Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	756,643	T→A	11.1%	I246N (ATT→AAT)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,643	0	T	A	11.1%	202.9 / 15.4	63	I246N (ATT→AAT)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base A (2/5);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

CTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATC  >  NC_000913/756496‑756791                                                                                                                                                     |                                                                                                                                                     cTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCCGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCAtt                                                                                                                                                     <  4:367543/149‑1 (MQ=255)  tACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTg                                                                                                                                                    >  4:137350/1‑149 (MQ=255)    ccGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCAATGcc                                                                                                                                                  <  2:386694/149‑1 (MQ=255)       tGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGt                                                                                                                                               >  1:107019/1‑149 (MQ=255)          tGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCg                                                                                                                                            >  1:434696/1‑149 (MQ=255)           ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGCACCGGCAATGCCGGtcgg                                                                                                                                            <  1:403883/149‑4 (MQ=255)           ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCgg                                                                                                                                           >  2:41322/1‑149 (MQ=255)           ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGCTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGCACCGGCAATGCCGGtcgg                                                                                                                                            <  4:350932/149‑4 (MQ=255)                     ggAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGt                                                                                                                                 >  2:232657/1‑149 (MQ=255)                           gggCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCAATGCCGGtcgggt                                                                                                                                          <  3:18560/134‑6 (MQ=255)                           gggCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCAATGCCGGtcgggt                                                                                                                                          >  4:18560/1‑129 (MQ=255)                             gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAg                                                                                                                         <  4:160289/149‑1 (MQ=255)                             gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCAATGCCGGtcgggt                                                                                                                                          >  2:80246/1‑127 (MQ=255)                             gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCAATGCCGGtcgggt                                                                                                                                          <  1:80246/132‑6 (MQ=255)                               gTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGt                                                                                                                       <  1:41322/149‑1 (MQ=255)                                tATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                                      >  1:596911/1‑149 (MQ=255)                                tATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                                      <  2:603893/149‑1 (MQ=255)                                  tttATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGc                                                                                                                    <  2:423994/149‑1 (MQ=255)                                   ttATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGcc                                                                                                                   <  1:201903/149‑1 (MQ=255)                                         gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTAc                                                                                                                                     <  1:351863/125‑1 (MQ=255)                                         gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTAc                                                                                                                                     >  2:351863/1‑125 (MQ=255)                                          tCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGa                                                                                                            <  3:53958/149‑1 (MQ=255)                                           ccaccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGaa                                                                                                           <  3:137350/149‑1 (MQ=255)                                               caccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCg                                                                                                       >  1:138283/1‑149 (MQ=255)                                                    aCGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGc                                                                                                        >  4:230883/1‑143 (MQ=255)                                                    aCGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGc                                                                                                        <  3:230883/143‑1 (MQ=255)                                                        ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                                              <  3:591673/149‑1 (MQ=255)                                                        ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                                              <  3:391346/149‑1 (MQ=255)                                                             ttAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaac                                                                                         >  2:594738/1‑149 (MQ=255)                                                               aaCACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaa                                                                                       >  3:220933/1‑149 (MQ=255)                                                                acacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaa                                                                                      <  1:54070/149‑1 (MQ=255)                                                                acacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCAGCATTGCCGGGGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaa                                                                                      <  2:509337/149‑1 (MQ=255)                                                                 cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                                                     >  3:57352/1‑149 (MQ=255)                                                                     ggCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATgg                                                                                 <  3:15460/149‑1 (MQ=255)                                                                      gCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGc                                                                                >  1:457160/1‑149 (MQ=255)                                                                        gACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGa                                                                              >  3:602985/1‑149 (MQ=255)                                                                             tgtCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtt                                                                         <  2:138283/149‑1 (MQ=255)                                                                                 ggCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTAt                                                                     >  1:623594/1‑149 (MQ=255)                                                                                      ggCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtg                                                                                                                >  1:554635/1‑101 (MQ=255)                                                                                      ggCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtg                                                                                                                <  2:554635/101‑1 (MQ=255)                                                                                      ggCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                                                     >  3:251603/1‑128 (MQ=255)                                                                                      ggCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                                                     <  4:251603/128‑1 (MQ=255)                                                                                      ggCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGc                                                                <  2:457160/149‑1 (MQ=255)                                                                                       gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaa                                                                                      <  3:185407/126‑1 (MQ=255)                                                                                       gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaa                                                                                      >  4:185407/1‑126 (MQ=255)                                                                                        cTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGt                                                              <  1:594738/149‑1 (MQ=255)                                                                                         tATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtttt                                                                       <  2:267820/139‑1 (MQ=255)                                                                                         tATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtttt                                                                       >  1:267820/1‑139 (MQ=255)                                                                                           tCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTAt                                                           >  3:310168/1‑149 (MQ=255)                                                                                              gTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgcg                                                        >  1:476283/1‑149 (MQ=255)                                                                                              gTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgcg                                                        >  3:155494/1‑149 (MQ=255)                                                                                                gCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGcc                                                      <  2:596911/149‑1 (MQ=255)                                                                                                   ggCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGaa                                                   >  3:16005/1‑149 (MQ=255)                                                                                                    gCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAAc                                                  <  3:285168/149‑1 (MQ=255)                                                                                                                aGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTg                                      <  1:69778/149‑1 (MQ=255)                                                                                                                        gAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCg                              >  2:88590/1‑149 (MQ=255)                                                                                                                              tgGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGt                        >  4:348962/1‑149 (MQ=255)                                                                                                                               ggCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTg                       >  4:98617/1‑149 (MQ=255)                                                                                                                                    ttCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTgc                  >  2:449200/1‑149 (MQ=255)                                                                                                                                          ccG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTc            >  3:389378/1‑149 (MQ=255)                                                                                                                                           cG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTcc           <  3:258409/149‑1 (MQ=255)                                                                                                                                              aCCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCcat         <  2:107019/149‑1 (MQ=255)                                                                                                                                                cGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCcatca       <  4:16005/149‑1 (MQ=255)                                                                                                                                                 ggCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCcatcat      >  1:609385/1‑149 (MQ=255)                                                                                                                                                     ttGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATc  <  4:220933/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCG‑ACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATC  >  NC_000913/756496‑756791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑