BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,330,402	Δ1 bp	5.9%	intergenic (+21/+15)	sohB → / ← yciN	inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,330,402	0	A	.	5.9%	755.4 / 36.2	219	intergenic (+21/+15)	sohB/yciN	inner membrane protein, S49 peptidase family protein/DUF2498 protein YciN
Reads supporting (aligned to +/- strand): ref base A (108/95); new base . (3/10); total (112/107)
Fisher's exact test for biased strand distribution p-value = 4.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

Only 100 of 219 total aligned reads displayed.

GGATATTGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGATGGGTTGTGTCTCTTTGTTCATTATTT  >  NC_000913/1330138‑1330673

      tGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCttt                                                                                                                                                                                                                                                                  >  2:13903/1‑275 (MQ=255)

             gTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCATCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAGACGCGAGG‑CTACGACCTCGCGTTTTGCTTTACTCAAc                                                                                                                                                                                                                                                           >  2:499478/1‑275 (MQ=255)

                   aCGGGTGAACACTGGTACGGACGACAGGCGGTATAGAAAGGCCTGGTATATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTTATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAGCCAgatg                                                                                                                                                                                                                                                     <  4:173053/275‑1 (MQ=255)

                               tGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa                                                                                                                                                                                                                                         >  2:479683/1‑275 (MQ=255)

                                       aaaacaGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcaca                                                                                                                                                                                                                                  <  2:566055/272‑1 (MQ=255)

                                                        aagagctgtgtgatgaAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTTACCGCTTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACTCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGGGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGa                                                                                                                                                                                                                  <  4:499478/263‑1 (MQ=255)

                                                        aaGGCCTGGTTGATGAAATCAACACAAGGTATGAAGTGTTTCTTAGCCTGATGGAATGCCGTGAAGTGGTCAATGTACGCTATTTGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                                                                                                                                                <  4:492135/275‑1 (MQ=255)

                                                          ggCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGca                                                                                                                                                                                                                                    <  2:236642/253‑1 (MQ=255)

                                                            ccTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACata                                                                                                                                                                                                             >  2:332317/1‑274 (MQ=255)

                                                                          tgaaaacCAGGTATGAAGTTATGCTTAGCGTGATGGAAGGCCGTTAAGTGGTCAATGTAAGCGATATGCAGCGTAAACGACTCATTGACCGATTCACAGGCAGCGCGGCAGAGAGAGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATGTATCTGAAAGCACATGGGCCa                                                                                                                                                                                                                           <  1:13507/241‑1 (MQ=255)

                                                                                   gTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCACAAGCCCTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATAACTCAGATGATATTTTTCAGAAAGCCCTTGGGCCAGGTGTTTGAACCTTTTAAACCTCCGC‑GTGATTTTGGCt                                                                                                                                                                                    >  4:528362/1‑275 (MQ=255)

                                                                                   gTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCt                                                                                                                                                                                    >  1:16647/1‑275 (MQ=255)

                                                                                    tgatgaAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTg                                                                                                                                                                                   >  2:468735/1‑275 (MQ=255)

                                                                                              aTTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGCGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGATTTTGGCTGTCGGAAAAcc                                                                                                                                                                         >  4:143765/1‑275 (MQ=255)

                                                                                                    aGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGtt                                                                                                                                                                    >  2:534675/1‑274 (MQ=255)

                                                                                                                    gTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCACTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTTCTTTTGGCTGTTGGCAATCCTTGTTCATCAAAAAAgtcgtc                                                                                                                                                    >  4:487913/1‑274 (MQ=255)

                                                                                                                     tGAAGTGGTCAATGTACACTATATACAGCGAAAACTACTCATTGACCGATTCAACGGCAGGGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGc                                                                                                                                                  <  1:90089/275‑1 (MQ=255)

                                                                                                                            gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGt                                                                                                                                                                                                                                                                          <  5:74222/149‑1 (MQ=255)

                                                                                                                            gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGGTCTTAG‑CCTCGCGt                                                                                                                                                                                                                                                                          <  6:13417/149‑1 (MQ=255)

                                                                                                                            gTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGt                                                                                                                                                                                                                                                                          >  6:252785/1‑149 (MQ=255)

                                                                                                                              cAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGttt                                                                                                                                                                                                                                                                        <  7:804321/149‑1 (MQ=255)

                                                                                                                               aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt                                                                                                                                                                                                                                                                       <  5:297443/149‑1 (MQ=255)

                                                                                                                               aaTGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGtttt                                                                                                                                                                                                                                                                       <  5:772757/149‑1 (MQ=255)

                                                                                                                                  gTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGCAAAACGCGAGGTCTTAG‑CCTCGCGtttgtct                                                                                                                                                                                                                                                                    <  5:97603/149‑5 (MQ=38)

                                                                                                                                     cGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTGAAAGACAAACGCGAGGTCTTAG‑CCTCGCGtttgtctttt                                                                                                                                                                                                                                                                 <  8:894421/149‑8 (MQ=255)

                                                                                                                                     aggtataTGAAGGGTAAACAACTCATTGACCTATTCACCGGAAGGGCGGCAGAGAGCGCCGATTGAGTGTTGCTACGCGGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCa                                                                                                                                   <  4:241571/271‑1 (MQ=255)

                                                                                                                                      gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTaa                                                                                                                                                                                                                                                                <  5:484103/149‑1 (MQ=255)

                                                                                                                                      gCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTaa                                                                                                                                                                                                                                                                <  8:878812/149‑1 (MQ=255)

                                                                                                                                          tatGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGa                                                                                                                                                                                                                  <  4:134445/191‑1 (MQ=255)

                                                                                                                                           atGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCCCCGGTAAATCCCAGCACGccct                                                                                                                             >  4:465443/1‑272 (MQ=255)

                                                                                                                                              cAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAg                                                                                                                                                                                                                                                        >  5:858654/1‑149 (MQ=255)

                                                                                                                                                gCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTTTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCCCCGGTACATCCCCGCACCCAATTACGc                                                                                                                        >  4:82847/1‑274 (MQ=255)

                                                                                                                                                 cGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt                                                                                                                                                                                                                    >  2:356944/1‑182 (MQ=255)

                                                                                                                                                  gTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTg                                                                                                                      >  4:42061/1‑274 (MQ=255)

                                                                                                                                                    aaaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGataata                                                                                                                                                                                                                                                  <  6:858654/149‑1 (MQ=255)

                                                                                                                                                     aaCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                                                                                                                                                <  4:356944/182‑1 (MQ=255)

                                                                                                                                                      aCGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtt                                                                                                                                                                                                                                                <  5:616696/149‑1 (MQ=255)

                                                                                                                                                           tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                                                                                                                                                           <  8:788628/149‑1 (MQ=255)

                                                                                                                                                           tCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCt                                                                                                                                                                                                                                           >  6:995688/1‑149 (MQ=255)

                                                                                                                                                             aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa                                                                                                                                                                                                                                         >  8:289568/1‑149 (MQ=255)

                                                                                                                                                             aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGa                                                                                                                                                                                                                                         >  6:351046/1‑149 (MQ=255)

                                                                                                                                                             aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAg                                                                                                                                                                                                                                      >  2:697380/1‑152 (MQ=255)

                                                                                                                                                             aTTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTGTCTTTAATCAACCAGATGATATTTTTCTGa                                                                                                                                                                                                                                         >  7:437835/1‑149 (MQ=255)

                                                                                                                                                               tGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCCGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGAGCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa                                                                                                                                                                                                                                       >  5:900601/1‑149 (MQ=255)

                                                                                                                                                               tGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGaaa                                                                                                                                                                                                                                       >  6:453854/1‑149 (MQ=255)

                                                                                                                                                                   cGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcac                                                                                                                                                                                                                                   >  6:172757/1‑149 (MQ=255)

                                                                                                                                                                    gATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGcaca                                                                                                                                                                                                                                  <  5:172757/149‑1 (MQ=255)

                                                                                                                                                                       tCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATgg                                                                                                                                                                                                                               <  6:724483/149‑1 (MQ=255)

                                                                                                                                                                         aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                                                                                                                                                           >  5:107245/1‑119 (MQ=255)

                                                                                                                                                                         aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAAc                                                                                                                                                                                                                                                           <  6:107245/119‑1 (MQ=255)

                                                                                                                                                                         aCCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGc                                                                                                                                                                                                                             >  6:188348/1‑149 (MQ=255)

                                                                                                                                                                           cGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTg                                                                                            <  2:42061/275‑1 (MQ=255)

                                                                                                                                                                              cAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGgt                                                                                                                                                                                                                        <  5:157622/149‑1 (MQ=255)

                                                                                                                                                                                  gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt                                                                                                                                                                                                                    >  8:529814/1‑149 (MQ=255)

                                                                                                                                                                                  gcgGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt                                                                                                                                                                                                                    <  7:146284/149‑1 (MQ=255)

                                                                                                                                                                                     gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGaa                                                                                                                                                                                                                 <  6:667123/149‑1 (MQ=255)

                                                                                                                                                                                     gCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGaa                                                                                                                                                                                                                 <  5:188348/149‑1 (MQ=255)

                                                                                                                                                                                       agagagCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACa                                                                                                                                                                                                               >  6:889229/1‑149 (MQ=255)

                                                                                                                                                                                           agCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCACTCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTCCGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATg                                                                            >  2:95376/1‑275 (MQ=255)

                                                                                                                                                                                              gcCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTc                                                                                                                                                                                                                                            <  6:485474/113‑1 (MQ=255)

                                                                                                                                                                                                  atcgatTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CAcc                                                                                                                                                                                                   >  7:785520/1‑149 (MQ=255)

                                                                                                                                                                                                     gatTGTTGCTACGCTGGTGACAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACcgcg                                                                                                                                                                                                >  7:893056/1‑149 (MQ=255)

                                                                                                                                                                                                      atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt                                                                                                                                                                                                                                              <  5:575458/103‑1 (MQ=255)

                                                                                                                                                                                                      atTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATAtttt                                                                                                                                                                                                                                              >  6:575458/1‑103 (MQ=255)

                                                                                                                                                                                                          ttgCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCt                                                                                                                                                                                           >  8:757503/1‑149 (MQ=255)

                                                                                                                                                                                                              tACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCtttt                                                                                                                                                                                        <  5:840930/148‑1 (MQ=255)

                                                                                                                                                                                                               aCGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTgg                                                                                                                                                                                      <  8:568177/149‑1 (MQ=255)

                                                                                                                                                                                                                   tggtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGt                                                                                                                                                                                  >  6:703502/1‑149 (MQ=255)

                                                                                                                                                                                                                     gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAa‑cac                                                                                                                                                                                                    >  4:218629/1‑129 (MQ=255)

                                                                                                                                                                                                                     gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTg                                                                                                                                                                                >  6:329275/1‑149 (MQ=255)

                                                                                                                                                                                                                     gtggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCctct                                                   >  4:471851/1‑274 (MQ=255)

                                                                                                                                                                                                                       ggCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGc                                                                                                                                                                              >  6:589867/1‑149 (MQ=255)

                                                                                                                                                                                                                            cGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATgg                                                                                                                                                                                                                               >  2:63565/1‑96 (MQ=255)

                                                                                                                                                                                                                            cGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGCGACGCCGGTGGCTTCAATCCCt                                                                                             >  2:607863/1‑225 (MQ=255)

                                                                                                                                                                                                                                gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCa                                                                                                                                                                                                                           <  4:63565/96‑1 (MQ=255)

                                                                                                                                                                                                                                gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt                                                                                                                                                                     >  7:32498/1‑149 (MQ=255)

                                                                                                                                                                                                                                gTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGa                                                                                         <  4:607863/225‑1 (MQ=255)

                                                                                                                                                                                                                                  cAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                                                                                                                                                   <  6:884704/149‑1 (MQ=255)

                                                                                                                                                                                                                                  cAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGATTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTc                                                                                                                                                                   <  5:325472/149‑1 (MQ=255)

                                                                                                                                                                                                                                    aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCAt                                                                                                                                                                 <  5:246623/149‑1 (MQ=255)

                                                                                                                                                                                                                                    aaaGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGAAGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCAt                                                                                                                                                                 >  7:249445/1‑149 (MQ=255)

                                                                                                                                                                                                                                          aTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATAtt                                                                                                                                                                                                           >  8:150036/1‑102 (MQ=255)

                                                                                                                                                                                                                                          aTTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaa                                                                                                                                                           >  7:966174/1‑149 (MQ=255)

                                                                                                                                                                                                                                           ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                                                                                                                                                          <  5:351046/149‑1 (MQ=255)

                                                                                                                                                                                                                                           ttGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTaaaaa                                                                                                                                                          <  7:835492/149‑1 (MQ=255)

                                                                                                                                                                                                                                            tGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACGGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGAt                            <  4:332317/274‑1 (MQ=255)

                                                                                                                                                                                                                                            tGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGAt                            <  2:522792/274‑1 (MQ=255)

                                                                                                                                                                                                                                                  aaaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCg                                                                                                                                                   >  7:496637/1‑149 (MQ=255)

                                                                                                                                                                                                                                                   aagtacacacGCGGGG‑CTATGACCTCGAGTATTGCTTTAATCAACCAGATGATATTTTTCTGAAAGACCATGGGACAGGTGTTTTACCAGATTAAA‑CACCGCGGGGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTCCTCTGCGTGACGCCGGTGGCTTCAAGCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGATGGGTtgtg                    <  4:558700/271‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaaGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                                                                                                                                                <  8:359923/88‑1 (MQ=255)

                                                                                                                                                                                                                                                        cAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATa                                                                                                                                         >  2:298272/1‑153 (MQ=255)

                                                                                                                                                                                                                                                        cAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCtt                                                  >  2:15610/1‑240 (MQ=255)

                                                                                                                                                                                                                                                         aaaCGCGAGGTCTTAG‑CCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTaa                                                                                                                                            >  7:355527/1‑149 (MQ=255)

                                                                                                                                                                                                                                                         aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGttt                                                                                                                                                                                                                    >  3:109192/1‑78 (MQ=255)

                                                                                                                                                                                                                                                         aaaCGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGt                                                                                                                                                                     >  5:431272/1‑124 (MQ=255)

                                                                                                                                                                                                                                                            cgcgAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAg                                                                                                                                     <  4:298272/153‑1 (MQ=255)

                                                                                                                                                                                                                                                             gcgAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAAc                                                                                                                                                                                                                <  1:109192/78‑1 (MQ=255)

                                                                                                                                                                                                                                                               gAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa                                                                                                                                      <  7:365340/149‑1 (MQ=255)

                                                                                                                                                                                                                                                               gAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCa                                                                                                                                      <  5:275155/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                     tAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGCTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACg                                                                                                                                 >  8:731899/1‑149 (MQ=255)

GGATATTGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAATCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTCAATGTACGCTATATGCAGCGTAAACGACTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCCATTGATGTAAAAGACAAACGCGAGG‑CTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTGTTTGAACATATTAAA‑CACCGCGGTGCTTTTGGCTGTTGGCAATCCTTGTTCATCTAAAAAGTAGTCGCCGGTAAATACCAGCACGCCATTACGCTGCGTGACGCCGGTGGCTTCAATCCCTGCGAGCGTATCCTCATGCTCACGAATGATTTTGTTGGCCTCTTTCAACAGCGTTTCGCGGTCGATGGGTTGTGTCTCTTTGTTCATTATTT  >  NC_000913/1330138‑1330673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`