Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,552,833	T→C	10.7%	intergenic (+13/‑68)	uxuA → / → uxuB	mannonate hydrolase/D‑mannonate oxidoreductase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,552,833	0	T	C	10.7%	221.9 / 15.5	75	intergenic (+13/‑68)	uxuA/uxuB	mannonate hydrolase/D‑mannonate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (37/30);  new base C (5/3);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

CCACGGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCA  >  NC_000913/4552688‑4552980                                                                                                                                                  |                                                                                                                                                     ccACGGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGc                                                                                                                                                   >  3:348739/1‑149 (MQ=255)     ggTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcg                                                                                                                                               >  2:274245/1‑149 (MQ=255)      gTCATCAGATGCTGGACTGCCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcgc                                                                                                                                              <  1:620554/149‑1 (MQ=255)        catcaGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTgcgc‑gc                                                                                                                                           <  3:242860/149‑1 (MQ=255)          tcaGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCg                                                                                                                                         >  4:206904/1‑149 (MQ=255)          tcaGATGCTGGACGACCTGAAGAAGAAAACAAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCg                                                                                                                                         >  1:377320/1‑149 (MQ=255)           caGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGt                                                                                                                                        <  3:61487/149‑1 (MQ=255)           caGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGt                                                                                                                                        >  1:408329/1‑149 (MQ=255)                gCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCgg                                                                                                                                   >  2:477014/1‑149 (MQ=255)                gCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGC‑GCGCAGCCATGCCgg                                                                                                                                   <  1:81046/149‑1 (MQ=255)                   ggACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTc                                                                                                                                >  4:448697/1‑149 (MQ=255)                   ggACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTc                                                                                                                                <  3:469461/149‑1 (MQ=255)                       gacCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCcttc                                                                                                                            <  1:178150/149‑1 (MQ=255)                        acCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCcttct                                                                                                                           <  4:348739/149‑1 (MQ=255)                          cTGAAGAAGAAAACCAACCCAGGTTACTCCGCGATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGACTGCGC‑GCCGTGCCGGTTCCttcttc                                                                                                                         >  2:91454/1‑149 (MQ=255)                            gaagaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGTGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCt                                                                                                                       >  4:310059/1‑149 (MQ=255)                            gaagaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGc                                                                                                                                      <  2:334145/134‑1 (MQ=255)                            gaagaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGc                                                                                                                                      >  1:334145/1‑134 (MQ=255)                             aagaagaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCtt                                                                                                                      >  2:188282/1‑149 (MQ=255)                                 agaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCg                                                                                                                  >  3:481653/1‑149 (MQ=255)                                  gaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGt                                                                                                                 >  4:194426/1‑149 (MQ=255)                                  gaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGC‑GCGCAGCCATGCCGGtggat                                                                                                                              <  3:261282/136‑5 (MQ=255)                                  gaaAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCACGGC‑GCGCAGCCATGCCGGtggat                                                                                                                              >  4:261282/1‑132 (MQ=255)                                        caaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCActct                                                                                                           >  3:452070/1‑149 (MQ=255)                                         aaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCtt                                                                                                          <  3:111497/149‑1 (MQ=255)                                             cAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGaa                                                                                                      >  1:85015/1‑149 (MQ=255)                                                gTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGAc                                                                                                   <  3:299733/149‑1 (MQ=255)                                                gTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGAc                                                                                                   <  3:206904/149‑1 (MQ=255)                                                     tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                                              >  2:99990/1‑149 (MQ=255)                                                     tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                                              >  4:423117/1‑149 (MQ=255)                                                      ccGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTTGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTc                                                                                             >  4:99045/1‑149 (MQ=255)                                                       cGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGAc                                                                                                   <  2:282271/142‑1 (MQ=255)                                                       cGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGAc                                                                                                   >  1:282271/1‑142 (MQ=255)                                                       cGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCt                                                                                            >  3:460603/1‑149 (MQ=255)                                                        gCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGTGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTg                                                                                           <  3:310059/149‑1 (MQ=255)                                                        gCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTg                                                                                           <  1:188282/149‑1 (MQ=255)                                                          aaTTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGa                                                                                         <  2:408329/149‑1 (MQ=255)                                                          aaTTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGa                                                                                         <  3:448697/149‑1 (MQ=255)                                                               gtcgtcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTa                                                                                    >  4:562423/1‑149 (MQ=255)                                                                tcgtcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTAc                                                                                   >  4:472557/1‑149 (MQ=255)                                                                     tGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGa                                                                              <  4:452070/149‑1 (MQ=255)                                                                      gAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGac                                                                             >  2:161244/1‑149 (MQ=255)                                                                      gAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGac                                                                             <  4:481653/149‑1 (MQ=255)                                                                       aaaGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGact                                                                            >  1:169108/1‑149 (MQ=255)                                                                        aaGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGacta                                                                           <  1:99990/149‑1 (MQ=255)                                                                         aGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGactac                                                                          >  3:207722/1‑149 (MQ=255)                                                                         aGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGactac                                                                          <  3:562423/149‑1 (MQ=255)                                                                               ggCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAttgt                                                                    >  1:437041/1‑149 (MQ=255)                                                                                  cGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGa                                                                 <  2:85015/149‑1 (MQ=255)                                                                                  cGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGa                                                                 <  1:477014/149‑1 (MQ=255)                                                                                   gAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGAc                                                                <  1:611352/149‑1 (MQ=255)                                                                                     aGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAg                                                              >  1:170957/1‑149 (MQ=255)                                                                                      gTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGc                                                             >  1:86227/1‑149 (MQ=255)                                                                                       ttCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGa                                                                              <  3:441974/131‑1 (MQ=255)                                                                                       ttCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGa                                                                              >  4:441974/1‑131 (MQ=255)                                                                                              tgtCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGcc                                                     <  3:335509/149‑1 (MQ=255)                                                                                                    aCTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATGTCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATACTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgc                                               <  3:423117/149‑1 (MQ=255)                                                                                                          gATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgccc                                         <  3:194426/149‑1 (MQ=255)                                                                                                           ctCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATAGTTGACAGCAATCTGCCGGTTGcccgcccg                                        <  2:377320/148‑1 (MQ=255)                                                                                                                gcgcgcTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTg                                                                                           >  2:235088/1‑93 (MQ=255)                                                                                                                gcgcgcTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTg                                                                                           <  1:235088/93‑1 (MQ=255)                                                                                                                      tttctttAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCa                             >  4:586208/1‑149 (MQ=255)                                                                                                                      tttctttAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCa                             >  4:269229/1‑149 (MQ=255)                                                                                                                                       atCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCAc            >  1:476365/1‑149 (MQ=255)                                                                                                                                       atCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCAc            <  3:269229/149‑1 (MQ=255)                                                                                                                                       atCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCAc            >  2:429399/1‑149 (MQ=255)                                                                                                                                        tCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACg           >  4:295157/1‑149 (MQ=255)                                                                                                                                         gaaCCGGCACGGC‑GCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGc          >  3:253361/3‑149 (MQ=255)                                                                                                                                           aCCGGCACGGC‑GCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAt        >  1:358639/1‑149 (MQ=255)                                                                                                                                            ccGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAtt       <  1:292199/149‑1 (MQ=255)                                                                                                                                            ccGGCACGGC‑GCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGa                                                                                         >  2:136503/1‑67 (MQ=25)                                                                                                                                            ccGGCACGGC‑GCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGa                                                                                         <  1:136503/67‑1 (MQ=25)                                                                                                                                            ccGGCACGGC‑GCGCAGCCATGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCAtt       >  2:13754/1‑149 (MQ=255)                                                                                                                                               gCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATtgtg    >  2:161349/1‑149 (MQ=255)                                                                                                                                                 aTGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCa  >  2:127173/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     CCACGGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGC‑GCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCA  >  NC_000913/4552688‑4552980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑