| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,053,566:1 | +A | 10.0% | intergenic (+148/+81) | hemN → / ← yshB | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,053,566 | 1 | . | A | 10.0% | 160.1 / 14.0 | 40 | intergenic (+148/+81) | hemN/yshB | coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base . (22/14); new base A (2/2); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
TGGCGCTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGGTGTGTGGCTG > NC_000913/4053427‑4053713 | tGGCGCTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑t > 1:1021523/1‑141 (MQ=255)tGGCGCTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑t < 2:1021523/141‑1 (MQ=255) gcTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGcc < 1:932353/149‑1 (MQ=255) cTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatg < 4:987190/143‑1 (MQ=255) cTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatg > 3:987190/1‑143 (MQ=255) aaaGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatgatgtc < 2:1490871/138‑3 (MQ=255) aaaGTAGAAAACGAAAGCGCCATCAATGTCCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGcc > 1:1490871/1‑138 (MQ=255) gAAAGCGCCATCAATGTGCTGAGCACAAGATTGACTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATCATGCTCA‑GATGCCTGATgcg < 4:1183486/133‑1 (MQ=37) gAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAGATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATCATGCTCA‑GATGCCTGATgcg > 3:1183486/1‑133 (MQ=38) aCAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGc > 4:1403010/1‑101 (MQ=255) aCAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGc < 3:1403010/101‑1 (MQ=255) cAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATCATGCTCA‑GATGCCTGAt < 2:90712/105‑1 (MQ=37) cAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTGGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATCATGCTCA‑GATGCCTGAt > 1:90712/1‑105 (MQ=25) gATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatgatg > 1:1067624/1‑96 (MQ=255) gATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatgatg < 2:1067624/96‑1 (MQ=255) tGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatgat < 2:716130/85‑1 (MQ=255) tGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCatgat > 1:716130/1‑85 (MQ=255) ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGCCt > 3:1325439/1‑62 (MQ=255) ttattGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGCCt < 4:1325439/62‑1 (MQ=255) ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGcc > 4:632184/1‑54 (MQ=255) ttttGTAGGCAGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGcc < 3:632184/54‑1 (MQ=255) aTCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCaa < 3:746573/107‑1 (MQ=255) aTCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCaa > 4:746573/1‑107 (MQ=255) aTCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtg > 1:1406359/1‑149 (MQ=255) aTCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtg > 1:166960/1‑149 (MQ=255) tcgggCATC‑TGAGCATGATGCTTGATGCGACGCTGGTCGCGTCTTATCAGGCCTAGGACTTTCTCCGCATCCGGCATAAGCAACGTGCTGCAGCTAAACAGCCCAGTCATTGTGGCACAGAGAGCGGCAGCAACGGCAGTTTGCGgtgt > 4:1176546/4‑149 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGc > 4:1450511/1‑94 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGc < 3:1450511/94‑1 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtgt > 2:1183216/1‑149 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtgt > 4:1118636/1‑149 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtgt < 1:1079171/149‑1 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtgt > 3:1587239/1‑149 (MQ=255) tCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtgt > 2:1079171/1‑149 (MQ=255) aGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt > 3:1730335/1‑88 (MQ=255) aGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCtt < 4:1730335/88‑1 (MQ=255) aGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGgtgtgt > 4:195932/1‑149 (MQ=255) ggCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGACAGCAACGGCAGTTTGCGGtgtgtg > 4:1814685/1‑149 (MQ=255) gCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGccc < 3:719158/100‑1 (MQ=255) gCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGccc > 4:719158/1‑100 (MQ=255) aTC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCa > 2:352206/1‑118 (MQ=255) aTC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCa < 1:352206/118‑1 (MQ=255) tC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGGTGTGTGGCTg > 4:1036462/1‑149 (MQ=255) | TGGCGCTTCGTTTACAAAGTAGAAAACGAAAGCGCCATCAATGTGCTGAGAACAAGATTGCCTGATGCGCTGGGCAACAAAACCTGTTGAAATTATTGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATC‑TGAGCATGATGCCTGATGCGGCGCTGGTCGCGTCTTATCAGGCCTACGACTTTCTCCGCATCCGGCATAAACAACGAGCTTCAGCTAAACAGCCCAATCATTGCGGCACACAGCACGGCAGCAACGGCAGTTTGCGGTGTGTGGCTG > NC_000913/4053427‑4053713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |