| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,799,635 | T→A | 10.0% | intergenic (+22/+15) | alaE → / ← ygaC | alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,799,635 | 0 | T | A | 10.0% | 256.1 / 15.5 | 80 | intergenic (+22/+15) | alaE/ygaC | alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base T (35/37); new base A (6/2); total (41/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAG > NC_000913/2799487‑2799783 | tCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑At > 1:223456/1‑149 (MQ=255) gATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGc > 1:546693/1‑149 (MQ=255) ttATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCa > 3:838157/1‑149 (MQ=255) tGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTc < 2:149449/149‑1 (MQ=255) gcggcgGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTcgc > 3:181677/1‑149 (MQ=255) ggcggTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGt < 2:675716/149‑1 (MQ=255) gcggTCAGTTCAAACATCGGTGTGTCGATGTTGATGGGGGCGGTTTAGGGCTACTTCCTCGATTATTGCCTCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTACAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTc < 3:16974/149‑1 (MQ=255) ggTCGGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCtt > 4:539583/1‑149 (MQ=255) ggTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCtt < 2:7783/149‑1 (MQ=255) ggTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCtt < 3:146508/149‑1 (MQ=255) ggTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCtt < 4:321387/149‑1 (MQ=255) tCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑GATGCCAGTcg < 3:591275/141‑1 (MQ=255) tCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑GATGCCAGTcg > 4:591275/1‑141 (MQ=255) cAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTAAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTaa < 4:63354/149‑1 (MQ=255) aGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAAc < 3:883232/149‑1 (MQ=255) ttCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATTGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTTAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑GATGCCAGTctc > 1:911068/1‑136 (MQ=255) ttCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGCGAT‑GCCAGTcgc < 2:911068/138‑1 (MQ=255) aaCATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCtt > 1:320360/1‑149 (MQ=255) aCAGCGGTGTTTCGATGTTGATGGGGGCGGTTTTTGGCTACTGCCTCGCTGCGGGGCGCCGACTGTTTTCAGGCTGTGGTTGCGAGCAGGCAACAGCATGGGGCTGTTTTTCGCGACGGGC‑GTCACCAGTTGCTCCTTTGACTGGCTTc > 4:676535/1‑149 (MQ=255) ttgttTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGACGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCaaa > 2:567553/1‑149 (MQ=255) ttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTc > 1:814286/1‑121 (MQ=255) ttCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTc < 2:814286/121‑1 (MQ=255) tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc < 4:916471/149‑1 (MQ=255) tCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAAc < 2:47184/149‑1 (MQ=255) cGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGt > 2:146123/1‑118 (MQ=255) cGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGt < 1:146123/118‑1 (MQ=255) gATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAAGTTGCTTCGCCAAATAAACCa > 3:760436/1‑149 (MQ=255) gATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCa > 4:95268/1‑149 (MQ=255) gATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCaaa < 2:21133/149‑1 (MQ=255) ggggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAAc < 2:543396/149‑1 (MQ=255) gggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCtt > 1:391683/1‑114 (MQ=255) gggCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCtt < 2:391683/114‑1 (MQ=255) gCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGtt > 3:354798/1‑149 (MQ=255) cGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTc < 4:762755/149‑1 (MQ=255) gTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCaa > 3:693303/1‑149 (MQ=255) aTGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAgcgc < 1:567553/149‑1 (MQ=255) tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAacac > 1:807614/1‑149 (MQ=255) tACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAacac > 4:453360/1‑149 (MQ=255) tCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGa < 1:848606/149‑1 (MQ=255) cTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAAc > 4:217034/1‑149 (MQ=255) tCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACt > 2:495867/1‑149 (MQ=255) gATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTaa < 4:181677/149‑1 (MQ=255) ttattGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAg < 2:320360/149‑1 (MQ=255) tGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAcc > 4:35163/1‑149 (MQ=255) tttAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGt > 4:84893/1‑149 (MQ=255) tAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCgg < 2:772653/139‑1 (MQ=255) tAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCgg > 1:772653/1‑139 (MQ=255) aaaGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAAt > 2:250127/1‑149 (MQ=255) tCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTc < 1:495867/149‑1 (MQ=255) agcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGcc < 3:217034/149‑1 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCt > 2:254778/1‑149 (MQ=255) gcagGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCt > 2:563800/1‑149 (MQ=255) agGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGc < 3:539583/149‑1 (MQ=255) ggTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa < 1:80591/71‑1 (MQ=255) ggTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATaaa > 2:80591/1‑71 (MQ=255) ggTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCt < 4:354798/149‑1 (MQ=255) tAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTaa > 1:263649/1‑149 (MQ=255) aaaaGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTaaa < 2:546693/149‑1 (MQ=255) aaaGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAAt > 4:847593/1‑149 (MQ=255) aaGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATa > 2:94901/1‑149 (MQ=255) aaGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATa < 4:693303/149‑1 (MQ=255) ccTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATagag < 1:250127/149‑1 (MQ=255) gACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCgg > 2:231175/1‑149 (MQ=255) aCTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGa < 2:202787/149‑1 (MQ=255) ttcttTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt < 2:263649/149‑1 (MQ=255) ttcttTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAAtt > 1:327051/1‑149 (MQ=255) ttttCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCt < 3:84893/149‑1 (MQ=255) ttCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTgg > 1:920901/1‑149 (MQ=255) ttCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTgg > 1:822453/1‑149 (MQ=255) acgcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAc > 4:238042/2‑101 (MQ=255) acgcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATAc < 3:238042/100‑1 (MQ=255) gcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa > 3:623013/1‑149 (MQ=255) gcgACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTaa > 1:450140/1‑149 (MQ=255) gACTGGC‑GATGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATg > 1:519339/1‑149 (MQ=255) gACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATg > 4:758273/1‑149 (MQ=255) aCTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGa < 1:518857/149‑1 (MQ=255) aCTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGa < 1:254778/149‑1 (MQ=255) tGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc > 3:303739/1‑149 (MQ=255) tGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATc < 2:223456/149‑1 (MQ=255) gC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc > 1:781203/1‑136 (MQ=255) gC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGc < 2:781203/136‑1 (MQ=255) tCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAg > 2:936532/1‑149 (MQ=255) | TCAGATTATGGCGGCGGTCAGTTCAAACATCGTTGTTTCGATGTTGATGGGGGCGGTTTATGGCTACTTCCTCGATTATTGCCGCCGACTGTTTAAAGTCAGCCGTTACCAGCAGGTAAAAGCCTGACTCTTCTTTTCGCGACTGGC‑ATCGCCAGTCGCGTCTTTAACTGGCTTCGCCAAATAAACCATTCAAATAACGTTCAAGCGCAACACGCGAACTAAAGCCATGCGGGATACCGTAATGCTCGTGTCGCTCGCCTGCTAAATAGAGCGGAAATTGCTGGTAATGATCGCAAG > NC_000913/2799487‑2799783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |