| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,706,121:1 | +T | 10.1% | coding (1004/1800 nt) | lepA ← | back‑translocating elongation factor EF4, GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,706,121 | 1 | . | T | 10.1% | 579.0 / 66.9 | 149 | coding (1004/1800 nt) | lepA | back‑translocating elongation factor EF4, GTPase |
| Reads supporting (aligned to +/- strand): ref base . (65/69); new base T (4/11); total (69/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01 | |||||||||||
Only 100 of 153 total aligned reads displayed.
ACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAG > NC_000913/2705974‑2706268 | aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑c < 4:724233/149‑1 (MQ=255)aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑c > 2:873345/1‑149 (MQ=255)aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑c > 3:1869384/1‑149 (MQ=255)aCGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACAGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGc‑c > 2:821395/1‑149 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTgg > 2:572342/1‑149 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTgg > 3:685752/1‑149 (MQ=255) ttCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATAAATTCCAGATCGTATTAACTTTCCAGCCTTCCTTGGTTGACCTCATTTGGCGGCAGCCCGAGGAACCCGCAGCGAAAACCAAAGC‑CAAgcgcccggg > 1:744309/1‑144 (MQ=255) tctcTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGa > 2:727163/1‑149 (MQ=255) tctTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTgggc > 1:127539/1‑147 (MQ=255) ctTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCt < 4:647548/149‑1 (MQ=255) ttGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCtt > 4:1488805/1‑149 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCttt < 2:127539/149‑1 (MQ=255) tGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCttt < 1:572342/149‑1 (MQ=255) gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 2:977124/149‑2 (MQ=255) gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 3:1125509/149‑2 (MQ=255) gACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 2:1279564/149‑2 (MQ=255) aCGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttgg < 1:1620603/149‑3 (MQ=255) aCGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTcc < 4:1764550/149‑1 (MQ=255) cGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttggt < 2:1099725/149‑4 (MQ=255) gTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCgg > 2:1769741/1‑149 (MQ=255) ggTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCt < 4:1121426/149‑1 (MQ=255) tttCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCa < 4:439621/149‑1 (MQ=255) aaCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCt < 1:131574/129‑1 (MQ=255) ttCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 1:335959/1‑149 (MQ=255) cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGt < 1:1769741/149‑1 (MQ=255) aTACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCttt < 2:1353875/131‑1 (MQ=255) aTACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCttt > 1:1353875/1‑131 (MQ=255) tACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGa < 3:493765/149‑1 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg < 3:444090/149‑1 (MQ=255) acacTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg > 1:1184701/1‑149 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACGAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa < 4:1565607/142‑1 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg > 3:124715/1‑127 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttg < 4:124715/128‑2 (MQ=255) acTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 3:1565607/1‑142 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttggtttc < 3:1820597/124‑7 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCTCCAGCGCGCTGG‑GCtttggtttc > 4:1820597/1‑118 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAg < 4:1869384/149‑1 (MQ=255) ggCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCAGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAg > 4:703220/1‑149 (MQ=255) gcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCGTAGAACAGTGAGGCATCGTTCAGGCt > 4:96351/1‑149 (MQ=255) cAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTg > 1:558686/1‑149 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCGGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa < 3:1550978/149‑1 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCGTAGAACAGTGAGGCATCGTTCAGGCTGa < 3:96351/149‑1 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAgg > 2:1737894/1‑145 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAgg < 1:1737894/145‑1 (MQ=255) aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa < 3:1488805/149‑1 (MQ=255) tCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGaa < 1:112990/118‑1 (MQ=255) tCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGaa > 2:112990/1‑118 (MQ=255) agatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAg > 1:1235211/1‑149 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTc > 4:1269708/1‑131 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTc < 3:1269708/131‑1 (MQ=255) gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgc > 3:353639/1‑149 (MQ=255) atcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGAAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCAc > 1:104642/1‑149 (MQ=255) tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa < 3:1680935/149‑1 (MQ=255) ttCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgc < 2:570883/136‑1 (MQ=255) ttCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaatg > 1:928794/1‑147 (MQ=255) ttCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAgg < 2:1184701/149‑1 (MQ=255) cACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCt < 3:703220/149‑1 (MQ=255) aCGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACa > 1:1543187/1‑101 (MQ=255) cGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTc < 1:873345/149‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa < 1:1835751/119‑1 (MQ=255) ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGa > 2:1835751/1‑119 (MQ=255) aCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAgtcgt > 3:1016285/1‑149 (MQ=255) aCGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAgtcgt > 1:814275/1‑149 (MQ=255) ttCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCgg < 4:1367248/149‑1 (MQ=255) ccTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGaa < 2:335959/149‑1 (MQ=255) tGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACt > 3:1770929/1‑149 (MQ=255) gatgatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATa < 1:624835/78‑1 (MQ=255) gatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCg < 4:353639/149‑1 (MQ=255) gatCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCg > 4:1678240/1‑149 (MQ=255) tctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg > 4:882587/1‑83 (MQ=255) tctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAg < 3:882587/83‑1 (MQ=255) tctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCCTCATAGTCGTCGGAACTTACCggg > 3:99353/1‑149 (MQ=255) ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTTAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTc < 1:164367/126‑1 (MQ=255) ctcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTTAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTc > 2:164367/1‑126 (MQ=255) tcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa < 3:1555259/91‑1 (MQ=255) tcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCa > 4:1555259/1‑91 (MQ=255) tcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGaa < 2:744309/149‑1 (MQ=255) tcCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGaa > 3:311754/1‑149 (MQ=255) tgtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTcc < 2:320761/59‑1 (MQ=255) gtgCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc > 1:1524169/1‑149 (MQ=255) agcagGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGc > 1:31764/1‑149 (MQ=255) gcagGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCg > 2:1609313/1‑149 (MQ=255) ccGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATAc > 4:443382/1‑149 (MQ=255) aGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTg > 4:923358/1‑149 (MQ=255) ggAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGc < 3:33051/149‑1 (MQ=255) gAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCg < 3:923358/149‑1 (MQ=255) ccGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGttt < 2:1235211/149‑1 (MQ=255) cGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTg < 4:340441/142‑1 (MQ=255) gCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGa < 2:1524169/149‑1 (MQ=255) gCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGa > 3:389257/1‑149 (MQ=255) ggAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTACATAGTCGTCGGAACTTACCGGGAACAGACCGGCGta < 1:1684242/130‑1 (MQ=255) ggAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTACATAGTCGTCGGAACTTACCGGGAACAGACCGGCGta > 2:1684242/1‑130 (MQ=255) gAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCt > 4:1129088/1‑149 (MQ=255) aCCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTaa < 4:1770929/149‑1 (MQ=255) aaaGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCAGCGGTTTGACTTTCTTAAAGc > 3:593182/1‑149 (MQ=255) aGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa < 4:311754/149‑1 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa < 2:1343374/148‑1 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCa > 1:1343374/1‑148 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAg < 4:593182/149‑1 (MQ=255) gC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAg > 4:1635267/1‑149 (MQ=255) | ACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGC‑CCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGCCAG > NC_000913/2705974‑2706268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |