| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,082,696 | G→C | 5.8% | Q22E (CAG→GAG) | fdoH ← | formate dehydrogenase‑O, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,082,696 | 0 | G | C | 5.8% | 408.8 / 14.2 | 123 | Q22E (CAG→GAG) | fdoH | formate dehydrogenase‑O, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base G (56/56); new base C (4/3); total (61/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-02 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGT > NC_000913/4082424‑4082960 | gCACGCTTTCAGGCGGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg < 2:205877/275‑1 (MQ=255) tGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACCGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTTGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGTCCGTTAGTCACGTAacg > 2:482013/1‑275 (MQ=255) cATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGACGTCCCGCGCCTGCGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac > 2:318618/1‑275 (MQ=255) gCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGa > 2:336547/1‑275 (MQ=255) ccGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAAAGTCGATGAGGTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACTAATGat > 2:297526/1‑275 (MQ=255) cGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTTGGGTTGTAGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTGGTTCCACTCTNCACACGCCCCCTAACAGGC‑TTACACGCCGATACAGGGTGTCACGTGGCTGAGTTTCCCCACTTCTTCCCGGACGNTCGGCGCCTTCGGCCCGTGGGTCACCCCGTNNCGCGCGGAACGACGCATggccaattgata > 3:74052/1‑264 (MQ=255) cGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTc < 1:121400/237‑1 (MQ=255) catccATTCCAGTTTTTCGTTCTGCTCAACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt < 4:183629/272‑1 (MQ=255) cAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt < 2:229390/275‑1 (MQ=255) ccAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGGTTGATAAGCCat > 2:453208/1‑274 (MQ=255) tgtttgatCCACTTCCGGGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTTTCGTACACCCCAATGTTATTGCCGACGGTATCGCGGATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgtc < 4:450453/265‑1 (MQ=255) gaAGCGCATTACCGTCCACGATTTGGCGCTTAAATCAGTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAGTGTCGTTCCACGCTGAACACGCCATCTGACAGGCGTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCcacag < 1:74052/275‑2 (MQ=255) ccGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaag < 1:86547/275‑1 (MQ=255) aTTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAg < 7:130842/149‑1 (MQ=255) gTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTCAGGCGCgg < 5:78036/149‑1 (MQ=255) ttGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCggg < 5:26418/149‑1 (MQ=255) gTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTCTTcc > 2:464446/1‑226 (MQ=255) tACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTCTTCCACAt < 4:464441/226‑1 (MQ=255) cacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAc < 5:53465/149‑1 (MQ=255) cacCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgt > 2:118499/1‑199 (MQ=255) acCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGAcc > 8:177155/1‑149 (MQ=255) cccAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCa < 5:179042/143‑1 (MQ=255) cccAATGTTATTGCCGACGGTATCGCGAATGCCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCa > 6:179042/1‑143 (MQ=255) cAATGTTCTTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACAAGCCACCTGACACGCTTTACA‑GCCGATACAGGTCGTAACGTCGATGAGTTTCGCCACTTCTGCCGGGAAGCCCCGCGCCCGAGGCGCGGGGGTCATACCGGTACTCGCGGAACGACGAATGATATCTTTAGATTGAGCACCCATACTGCCTCTCCGTTACCCCTTCTCCACATTCCCAACGAACGCCTTAAACTCCGGCTTTCGCGTCTTCGCATCACCAACGAAtctc > 4:155510/1‑272 (MQ=255) cAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTa > 6:320216/1‑149 (MQ=255) cAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTa < 7:115949/149‑1 (MQ=255) aaTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACCGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGACAGACGAATGTTATCTTGCGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCCCATTCCCAAGGAAGGACTTAAACTCCGGCGTCTTCGTTTTCCCCTCACCAACTAATGGCg > 1:84935/1‑275 (MQ=39) aTGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGt < 7:177233/149‑1 (MQ=255) gtttcatGCCGACGGTAGGGGGGATGTCGTGCCACGCTGGATCCGCGACCTGACAGGGCTTACG‑GCCGGATCGGTTGGTAACGTGGATCTGTTTCGGCAATTCTTCGTGGAAGTCCCGCGCCTGACGCGCGGGGGTGAGACCGTTAATCGCGGAACGGCGAGTGATGTCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACAGCTTTTCCACATTCACAAGGAAGGACTTGAACTCCGTCGTCTGCGTGTTCGCATCACCAGCGAATGGCGtt < 4:138929/269‑1 (MQ=255) gACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac > 6:8037/1‑149 (MQ=255) gACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAacgac > 8:288921/1‑149 (MQ=255) gACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt > 3:134978/1‑166 (MQ=255) gTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt < 1:134978/166‑1 (MQ=255) aaTGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCtt < 7:78330/149‑1 (MQ=255) tGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga < 8:252543/149‑1 (MQ=255) tGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTga < 5:51360/149‑1 (MQ=255) gTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTc > 3:112862/1‑124 (MQ=255) gTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGCTTCGCCACTTCTTACTGGAAGTCCCGCGCCCGACGCGCGGGGGTCAGACCTCTACTCGCGGAACGGCGAATGATGTCTTGCGATTGATAACCACTATGTTGTCCCCGTTACACATTTTCCACACGAACAAGGAAGGACTTAAACTCAGGCGTCTGCCTTTTAGCATCACCATCACATGGCGTTAACGTATTGGCAATaatgtatattt > 4:225968/1‑266 (MQ=25) cGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGACGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAt < 5:42063/149‑1 (MQ=255) gTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGAtt < 7:177155/149‑1 (MQ=255) ttCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCgg < 1:112862/124‑1 (MQ=255) ttCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAAc > 2:169807/1‑236 (MQ=255) tctGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGgcg < 7:329387/94‑1 (MQ=255) tGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGtt > 2:124676/1‑164 (MQ=255) tGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCtt > 2:362208/1‑275 (MQ=255) cacGCCACCTGCCAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTacac < 4:124675/164‑1 (MQ=255) tgcaaCCTGCCAGGGCTTACA‑CCCGGTACAGGTGGTTACGTCGATTGGTTACGCCACTTCTTCCTCGAATGCCCGCGCCTTCGGCGCGGGGGTCAGACCGTTAATCGCGGAACGACGAATGATATCTTGATATTGATAAGCCATATGTCCTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGc < 4:309936/271‑1 (MQ=255) gCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGTCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAACGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTACTAGcc > 2:200710/1‑275 (MQ=255) aCCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTa < 5:332072/99‑1 (MQ=255) aCCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTa > 6:332072/1‑99 (MQ=255) aCCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGtct > 8:358119/1‑149 (MQ=255) aCCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTGCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGtct < 7:358119/149‑1 (MQ=255) cTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAAc > 2:427527/1‑216 (MQ=255) cTGACAGGCTTTACA‑GCCGATACAGGAGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTATCCGTTACACCTTTTCCACATTCACAAGGAAGTACTTAAATTCCGGCCTCTGCGTGTTCGCATCCACAACGAATGGGGTTAAAGTATTTGCAATAAAACCTTTTTTAGCTAAGCCTTCATAgactgtac > 4:384756/1‑268 (MQ=255) aGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTac > 7:293644/1‑149 (MQ=255) gCTTTACC‑GCCGAGACATGTGGTAACGTCGATGAGTTTCGTCACTTCTTCCTGGAAGTCACGCGACTGAGGCGCGGGGGTCAGACCGTTAGTCGCCGAACGCCGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCgg < 1:70585/275‑1 (MQ=255) gCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTacac > 7:334121/1‑149 (MQ=255) gCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAAACgg > 1:57661/1‑275 (MQ=255) tttACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCt > 5:221050/1‑149 (MQ=255) ttACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCtt < 8:446099/149‑1 (MQ=255) aCA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCtttt < 5:8037/149‑1 (MQ=255) a‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGAt < 2:454/117‑1 (MQ=255) gATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTaca > 7:189361/1‑137 (MQ=255) gtcccgggggtAACGTCGATGACTTTCGCCACTTCTTCCGTGAAGTACCCCGCCTGAGGCGCGGGGGTAAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGAGGGt < 4:283309/267‑1 (MQ=255) aTACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCCCCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCTATgg > 2:449494/1‑274 (MQ=255) atgtggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTcaca > 5:110795/3‑149 (MQ=255) aGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTcaca > 5:371995/1‑149 (MQ=255) tggtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGa > 4:375211/1‑273 (MQ=255) gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa > 2:63895/1‑187 (MQ=255) gtAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa > 2:317828/1‑187 (MQ=255) aaCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAACGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCtt > 2:38202/1‑275 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAaggaagga < 6:110795/149‑1 (MQ=255) aCGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAaggaagga < 6:371995/149‑1 (MQ=255) cGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCa < 2:210832/176‑1 (MQ=255) cGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGaa < 4:63894/187‑1 (MQ=255) tCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtcgtc < 2:525417/112‑1 (MQ=255) gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTaa > 8:116653/1‑149 (MQ=255) gATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCCCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCCGCGTGGTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGa > 2:283313/1‑254 (MQ=255) aGTGTCGGCACTCTTTCCTGGAAGTCCCGCGCCGTGGGCGCGGGGGTCAGACCGGTATTCGCGGAACGGCGAATAATATCTTGCGCTTGATGGGCCACTTTTCGTCTCCGTTACACCTTTACCGCATTCACAAGGAAGGACTTAATCTCCCGCGTCTGCATGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTGTATCTACGCATTCATAGCCCCAGTAAATCGTAATACCGATCATATCGATATCTTTGCAGtt < 4:297522/272‑1 (MQ=255) tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCg < 6:221050/149‑1 (MQ=255) tttCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCGTTTCCACATTCACAAGGAAGGACTTAAACTCCg > 8:417736/1‑149 (MQ=255) cGCCACGTCGTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTGCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCa < 2:155512/275‑1 (MQ=255) cttcttCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaaggaag > 6:219833/1‑128 (MQ=255) ttcttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACaagg > 6:62024/1‑124 (MQ=255) ttcCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtgt < 5:320216/149‑1 (MQ=255) tGGAAGTCCCGCGCCTGAGGCGAGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATTTCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGc > 6:99984/1‑149 (MQ=255) ggAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAaggaagga > 6:353111/1‑121 (MQ=255) tgacccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtgt < 6:215733/138‑1 (MQ=255) tgacccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCgtgt > 5:215733/6‑143 (MQ=255) aaGTCCCGGGCCATAGAGGTGGGGGTCNNAGCGTTAGTCGGTGAACGACGAATGTTAGTNTTAGTGTGATAAGCCATCTTTCGGCTCCGTTACACCTTTTCCACATTCACAAGGAAGGAGTTAAACTCCGGCGTNTGCGTGTTCGCATCACCAACGAATAGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGt < 3:65184/273‑1 (MQ=255) tCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGt < 2:375216/275‑1 (MQ=255) ccccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCAcc > 8:326891/2‑149 (MQ=255) ccGCGCCTCAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTACACTCCGGCGTCTGCGTGTTCGCATCACCaa > 7:47626/1‑149 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa < 7:246404/148‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCaa > 8:246404/1‑148 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAAc < 7:116653/149‑1 (MQ=255) cgcgCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGc > 1:56043/1‑156 (MQ=255) cgcCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATgtc > 4:534042/1‑74 (MQ=255) tGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGtct < 2:534047/74‑1 (MQ=255) gAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGc < 8:334121/149‑1 (MQ=255) | GCACGCTTTCAGGCAGCCTGGATCGGAACAGTGCATACAGCCGTCTTTGCGGATCAGCCATTCCAGTTTGTCGTTCTGCTCCACTTCCGAGAAGCGCATTACCGTCCACGATTTGGCGCTTAAATCATTGGGGTTGTCGTACACCCCAATGTTATTGCCGACGGTATCGCGAATGTCGTTCCACTCTGAACACGCCACCTGACAGGCTTTACA‑GCCGATACAGGTGGTAACGTCGATGAGTTTCGCCACTTCTTCCTGGAAGTCCCGCGCCTGAGGCGCGGGGGTCAGACCGTTAGTCGCGGAACGACGAATGATATCTTGAGATTGATAAGCCATATGTCGTCTCCGTTACACCTTTTCCACATTCACAAGGAAGGACTTAAACTCCGGCGTCTGCGTGTTCGCATCACCAACGAATGGCGTTAAAGTATTGGCAATAAAGCCTTTTTTAGCTACGCCTTCATAGCCCCAGTGAATCGGAATACCGATGGTATCGATATCTTTGCCGTTCGCTTTCAGCGTGCGAATACGTTTGGT > NC_000913/4082424‑4082960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |