BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	257,908	Δ776 bp		[crl]	[crl]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	257908–258675	258683	9–776	146 [0]	[0] 146	[crl]	[crl]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 257907	0 (0.000)	138 (1.040)	94/502	0.0	100%	intergenic (+8/‑769)	crl/crl	pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
*	?	NC_000913	258684 =	0 (0.000)	138 (1.040)	94/502	0.0	100%	pseudogene (9/331 nt)	crl	pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 146 total aligned reads displayed.

TGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTG  >  NC_000913/258414‑258958

aagaggactcacgcttaatcacctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttgggaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggaccgtatattcgtgaaggtaAgta                                                                                                                                                                                                                                                                                   >  2:567469‑M2/271‑273 (MQ=255)

  ggagtacccccattaatcacctcatctatttctgaaggtaaattattcgatatcccatttaacccggtgtaacgtaattttgacaaaggacttcaggaaatcaccgactggatcgaaaaacgcttgtgtgaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCaa                                                                                                                                                                                                                                                                                <  4:70552‑M2/7‑1 (MQ=255)

      actcacgcttaatcacctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggatacccgaagacaagattgatcaaaaaatttaccgcactaggcccgcaaattcgttaaggaaagtgccgttct                                                                                                                                                                                                                                                                            >  3:587416‑M2/265‑269 (MQ=255)

          acgcttaatcacctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaattttctaaattttgccaaattggtaaaacagttgcatcacaaaaggaaatagcaatgacgttaccgagtggacacccaaagagtagattactaaaaatattgaccgcactatgccagtagctacgggaaggtaAGTTAAAaaataata                                                                                                                                                                                                                                                                        >  4:321406‑M2/261‑274 (MQ=255)

          acgcttaatcacctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGTTAATc                                                                                                                                                                                                                                                                        >  2:437178‑M2/261‑275 (MQ=255)

            gcttattcatctcagcctctggtgaccgtaaattattagagataccatttaaccaggtgtagcggaatttttacaaaggtcttcatgaaatcaccgactgtatctaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                                                                                                                                                      <  4:383887‑M2/17‑1 (MQ=255)

                      ctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                                                                                                                                                            >  1:246226‑M2/249‑275 (MQ=255)

                      ctcatcatctgctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgagaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                                                                                                                                                            >  1:110036‑M2/249‑275 (MQ=255)

                       tcatcatatgctgacggtaaattattagagatcacatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgataaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                                                                                                                                                           <  4:116769‑M2/28‑1 (MQ=255)

                         tgcatctactgacggtaaattatgagagatcccatttagccgggttaatgcgaattttgacaaagtgattcaggaaatcaccagctggatcgaaaaacgctttggttaaaaatttgctaaattttgccaatgtggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                                                                                                                                                         <  1:362631‑M2/30‑1 (MQ=255)

                         atcatctgctgacggtaaattattagagatcccatttaaccaggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                                                                                                                                                         <  2:213128‑M2/30‑1 (MQ=255)

                           catctgcgaaacgtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                                       <  1:182577‑M2/32‑1 (MQ=255)

                            atctactgaaggtaaattattagagatccaatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgtgaaaaatttgctaaattttgccaatgtggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                                       <  3:18526‑M2/32‑1 (MQ=255)

                                gctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagagtgatcaaaaaatttaccgcactagacgcgtatattcgagaaggtaAGTGCAAAGATAAGCGATACTTTTTCGATTTTCTGGc                                                                                                                                                                                                                                                  >  2:659333‑M2/239‑275 (MQ=255)

                                gctgacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggaaacccgaagagaagaatgatcaaaaaatttaacgcactaggcccgtatattcggaaaggtaAGTGAAAAAATAAACGATTCTTTTTCGATTGTCTGGc                                                                                                                                                                                                                                                  >  4:225628‑M2/239‑275 (MQ=255)

                                   gacggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                                                                                                                                                               >  1:104922‑M2/236‑275 (MQ=255)

                                    acggtaaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaattacgttaccgagtggaaacacaaaaaacagattgataaaaaaatttacagaactaggccagaataagcgtgaatgtaAGTGCAAAGATAATCGATTCTTTTTCGTTTGTCTGGCTGTa                                                                                                                                                                                                                                              >  4:405460‑M2/235‑275 (MQ=255)

                                        taaattattagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgacgagcagattgatcaaaaaatttaccgcactaggcccgaatattcgtaaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTTTCTTTCTGTATTCg                                                                                                                                                                                                                                          >  4:422405‑M2/231‑275 (MQ=255)

                                                tagagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTcaactgg                                                                                                                                                                                                                                  >  1:802103‑M2/223‑272 (MQ=255)

                                                  gagatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                                                                                                                                                                                                                >  1:21879‑M2/221‑275 (MQ=255)

                                                    gatcccatttaacccggtgtatcggaattttgacaatggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttacagcactaggcacgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGCTTGCGTCAACGTGa                                                                                                                                                                                                                                 >  1:126379‑M2/219‑272 (MQ=255)

                                                    gatcccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgcatattcgtgaaggtaAGTGCAAAGATAATCGATTCTCTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                                                                                                                                                                                                               >  1:454207‑M2/219‑274 (MQ=255)

                                                      tccatttaaacccgtggaacggaaattttgacaacgtcttacagaaaacaaccaatggaatgaaaaaagactttgttaaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggtgatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                                                                                                                                                             <  2:150859‑M2/58‑1 (MQ=255)

                                                       cccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagcttgatcaaaaaatttcccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGCTTGTCTGGCTGTATGCGTCAACGTGCAACcgc                                                                                                                                                                                                                           >  2:316080‑M2/216‑274 (MQ=255)

                                                         catttaacccggtgtatcggaattttgaaaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCa                                                                                                                                                                                                                         <  3:708852‑M2/62‑1 (MQ=255)

                                                             taacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagatgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgataaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCAGGCTGTATGCGTCAACGTGAAAACGGCACCgg                                                                                                                                                                                                                     >  2:597018‑M2/210‑275 (MQ=255)

                                                                gcctgtgtatcggaatattgacaaagttcttcagggaatcaccgactgagtcggaaaactgttgtgttaaaaatttgctaaattgttccaatttgttaaaacagttgcatcaccacaggagagagcaatgacgttaccgagttgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                                                                                                                                                                  <  3:454000‑M2/69‑1 (MQ=255)

                                                                    gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                                                                                                                                                 >  1:363337‑M2/203‑272 (MQ=255)

                                                                    gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGt                                                                                                                                                                                                                 >  2:101046‑M2/203‑272 (MQ=255)

                                                                     tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                                                                                                                                                           >  1:444766‑M2/202‑229 (MQ=255)

                                                                         tcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                                                                       <  3:444766‑M2/32‑1 (MQ=255)

                                                                          cggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGTCACCGGAAGTGCGTGAAtt                                                                                                                                                                                                        >  2:518471‑M2/197‑275 (MQ=255)

                                                                               ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactcggcccgtatattcgtgaagataAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTATggg                                                                                                                                                                                                   >  2:551588‑M2/192‑275 (MQ=255)

                                                                               ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtctattcgtgaaggtaAGTGCAAAGATAATCTATTCTTTTTCGATTGTCTGGCTGTATGCGTCACTGTGAAACCGGCACAGGAAGTGCGTGAATTCTggg                                                                                                                                                                                                   >  2:531321‑M2/192‑275 (MQ=255)

                                                                                tttgacaaagggcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgacaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                                                                                                                                                  <  2:33081‑M2/85‑1 (MQ=255)

                                                                                   gaaaaaaatctgcaggaaataaccgacaggatcgaaaaacacttgtgttaaaaagtggagaaatgttgacaattgggtaaaacaagtgaataacaacaggagatagcaatgacgttaccgagtggacagccgaagagcagagtgattaaaaaatttaccgcactaggcccggatattcgggaaggtaAGTGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg                                                                                                                                                                                               <  4:437178‑M2/88‑1 (MQ=255)

                                                                                    tacaaggtcttaagggaatcaccgactggatcgaaaaacgcttgttttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtg                                                                                                                                                                                               <  4:411659‑M2/88‑1 (MQ=255)

                                                                                         ggtcttgaggaaatcaacgaagggaacgaaaaacgcttgtgttaaaaatttgctaaattatgccaattgggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                                                                                                                                                         <  2:8533‑M2/94‑1 (MQ=255)

                                                                                         ggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgctaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGGGTCAACGAGAAACCGGCACTGGatgtgcgtgatttcctgtctcttatcc                                                                                                                                                                                          >  3:695180‑M2/182‑261 (MQ=255)

                                                                                             tgcaggaaatcaccgactggatcgaaaaacgcttgttttaaaaatttgctaaattgtgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                                                                                                                                                                  <  1:695180‑M2/69‑1 (MQ=255)

                                                                                                              aacaaagaaaaacgcttaggtaaaaaatttgctagatttagccaatttggtaaaacagttgcaaaacaacaggagatagaaattacgttaccgagaggacacccgaatagcagattaattaaaaaatttacctcactaggccagtatattcttgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTTGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATc                                                                                                                                                                   <  3:631902‑M2/115‑1 (MQ=255)

                                                                                                                  tcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAAGCTGGAAGCGCAGGAATCCCg                                                                                                                                                                >  1:477478‑M2/157‑275 (MQ=255)

                                                                                                                  tcgaaaaacgcttgtgttaaaaattggctaaaatttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGt                                                                                                                                                               <  2:422405‑M2/119‑1 (MQ=255)

                                                                                                                  gagaaacacgattgtgttaaaaatttgataaattgtgccaatatggtaaaacagttgcagcacaacaggagatagcaatgacgttaccgatgggacaaccgaagagcagattgatcaaaaaatttacctcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACTTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGt                                                                                                                                                               <  2:466504‑M2/119‑1 (MQ=255)

                                                                                                                    gaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactcggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGCTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTTAAACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGAGGG‑AGCTGGAAGCGCAGGAATACCGtt                                                                                                                                                              >  2:570959‑M2/155‑274 (MQ=255)

                                                                                                                     aaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                                                                                                                                                           <  1:52523‑M2/60‑1 (MQ=255)

                                                                                                                       aaacgcttgtgttaaaaatttgctaaattttgccattttggtaaaaccgttgaatcaaaacaggagatagcaatgacgttaccgagtggacacgcgaagggcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTAc                                                                                                                                                          <  3:572438‑M2/124‑1 (MQ=255)

                                                                                                                              tgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactagtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTTTTTTTCGATTGTCTGGCTGTATGCGTCAACTTGAAACCGGCACCGGAAGTTCGTTAATTCTGGGGCTGGTGGATGG‑AGCCGGAAGCGCAGGAATCCGGATTTAcctatgt                                                                                                                                                    >  1:631902‑M2/145‑271 (MQ=255)

                                                                                                                                tgttaaaaatttgctaaatttttccaatttggtaaaacagttgaatcaaaacaggagatagcaatgacgttaccgagtggacacccgaagagcaaattgatcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                                                                                 <  4:388182‑M2/133‑1 (MQ=255)

                                                                                                                                tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgaagttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTAGATTGTCTGGCTGTATGAGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGCGGGATGG‑AGATGGAAGCGCAGGAATCCCGTTTACCCTAAAGCTa                                                                                                                                                 >  2:282714‑M2/143‑275 (MQ=255)

                                                                                                                                tgttaaaaatttgataaagtttgccaatttggtaaaacagttgcataacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgtactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                                                                                 <  3:246226‑M2/133‑1 (MQ=255)

                                                                                                                                     aaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCAGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCCTGAATTCTGGGGCTGGTTGATGG‑ATCTGGAAGCGCAGCAATCCCGTTTTACCTACAGTTACCAGt                                                                                                                                            >  2:385463‑M2/138‑275 (MQ=255)

                                                                                                                                        attgacaaaattttgacaatttggaaaaacagctgcatcaaaagaagagatagaaattacgttaccgagtggacaccctaagagcagattgaacaaaaaatttaccgcactaggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg                                                                                                                                         <  4:518471‑M2/141‑1 (MQ=255)

                                                                                                                                        aattgctaaaacttgccaatttggtaaaacatgtgcagcacaacaggagatagcaaagacgttaccgagtggacacccgaagagcatattgataaaaaaatttaccgcactaggcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTg                                                                                                                                         <  4:570959‑M2/141‑1 (MQ=255)

                                                                                                                                          ttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt                                                                                                                                       >  2:94002‑M2/133‑275 (MQ=255)

                                                                                                                                             gtaaatttttccaatttggtaaacaagtggcctcaacacaggagatagcaatgacgttaccaagtgaacacccgaagagcaaattgataaaaaaatttaccgcactaagcccgtatattcttgaaggtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg                                                                                                                                    <  1:769231‑M2/146‑1 (MQ=255)

                                                                                                                                                                        ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtaaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAACGGCACCGGCAGTGCGTGATTTTAGGGGCGGGTGGATGG‑AGCCGGAAGCGCAGTAAACCCGTTTTACCTAAAGTTACCTGTTTGGTCTGTTTGGTCAGGCCGGCGCTTGGAaga‑g                                                                                                         >  3:355010‑M2/103‑274 (MQ=255)

                                                                                                                                                                         tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑gtg                                                                                                       >  1:548094‑M2/102‑275 (MQ=255)

                                                                                                                                                                         tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGAGACTGGAAGA‑gtg                                                                                                       >  1:160261‑M2/102‑275 (MQ=255)

                                                                                                                                                                             gcaaaaaaggggatagcaatgacgttaccgagtggacacccgaaaagcagattgatcaaaaaatttaccgaactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTc                                                                                                    <  2:35811‑M2/177‑1 (MQ=255)

                                                                                                                                                                                       agagaaaaagaatgttacagtgtggacaacggaagggcaaattgaacaaaaaatttaccgaactaggtccgtatattcttgaaggttGATGCGAAGATAATCGATTTTTTTTTGATTGTGTGGCTGTATTCGTCAACGTGAAACCGGCACCTGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACATACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGac                                                                                         <  3:802103‑M2/188‑1 (MQ=255)

                                                                                                                                                                                        gatagcaatgacgttaccgagtggacacccgaagcgcagattgatccaaaaatttaccgcactaggcccgcatattcgcgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTTTCTGTCTGTATGCGTCAACGTGAAACCGACACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAAGCCCGTTTTACCGACAGTTACCTGTTTGGTCTGTTCGTTGATGCAGGCGTCGTGAAGAGGTGTTcgggtaaggtga                                                                                         >  4:604444‑M2/87‑270 (MQ=255)

                                                                                                                                                                                           agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAANGATAATCGATTATTTTTCGCTTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGATGG‑AGCTGGAAGCGCAGGAATCACGTGTTATCTACAGTTACCAGTTTGGTTTGTTGGATAAAGCAGGCTACTGGAGGA‑GGGttcaggtaaaaaagcatg                                                                                     >  4:365320‑M2/84‑268 (MQ=255)

                                                                                                                                                                                           agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACTTACAGTTACAAGTTTGGTCTGTTCGATAAAGAAGGCGACTGGAAGA‑GTGTTCCGGTAAAATACACt                                                                                      >  1:396252‑M2/84‑274 (MQ=255)

                                                                                                                                                                                           agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTCCAGTTACCAGTTGGGTCGGTCCGATAAAGCAGGCGACTGGACGA‑TTGTTACGGTAAAAGACCCt                                                                                      >  3:60095‑M2/84‑274 (MQ=255)

                                                                                                                                                                                              aatgaagtgaccgggtggaaaaccgaaaagcagattgataaaaaaaattacatcacttggcccgtatattcgtggaggtaAGTGCAAAGATAATCGATTCTGTGTCGATTGTTTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTATACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAg                                                                                  <  3:347876‑M2/195‑1 (MQ=255)

                                                                                                                                                                                               attaccttaccgagttgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGaca                                                                                        <  4:110105‑M2/189‑1 (MQ=255)

                                                                                                                                                                                                   cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGAGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGAGACTGGAAGA‑GTGTTCCGGTAAAAGACCATGAAGTGgtg                                                                             >  1:72264‑M2/76‑274 (MQ=255)

                                                                                                                                                                                                      taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGa                                                                           >  2:297513‑M2/73‑274 (MQ=255)

                                                                                                                                                                                                       accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTATAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑gt                                                                                                        >  1:735935‑M2/72‑244 (MQ=255)

                                                                                                                                                                                                       accgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCACGTTTTACCTACAGTTACCCGGTTGGTCTGTTCGATAAAGCAGGCGACTGGCAGA‑GTGTTCCGGGAAAATGCATCGAAgtggtggca                                                                          >  4:579534‑M2/72‑270 (MQ=255)

                                                                                                                                                                                                           agtggacacccggagagcagattgatcaaaaaatttgccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACt                                                                     <  1:589315‑M2/208‑1 (MQ=255)

                                                                                                                                                                                                           agtagacaccctaagggaagatttatgaaaaaatttaccgcaattggcccgtattttcgtgaaggtaAGTTCAAAGAGAATCGATTCTTTTTCGATTGTCTAGCTTTATGCGTCAACGTGAAACCGGCAGCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACt                                                                     <  3:110036‑M2/208‑1 (MQ=255)

                                                                                                                                                                                                             tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGTGTGAATTCTGGGGCTGGTGGCTGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCGGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTCCCGGTCAAAGACACTGAAGTGGTTGGACGACggg                                                                   >  3:743313‑M2/66‑275 (MQ=255)

                                                                                                                                                                                                             tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGTAAGA‑GTGTTCCGGTAAAAGACACGGAAGTGGTTGAACGACggg                                                                   >  1:765265‑M2/66‑275 (MQ=255)

                                                                                                                                                                                                                acaaccaaagaatagattgatgaaaaaatttaccgaacttgtcgcatatattcgtgaaggtaAGTGCAAAGAGAATCGATTCTTGTTCGATTGTCTGGCTGTAGGCGTCAACGTGAAACCGGCGCCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAg                                                                 <  3:548094‑M2/212‑1 (MQ=255)

                                                                                                                                                                                                                 cacccgaagagcagattgatcaaaaaatttaccgtacttggaccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATGGTCGGGCTGTAGGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGca                                                               <  1:330333‑M2/214‑1 (MQ=255)

                                                                                                                                                                                                                 cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGca                                                               <  1:60095‑M2/214‑1 (MQ=255)

                                                                                                                                                                                                                  acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCCGTTTGGTCTGTTCGATAAAGCCGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGcac                                                              >  2:383009‑M2/61‑275 (MQ=255)

                                                                                                                                                                                                                   ccgtaagaggagattaatcaacaaagttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGcaca                                                             <  1:321685‑M2/216‑1 (MQ=255)

                                                                                                                                                                                                                    ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatagtcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCacac                                                            <  2:579534‑M2/217‑1 (MQ=255)

                                                                                                                                                                                                                      gaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGtt                                                                                                                                           >  4:276987‑M2/57‑195 (MQ=255)

                                                                                                                                                                                                                          agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt                                                                                                                                       <  2:276987‑M2/143‑1 (MQ=255)

                                                                                                                                                                                                                              gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGACGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGAGACTGGAAGA‑GTGTTCCGGTAAAAGCCACTGAAGGGGTTGAAGGACGGGAGCACACCCTGGGGGAg                                                  >  3:678926‑M2/49‑275 (MQ=255)

                                                                                                                                                                                                                              gattgatcaaaaaagttaccgcactaggcccgtagattcgagaaggtaAGTGAAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGTAACCGGCCCCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCACGAATCCCGTGTTACCTACAGTTACCAGTTTGGTCTGTTAGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGTGGTGGTTGAACGACTGGAGCACAACCTgcgtagg                                                  >  2:338827‑M2/49‑272 (MQ=255)

                                                                                                                                                                                                                                   atcaaaaaatttaccgcactagggcgttatattcgtgaaggtaAGTGCAAAGTTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTc                                              <  3:477478‑M2/232‑1 (MQ=255)

                                                                                                                                                                                                                                   atcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTTAGTTTCa                                             >  2:442092‑M2/44‑275 (MQ=255)

                                                                                                                                                                                                                                           atgtcccgcactaggcccgtatattcgtgcaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTGCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAg                                      <  1:467392‑M2/239‑1 (MQ=255)

                                                                                                                                                                                                                                                                tattcgtgaaggtaAGTGCAAATATAATCGATTCGTTTTCGATTGTCGGGGTGTAGGCGTCAACGGGAAACCGGCACCGGGAGGGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTGTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACAATGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGc                <  2:604444‑M2/261‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTc                                                                                                    <  2:402637‑M2/177‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGACTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGCATCCCGTTTTAGCTACAGTTACCAGTTTGGGCTGTTCGATAAAGCAGGCGACTGGATGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAACACGCACTGCGTGAGTTTCACTAGAAGCCTCGTGAACTTCTGACGGCGCTg              >  1:804496‑M2/13‑275 (MQ=255)

                                                                                                                                                                                                                                                                   tcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGAt                                                                                                                              >  4:227018‑M2/12‑163 (MQ=255)

                                                                                                                                                                                                                                                                   tcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTGTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACAATGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTGCACGAGAAGCTGCGTGACCTGCTGAAGACGATGa             >  4:496586‑M2/12‑275 (MQ=255)

                                                                                                                                                                                                                                                                      tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATc          >  2:64576‑M2/9‑275 (MQ=255)

                                                                                                                                                                                                                                                                       gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAg                                                                                                                          <  2:227018‑M2/156‑1 (MQ=255)

                                                                                                                                                                                                                                                                        aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTg        >  3:579097‑M2/7‑275 (MQ=255)

                                                                                                                                                                                                                                                                        aaggtaAGTGCAAAGAGAATCGATTCTTTTTCGATTGTCGTGCTGGTGGCGTGAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCt         <  3:160261‑M2/268‑1 (MQ=255)

                                                                                                                                                                                                                                                                          ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCAAGAGAAGCTGCGGGAACTGCTGATGAAGCGGAATCTGaa      >  3:143764‑M2/5‑275 (MQ=255)

                                                                                                                                                                                                                                                                           gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCCAACCCTGCGTGAGTTTCACGAGAAGCTGCTTGAACGGGTGACGACGCTGAATCTgaat     >  3:435443‑M2/4‑274 (MQ=255)

                                                                                                                                                                                                                                                                              aGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTTAACGACTGGAGCACACCCTGCGTGAGTTTCATGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTg  >  2:314260/1‑275 (MQ=255)

TGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGG‑AGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGA‑GTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTG  >  NC_000913/258414‑258958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`