| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,258,252 | T→A | 5.3% | intergenic (‑242/+33) | yhaO ← / ← tdcG | putative transporter/L‑serine dehydratase 3, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,258,252 | 0 | T | A | 5.3% | 324.4 / 7.8 | 94 | intergenic (‑242/+33) | yhaO/tdcG | putative transporter/L‑serine dehydratase 3, anaerobic |
| Reads supporting (aligned to +/- strand): ref base T (43/46); new base A (3/2); total (46/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-01 | |||||||||||
AATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGTGAAACACG > NC_000913/3258104‑3258398 | aaTAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACAtt > 2:564277/1‑149 (MQ=255) aaaGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTtgt < 1:635147/149‑1 (MQ=255) aaaGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGACGGATAAGAATTTTCTACTATATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGCGCACATTtgt < 3:900100/149‑1 (MQ=255) aGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTtgtga > 4:411801/1‑148 (MQ=255) aTGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACAAATGTGCAcct < 1:417502/149‑2 (MQ=255) tACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAgg > 2:787309/1‑149 (MQ=255) aCGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGa < 2:558120/149‑1 (MQ=255) cgcgcgAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGAt < 1:951981/149‑1 (MQ=255) gAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAg > 4:453109/1‑149 (MQ=255) ggTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCt < 1:694220/149‑1 (MQ=255) gTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTg > 3:982421/1‑149 (MQ=255) tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa < 1:314855/149‑1 (MQ=255) tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa > 1:27054/1‑149 (MQ=255) tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa < 3:324511/149‑1 (MQ=255) tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa < 4:581894/149‑1 (MQ=255) tCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa < 2:958892/149‑1 (MQ=255) tCAAAGAAAATATTCACAATTAAGACTTTGAAATAAAGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGa < 4:39821/149‑1 (MQ=255) cAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGAc > 2:6458/1‑149 (MQ=255) gaAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCa > 2:987196/1‑149 (MQ=255) aaaaTCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCaa > 1:526422/1‑149 (MQ=255) tCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTc < 2:907375/149‑1 (MQ=255) cTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCa < 1:136746/149‑1 (MQ=255) tCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGc < 2:373530/149‑1 (MQ=255) cacaATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGcc > 2:585582/1‑149 (MQ=255) tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTtgt < 4:1074140/109‑1 (MQ=255) tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTtgt > 3:1074140/1‑109 (MQ=255) tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGa > 1:595195/1‑149 (MQ=255) tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGa > 3:505536/1‑149 (MQ=255) gACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCAc < 4:560804/149‑1 (MQ=255) aCTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACt < 1:987196/149‑1 (MQ=255) tttGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACttt > 1:926616/1‑149 (MQ=255) tttGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACttt > 1:162816/1‑149 (MQ=255) tGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTaa < 4:1071526/149‑1 (MQ=255) gAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt < 4:491831/149‑1 (MQ=255) gAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt < 2:1056501/149‑1 (MQ=255) aaaTAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt < 1:532930/149‑1 (MQ=255) aaaTAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATg > 4:166241/1‑149 (MQ=255) aaaTAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATg > 2:410711/1‑149 (MQ=255) tAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGcc < 3:735009/149‑1 (MQ=255) gATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCcgcg > 3:540899/1‑149 (MQ=255) tAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTg > 3:379720/1‑149 (MQ=255) aGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGAt < 2:27054/149‑1 (MQ=255) gAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATg > 2:144556/1‑149 (MQ=255) aaTTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGt < 3:15639/149‑1 (MQ=255) tttCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTc < 1:163978/149‑1 (MQ=255) tactaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCgg > 2:799225/1‑149 (MQ=255) tactaAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCgg > 4:1080432/1‑149 (MQ=255) actaAATTAATCGCAGGAGATTATTCATTCTCTATATGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGAAAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGt > 2:464854/1‑149 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCaa > 3:526569/1‑95 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCaa < 4:526569/95‑1 (MQ=255) aaaTTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTAtt > 4:823282/1‑149 (MQ=255) aTTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTa > 2:965394/1‑149 (MQ=255) aTTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTa < 3:339986/149‑1 (MQ=255) tAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATc < 1:965394/149‑1 (MQ=255) cGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTc < 3:1001564/149‑1 (MQ=255) aGGAGAATATTCATTCTCTATTTGCGGCAGGTCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCata > 2:891914/1‑149 (MQ=255) aGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt > 1:172525/1‑106 (MQ=255) aGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAAt < 2:172525/106‑1 (MQ=255) ggAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCatat > 1:1021595/1‑149 (MQ=255) gagaATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATc < 1:144556/149‑1 (MQ=255) aaTATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCttt < 2:162816/149‑1 (MQ=255) ttcattcTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCg > 2:1024665/1‑149 (MQ=255) cattcTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGt < 1:678231/149‑1 (MQ=255) attcTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGtt > 4:388452/1‑149 (MQ=255) ttcTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCCCACAAGGATGAAACCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGttt < 2:969546/149‑1 (MQ=255) ttcTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGttt > 3:70513/1‑149 (MQ=255) ctctATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCa < 1:668257/149‑1 (MQ=255) ctctATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCa < 2:171713/149‑1 (MQ=255) tctATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAgg < 1:920009/50‑1 (MQ=255) tctATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAgg > 2:920009/1‑50 (MQ=255) tctATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCAt > 3:424675/1‑149 (MQ=255) tctATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCAt > 2:1098689/1‑149 (MQ=255) tATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGtt > 3:829965/1‑127 (MQ=255) tATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGtt < 4:829965/127‑1 (MQ=255) tttGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACAt > 2:810757/1‑149 (MQ=255) ttGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGCTGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATc < 2:106578/149‑1 (MQ=255) gATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGAt < 3:126197/149‑1 (MQ=255) gATCAC‑AAAAAAAAGGTGCACAGTTGTGCACCCAAGGATGAAAGCTGACAGAAATGTCATCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCTCGGTATTGATCGTTCATATCTTTGCCGGTTTCATACATCGTCACGAt > 4:674038/1‑149 (MQ=255) aTCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATc > 3:557774/1‑149 (MQ=255) cac‑aAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCAc > 2:483816/1‑149 (MQ=255) c‑aAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACtt < 1:799225/149‑1 (MQ=255) aaaaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACttt > 2:877746/1‑149 (MQ=255) aaaaaaaaGGGGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACttt < 1:825062/149‑1 (MQ=255) aaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCgg < 2:981315/97‑1 (MQ=255) aaaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCgg > 1:981315/1‑97 (MQ=255) aaaaaGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATc < 1:483816/149‑1 (MQ=255) ttgggTGCACAAATGTGCACCTAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGa > 1:984591/4‑149 (MQ=255) ttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGtc > 4:263913/4‑133 (MQ=255) ttgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGtc < 3:263913/130‑1 (MQ=255) tgggTGCACAAATGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAg > 3:1034806/3‑149 (MQ=255) aGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGt > 1:17870/1‑149 (MQ=255) aGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGt < 3:520217/149‑1 (MQ=255) gCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGTGaaa < 4:379720/149‑1 (MQ=255) caTTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGTGAAacac < 3:246877/149‑1 (MQ=255) aTTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGTGAAACAcg > 2:906566/1‑149 (MQ=255) | AATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AAAAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATACATCGTCTCGATCACTTTATCGAGTGAAACACG > NC_000913/3258104‑3258398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |