| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,791,738 | Δ1 bp | 10.2% | coding (466/1449 nt) | gabD → | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,791,738 | 0 | T | . | 10.2% | 196.9 / 16.3 | 59 | coding (466/1449 nt) | gabD | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (26/27); new base . (3/3); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
GAAATCAGCTACGCCGCCTCCTTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGG > NC_000913/2791594‑2791871 | gAAATCAGCTACGCCGCCTCCTTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGaa < 2:83872/149‑1 (MQ=255)gAAATCAGCTACGCCGCCTCCTTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGaa < 2:245651/149‑1 (MQ=255)gAAATCAGCTACGCCGCCTCCTTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGaa < 4:364345/149‑1 (MQ=255) ctcctTTATTGAGTGGGTTGCCGAAGAAGGCAAACGTATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGAATGTGATCAAGCAGCCGATTGGCGTCACCGCGCTTATCATGCCGTGGAACTTCC‑CGGCGGCgatga < 3:590661/149‑1 (MQ=255) tcctTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCgatgat > 3:168418/1‑149 (MQ=255) ctTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTa > 3:221618/1‑149 (MQ=255) ctTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTa > 1:141360/1‑149 (MQ=255) tATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTAccc < 4:221618/149‑1 (MQ=255) ttGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGc > 1:158488/1‑149 (MQ=255) tGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGc < 4:244212/149‑1 (MQ=255) ggTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGcc < 3:337444/149‑1 (MQ=255) agGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGcc > 3:560166/1‑136 (MQ=255) agGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGcc < 4:560166/136‑1 (MQ=255) agGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTgg < 1:127148/149‑1 (MQ=255) ggCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGgc < 3:562459/149‑1 (MQ=255) aaaCGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCTCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGgcagc < 3:22485/149‑1 (MQ=255) aaaCGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCG‑GGAAGTTCCAcggcg < 4:323110/111‑1 (MQ=255) aaaCGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCG‑GGAAGTTCCAcggcg > 3:323110/1‑111 (MQ=255) cGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAgg < 4:168418/149‑1 (MQ=255) gACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGt > 2:13177/1‑149 (MQ=255) gACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCG‑GGAAGTTCCAcggcgtgata > 4:474296/1‑96 (MQ=255) gACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCG‑GGAAGTTCCAcggcgtgata < 3:474296/101‑6 (MQ=255) cATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCga < 1:32758/95‑1 (MQ=255) cATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCga > 2:32758/1‑95 (MQ=255) cATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGa > 1:115746/1‑149 (MQ=255) aTTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGaa < 3:91453/149‑1 (MQ=255) aTTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGaa < 1:13177/149‑1 (MQ=255) tGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCg > 3:76186/1‑149 (MQ=255) tcatcaGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCa > 3:168451/1‑149 (MQ=255) atcaGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGc < 4:229704/135‑1 (MQ=255) atcaGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGc > 3:229704/1‑135 (MQ=255) atcaGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGt > 3:357255/1‑149 (MQ=255) atcaGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGt > 1:19419/1‑149 (MQ=255) tcaGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGACAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTc < 1:361380/149‑1 (MQ=255) aGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGCTTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAg < 4:357255/149‑1 (MQ=255) aGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAg < 2:19419/149‑1 (MQ=255) ggCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGa > 2:7372/1‑149 (MQ=255) gATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCga < 4:13238/76‑1 (MQ=255) gATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCga > 3:13238/1‑76 (MQ=255) gATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCG‑GGAAGTTCCAcggcgt > 4:222272/1‑72 (MQ=37) gATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCG‑GGAAGTTCCAcggcgt < 3:222272/73‑2 (MQ=37) aaaCGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGtt > 2:393597/1‑149 (MQ=255) aCGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTcc > 3:601142/1‑93 (MQ=255) aCGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTcc < 4:601142/93‑1 (MQ=255) gCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTAccc > 2:618564/1‑78 (MQ=255) gCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTAccc < 1:618564/78‑1 (MQ=255) cTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTgc > 4:373264/1‑149 (MQ=255) tGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTgcg > 4:478462/1‑149 (MQ=255) tATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTgcgctggcgc > 1:38075/1‑149 (MQ=255) aTCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTgcgctggcgct > 1:161518/1‑149 (MQ=255) aTCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTgcgctggcgct > 2:30792/1‑149 (MQ=255) tCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTgcgctggcgctg > 3:133071/1‑149 (MQ=255) aaGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGc < 3:485776/66‑1 (MQ=255) aaGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGc > 4:485776/1‑66 (MQ=255) ggCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGAt < 2:38075/149‑1 (MQ=255) gCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGCTGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATc > 2:509398/1‑149 (MQ=255) cgcgGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCgg < 2:140136/149‑1 (MQ=255) cgcgGCTATCACGCCGTGGAACATCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGCTCCGCGCgg < 1:535555/149‑1 (MQ=255) gcgGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCggg < 3:478462/149‑1 (MQ=255) | GAAATCAGCTACGCCGCCTCCTTTATTGAGTGGTTTGCCGAAGAAGGCAAACGCATTTATGGCGACACCATTCCTGGTCATCAGGCCGATAAACGCCTGATTGTTATCAAGCAGCCGATTGGCGTCACCGCGGCTATCACGCCGTGGAACTTCC‑CGGCGGCGATGATTACCCGCAAAGCCGGTCCGGCGCTGGCAGCAGGCTGCACCATGGTGCTGAAGCCCGCCAGTCAGACGCCGTTCTCTGCGCTGGCGCTGGCGGAGCTGGCGATCCGCGCGGG > NC_000913/2791594‑2791871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |