Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,620,216	C→T	21.0%	intergenic (‑56/+30)	uraA ← / ← upp	uracil permease/uracil phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,620,216	0	C	T	21.0%	36.1 / 6.4	19	intergenic (‑56/+30)	uraA/upp	uracil permease/uracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base T (2/2);  total (9/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

CAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCG  >  NC_000913/2620073‑2620345                                                                                                                                                |                                                                                                                                  cAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTaaa                                                                                                                              <  1:824487/149‑1 (MQ=255)                aaTGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTaaa                                                                                                                              >  1:1618985/1‑134 (MQ=255)                aaTGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTaaa                                                                                                                              <  2:1618985/134‑1 (MQ=255)                                  ggATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTaaa                                                                                                                              >  3:1569494/1‑116 (MQ=255)                                  ggATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTaaa                                                                                                                              <  4:1569494/116‑1 (MQ=255)                                                          tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGc                                                                                                                        >  4:676400/1‑98 (MQ=255)                                                          tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGc                                                                                                                        <  3:676400/98‑1 (MQ=255)                                                                          aGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCg                                                                                                                          >  1:1858354/1‑80 (MQ=255)                                                                          aGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCg                                                                                                                          <  2:1858354/80‑1 (MQ=255)                                                                                                            tgtgTTATAGGGGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTc                                                                                                                           >  1:1394302/1‑45 (MQ=255)                                                                                                            tgtgTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTc                                                                                                                           <  2:1394302/45‑1 (MQ=255)                                                                                                                    aGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTAttt                                                                                                             <  1:394157/51‑1 (MQ=255)                                                                                                                    aGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTAttt                                                                                                             >  2:394157/1‑51 (MQ=255)                                                                                                                      gcgcTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTc                                                                                               >  4:926420/1‑63 (MQ=255)                                                                                                                      gcgcTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTc                                                                                               <  3:926420/63‑1 (MQ=255)                                                                                                                                    ttaaaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGtt    <  4:1435127/138‑1 (MQ=255)                                                                                                                                    ttaaaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGtt    >  3:1435127/3‑140 (MQ=255)                                                                                                                                            gACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCg  <  4:1284494/134‑1 (MQ=255)                                                                                                                                            gACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCg  >  3:1284494/1‑134 (MQ=255)                                                                                                                                                |                                                                                                                                  CAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCG  >  NC_000913/2620073‑2620345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑