BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,339,828:1	+T	5.4%	coding (238/810 nt)	mlaF ←	ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,828	1	.	T	5.4%	463.4 / 21.2	111	coding (238/810 nt)	mlaF	ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand): ref base . (59/46); new base T (2/4); total (61/50)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

Only 100 of 114 total aligned reads displayed.

CCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAT  >  NC_000913/3339576‑3340090

ccGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGCAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGa                                                                                                                                                                                                                                                  >  1:114446/1‑275 (MQ=255)

          cgcAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCCCa                                                                                                                                                                                                                                         >  2:166780/1‑274 (MQ=255)

                         cAGCGCTGCCAGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtct                                                                                                                                                                                                                         <  2:460119/275‑1 (MQ=255)

                             gcTGCACGCCGCGCCATCCAACCGGAAAGTTCCAAAGGCATCAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTATGATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAcc                                                                                                                                                                                                                     <  4:300887/275‑1 (MQ=255)

                                                            cAGAAGGCATTATTTTAGCCGCACCCAGNGNGCCCACGGCCTCAAGCTTCATCATCCCCGTACTATGCAACAAGGGNGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACNCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGa                                                                                                                                                                                      <  3:42799/275‑1 (MQ=255)

                                                                               cgacgcACGGAGCCCCACGCACCCTAGCTNNAACATCAACCGACGATGCAACAATGGCGCGNAGAGTTGGGTATGTTAGCGCAGTGGATAGGCGACGTTGTAAAATACGTTCATATCATTGAACAACGACCCGGACNGAAATAACCTGCTCATCCGGTTGCGCACTGTAGACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAc                                                                                                                                                                   <  3:60647/270‑1 (MQ=255)

                                                                                 cTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGCTCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAcc                                                                                                                                                                  >  2:12481/1‑274 (MQ=255)

                                                                                  tCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATCCCG‑GCGCGAACGAGACATCCCCGCAATATTCCCACCATCGAACAGGATCCCACCATGAACTGGTGCGCTTTGCCCGCCAATCAAGCGGACTCGCGTCGTTTTACCGa                                                                                                                                                                >  4:129949/1‑275 (MQ=255)

                                                                                                         gCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc                                                                                                                                                                                             <  2:346721/223‑1 (MQ=255)

                                                                                                           ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gcg                                                                                                                                                                                                                                                                     >  5:232479/1‑149 (MQ=255)

                                                                                                           ttCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gcg                                                                                                                                                                                                                                                                     >  7:416028/1‑149 (MQ=255)

                                                                                                               tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGaa                                                                                                                                                                                                                                                                 >  7:286783/1‑149 (MQ=255)

                                                                                                                catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAAc                                                                                                                                                                                                                                                                <  8:125995/149‑1 (MQ=255)

                                                                                                                 atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg                                                                                                                                                                                                                                                               >  8:212454/1‑149 (MQ=255)

                                                                                                                 atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACg                                                                                                                                                                                                                                                               >  8:332774/1‑149 (MQ=255)

                                                                                                                  tcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACgg                                                                                                                                                                                                                                                              <  5:34897/149‑2 (MQ=255)

                                                                                                                    aCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACgaga                                                                                                                                                                                                                                                            <  6:289526/149‑1 (MQ=255)

                                                                                                                     ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt                                                                                                                             <  2:129950/275‑1 (MQ=255)

                                                                                                                     ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACggat                                                                                                                                                                                                                                                            >  6:72679/1‑145 (MQ=255)

                                                                                                                     ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACggat                                                                                                                                                                                                                                                            <  5:72679/148‑4 (MQ=255)

                                                                                                                             tGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACGCCGTGACCTTCCCTCGCGGCAc                                                                                                                     >  2:510805/1‑275 (MQ=255)

                                                                                                                                aacaacGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAGAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGt                                                                                                                  <  2:100247/275‑1 (MQ=255)

                                                                                                                                aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat                                                                                                                                                                                                                                                >  5:135469/1‑149 (MQ=255)

                                                                                                                                aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat                                                                                                                                                                                                                                                >  6:186641/1‑149 (MQ=255)

                                                                                                                                aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAat                                                                                                                                                                                                                                                >  8:50186/1‑149 (MQ=255)

                                                                                                                                aacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCCCgg                                                                                                                   >  2:446910/1‑274 (MQ=255)

                                                                                                                                  caaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAACCCCGTAATCTTCCCTcg                                                                                                                           >  1:44597/1‑264 (MQ=255)

                                                                                                                                      ggCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtctc                                                                                                                                                                                                                                          <  8:416028/149‑1 (MQ=255)

                                                                                                                                                     gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATGACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAt                                                                                                                                                                                                                           >  8:60195/1‑149 (MQ=255)

                                                                                                                                                         gTTCGCGCAGTGGATAGTCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCNGAATATTNCCANCATCGANAAGGAt                                                                                                                                                                                                                           >  3:24497/1‑145 (MQ=255)

                                                                                                                                                             gcgcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCa                                                                                                                                                                                                                       <  1:24497/145‑1 (MQ=255)

                                                                                                                                                                 aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATc                                                                                                                                                                                                               <  7:332774/149‑1 (MQ=255)

                                                                                                                                                                  gTGGATAGGCGACTTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAAGAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGcc                                                                                 <  2:395219/274‑1 (MQ=255)

                                                                                                                                                                    ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTgg                                                                                                                                                                                                            >  5:348281/1‑149 (MQ=255)

                                                                                                                                                                    ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGcc                                                                                                                                                            >  4:162151/1‑197 (MQ=255)

                                                                                                                                                                      aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTg                                                                                                                                                                                                          <  5:186641/149‑1 (MQ=255)

                                                                                                                                                                        aGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGAt                                                                                                                                                        <  2:162153/197‑1 (MQ=255)

                                                                                                                                                                          gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                                                                                                                                                      >  7:33318/1‑149 (MQ=255)

                                                                                                                                                                          gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                                                                                                                                                      <  6:56557/149‑1 (MQ=255)

                                                                                                                                                                           cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑gcg                                                                                                                                                                                                                                                                     >  3:76239/1‑85 (MQ=255)

                                                                                                                                                                           cGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt                                                                                                                                                                                                     <  7:50186/149‑1 (MQ=255)

                                                                                                                                                                            gACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            >  5:30196/1‑109 (MQ=255)

                                                                                                                                                                               gTTGTCAAATACGTTCATATCATTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACg                                                                                                                                                                                                                                                               <  7:143844/87‑1 (MQ=25)

                                                                                                                                                                               gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGaa                                                                                                                                                                                                                                                                 <  1:76239/85‑1 (MQ=255)

                                                                                                                                                                               gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGC‑CTGTATACAGTGCGCAAACg                                                                                                                                                                                                                                                               >  8:143844/1‑87 (MQ=37)

                                                                                                                                                                                 tGTCAAATACGTTCATATCAGTGAACAACGCCCAGGACTGAAAGAACATGCGCATCCTTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt                                                                                                                             <  2:322815/215‑1 (MQ=255)

                                                                                                                                                                                  gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc                                                                                                                                                                                              <  7:212454/149‑1 (MQ=255)

                                                                                                                                                                                   tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATCACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCCGCACGGTCAGGGAACTATTATCGACGATGCCGCGATTGCCACGTGTaaaaattaaatc                                                               >  1:39390/1‑267 (MQ=255)

                                                                                                                                                                                   tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga                                                                                                                                                                                                                                                              <  8:80836/84‑1 (MQ=255)

                                                                                                                                                                                   tCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACga                                                                                                                                                                                                                                                              >  7:80836/1‑84 (MQ=255)

                                                                                                                                                                                     aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt                                                                                                                                                                                                           >  6:402135/1‑133 (MQ=255)

                                                                                                                                                                                     aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGt                                                                                                                                                                                                           <  5:402135/133‑1 (MQ=255)

                                                                                                                                                                                     aaaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCaa                                                                                                                                                                                           >  5:456740/1‑149 (MQ=255)

                                                                                                                                                                                       aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATc                                                                                                                                                                                         >  5:158925/1‑149 (MQ=255)

                                                                                                                                                                                        tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg                                                                                                                                                                                                                              <  8:379309/111‑1 (MQ=255)

                                                                                                                                                                                        tACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg                                                                                                                                                                                                                              >  7:379309/1‑111 (MQ=255)

                                                                                                                                                                                           gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCCCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCCGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACCTcgccgata                                                        >  2:194509/1‑269 (MQ=255)

                                                                                                                                                                                            ttCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACg                                                                                                                                                                                    >  8:300465/1‑149 (MQ=255)

                                                                                                                                                                                             tCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACTCGTAAAACTGACATCGCGCATATCg                                                     >  2:273296/1‑275 (MQ=255)

                                                                                                                                                                                                tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGt                                                                                                                                                                                >  5:384742/1‑149 (MQ=255)

                                                                                                                                                                                                 atCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTa                                                                                                                                                                               >  8:108497/1‑149 (MQ=255)

                                                                                                                                                                                                  tCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAg                                                                                                                                                                              >  8:267472/1‑149 (MQ=255)

                                                                                                                                                                                                    cgataacaacGCCCCAGACTGAAATAACATGCTCATCCGTTTGCGCACGGTATACAG‑GCGCTAACGGGACATCGCCGGAATATTCTCACCATCGAAAAGGTGCTCACCAGGATCTGGTGCGATTGGCCCGCCAAGCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAAt                                              <  4:273292/271‑1 (MQ=255)

                                                                                                                                                                                                    aGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcg                                                                                                                                                                            >  6:429885/1‑149 (MQ=255)

                                                                                                                                                                                                     gTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgt                                                                                                                                                                           >  8:273549/1‑149 (MQ=255)

                                                                                                                                                                                                      tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtc                                                                                                                                                                          >  7:17042/1‑149 (MQ=255)

                                                                                                                                                                                                       gAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcg                                                                                                                                                                         <  6:232479/149‑1 (MQ=255)

                                                                                                                                                                                                        aacaacGCCCCGGACCGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt                                                                                                                                                                        >  8:125793/1‑149 (MQ=255)

                                                                                                                                                                                                           aacGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtct                                                                                                                                                                                                                         >  3:14813/1‑97 (MQ=255)

                                                                                                                                                                                                            aagCCCCGGACTGAAAAAACCATTGCATCCGTTTGCGAAAGGGAAACAG‑GCGCGAACGAGACAGCGCCGGAATATTCTCACCATCGAAAAGGGTCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTGACCGATGCCCGATGGACCCATGATCGCCGTGATCTTCCCTCGCGGCACTGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCcaca                                      <  4:194507/273‑1 (MQ=255)

                                                                                                                                                                                                             cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCCAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATAACGACTAAATTCGccccc                                      >  2:9835/1‑271 (MQ=255)

                                                                                                                                                                                                               ccccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCAcc                                                                                                                                                                                                                     <  1:14813/97‑1 (MQ=255)

                                                                                                                                                                                                               ccccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAt                                                                                                                                             <  3:83597/169‑1 (MQ=255)

                                                                                                                                                                                                                cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                                                                                                                                               >  5:380537/1‑86 (MQ=255)

                                                                                                                                                                                                                cccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                                                                                                                                               <  6:380537/86‑1 (MQ=255)

                                                                                                                                                                                                                 ccGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGAt                                                                                                                                                               <  7:108497/149‑1 (MQ=255)

                                                                                                                                                                                                                  cGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                                                                                                                                               >  1:78891/1‑84 (MQ=255)

                                                                                                                                                                                                                       tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc                                                                                                                                                             >  8:229791/1‑145 (MQ=255)

                                                                                                                                                                                                                       tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGc                                                                                                                                                             >  6:453857/1‑145 (MQ=255)

                                                                                                                                                                                                                       tGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGa                                                                                                                                                         >  8:430013/1‑149 (MQ=255)

                                                                                                                                                                                                                         aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            <  6:51822/64‑1 (MQ=255)

                                                                                                                                                                                                                         aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATtc                                                                                                                                                                                                                                            >  5:51822/1‑64 (MQ=255)

                                                                                                                                                                                                                         aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa                                                                                                                                                                                  >  6:281077/1‑122 (MQ=255)

                                                                                                                                                                                                                         aaaTAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGa                                                                                                                                                                                  <  5:281077/122‑1 (MQ=255)

                                                                                                                                                                                                                           aTAACATGCTCCTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCTGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAAtt                       <  1:56522/275‑1 (MQ=255)

                                                                                                                                                                                                                             aaCATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGccc                                                                                                                                                   >  6:265220/1‑149 (MQ=255)

                                                                                                                                                                                                                                   cTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCAAGGTCAGGGAAATCTTATCGAAGATGCAGCGATTGCCAAGCGTAAAACTGACATCGCGATTATAGAATCAATTCTCCACACCCTGCCACACAATTCACCATTc               >  4:274836/1‑275 (MQ=255)

                                                                                                                                                                                                                                      aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg                                                                                                                                                                                                   <  8:61186/92‑1 (MQ=255)

                                                                                                                                                                                                                                      aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTg                                                                                                                                                                                                   >  7:61186/1‑92 (MQ=255)

                                                                                                                                                                                                                                      aTCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc                                                                                                                                          >  5:307493/1‑149 (MQ=255)

                                                                                                                                                                                                                                          gTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTTACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg                                                                                                                                      <  5:429885/149‑1 (MQ=255)

                                                                                                                                                                                                                                            ttGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGAt                                                                                                                                    <  7:84713/149‑1 (MQ=255)

                                                                                                                                                                                                                                                gcACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGGCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTAAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAt  <  2:53217/275‑1 (MQ=255)

                                                                                                                                                                                                                                                gcACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTc                                                                                                                                >  7:302990/1‑149 (MQ=255)

                                                                                                                                                                                                                                                    tGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCg                                                                                                                                                                                               >  7:169630/1‑82 (MQ=255)

                                                                                                                                                                                                                                                     gTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTcg                                                                                                                           >  6:98535/1‑149 (MQ=255)

                                                                                                                                                                                                                                                         aCAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGAt                                                                                                                                                        <  2:45622/116‑1 (MQ=255)

                                                                                                                                                                                                                                                          cAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCa                                                                                                                      >  5:371349/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           aG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCAc                                                                                                                     <  6:348281/149‑1 (MQ=255)

                                                                                                                                                                                                                                                           aG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCAc                                                                                                                     >  8:185536/1‑149 (MQ=255)

CCGGCTCCAGCGCAATCGCACGCGCCAGCGCTGCACGCCGCGCCATCCCACCGGAAAGTTCAGAAGGCATTAGTTTAGCCGCTCCACGCAGCCCCACGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAG‑GCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAT  >  NC_000913/3339576‑3340090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`