BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,005	T→A	D73V (GAT→GTT)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,005	0	T	A	99.5%	675.6 / NA	202	D73V (GAT→GTT)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (84/117); total (85/117)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 202 total aligned reads displayed.

AACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAT‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAG  >  NC_000913/4116735‑4117270

aaCTGCACTTGACCACGCCGTGACCGCTGTCGAGATCCTTCCACATGTTCGAGGATTCACTTCAATTTTGTG‑CCAGAAAAATAAGTGTCAATCATCAGACCGGTATTGCTGTGGATATAGTCTTCTAAACCGTCCCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCTCTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGa                                                                                                                                                                                                                                                                       <  4:1022171/275‑1 (MQ=255)

aaCAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGTTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑Cggca                                                                                                                                                                                                                                                                      >  4:486133/1‑274 (MQ=255)

 aCAGCAATTCACCACGACGTGCACGCTGGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGaa                                                                                                                                                                                                                                                                      <  1:338539/275‑1 (MQ=255)

     cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTg                                                                                                                                                                                                                                                                   >  1:228453/1‑274 (MQ=255)

       aTTCCCCACGATGTGCACGCTCGCGAGGGCCCTCCACATGGTCGAAGATCCACTTCACTTGTGTG‑CCAGAAAAGTACGGGTCAATCCCCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTANAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGatat                                                                                                                                                                                                                                                               <  4:567661/276‑1 (MQ=255)

             cacGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCt                                                                                                                                                                                                                                                          <  1:93768/275‑1 (MQ=255)

               cgacgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGGTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATATCGATAGCTGACATTTGAA‑CGGAACCGATATCTGCtt                                                                                                                                                                                                                                                         >  1:310682/1‑274 (MQ=255)

                  cgTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGcc                                                                                                                                                                                                                                                    >  2:993824/1‑276 (MQ=255)

                            cgcgAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACTCTGGTTCGTAATACCGATAGCTCCAA‑TTGATACGTAACTGATATCGGCTTTCGCCAGCACTTCTa                                                                                                                                                                                                                                          >  4:78113/1‑276 (MQ=255)

                                 gagCCTTCCACATGGTCGAGGATCCACTTCACTTTTGTG‑CCAGAAAAGCACGGGTCAAACACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATTGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCa                                                                                                                                                                                                                                                   <  2:902262/262‑1 (MQ=255)

                                    ctttCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCATACCGGTATGGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                                                                                                   <  2:464985/273‑1 (MQ=255)

                                    cgttCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                                                                                                   <  2:924934/273‑1 (MQ=255)

                                    ccttcccactGGTCGAGAGTCCCCTTCACTTGGGGT‑CCAAAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCTGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                                                                                                                                                                   <  3:945861/266‑1 (MQ=255)

                                    ccTTCAACATGGTCGAGGAGCCACTTCACTTTTGGG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                                                                                  <  2:379093/276‑1 (MQ=255)

                                     cTTCCACATGGTCGGGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                                                                                  <  1:894230/275‑1 (MQ=255)

                                       tCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCCGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCTCAGACAATAGTGGTTTCACGCTGGTTAGTTCTCCCGATAGCTGCATTTTGAA‑CGGAACTGATCTCGTCTTTCGCCATCCTTTTTACCAGCGTGGa                                                                                                                                                                                                                                >  2:632831/1‑275 (MQ=255)

                                              ggTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCTCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGCTGGTTGGTAATACCGATAGCTTCACTTTGAA‑CGGAACTGAATTGGTCTTTCGCCCTCCCTTCTACCAGCTGGGAGCTTTg                                                                                                                                                                                                                          >  3:967705/1‑274 (MQ=255)

                                               gTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTg                                                                                                                                                                                                                          >  1:797642/1‑273 (MQ=255)

                                                    ggATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGcc                                                                                                                                                                                                                   <  3:872070/275‑1 (MQ=255)

                                                     gATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATACCGGCTTTCGCCGGCCCTTCTACCAGCGTGGGTCTTTGGGTGGccc                                                                                                                                                                                                                  >  3:565774/1‑275 (MQ=255)

                                                       tCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCTGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATA‑CGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                              <  2:398902/276‑1 (MQ=255)

                                                        ccATTTCACGTTGGTGACCAG‑AAAGTACGTGTCAATCACCAGAACGGTATTGCTGCGGATATAAACGTCTAAACCGTCACGTTGTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAt                                                                                                                                                                                                              <  4:957143/276‑1 (MQ=255)

                                                          acttcacttTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCATAttt                                                                                                                                                                                                            >  2:121867/1‑276 (MQ=255)

                                                          acttcacttTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGACCTTCTCCCACCTTGTAGCTTTGGGTTGcctattttt                                                                                                                                                                                                            >  4:207330/1‑269 (MQ=255)

                                                          acttcacttTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATACTGGCTTTCGCCAGCCATTCTACCAGCCTGGCGCTTTTGGTGGCCCAGAtt                                                                                                                                                                                                             >  3:805553/1‑275 (MQ=255)

                                                          acttcacttTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGCCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGGGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTGGccccgttt                                                                                                                                                                                                             >  3:382280/1‑270 (MQ=255)

                                                                    ggTG‑CCATAAAAGTACGGGTCAATCACCAGACCGTTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                  <  2:320556/276‑1 (MQ=255)

                                                                    ggTG‑CCAGAAAAGTACGGGTCCATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                  <  2:132605/276‑1 (MQ=255)

                                                                    ggTG‑CAGGAAAAGTATGGGTCAATCATCCGTACGGTATTGGTTCGGATATAATCTTATAAGCCGTCACGTTTTAAATGCTCTGAGGTTTCTGCGGGACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                  <  4:264227/276‑1 (MQ=255)

                                                                     ggg‑CCAGAAAAGTACGGGTCAATCCCCAGTCCGGTTTTGCTGCGGATATAATCGTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                  <  3:116370/273‑1 (MQ=255)

                                                                      tG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATCTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGGTTCTTTTTCCCATTCCATTTGGGTTTCACACCGGTTCGTAATACCGATAGCCGCAACTTTTA‑CGGCACTGAAATCGGCTTTCGCCAGTACTTCTCCAGGCGTGGAGCTTTGGGTGGCCCAGATTCCCATTGGGt                                                                                                                                                                                                   >  3:336304/1‑273 (MQ=255)

                                                                                aGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                                                                                                                                                                        <  1:715785/275‑1 (MQ=255)

                                                                                     gggTCAATCACCATACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATATCTGCGGTACGACGGAACGGCCAGACAATGGCGTTATAGATAGGCTGGCCGGTTTCTTTTTCCCAGACCATAGTGGTTTCACGCTGGTTCGTACTACCGATAGCTGCAATTTGCA‑CAGAACTGATATCGCCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                                                                                                                                                                    <  3:677048/274‑1 (MQ=255)

                                                                                       gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTTGCCAGCACTTCTACCCGCGTTGAGCTTTGGGTGGCCCAGCTTTACCTTTGGTTCTGTTTGTCCCAATCTgg                                                                                                                                                                                >  4:646464/1‑276 (MQ=255)

                                                                                       gTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTgg                                                                                                                                                                                >  2:394186/1‑276 (MQ=255)

                                                                                           ctagccAGACCGGTAGTGGTGCGGATATGATCTTCTAAGCCGTACGGTTTTGAATGCTCGCATGTTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGGCAATAGTGGTTTCACTCTGGTTCGTAATACCTATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTGTCGCCAGCACTTCTACCGGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                                                                                                                                                              <  4:719912/270‑1 (MQ=255)

                                                                                            tCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                                                                                                           >  2:341420/1‑276 (MQ=255)

                                                                                                 aGACCGGTATTGCTGCGTATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                                                                                                                                                         <  1:464425/273‑1 (MQ=255)

                                                                                                 aGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCATACAATATTGGTTTCACGCTGGTTCGTAATACCGATATCTGAAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCCCTTCTACCAGCGTGTAGCTTTGGGTGGCCCAGCATTCCATTGGGTCGTGTTCTACCCAACCTGGGTTTTGGTa                                                                                                                                                                       >  2:172143/1‑275 (MQ=255)

                                                                                                     cGGTATTGCTGCGGATATAATCTGCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                                                                                                                                                                  <  4:497071/212‑1 (MQ=255)

                                                                                                        tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTTCGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGCTAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGTTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTGCCCAACATGGTTTTCGGTAGTTTTgt                                                                                                                                                                >  1:543191/1‑274 (MQ=255)

                                                                                                        tATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                                                                                                                                                                  >  1:105673/1‑241 (MQ=255)

                                                                                                                gggAGATAATCTGCTAAACCTGCACGTTTGAATTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                                                                                                                                                       <  2:160281/275‑1 (MQ=255)

                                                                                                                 ggATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAAATGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGTTGTCCTAGATTTCCATTGGGTCGTGTTCTAACCATCGTGTTTTTGGGTTGTTTTGCTAATATTcgc                                                                                                                                                      >  4:884091/1‑276 (MQ=255)

                                                                                                                  gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGCTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGTTAGATTTGCTCAAATTcga                                                                                                                                                      >  1:172256/1‑274 (MQ=255)

                                                                                                                  gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGTTAGTTTTGCTCAAAtttgg                                                                                                                                                      >  3:185755/1‑272 (MQ=255)

                                                                                                                  gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTTGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCCAAATTcgc                                                                                                                                                      >  1:966051/1‑275 (MQ=255)

                                                                                                                  gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt                                                                                                                                                                                           >  4:237255/1‑238 (MQ=255)

                                                                                                                  gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcgc                                                                                                                                                      >  3:414342/1‑275 (MQ=255)

                                                                                                                  gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGGGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCGCTAATTcgcg                                                                                                                                                     >  2:274398/1‑276 (MQ=255)

                                                                                                                        atatgctAAACCGTCCTTGTTTAAAGGGTAGCATATTTCTGGGTTACGACGTCACTTCAAGACACTGGCGTTATAGATAGGCTTGCCGGTTTTTTTTTCCCAGACAATAGTGGTTTCACGCGGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTCTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                               <  4:277952/271‑1 (MQ=255)

                                                                                                                        aTCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATTGCGTTATAGATAGTCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                               <  2:496052/276‑1 (MQ=255)

                                                                                                                         tcttctAAACCGTCACGTTTTACATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                                                                                              <  2:1037279/276‑1 (MQ=255)

                                                                                                                            tctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                                                                                                           <  4:915704/276‑1 (MQ=255)

                                                                                                                             ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCACTTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGGTTTGGGTGGCCCAGATTTCCAGTGGGTCGTGTTCTACCCAACCTGGATTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACg                                                                                                                                           >  1:143714/1‑275 (MQ=255)

                                                                                                                                aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                        <  2:560272/275‑1 (MQ=255)

                                                                                                                                 aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCAGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                                        <  1:447519/274‑1 (MQ=255)

                                                                                                                                     tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGTTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGat                                                                                                                                   >  2:167069/1‑275 (MQ=255)

                                                                                                                                     tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGGGACACGCTAATGa                                                                                                                                    >  1:438637/1‑274 (MQ=255)

                                                                                                                                          ttttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                                                                                              <  3:830830/275‑1 (MQ=255)

                                                                                                                                                 tgcttgacgATTGCGGCGGGAAGACGGCACTGCCAGACAATGGCGTTATAGCTAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTGCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                      <  4:767939/268‑1 (MQ=255)

                                                                                                                                                  gCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                                      <  1:26604/275‑1 (MQ=255)

                                                                                                                                                     cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTAGTAATACAGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGCGCTTGGGGTGGCCCAGATTTCAGTTGGGTCGTGTTCGAGCCAACCTGTTTTTGGTTAGATTTGCTCAAATTCGCGGTCTGACAAGCTAATGATATTTGCAtatgttcaca                                                                                                                  >  4:1037813/1‑267 (MQ=255)

                                                                                                                                                            atttGCGGTACGACGGCACTGCCAGACAATGGCGTTAGAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATACTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                                           <  2:7959/273‑1 (MQ=255)

                                                                                                                                                               tGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACCCGCTTAGTGTCTTGGCATCGTGATCCATTACTACcga                                                                                                        >  4:290073/1‑275 (MQ=255)

                                                                                                                                                                    tACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGCGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCgg                                                                                                    >  2:850095/1‑275 (MQ=255)

                                                                                                                                                                        acgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTGCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                <  2:207330/275‑1 (MQ=255)

                                                                                                                                                                                           gCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAg                                                                             <  1:389120/275‑1 (MQ=255)

                                                                                                                                                                                            ggTTATAGGTAGGCTTGCCGGTTTCTTTTGCCCCAGCCATAGTGACTGCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTATACCAGCGTGGCGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCGACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                                           <  4:393101/275‑1 (MQ=255)

                                                                                                                                                                                               tataGATAGGCTTGCCGGTTTCTTTTTACCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGt                                                                                                                                                                                                                       >  1:410802/1‑133 (MQ=255)

                                                                                                                                                                                                atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                                         <  1:189534/274‑1 (MQ=255)

                                                                                                                                                                                                      agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                                >  3:472269/1‑245 (MQ=255)

                                                                                                                                                                                                          tttccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                                            <  1:472271/242‑1 (MQ=255)

                                                                                                                                                                                                           tGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAAAGATAtttttt                                                             >  1:246755/1‑275 (MQ=255)

                                                                                                                                                                                                                     tttttCCCCGAAAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGTGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCGACGATATATTTTTTTTCAGTc                                                    <  1:613260/274‑1 (MQ=255)

                                                                                                                                                                                                                      ttttCCCAGACGATAGTGGTTTCCCTCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGTAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATg                                                 <  4:147035/276‑1 (MQ=255)

                                                                                                                                                                                                                            cAGACAATATTGGTTTCACGATTGTTCGTAATACCGATAGCTACAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATACATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                            <  3:783983/275‑1 (MQ=255)

                                                                                                                                                                                                                              gaaaaTATTGGTTTTAAGCTGGTTCGTAATACCGTATGCTGGAAATTGAA‑CGGAACTGATATCGGACTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                                          <  4:167069/272‑1 (MQ=255)

                                                                                                                                                                                                                                 aaTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTc                                       <  1:855587/275‑1 (MQ=255)

                                                                                                                                                                                                                                   aaGTCGTTTCACGCTGTTTGATCATAACGTCAGCAATAAATTGAA‑CGGAACTGATATCGGGTTTCGCATCCACTTCTACAAGGTGGGCACTTTGTGTGGCCCAGATGTCCGTTGGGTCGTGTTCTACCCAACATGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGGGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                                    <  4:501539/275‑1 (MQ=255)

                                                                                                                                                                                                                                    aggggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTATACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                   <  2:391704/273‑1 (MQ=255)

                                                                                                                                                                                                                                    aGTGGTTTCACTCTGGTTCGTAAGACCGTTAGCTGCAATTTGAA‑CGGAACTGTTATCGGCTTTCGCCAGGACGTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                   <  4:427053/276‑1 (MQ=255)

                                                                                                                                                                                                                                    aGTGGTTTCACGCTGGTTCGTAATACCGATGGCTGCAATTGGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                   <  2:451370/276‑1 (MQ=255)

                                                                                                                                                                                                                                     gTGGTTTCACTCTGGGTCGTAATACCGATAGCTGCAATGTGAA‑CGGAACTGATGTCGGCTTTCTCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGGGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                   <  3:199931/275‑1 (MQ=255)

                                                                                                                                                                                                                                     gTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                                   <  3:244046/275‑1 (MQ=255)

                                                                                                                                                                                                                                     gTGGTTACTAGCTGGGTCGTAATAGCCAGAGCTGAAATTTGAA‑AGGACCTGTTAGCGGCTTTCGCCAGCACGTCTACCAGCTTGGAGCTTTGGGTGGACCAGATTTCCATTGGGTTGTGTTCTACCGAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGAGACACGCTAATGATCGTGGCATCGTGATCCATTACGATCTCGCGGGGGCTGGGGGTGCCCTGGTCGAGCGCAACGATATATTTGTTTTCAGTCATGTTTAATTGTCCCGt                                   <  3:543189/275‑1 (MQ=255)

                                                                                                                                                                                                                                      tGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                 <  2:44693/276‑1 (MQ=255)

                                                                                                                                                                                                                                       ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                                 <  1:755434/275‑1 (MQ=255)

                                                                                                                                                                                                                                           tCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCTTCGTGATCCATTACGACCGCGCGGGAGCGGGTGGTGTCCTGGTCGAGCGCAACGGTATATTTTTTTTCCGTCATGTTTAATTTTACTGTAgcgttc                            >  4:329964/1‑271 (MQ=255)

                                                                                                                                                                                                                                                  ggTTCGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTGGTCATATTACAg                      >  1:514492/1‑275 (MQ=255)

                                                                                                                                                                                                                                                      cGTAATACCGATAGCTGCAATTTGAA‑CGGTACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGaa                  <  2:290073/275‑1 (MQ=255)

                                                                                                                                                                                                                                                      cGTAATACCGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGata                                                                   <  2:726193/226‑1 (MQ=255)

                                                                                                                                                                                                                                                           tcacGATAGCTGCAAATTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGTACCAGATTTCCTTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt             <  1:59697/272‑1 (MQ=255)

                                                                                                                                                                                                                                                             ccGATAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCCCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTTTCCCGTAGTCATGTTACAGCGACGtttttt           >  1:996772/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                aTAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCa                                                                                                                                                             >  3:395579/1‑126 (MQ=255)

                                                                                                                                                                                                                                                                aTAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGTCATCGTTATCCATTACGACCGCGCGGGCGCTGGTGGTGCCCTGGTCGAGCGCAACGCTATATTTTTTTTCATTCGTGTTTATTTTTCCCGTAGTCATATTACAGCGAAGCTTCTTGttt       >  2:961466/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                aTAGCTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAt                                           >  3:351456/1‑240 (MQ=255)

                                                                                                                                                                                                                                                                    cTGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                               <  1:36041/136‑1 (MQ=255)

                                                                                                                                                                                                                                                                     tGCAATTTGAA‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCCTCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCCGGTCGAGGGGAACGATATATTTTTTTTCCGCAGTGTTTAATTGTCCCGTAGCCATTTTACAGCGAAGCTTTTTTTTCtat    >  3:891289/1‑272 (MQ=255)

                                                                                                                                                                                                                                                                      gCAATTTGAA‑CGGATCGGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAg  <  2:646464/275‑1 (MQ=255)

AACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG‑CCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGAT‑CGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAG  >  NC_000913/4116735‑4117270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`