| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,231,963 | T→A | 5.2% | Y260N (TAT→AAT) | umuC → | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,231,963 | 0 | T | A | 5.2% | 332.8 / 4.4 | 96 | Y260N (TAT→AAT) | umuC | translesion error‑prone DNA polymerase V subunit; DNA polymerase activity |
| Reads supporting (aligned to +/- strand): ref base T (48/43); new base A (2/3); total (50/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTC > NC_000913/1231817‑1232111 | tCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTAt > 3:116843/1‑149 (MQ=255) gTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAAGTGCGCGGCGAAAATTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGt < 2:504999/149‑1 (MQ=255) gTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGt < 2:854643/149‑1 (MQ=255) gTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGt < 3:614024/149‑1 (MQ=255) tttATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTc < 4:322888/149‑1 (MQ=255) tATCCGTAAACAATTTAATGTCGTGCTCGAAAGAACGGTGCGTCAACTGCGCGGCGAAACCCGTTTGCAACTCGAAGTTTTTGCACCGACTAAGCAGGAAATTATCTGTTCCCGCTCGTCTGGTGAACGCATCGACGGATAATCCGtga < 4:581213/149‑3 (MQ=255) aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGAt < 3:706881/149‑1 (MQ=255) aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGAt > 4:32991/1‑149 (MQ=255) tCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATg > 3:275708/1‑149 (MQ=255) cGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTCTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATAATCCGT‑GATGCgt < 4:755816/149‑2 (MQ=255) gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCgg > 3:3590/1‑149 (MQ=255) gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCgg > 4:782343/1‑149 (MQ=255) gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGATGAACGCATC‑ACGGATTATCCGTCGATGCgg > 2:21362/1‑149 (MQ=255) tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGT‑GATGCgtt > 1:134638/1‑147 (MQ=255) aaaCATTTTAATGTCGTGCTCGAAAGAGCGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCa < 1:380510/149‑1 (MQ=255) aaaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGT‑GATGCgttc < 2:134638/149‑4 (MQ=255) aaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAg > 1:115350/1‑149 (MQ=255) aaCATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAg > 4:27829/1‑149 (MQ=255) gTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAg > 1:27374/1‑149 (MQ=255) tCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGt > 4:113231/1‑149 (MQ=255) gTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTa > 2:414482/1‑149 (MQ=255) tGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTAc < 4:401664/149‑1 (MQ=255) tCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt > 4:300356/1‑149 (MQ=255) gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc < 1:564587/149‑1 (MQ=255) gAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc > 3:698260/1‑149 (MQ=255) aaaGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGcc < 3:395778/149‑1 (MQ=255) aaaGAACGGTGCGAGAACTGCGCGGCGAACCCTGTTTGCAACTGGGAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGACGCGGCAGGCCATTTGTAGTTACGCTGcc > 2:802297/1‑149 (MQ=255) aaGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGccc > 3:762040/1‑149 (MQ=255) cGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcg > 3:124570/1‑149 (MQ=255) gTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcggc < 4:714498/149‑1 (MQ=255) gTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcggc < 2:115350/149‑1 (MQ=255) tGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCggg > 3:85217/1‑140 (MQ=255) tGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCggg < 4:85217/140‑1 (MQ=255) aaCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa > 1:478100/1‑149 (MQ=255) aaCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaaa > 1:109856/1‑149 (MQ=255) gcgcGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTc < 3:238855/149‑1 (MQ=255) gcggcgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGc > 1:119293/1‑149 (MQ=255) cggcgAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCa < 4:275708/149‑1 (MQ=255) aaCCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGAAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAg < 3:142114/149‑1 (MQ=255) cccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCa < 2:564553/149‑1 (MQ=255) cccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCa > 1:265408/1‑149 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAt > 2:691181/1‑149 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAt > 4:85017/1‑149 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAt > 3:110274/1‑149 (MQ=255) ccTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAt > 4:25641/1‑149 (MQ=255) tGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCa > 3:110083/1‑149 (MQ=255) tttGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAat < 2:109856/149‑1 (MQ=255) gCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATAtt < 3:236158/149‑1 (MQ=255) aCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc < 3:32991/149‑1 (MQ=255) aCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc > 2:42292/1‑149 (MQ=255) aCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc > 4:853092/1‑149 (MQ=255) gtagagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCAGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGAAGCGAGCATCAATATTGTCgggt > 4:270472/3‑147 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt < 3:658790/149‑1 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt < 2:314711/149‑1 (MQ=255) gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt > 2:581932/1‑149 (MQ=255) aaGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt < 3:27829/149‑1 (MQ=255) aaGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt < 2:43101/149‑1 (MQ=255) agTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAtc < 3:271186/149‑1 (MQ=255) tttGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAtctc > 2:649474/1‑149 (MQ=255) ttGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTcc < 4:678827/149‑1 (MQ=255) cACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACg > 3:869401/1‑149 (MQ=255) aCCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc < 3:249669/134‑1 (MQ=255) aCCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc > 4:249669/1‑134 (MQ=255) cgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTttatt < 2:27374/149‑1 (MQ=255) aGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa > 2:375311/1‑148 (MQ=255) aGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa < 1:375311/148‑1 (MQ=255) gCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGa < 2:549894/149‑1 (MQ=255) cAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGAc < 1:539606/149‑1 (MQ=255) gAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTc > 3:456098/1‑149 (MQ=255) aaaTTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCa < 2:265408/149‑1 (MQ=255) aaTTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCAc < 4:124570/149‑1 (MQ=255) tCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGCCTGGCCCGTGGTAATTACGGTGCCCCGGAGGCGGAAACGCTACGCAGCGAGCATCAATATTGTCGGTTTGTCGCCGCGCATATTAAGACGTCACCAtta > 2:203803/1‑148 (MQ=255) tGTTCACGCTCGTTTGGAGAACGAATC‑ACGGATTATCTGTCGATGCGGCTGGCCATTTGTAGTTACGCTGCCGGGGCGGCGGAAAAACTTCGCAGCGCGCATCGATATTTTCGGTTTATCTCCACGTTTATTAATACGTCACCAGTTgc > 2:590814/1‑149 (MQ=255) ccGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTATGACGTCACCATTTGCGCTCa > 3:389326/1‑149 (MQ=255) cGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTGTTAAGACGTCACCATTTGCGCTCaa < 2:119293/149‑1 (MQ=255) cGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCaa > 4:139327/1‑149 (MQ=255) cGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCaa < 2:478100/149‑1 (MQ=255) gCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAAt > 1:181052/1‑149 (MQ=255) cGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGaa > 3:682439/1‑149 (MQ=255) gTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc < 3:382532/149‑1 (MQ=255) gTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc < 4:110083/149‑1 (MQ=255) gTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc < 4:110274/149‑1 (MQ=255) gTTTGGTGAACGCATC‑ACGGATTATCCGGCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAc < 2:712676/149‑1 (MQ=255) tGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTatg > 3:663877/1‑48 (MQ=255) tGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACg < 4:663877/50‑1 (MQ=255) cATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCa > 3:549768/1‑149 (MQ=255) atcgaCGGATAATCCGT‑GATGCGTCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCaa > 1:185283/5‑149 (MQ=255) aTC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCaa < 1:649474/149‑1 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAAt > 3:173603/1‑149 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAAt < 4:762040/149‑1 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAAt > 4:830797/1‑149 (MQ=255) tC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAAt > 2:438815/1‑149 (MQ=255) c‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATa < 4:682439/149‑1 (MQ=255) aTTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa > 1:855609/1‑103 (MQ=255) aTTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa < 2:855609/103‑1 (MQ=255) aTTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGcgcg > 4:388605/1‑149 (MQ=255) tATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTc > 4:151945/1‑149 (MQ=255) | TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATC‑ACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTC > NC_000913/1231817‑1232111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |