131/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-02 | |||||||||||
Only 100 of 271 total aligned reads displayed.
CGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAA > NC_000913/3180229‑3180767 | cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAGAGAGTCAGTATTACCGAGCGCCCGGCCTGGCGTGAGAAAGCCCACGCATACGGTTTCAATTTTCACACCATGTACGGCGAg > 1:291867/1‑279 (MQ=255) acgGGTAAAGAGGATGGCCCGCCCCACCACGGCCGGACCATTACCGAACCGAAGACGCGCATGGAACCAAATCCGGTGAACACCACTACCGTTACACGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAg < 4:50018/275‑1 (MQ=255) cagcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGAGTAGTGCAACCGGTACCTCTTCTCGTTCAATGTGTGGCTGATACCGATGGACGGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGACGTAC‑TGGTGTGAAGCTGCTta > 1:167038/1‑279 (MQ=255) cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTTGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTCCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAACGGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATAAGGTTTCAAATTTCACACCATGTTCCGCGAACCGGAC‑TGGGGGAAAGATGCt > 4:266250/1‑274 (MQ=255) ccgcccgGACGCACCATGACAGTACCGAAGACGGAAAGGGCACCAAAACCGGGGACCACCACCACCGTGACCCGTGGAGGTGTTGGTGCATCTGTGTCCAAACAAATCACTAGGCAGCGTAGGGCGACCGGTACCTCTTCCCGTTCAATGGGTGGCTGAAACCGATGGAGAGAGTCAGCATTACCGAGCGCCCGGACTGGCGTTAGAAAGCCCACGAATACGGTTTCACTTTCCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtac < 4:639488/273‑1 (MQ=255) aCCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATAAGGTTTCAATTTTCAAACCATGTAAGGCGAGCCGTAC‑GGGTGTGAAGAGGCTTACTAAAAGTTGAcc > 2:329937/1‑275 (MQ=255) tttGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCa > 2:438048/1‑253 (MQ=255) gTGAATCTGTTTCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtg < 4:438044/253‑1 (MQ=255) tCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGGGGTGGAAAAAGTg > 2:535403/1‑272 (MQ=255) aacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg > 5:282266/1‑149 (MQ=255) acaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAAGACGGTTTCAATTTTCAGACCATGTACGGCGa > 8:1193959/1‑149 (MQ=255) cACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTa < 6:1520086/149‑1 (MQ=255) tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCTCGCCGTAcatgg < 7:897185/149‑5 (MQ=255) tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatggt < 5:1082353/149‑6 (MQ=255) gCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtg > 8:1672890/1‑149 (MQ=255) gCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtg < 7:1672890/149‑1 (MQ=255) gCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTACATGgtg < 5:1714353/149‑1 (MQ=255) cAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtgt < 7:1563366/149‑1 (MQ=255) cAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtgt < 7:1588188/149‑1 (MQ=255) aGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGtt > 2:227693/1‑168 (MQ=255) aGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGAt < 5:1516626/149‑1 (MQ=255) tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGcc < 3:278951/171‑1 (MQ=255) aaCCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTta < 7:1321616/149‑1 (MQ=255) ccGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtact < 6:1111088/149‑1 (MQ=255) tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatgg < 5:165367/125‑5 (MQ=255) cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtg < 2:524565/202‑1 (MQ=255) ttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGa < 5:71669/128‑1 (MQ=255) gTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCg > 5:1546859/1‑149 (MQ=255) ttCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGc > 8:835532/1‑149 (MQ=255) tCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCGCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCGTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACCCCTGGAGtttt > 2:123321/1‑275 (MQ=255) aaTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCa > 8:64402/1‑149 (MQ=255) aTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAc > 7:505962/1‑139 (MQ=255) tGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑Tgg > 7:305389/1‑112 (MQ=255) tGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAg > 4:464409/1‑119 (MQ=255) tGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc < 2:464413/119‑1 (MQ=255) ggCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCTCCCAGGTTGaa < 7:1111048/149‑1 (MQ=255) tGATACCGATGGAAATAGTCAGTATTACCGCGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATCTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACGAGTTGACCCTCGCCCAGGTTGAAAAg > 8:1044824/1‑149 (MQ=255) tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAg > 7:1007406/1‑149 (MQ=255) tACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt < 5:943219/102‑1 (MQ=255) aCCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgcc > 2:371698/1‑163 (MQ=255) tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa < 7:571346/149‑1 (MQ=255) tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa < 8:585732/149‑1 (MQ=255) ggAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGGGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGaagaag > 8:1596372/1‑149 (MQ=255) gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTg < 7:225360/133‑1 (MQ=255) gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTg > 8:225360/1‑133 (MQ=255) gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTACACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGt < 5:495701/149‑1 (MQ=255) aaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAAGACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCAc < 2:567526/274‑1 (MQ=255) gagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAcc > 8:921906/1‑149 (MQ=255) gTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGAt < 5:214958/96‑1 (MQ=255) gTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGAt > 6:214958/1‑96 (MQ=255) gTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgc > 6:857574/1‑149 (MQ=255) tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCt > 6:276138/1‑98 (MQ=255) tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCt < 5:276138/98‑1 (MQ=255) aGTATTACCGAGAGCCCGGACTGGCGTGAGAAAGCCCACGAATACGATTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGa > 6:1459987/1‑149 (MQ=255) tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATACCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTa > 1:204661/1‑279 (MQ=255) aCCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCCCGCCAAGGTTGACAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACCCTCGGAGTTTTTTTCGCCAGTCATGGCTGCCGCCCCTGCCATTGCttca > 2:94803/1‑273 (MQ=255) ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCt > 8:1451952/1‑89 (MQ=255) ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCt < 7:1451952/89‑1 (MQ=255) ccGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc < 7:447006/149‑1 (MQ=255) aGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGGGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTc > 1:172329/1‑279 (MQ=255) gcgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa > 6:185036/1‑149 (MQ=255) cgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAt < 6:1546859/149‑1 (MQ=255) cgcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATNACCAAATTCCGCANTcn > 4:724041/1‑211 (MQ=255) cccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt > 8:189378/1‑149 (MQ=255) ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaaaa > 8:1358230/1‑113 (MQ=255) ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa < 7:81288/123‑1 (MQ=255) ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgtg > 7:1365726/1‑149 (MQ=255) cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc < 6:614358/149‑1 (MQ=255) cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc < 7:182657/149‑1 (MQ=255) cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCaaaa < 2:724046/212‑1 (MQ=255) gACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCt < 7:361337/149‑1 (MQ=255) gcgtgctgggagaaGCCCCAGTATACGGGTTCAATTTTCACACCAGTAAGGGCGACCCGTAC‑TGGTGTGAAGATGCTTACTACAATTGGTCCCGCGCCCAGGTTACAAAGCGGGAAGAAGTCACCGCCAAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAACAAATTCCGCATGCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGGACCAGCAATCGCTTTATTCGCGTCTTGAt < 4:329933/263‑1 (MQ=255) acgggCGTGAGAACGCCCCAGAATACGGGTTAAATTTTCACACCATGTAAGGCGGGCCGTAC‑TGGGGTGAAGATGCTTACTACAAGTTGACCCTAGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGAt < 4:543929/272‑1 (MQ=255) aCTGGCGTGGGAAAGCCCACGAATACGGGTTCAATGTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGGTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGAt < 4:139714/275‑1 (MQ=255) gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTGGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggt < 5:1590579/149‑1 (MQ=255) aaaGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGa > 8:497561/1‑149 (MQ=255) ccACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAg > 6:678522/1‑149 (MQ=255) cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGt > 7:694193/1‑149 (MQ=255) cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGt < 5:140318/149‑1 (MQ=255) cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGt < 6:160296/149‑1 (MQ=255) aaTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATc < 6:780219/149‑1 (MQ=255) aCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGcc > 6:1362373/1‑149 (MQ=255) tttCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAACGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCg > 6:1042172/1‑149 (MQ=255) ttCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATc > 7:1251415/1‑141 (MQ=255) tCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGGAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGAt < 6:473228/149‑1 (MQ=255) tCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGAt < 7:1491529/149‑1 (MQ=255) ttCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtg > 8:1203239/1‑149 (MQ=39) cacaCCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgat < 8:381697/149‑1 (MQ=255) cacaCCATATCCGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCTCACAGGTTGAAAGGATGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGATAGTGGCCGAACAAGTGCTCTCCAGCGCTGAGCTGATGACCAAATCCAACATTCCAAAACACCCCTGGAGTTTTGAGCGCCACTACTGGCTGCGGCACCACCCCTCCCTTTATTCGCGTCTTGATCCGGCGTGGGAGGGCCGTCGGTAACCAAAACTTATGGa > 4:96674/1‑275 (MQ=255) acacCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatg > 8:473677/1‑149 (MQ=255) acacCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatg > 8:1682771/1‑149 (MQ=255) acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgcc > 8:509310/1‑87 (MQ=255) acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 8:1563056/1‑149 (MQ=255) accagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatga < 7:979849/144‑1 (MQ=255) accagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc > 6:356821/5‑149 (MQ=255) ccagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGAt > 6:747591/4‑137 (MQ=255) cATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAACAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCa > 7:402131/1‑149 (MQ=255) cATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATg > 5:84338/1‑137 (MQ=255) cagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtga < 7:601489/141‑1 (MQ=255) aCGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTc > 5:589585/1‑149 (MQ=255) | CGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAACACACCTGGAGTTTTGTGCGCCAGTCATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAA > NC_000913/3180229‑3180767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |