BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,781,051	C→G	11.2%	intergenic (+34/‑164)	lldD → / → trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,051	0	C	G	11.2%	959.4 / 87.3	289	intergenic (+34/‑164)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base C (153/103); new base G (12/20); total (165/124)
Fisher's exact test for biased strand distribution p-value = 2.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-03

Only 100 of 292 total aligned reads displayed.

CGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGG  >  NC_000913/3780785‑3781320

 gcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCCGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCACCCCTGGCGAAAGGGACTGAGGCATAGTCGTTTGCGCCCCCTCCCCCCAACCC‑CCCCCCCAGGGGCAggg                                                                                                                                                                                                                                                                       >  4:11744/1‑275 (MQ=255)

    cGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgggt                                                                                                                                                                                                                                                                      <  2:11744/274‑2 (MQ=255)

       tGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCCCAGGGAGAGGGGACCg                                                                                                                                                                                                                                                                  >  2:62431/1‑275 (MQ=255)

                acagctgctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                                                                                                                                                          <  2:506159/270‑1 (MQ=255)

                               tGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                                                                                                                                                                                        >  2:92614/1‑261 (MQ=255)

                               tGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGCAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTATCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTc                                                                                                                                                                                                                                          >  2:152175/1‑274 (MQ=255)

                               tGCTTTCCTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGC‑ATGTACctcctc                                                                                                                                                                                                                                          >  1:104299/1‑274 (MQ=255)

                                   ttCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgt‑atg                                                                                                                                                                                                                                                   <  4:92613/261‑1 (MQ=255)

                                              gcTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                                                                                                                                                                                    >  2:396958/1‑234 (MQ=255)

                                               cTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                                                                                                                                                                           <  2:180442/242‑1 (MQ=255)

                                                tggaacaggCGGGGCAGGGTGGTGTACGTAACCTGCTAAATCTGATCGAAAAAGAGACGAAGGGGGGGGTGAGTGGGACTGGAGCGAAAAGGAACGGCGAAATTACGCAAGATTGGCTGGTGAAGGGGGGGGGTAAAGAGTTGCCTGCGGCACTGGATCCAATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCGCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTGCGGCGATGT‑ATGGACTCCCTCAC‑TCTGAAACGACACCGc                                                                                                                                                                                                                       <  4:502351/267‑1 (MQ=255)

                                                 ggaaaCAGCGGGCCAGGCGGGTTTTGCTAACCTGCGAAAACTGATCGAAAAAGAGATGAAAGTGGCGATGACTCTGACGGGCGCGAAATCGATCAGCGAAATTCCGGAAGATTCGCTGGTTCAGGGGCTGGGTAAAGAGTGGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCa                                                                                                                                                                                                                      <  4:406081/272‑1 (MQ=255)

                                                                   gggTGTAGCTAACCTGCTAAATCTGATCGAAAAAGGGATGAAAGGGGAGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCACTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                                                                                                                                                    <  2:411689/275‑1 (MQ=255)

                                                                   gggTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                                                                                                                                                    <  2:206913/275‑1 (MQ=255)

                                                                               ccTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCAACCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                                                                                                                                                              >  2:138985/1‑207 (MQ=255)

                                                                                   cTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgt‑atgt                                                                                                                                                                                                                                                  <  1:50717/214‑1 (MQ=255)

                                                                                   cTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACCCCCTCAC‑TCTGAAACGAAACCGCCCTCTTTTTTTCTCCCTCGCCCCTCCGGGGGGAgg                                                                                                                                                                                    >  1:8370/1‑275 (MQ=255)

                                                                                           gATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCACAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTTAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                                                                                                                                                             >  1:19299/1‑274 (MQ=255)

                                                                                             tCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCCCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACCCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCTCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                                                                                                                                           >  2:267346/1‑274 (MQ=255)

                                                                                                          gAAAGGGTCGATGAGGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGga                                                                                                                                                                                                                                                                            <  4:357252/166‑1 (MQ=255)

                                                                                                           aaaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGc                                                                                                                                                            >  2:484603/1‑275 (MQ=255)

                                                                                                                      gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTc                                                                                                                                                                                                                                                                                 <  7:456090/149‑1 (MQ=255)

                                                                                                                        cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACCGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                                                                                                                                                               <  8:33303/149‑1 (MQ=255)

                                                                                                                         gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                                                                                                                                                                              <  8:432647/149‑1 (MQ=255)

                                                                                                                         gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGTTGCGGGGGAAAGGCCGCACTGCTCACCAAt                                                                                                                                               >  2:246771/1‑274 (MQ=255)

                                                                                                                         gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGACGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                                                                                                                                                                              <  6:388609/149‑1 (MQ=255)

                                                                                                                              cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGagag                                                                                                                                                                                                                                                                         <  6:114736/149‑1 (MQ=255)

                                                                                                                               tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                                                                                                                                                        <  8:179230/149‑1 (MQ=255)

                                                                                                                                   gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                                                                                                                                                                                    >  7:168754/1‑149 (MQ=255)

                                                                                                                                         tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                                                                                                                                                              <  5:426136/149‑1 (MQ=255)

                                                                                                                                           gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                                                                                                                                                        <  5:331226/137‑1 (MQ=255)

                                                                                                                                           gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                                                                                                                                                        >  6:331226/1‑137 (MQ=255)

                                                                                                                                            aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                                                                                                                                                                                    >  1:128402/1‑140 (MQ=255)

                                                                                                                                               aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                                                                                                                                                        <  5:188680/133‑1 (MQ=255)

                                                                                                                                                  gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCATGGAGAGGGGACCGTTCGGCGCtgt‑at                                                                                                                                                                                                                                                    <  7:296140/149‑1 (MQ=255)

                                                                                                                                                   aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgt‑atg                                                                                                                                                                                                                                                   <  8:217442/149‑1 (MQ=255)

                                                                                                                                                   aaaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgt‑atc                                                                                                                                                                                                                                                   <  6:98075/149‑2 (MQ=255)

                                                                                                                                                     aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                                                                                                                                                       >  6:228453/1‑128 (MQ=255)

                                                                                                                                                     aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCACCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgt‑atgta                                                                                                                                                                                                                                                 <  7:126604/149‑1 (MQ=255)

                                                                                                                                                              gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATg                                                                                                         >  2:438566/1‑275 (MQ=255)

                                                                                                                                                                ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAc‑tc                                                                                                                                                                                                                                     >  7:233260/1‑149 (MQ=255)

                                                                                                                                                                    cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAggg                                                                                                                                                                                                                                                                       <  6:221393/113‑1 (MQ=255)

                                                                                                                                                                      ggTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACa                                                                                                  >  2:345051/1‑274 (MQ=255)

                                                                                                                                                                          cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAggg                                                                                                                                                                                                                                                                       >  5:60481/1‑107 (MQ=255)

                                                                                                                                                                                 tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGCGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                                                                       >  2:309201/1‑274 (MQ=255)

                                                                                                                                                                                  gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCActc                                                                                                                                                                                                                   >  7:426688/1‑149 (MQ=255)

                                                                                                                                                                                   ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                                                                                                                                                       >  6:191611/1‑98 (MQ=255)

                                                                                                                                                                                    gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCtt                                                                                                                                                                                                                 <  5:343221/149‑1 (MQ=255)

                                                                                                                                                                                      aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCtttt                                                                                                                                                                                                               <  8:164716/149‑1 (MQ=255)

                                                                                                                                                                                       aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCttttt                                                                                                                                                                                                              <  5:135075/149‑1 (MQ=255)

                                                                                                                                                                                       aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGACGGGGGGAGGGGAAAAGGCCGCACTGACAACCAATATTGTGCTATACTGCCCCCGCACTACGGGGGCAGTATGCCAAACATCTTAATTTACGAAcaa                                                                                 >  1:64479/1‑272 (MQ=255)

                                                                                                                                                                                        agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCtttttt                                                                                                                                                                                                             >  5:247546/1‑149 (MQ=255)

                                                                                                                                                                                             tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAAATCGTACTTTACGAACCAGAAATTc                                                                          >  2:2232/1‑275 (MQ=255)

                                                                                                                                                                                               ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAgg                                                                                                                                                                                                                                                                              >  7:305287/1‑79 (MQ=255)

                                                                                                                                                                                                cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGagag                                                                                                                                                                                                                                                                         <  8:66224/83‑1 (MQ=255)

                                                                                                                                                                                                 tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                                                                                                                                                               <  4:533864/76‑1 (MQ=255)

                                                                                                                                                                                                  gCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                                                                                                                                                                                       <  4:263777/161‑1 (MQ=255)

                                                                                                                                                                                                  cgggcatcgtcTCCCAGGGCGAAAGGGAATCGGGTAGTGT‑GCGTGCGCCCCCCCCTCCCAACCCCCCCCCCTGGGGGGGGGGGACCGGTGGGGGCCGTAAGGTAC‑CCCCCACTTCTGAACCGACCCCTAATTCTTTTTTGCCCCCCCCCCCCTCCGGGGGGGGGGCGGGGGGGGGGGCAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc                                                                      <  4:212308/264‑1 (MQ=255)

                                                                                                                                                                                                      cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                                                                                                                                               >  6:301514/1‑149 (MQ=255)

                                                                                                                                                                                                      cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                                                                                                                                               >  5:299373/1‑149 (MQ=255)

                                                                                                                                                                                                             tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGCGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                                                          >  5:195214/1‑147 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                                                                                                                                                                                        <  7:329116/69‑1 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGGGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      >  6:5641/1‑149 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      <  8:191362/149‑1 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      >  8:122989/1‑149 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      >  7:338684/1‑149 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      <  7:187440/149‑1 (MQ=255)

                                                                                                                                                                                                               ccATGGCGAAAGGGAATGCAGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      <  5:450127/149‑1 (MQ=255)

                                                                                                                                                                                                                 aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgg                                                                                                                                                                                    <  6:172932/149‑1 (MQ=255)

                                                                                                                                                                                                                     cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                                                                                                                <  6:285353/149‑1 (MQ=255)

                                                                                                                                                                                                                      gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTg                                                                                                                                                                                                                                   <  8:296442/97‑1 (MQ=255)

                                                                                                                                                                                                                       aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                                                                                                                                                                                         <  6:444454/60‑1 (MQ=255)

                                                                                                                                                                                                                       aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                                                              <  6:476373/149‑1 (MQ=255)

                                                                                                                                                                                                                           ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                                                          <  5:199521/149‑1 (MQ=255)

                                                                                                                                                                                                                           ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                                                          <  8:248990/149‑1 (MQ=255)

                                                                                                                                                                                                                           ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                                                                                                          >  8:363488/1‑149 (MQ=255)

                                                                                                                                                                                                                           ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGa                                                                                                                                                                          >  8:159688/1‑149 (MQ=255)

                                                                                                                                                                                                                                     cgatctgTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGTTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGGGAGGGCCGGGGTGAGGGGAAAAgg                                                                                                                                                                <  7:269818/143‑1 (MQ=255)

                                                                                                                                                                                                                                            tttGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                                                                      <  5:22154/120‑1 (MQ=255)

                                                                                                                                                                                                                                            tttGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCCGT‑ATGTACCCCCCCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCCCCGGGGAGAGGGGCGGGGGGGGGGGAAAAGGcagatcg                                                                                                                                                         >  8:269818/1‑143 (MQ=255)

                                                                                                                                                                                                                                              tGCGCCCCCTCACCCTAACCCTCCCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTc                                                                                                                                                                                                                                          >  2:132772/1‑67 (MQ=255)

                                                                                                                                                                                                                                                cgcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTc                                                                                                                                                     >  5:463296/1‑149 (MQ=255)

                                                                                                                                                                                                                                                 gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCa                                                                                                                                                    >  6:184445/1‑149 (MQ=255)

                                                                                                                                                                                                                                                 cccccccccccCTCCCCCCCCCCCCCGGGGAGGGGGCACCGTCCGGCCCTTT‑ATGCCCCCCCCCCC‑TCGAAACCGACCCCCCCCTCTTTTTTCCCCCCCCCCCCCCCGGGGGGGAGGGCGGGGGTGGGGGAAAAAGGCCGCACTGCTCa                                                                                                                                                    <  6:195922/148‑1 (MQ=255)

                                                                                                                                                                                                                                                   ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAcc                                                                                                                                                  >  8:314987/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                                                                                                     >  7:68662/1‑106 (MQ=255)

                                                                                                                                                                                                                                                                 ccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGTGAGGGCCGGGGTGAGGGGAAATGGCAGCACTGCTCACCAATTTTCTGATATc                                                                                                                                    >  5:161664/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                  ctctCCCTGAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                                                                       <  7:37595/113‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                                                                                                                                                >  7:5006/1‑120 (MQ=255)

                                                                                                                                                                                                                                                                     tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcccc                                                                                                                                                                                                >  2:258178/1‑85 (MQ=255)

                                                                                                                                                                                                                                                                     tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa                                                                                                           >  2:339885/1‑170 (MQ=255)

                                                                                                                                                                                                                                                                     tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCACATCCTGGCAACATCATCCGTCTTTGCGCTAATACCCGCTTTCGTCTGCATACAATCGAACCGATg   >  2:7933/1‑274 (MQ=255)

                                                                                                                                                                                                                                                                     tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATgg  >  2:361386/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                     tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATgg  >  2:101082/1‑275 (MQ=255)

                                                                                                                                                                                                                                                                     ccccTCAGGGAGAGGGGACCGTTTGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCTCGCACGAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAACAATTCCGCCAAATCATGGCAACATCATCCGTCTTTGCGCTAATACAGGATTTCGTCGGCATCAGCTCAAACCGATgg  >  4:13915/2‑275 (MQ=255)

                                                                                                                                                                                                                                                                       ccTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                                                                                              >  8:39899/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                        cTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                                                                                               <  5:270095/99‑1 (MQ=255)

                                                                                                                                                                                                                                                                         tCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATccagatcgg                                                                                                                            >  7:160554/1‑142 (MQ=255)

                                                                                                                                                                                                                                                                         tCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                                                                                            >  6:264603/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                         tCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                                                                                                            >  6:295974/1‑149 (MQ=255)

CGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGT‑ATGTACTCCCTCAC‑TCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGG  >  NC_000913/3780785‑3781320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`