Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,560,455:1	+G	100%	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	96.7%	314.4 / ‑1.4	89	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base G (43/43);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATC  >  NC_000913/3560181‑3560709                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                               gCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑c                                                                                                                                                                                                                                                                 <  4:157311/275‑1 (MQ=255)  ccACCGGCGAGAATGATGCTAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGGGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑c                                                                                                                                                                                                                                                                 <  2:342864/274‑1 (MQ=255)                  tgCGAAATTCTTCTTTTACCATCATCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCTCAAATTGCTGTGATTGGGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                                <  2:210769/275‑1 (MQ=255)                       aaaTCTGCTTTTGCAATCAAGGTGTTAGCAACGTTGTGATTGTTGGGTAAAAGGCGCAAATTGCTGTGATTGAGGAGTGCGGGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCg                                                                                                                                                                                                                                            <  4:353605/274‑1 (MQ=255)                                  tACCATCAACGTGTTGGCAACGTTGGGATTGTTGGTGACAATGCGCAATTTGCTGTGATTGGGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTTCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑cgc                                                                                                                                                                                                                                                               <  4:330899/244‑3 (MQ=255)                                    ccATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTGTGCTCCGCCAGCCCATTGAgg                                                                                                                                                                                                                              >  2:279694/1‑275 (MQ=255)                                      aTCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                                            <  2:197992/275‑1 (MQ=255)                                                      cGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCTATTGAGGTCGCGGCGAATAGTCTGc                                                                                                                                                                                                            >  2:264748/1‑275 (MQ=255)                                                         tGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                                          >  2:74558/1‑274 (MQ=255)                                                                 ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGga                                                                                                                                                                                                 >  2:184021/1‑275 (MQ=255)                                                                  tggtggCAATGGGCAAAGTGCTGGGATTGGGCAGTGCGGGCTCTACCGTGTCCGGCGGGGTGCGGATATCGTTAAACTGCTTCGTGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag                                                                                                                                                                                                <  4:339538/275‑1 (MQ=255)                                                                  tgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTCCCCGAACGGGCAGGCAGCGCAGCACCGCCCTGAAT‑GC‑G‑CAGGATCGGGTTTTGCCCCGTGAGCTCATTGAGGTTGCGGAGAGTCGTCTGCTGGCTGAgggg                                                                                                                                                                                                 >  4:18531/1‑273 (MQ=255)                                                                    gTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACCGCGTGTTAACCGAACTGGAAAGCAGCGCCGCACCTCCCTG‑AT‑GG‑CGCAGGATCCGGTTTGGCGACGCCAGCCCATTCAGGCACCGCCGAATAGTCTGCGGGCTGTCgtaca                                                                                                                                                                                               >  4:288779/1‑270 (MQ=255)                                                                        aaaTGGGCAAATGGCTGGGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                                                                                                                                           <  2:18531/273‑1 (MQ=255)                                                                           tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                                        >  1:86598/1‑274 (MQ=255)                                                                           tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGGATAGTCTGCGGGCTGAc                                                                                                                                                                                                    >  2:339542/1‑262 (MQ=255)                                                                                       tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGACGGCAGCGCCGCACCGCCATG‑CT‑GG‑CGCAGGATCAGCTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCACctctcc                                                                                                                                                                            >  4:93888/1‑272 (MQ=255)                                                                                           aTTGAGCAGTGCGTGCGCTACCGCTTCCGGGGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                                        <  1:117221/274‑1 (MQ=255)                                                                                            ttGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACt                                                                                                                                                                      >  2:433709/1‑275 (MQ=255)                                                                                            ttGAGCAGGGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                       <  2:288783/274‑1 (MQ=255)                                                                                                gCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGAACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa                                                                                                                                                                  <  2:8605/275‑1 (MQ=255)                                                                                                    tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGTAGTCTGCGGGCTGACGGAGACATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                                              >  2:412370/1‑275 (MQ=255)                                                                                                     gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGACAAACAGCGTCGAGCCATTGCGGATTTGCTCAGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGCGCCACGGCGGGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGTTAAGGTTTTGCTCGGCCACCCGATTTCGCCCGCGGCGACTAGTCTGCGGGCTGAAGGAGCACTGCACTACCCGTCCTTCGTGACCGACAttaac                                                                                                                                                             >  4:246243/1‑271 (MQ=255)                                                                                                         ccgcTACCGCTTCCGGCCTGGTGCCGTTATCGATAACCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                       <  2:246247/260‑1 (MQ=255)                                                                                                              aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAATGGGGATTTGCTCAGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACGGCCATT‑AT‑GG‑CGCAGGATCAGGTTTTGTTCCGCCAGCGGATATAGGTCGAGGAGAATAGTCTGCGGGCt                                                                                                                                                                                                       >  1:40771/1‑224 (MQ=255)                                                                                                                  nttCCGGCGTGTTGNCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                                                                                                                                  <  4:522485/208‑1 (MQ=255)                                                                                                                             gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                                                   >  2:271743/1‑213 (MQ=255)                                                                                                                                        gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCa                                                                                                                                                                                                                                                        >  7:218279/1‑149 (MQ=255)                                                                                                                                         aTAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                                                                                                                                                      >  4:72978/1‑214 (MQ=255)                                                                                                                                          tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTTCGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGTTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                         <  2:478801/274‑1 (MQ=255)                                                                                                                                          tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                                         <  2:93889/274‑1 (MQ=255)                                                                                                                                            aaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGtt                                                                                                                                                                                                                                                    <  7:205587/149‑1 (MQ=255)                                                                                                                                             aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGttt                                                                                                                                                                                                                                                   >  7:254336/1‑149 (MQ=255)                                                                                                                                             aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                  <  2:72979/214‑1 (MQ=255)                                                                                                                                                 cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCt                                                                                                                                                                                                                                               <  5:54112/149‑1 (MQ=255)                                                                                                                                                 cGTCGAGCCATTGGGGATTTGCTCCGCCAATTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGTGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATAACCCTGCTTTTTAACCAGTTCGATAATACCGTTGTGTCGTTGtgttt                                                                                                                 >  1:123532/1‑275 (MQ=255)                                                                                                                                                   tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTAGGTGGCCTTGCAATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTcc                                                                                                                                                                                                                                             <  7:330177/149‑1 (MQ=255)                                                                                                                                                             ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                                                   >  5:131776/1‑149 (MQ=255)                                                                                                                                                              gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                                  >  5:159046/1‑149 (MQ=255)                                                                                                                                                              gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                                  <  5:212187/149‑1 (MQ=255)                                                                                                                                                                ggtttCCTCGGCCACGTTGCGGGCGGTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAATGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtata                                                                                                     <  2:183974/270‑1 (MQ=255)                                                                                                                                                                 aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                                               >  5:330978/1‑149 (MQ=255)                                                                                                                                                                 aTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                                                                                                                                               <  6:55348/149‑1 (MQ=255)                                                                                                                                                                     gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                                           >  5:456507/1‑149 (MQ=255)                                                                                                                                                                           ccACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTc                                                                                                                                                                                                                                     >  1:27260/1‑133 (MQ=255)                                                                                                                                                                             aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                                   >  8:127109/1‑149 (MQ=255)                                                                                                                                                                              cTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                                                                                                                                  <  5:36228/149‑1 (MQ=255)                                                                                                                                                                               tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                                 <  3:27260/133‑1 (MQ=255)                                                                                                                                                                                         aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTAGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                                         >  1:94272/1‑243 (MQ=255)                                                                                                                                                                                         aTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGACAAGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GGCC‑CAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAACAGCTCTTCGGTACTGACATAACCCTGCTGGTTAACCAGTTCGATAACACCCTTGTGAAGTTACGTTTGTTTCATTCATAAATCCTGTGAATTCttctgtttttcgag                                                                         >  4:111250/1‑273 (MQ=255)                                                                                                                                                                                                 tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                                 <  6:376415/115‑1 (MQ=255)                                                                                                                                                                                                 tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                                 >  5:376415/1‑115 (MQ=255)                                                                                                                                                                                                  gtttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                                                                                                                                              <  6:377006/148‑1 (MQ=255)                                                                                                                                                                                                    ttcttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                                                                                                                                            <  8:254336/149‑1 (MQ=255)                                                                                                                                                                                                      tttcggcgcgcgTGGCCTTGGGAAGGGGCGAGGGCGTGTTAACCGAACTGTAAGGATGGGCAGCACCGCAAGT‑ATGGG‑G‑CAGGTTCAGGTTTTGCTACGCAAGCTCATTGAGGTGGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                                             <  4:433704/263‑1 (MQ=255)                                                                                                                                                                                                      cttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                                                          >  7:422835/1‑149 (MQ=255)                                                                                                                                                                                                      ctgtcgcggtgggggCCGTGCGACGGTGGCACGGGGGATTAACCGAACTGGACGGGAGCGCGGGCCCGCGCTG‑AT‑GG‑CGAAGGATCGGGTTTTGCTCCGCCAGATCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGCTCTTCTGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAAGACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                                             <  4:70402/261‑1 (MQ=255)                                                                                                                                                                                                       ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                                                         >  5:208963/1‑149 (MQ=255)                                                                                                                                                                                                       ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct                                                                                                                                                                                         >  8:144036/1‑149 (MQ=255)                                                                                                                                                                                                          ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTa                                                                                                                                                                                      <  6:456507/149‑1 (MQ=255)                                                                                                                                                                                                           gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                                                                                                                                                >  1:20849/1‑106 (MQ=255)                                                                                                                                                                                                           gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaaa                                                        >  1:14375/1‑272 (MQ=255)                                                                                                                                                                                                               gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                                            <  3:20849/106‑1 (MQ=255)                                                                                                                                                                                                               gggTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc                                                                                                                                                                                 >  8:52423/1‑149 (MQ=255)                                                                                                                                                                                                                   ggCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                             <  6:159046/149‑1 (MQ=255)                                                                                                                                                                                                                    gCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                                            <  6:323697/149‑1 (MQ=255)                                                                                                                                                                                                                     ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                                                                                                                                           <  6:131776/149‑1 (MQ=255)                                                                                                                                                                                                                     ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                                                        >  1:115389/1‑232 (MQ=255)                                                                                                                                                                                                                      cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                                                                                                                                          <  8:114645/149‑1 (MQ=255)                                                                                                                                                                                                                          cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACt                                                                                                                                                                      <  5:241895/149‑1 (MQ=255)                                                                                                                                                                                                                          agATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGACGCACAGCCCAT‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCACGCTCATTGAGGTAGCGGCGAATAGTCTGCGGGTCGACGGAGTAATGCTCTACCAGCTCTTCGGTACCGACNTAACCATGCTGTTGAAACAGTTTGGTCACACCGTTGTACCGTAGtgttnnt                                                                                                               >  4:508338/2‑204 (MQ=255)                                                                                                                                                                                                                               gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                                                                                                                                        >  4:426171/1‑94 (MQ=255)                                                                                                                                                                                                                               gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                                                 >  6:395146/1‑149 (MQ=255)                                                                                                                                                                                                                                   cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                                                                                                                                    <  2:426176/94‑1 (MQ=255)                                                                                                                                                                                                                                    aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                                  >  6:269032/1‑79 (MQ=255)                                                                                                                                                                                                                                    aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                                  <  5:269032/79‑1 (MQ=255)                                                                                                                                                                                                                                         gtgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg                                                                                                                                                       >  6:43791/1‑149 (MQ=255)                                                                                                                                                                                                                                          tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                                                                                                                                                           >  4:303688/1‑128 (MQ=255)                                                                                                                                                                                                                                              aaCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                       <  2:303692/128‑1 (MQ=255)                                                                                                                                                                                                                                               aCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                                 <  7:52423/149‑1 (MQ=255)                                                                                                                                                                                                                                               aCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                                 >  8:137564/1‑149 (MQ=255)                                                                                                                                                                                                                                               aCCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                                                 <  6:208963/149‑1 (MQ=255)                                                                                                                                                                                                                                                     cTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                                                                                                                                           <  7:127109/149‑1 (MQ=255)                                                                                                                                                                                                                                                     cTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTACCCCGTCTATGTGTGCTc             >  2:81776/1‑275 (MQ=255)                                                                                                                                                                                                                                                       ggAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGa                                                                                                                                         <  8:218279/149‑1 (MQ=255)                                                                                                                                                                                                                                                                cgccgcACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGATTTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATTGCTAAACCCACGCCTAACCCGCCCATCTGTTCTCCGTTCGCCTTc  >  2:33152/1‑275 (MQ=255)                                                                                                                                                                                                                                                                 gccgcACCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                                               <  8:422835/149‑1 (MQ=255)                                                                                                                                                                                                                                                                      aCCGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                                             <  2:489837/210‑1 (MQ=255)                                                                                                                                                                                                                                                                        cGCCATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa                                           >  2:286854/1‑226 (MQ=255)                                                                                                                                                                                                                                                                          ccATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                                      >  8:78199/1‑149 (MQ=255)                                                                                                                                                                                                                                                                           cATG‑AT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                                                     <  7:144036/149‑1 (MQ=255)                                                                                                                                                                                                                                                                            atg‑at‑gG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc                                       <  4:286850/226‑1 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                               GCCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATG‑AT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATC  >  NC_000913/3560181‑3560709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑