| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,318,095 | C→G | 100% | L594F (TTG→TTC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,318,095 | 0 | C | G | 99.6% | 297.0 / ‑6.7 | 86 | L594F (TTG→TTC) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base G (41/42); minor base T (2/0); total (44/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAA > NC_000913/2317827‑2318325 | aCTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGTCGGTATCCCCACGCCGGTATCGCGAACACGGCTAGCTAGATAACCCCAATCCCCGCGAACACGCAAAAACTCACAGCCGGTATCGTTGAATTTTATGTCGTTACACAACAg > 4:211382/1‑274 (MQ=255) ttCCCGGTTCTGAAGCTACCGAGATATCGCCGGCCAGCATGCTGATCAGTGTGTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAc < 2:125984/274‑1 (MQ=255) cGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCggg > 2:166048/1‑274 (MQ=255) gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTa > 2:139570/1‑275 (MQ=255) aTCAGTTTTCCACAAATCGCCAGAGCCAGACCGGTCCCCTGGAAATTACGCTGGACGCCCGTCCCGACCTGGCAGAAGCCATCAAACAACCGTACTACTTATTTCACCGGTATCCCAACGCCGGTATCGCGAACACGGATAGAGTGATAAACGCTATCCGCGCGCACAATAAAAACTATACACCCGCTCTCGCACATTTCTATGGCGATACTGCACTCGTCGCCGCCGCCTTGCTGTAAAGCGCA‑CGTGCCCCCATCTAAGGCCACTGTcaacac > 4:154343/1‑271 (MQ=255) ttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa < 2:488536/275‑1 (MQ=255) ttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCaa < 2:211385/275‑1 (MQ=255) ttCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGACATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGtt < 2:421452/272‑1 (MQ=255) tgagtccggacacagaccGGTCCCACGTGAATTACGAGGTCATCCCGGTCGGACCAGGAAGCAGGGATCAAACAAGCGCATGCCTGCTTTTGCCGGTATCCCGAAGCCTGTATCGCTAACACGCATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGTTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑TGCATGGTGTCGCCATGTAAGGCCACTTGCACATCCGGTTCaagcaag < 4:350217/266‑6 (MQ=255) cagacccaggccGGTCCCCTGGAAATTCCGCTGTACGCCCGGTCCGACCGTGAAGAAGGGATCAAACCAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGATATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAc < 1:37296/274‑1 (MQ=255) gTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGACGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGCACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGACCAGGTTGGAGATGACCTGCTGTAAA‑CGCATAGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAt > 2:350222/1‑251 (MQ=255) tCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTAACGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCCTCCGGTCGCCATTTAAGGCCACTGGCAAAACCGGTTCAAACAAACAGTACAACCCTAACTGCttc > 4:241213/1‑274 (MQ=255) attaagctctcagCCCGTTCCGACCTTGGAGAATGGATCAAACAAGCGCACCACTTCTTTCGCCGTGATACCCACGCCGGTATCGCGAACACTGATAGAGAGATAAAAGCCATCGGCGCGAACATGCAAAAGTATACAGCCGGTATCGGGGAATTTTATGGCGTTACTGAACAGGTTGGAGAGGACCTGCAGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTaccac < 4:302813/261‑1 (MQ=255) aTTACGCTGTACGCCCGTTCCGACCTGGAAGAATGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAGCAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTAccacca < 1:113974/275‑1 (MQ=255) cGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTTAACAGGTTTGAGATGACCTGCTGTAAA‑CGCAANGGGTCGCCCTTTACGGCAACTGGCACATCCGGTTCAATAAAGCAGTACAACCCTAAATCATTGCGTTACACCCGctg > 3:110538/1‑273 (MQ=255) gcgtgaCGTCCGTTCCGCCCTGGAAGAAGGGATCAAACAAGCGCCCCACCTCCTGTGCCGGTAGCCCCGCGCCGACATAGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTAGGGCGTTACTGAACAGCTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCAATGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGt < 4:166337/270‑1 (MQ=255) ccGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCgg > 2:366421/1‑274 (MQ=255) ccTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAAc > 5:461151/1‑149 (MQ=255) ggAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAgg > 5:226437/1‑149 (MQ=255) agaagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTg < 6:411272/149‑1 (MQ=255) gaagGGATCTAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTGATGGCGTTACTGAACAGGTTgg > 5:408996/1‑149 (MQ=255) gaagGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTgg > 7:109532/1‑149 (MQ=255) gATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATg > 8:394012/1‑149 (MQ=255) aTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGa < 5:225999/149‑1 (MQ=255) aTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGa > 7:385208/1‑149 (MQ=255) aTCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGa < 8:445879/149‑1 (MQ=255) cAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGAcc > 5:239775/1‑149 (MQ=255) aCAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAAGTTGGAGATGACctgc > 6:359359/1‑149 (MQ=255) aaGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt < 6:233284/133‑1 (MQ=255) aaGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGt > 5:233284/1‑133 (MQ=255) aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGt < 6:295857/149‑1 (MQ=255) aGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGt < 6:408996/149‑1 (MQ=255) cgcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCCAACACGGATAGATAGATAATCTCCATCCGCCCGAACATGCAAAACTATACAGCCGGTATCGGCGAACTTTATGGCGTTTCTGAACCGGCTGGAGATTACCTGCTGTAAA‑CGCATCGGTTCGCCATTTAACGCCACTGGTCCATCCGGTGCAATAAGGCAGTACCAGCTTAACTGCTTGCGTACCACCAGCGGTAAAGAGTTGGCGGGTACGTGGTCCATCACTTC‑CGCgggtga > 4:467397/1‑271 (MQ=255) gcACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTaaa < 6:461151/149‑1 (MQ=255) caccacTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑c > 7:242181/1‑149 (MQ=255) cgtcttTCGCCGGTATCCCCACGCCGGTATCACGAACACGGATAGAGGGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGCCCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCa < 1:110538/273‑1 (MQ=255) ttcttTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCg > 6:417863/1‑149 (MQ=255) tttCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGGTGTAAA‑CGCATCGGGt < 8:177003/149‑1 (MQ=255) tttCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGt > 7:454612/1‑149 (MQ=255) gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCAttt < 8:385208/149‑1 (MQ=255) gTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATc < 2:241217/275‑1 (MQ=255) aTCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTaa < 6:429542/149‑1 (MQ=255) aTCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTaa < 6:239775/149‑1 (MQ=255) tCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAg < 5:21496/149‑1 (MQ=255) tCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAg < 8:242181/149‑1 (MQ=255) cccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGc > 8:249229/1‑149 (MQ=255) cccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGc > 7:439250/1‑149 (MQ=255) ccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAAGATGCAAAACTATACAGCCGGTATCGGTGAATTTTATAGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTAAAAACCTAACTGCTTGGTGACCCACAACCGAAAATAGTTGGCGGTGATGCGGTCAATCACTTCACGGGGGTAAAACCCCGACGGATCGATCTTCCAc > 4:329731/1‑275 (MQ=255) ccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGcc > 5:226193/1‑149 (MQ=255) ccACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGcc > 5:112360/1‑149 (MQ=255) cACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCa > 5:443620/1‑149 (MQ=255) cGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGc < 6:443620/149‑1 (MQ=255) tATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTGTAACTGTTCCGAt > 2:273379/1‑275 (MQ=255) acacGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTc < 7:394012/149‑1 (MQ=255) ggATAGGGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATa < 6:119676/149‑1 (MQ=255) tAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg < 8:192218/149‑1 (MQ=255) tAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa < 6:226193/149‑1 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACATCCGGTATCGGTGAATTTTTTGGCGTTACTTAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATGGGGTCCCCATTTAAGGCCACTGGCACATCCGGATCAATAAAGGAGTACAAGCATAACTGCTTGAGTACCACCAGCGGCAAAGAGTTGGCGGTGATGTAGTTCATCACGTCACGCGGTGTAAACTCACGCTGATCGATCTTACACGCTTCCGTGTCCATCTTCGAGAAATCGaaaaat > 4:155724/1‑273 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa > 8:279541/1‑148 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACaa < 7:279541/148‑1 (MQ=255) aaTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAg < 6:145168/149‑1 (MQ=255) aTCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGc < 6:226437/149‑1 (MQ=255) gCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGc > 4:349545/1‑275 (MQ=255) tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTaa < 8:378713/104‑1 (MQ=255) tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTaa > 7:378713/1‑104 (MQ=255) tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCCACTTTTCCGATTCAATCTTCGAGCAATCGAGAATATCGCTGAt > 1:110532/1‑275 (MQ=255) tCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGCAAAATCGAAAATATCggctg > 2:491648/1‑270 (MQ=255) gcgcgAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCtt > 5:385497/1‑149 (MQ=255) aaCATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCAAATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGATCATCACTTCACGCGGTGAAAACTCACGCGGTTTGATCTTCAACTGCTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATATTTTTca > 2:256214/1‑275 (MQ=255) aacAAAACTTTGCTTCCGGTATCTGTGAAGTTTATGGCGTTGCTGAACAGGTGGGAGAAGACCAGCTGGTAA‑TGCTGCGAGACGCCATTTAAGGACACTTGCACATCCGATTCAATAAAGTAGTTCAAGCCTAACTGCTTGCGTTCCACCAGCGGTAAATAGTTTGCAGGGATGTGGTTTATCGCTTTACGCGTTGAAAACACACGCGGTTCGATCTTCAACTGTTCCGAGTCAATCTGCGAGAAATCGAGAATATCGCGTTTAATTTGcaacaa < 4:256210/273‑1 (MQ=255) ataCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAAt > 3:29409/1‑107 (MQ=255) taCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAAGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTaaa < 5:359359/149‑1 (MQ=255) aCAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAAt > 6:187808/1‑149 (MQ=255) cAGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATa > 5:396805/1‑149 (MQ=255) aGCCGGTATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAg < 1:29409/107‑1 (MQ=255) tATCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCg < 8:109532/149‑1 (MQ=255) tCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGt < 8:175311/149‑1 (MQ=255) tCGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGt > 6:401705/1‑149 (MQ=255) cGGTGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTg < 5:187808/149‑1 (MQ=255) tGAATTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAAc > 4:61340/1‑176 (MQ=255) aaTTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGAtgtg < 7:249229/149‑1 (MQ=255) aTTTTATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTgg > 7:134680/1‑149 (MQ=255) ttttATGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCAc < 2:61341/176‑1 (MQ=255) aTGGCGTTACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTtcat < 6:396805/149‑1 (MQ=255) ttACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACtt > 4:50747/1‑148 (MQ=255) tACTGAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTGGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCa > 7:292195/1‑149 (MQ=255) | ACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTATACAGCCGGTATCGGTGAATTTTATGGCGTTACTCAACAGGTTGGAGATGACCTGCTGTAAA‑CGCATCGGGTCGCCATTTAAGGCCACTGGCACATCCGGTTCAATAAAGCAGTACAAGCCTAACTGCTTGCGTACCACCAGCGGTAAATAGTTGGCGGTGATGTGGTTCATCACTTCACGCGGTGAAAACTCACGCGGTTCGATCTTCAACTGTTCCGATTCAATCTTCGAGAAATCGAGAATATCGCTGATAATTTTCAACAA > NC_000913/2317827‑2318325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |