| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,776,167 | Δ1 bp | 10.9% | intergenic (+8/+4) | mtlR → / ← yibT | mannitol operon repressor/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,776,167 | 0 | C | . | 10.9% | 144.0 / 13.2 | 46 | intergenic (+8/+4) | mtlR/yibT | mannitol operon repressor/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base C (21/20); new base . (3/2); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
CCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCGGAACGCCTG > NC_000913/3776022‑3776314 | ccACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGc > 4:9481/1‑149 (MQ=255) ttGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGc < 2:38298/149‑1 (MQ=255) aCCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCct > 4:93661/1‑149 (MQ=255) tCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAGACGT‑TGCTTACttt < 1:100754/135‑3 (MQ=255) tCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAGACGT‑TGCTTACttt > 2:100754/1‑133 (MQ=255) tttGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAGACGT‑TGCTTACtt < 3:1773/129‑2 (MQ=255) tttGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCAGACGT‑TGCTTACtt > 4:1773/1‑128 (MQ=255) tGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGa < 1:60435/149‑1 (MQ=255) gcaatgcaaATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAg > 1:82617/1‑149 (MQ=255) aaaTTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCcgacga < 1:8909/149‑1 (MQ=255) cgcTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTc < 1:92843/149‑1 (MQ=255) aaCAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGt > 3:8208/1‑149 (MQ=255) aCAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATATTCCAGACGtt > 2:30694/1‑149 (MQ=255) ggCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTa < 1:47860/149‑1 (MQ=255) tgCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGatat > 4:52121/1‑149 (MQ=255) gATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTAc < 4:33634/97‑1 (MQ=255) gATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTAc > 3:33634/1‑97 (MQ=255) aTCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACg > 2:75768/1‑149 (MQ=255) aacaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCa > 3:84464/1‑149 (MQ=255) aacaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCa > 3:20729/1‑149 (MQ=255) acaaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCAc < 2:23019/149‑1 (MQ=255) aaTGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGc < 4:84464/149‑1 (MQ=255) tCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCa > 1:36704/1‑149 (MQ=255) actgaGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTcc < 2:25548/149‑1 (MQ=255) aGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCcggac > 2:87290/1‑149 (MQ=255) gCTGATTTTGGAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTc < 1:20787/143‑1 (MQ=255) tGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCcggacgga > 4:75642/1‑149 (MQ=255) ttCCAAAGTCAGCTTAAAACAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCGGCTTCGGCCGATAAAGCCGCCGATAATACTCAAGACGTTGTAGATATAACGGAACGCTTTCATCGGGTATTCCGGACGGAATcg > 2:98289/1‑149 (MQ=255) aaaaTCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGtt < 1:61194/149‑1 (MQ=255) gCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGgga < 1:57750/149‑1 (MQ=255) cTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGTCCGGAAtaccc < 2:60181/139‑5 (MQ=255) cTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGTCCGGAAtaccc > 1:60181/1‑135 (MQ=255) acaaaGCCTTTCAAAAGTAAGCA‑ACGTCTCCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGtt < 2:444/134‑1 (MQ=255) aaaaaGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGtt > 1:444/1‑136 (MQ=255) aaGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGtttt > 1:68596/1‑149 (MQ=255) aaGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGtttt < 4:27027/149‑1 (MQ=255) gCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGtttttt < 2:61547/149‑1 (MQ=255) gCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGtttttt > 2:15003/1‑149 (MQ=255) ccTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTAGCTGCTTCGGCCGATAAAGACGACGATAATAATCCAGACGTTGTAGATATAACGGCACGCTTTAATCGGGTATTTAGGACGGAATAGCGTTACGAGGAGGAAGTTTTTTc > 2:88083/1‑149 (MQ=255) tctaaaGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCGGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGAt > 4:7879/4‑149 (MQ=255) gcaGTAAGCAGACGT‑TGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCTGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACt > 1:47453/3‑149 (MQ=255) tAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCg > 1:100679/1‑149 (MQ=255) gCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCGGaa < 3:93661/149‑1 (MQ=255) gTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCGGAACGCCt < 3:9481/149‑1 (MQ=255) gTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCGGAACGCCt < 1:15003/149‑1 (MQ=255) tCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCGGAACGCCTg < 2:82617/149‑1 (MQ=255) | CCACAGTTTGAACCAGCAGACTCCAGTTTGTATGCAATGCAAATTCAGCGCTATCAACAGGCTGTGCGATCAACAATGGTCCTTTCACTGACTGAGCTGATTTCCAAAATCAGCTTAAAAAAAGCCTTTCAAAAGTAAGCA‑ACGTCTGCTTACTGCCCCTCTACCTGCTTCGGCCGATAAAGCCGACGATAATACTCCAGACGTTGTAGATATAACGGCACGCTTTCATCGGGTATTCCGGACGGAATCGCGTTACGGGGAGGAAGTTTTTTCAGATACTCCCGGAACGCCTG > NC_000913/3776022‑3776314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |